FastQCFastQC Report
Fri 27 May 2016
SRR2139042_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139042_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14240618
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA189700.050.614171
GTATCAA356250.036.637521
TCAACGC446450.028.7125034
ATCAACG447050.028.6739653
CAACGCA455150.028.1543645
TATCAAC463750.027.8156552
AACGCAG464700.027.639626
ACGCAGA543050.023.5815877
CGCAGAG546600.023.4284298
GTACATG413200.020.5387971
GCAGAGT628950.020.3072539
TACATGG411850.020.2149942
ACATGGG416150.019.6695523
AGAGTAC573600.019.24891710-11
GAGTACT391000.018.93576612-13
GTGGTAT89950.018.2852881
CAGAGTA616600.017.94436510-11
GGACCGA20800.017.9341756
AGTACTT426150.017.72704312-13
CATGGGG329850.017.3620474
Produced by FastQC (version 0.11.5)