##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139042_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14240618
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.590595857567415	34.0	31.0	34.0	31.0	34.0
2	32.7192120454323	34.0	31.0	34.0	31.0	34.0
3	32.78425908201456	34.0	31.0	34.0	31.0	34.0
4	36.268086890611066	37.0	37.0	37.0	35.0	37.0
5	36.20677178476384	37.0	37.0	37.0	35.0	37.0
6	36.24321212745121	37.0	37.0	37.0	35.0	37.0
7	36.1913332693848	37.0	37.0	37.0	35.0	37.0
8	36.17074006198327	37.0	37.0	37.0	35.0	37.0
9	37.88699528349121	39.0	38.0	39.0	35.0	39.0
10-11	37.83709221748663	39.0	38.0	39.0	35.0	39.0
12-13	37.80710805528243	39.0	38.0	39.0	35.0	39.0
14-15	39.1803660136098	40.0	39.0	41.0	36.0	41.0
16-17	39.15222148364629	40.0	39.0	41.0	36.0	41.0
18-19	39.166062385775675	40.0	39.0	41.0	36.0	41.0
20-21	39.16128654654925	40.0	39.0	41.0	36.0	41.0
22-23	39.02839184262669	40.0	39.0	41.0	35.5	41.0
24-25	38.97063238124533	40.0	38.0	41.0	35.0	41.0
26-27	38.912244962764134	40.0	38.0	41.0	35.0	41.0
28-29	38.720430172079446	40.0	38.0	41.0	35.0	41.0
30-31	38.60660069548724	40.0	38.0	41.0	35.0	41.0
32-33	38.488804608692845	40.0	38.0	41.0	34.0	41.0
34-35	38.20568597331974	40.0	38.0	41.0	34.0	41.0
36-37	38.456316985342006	40.0	38.0	41.0	34.0	41.0
38-39	38.55587295912699	40.0	38.0	41.0	34.0	41.0
40-41	38.551491525396315	40.0	38.0	41.0	34.0	41.0
42-43	38.490748759971936	40.0	38.0	41.0	34.0	41.0
44-45	38.43786615895148	40.0	38.0	41.0	34.0	41.0
46-47	38.36460143371703	40.0	38.0	41.0	34.0	41.0
48-49	38.2975599804126	40.0	38.0	41.0	34.0	41.0
50-51	38.2229830083205	40.0	38.0	41.0	33.5	41.0
52-53	38.11080422208588	40.0	37.0	41.0	33.0	41.0
54-55	37.9636739309069	40.0	37.0	41.0	33.0	41.0
56-57	37.81939764320774	40.0	37.0	41.0	33.0	41.0
58-59	37.64904541517299	39.0	36.0	41.0	33.0	41.0
60-61	37.452373727173466	39.0	36.0	41.0	33.0	41.0
62-63	37.21702379754403	39.0	35.0	40.0	32.0	41.0
64-65	36.93529797261318	38.0	35.0	40.0	32.0	41.0
66-67	36.65384475146855	38.0	35.0	40.0	31.5	41.0
68-69	36.37085597477355	37.0	35.0	40.0	31.0	41.0
70-71	36.068099320033724	37.0	35.0	39.5	31.0	41.0
72-73	35.76942271056281	36.0	34.5	39.0	31.0	40.5
74-75	35.46023189394626	36.0	34.0	39.0	31.0	40.0
76-77	34.42443856882197	35.0	33.5	37.0	29.5	39.0
78-79	34.717616903348464	35.0	34.0	37.0	30.0	39.0
80-81	34.531989032293595	35.0	34.0	37.0	30.0	39.0
82-83	34.29442411442915	35.0	34.0	36.0	30.0	38.5
84-85	34.04333505760175	35.0	34.0	36.0	30.0	37.0
86-87	33.80428626064089	35.0	33.5	35.5	30.0	37.0
88-89	33.61205096810173	35.0	33.0	35.0	30.0	36.5
90-91	33.44488326391091	35.0	33.0	35.0	29.5	36.0
92-93	33.317134096843844	35.0	33.0	35.0	29.0	36.0
94-95	33.246238555952466	34.0	33.0	35.0	29.0	35.5
96-97	33.198592791651016	34.0	33.0	35.0	29.0	35.0
98-99	33.218758690415115	34.0	33.0	35.0	29.5	35.0
100-101	32.173761142706184	33.0	31.0	34.5	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	16.0
20	91.0
21	482.0
22	2178.0
23	7279.0
24	17482.0
25	34707.0
26	60410.0
27	93453.0
28	131947.0
29	174623.0
30	223617.0
31	280715.0
32	354732.0
33	463998.0
34	651519.0
35	947045.0
36	1620125.0
37	3217852.0
38	4052408.0
39	1832029.0
40	73907.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.19644190978523	21.96300904658836	15.51013555100878	24.33041349261763
2	13.474162064624789	22.394686728999105	41.46519435033438	22.665956856041724
3	16.392420494287745	27.67112115687163	31.916858629390365	24.01959971945026
4	11.81565377399027	19.366885578061684	39.640817529184304	29.176643118763746
5	12.524941861718492	38.42523817612678	36.17329221058329	12.876527751571437
6	28.44697670051796	39.37028852050442	19.258737174098567	12.923997604879059
7	24.993762559585726	33.49603461703941	24.288540306926308	17.22166251644855
8	23.855230108423	37.118661956476174	21.749885345525506	17.276222589575323
9	25.394895081098305	16.68975321155304	21.816525097436077	36.09882660991258
10-11	23.514365738902622	27.20609105587974	29.65813702748013	19.621406177737512
12-13	24.487753972475073	24.956265942952758	29.68180173079567	20.8741783537765
14-15	21.706951892236226	26.145543787608577	27.466520969724524	24.680983350430672
16-17	21.0051810680748	29.232650739256975	28.099970853661187	21.662197339007033
18-19	21.085916492183234	28.399972697793007	29.93566917477605	20.57844163524771
20-21	21.959487217012132	27.489923173281532	29.76604163819659	20.784547971509745
22-23	21.75746948497517	27.469890560450377	29.540875709532816	21.23176424504163
24-25	21.46283533792165	27.63567423696952	29.6538612015053	21.24762922360353
26-27	21.443603013827698	27.80228112295112	29.53614753480471	21.21796832841648
28-29	21.3365231447938	27.93052371580101	29.548095183535484	21.184857955869703
30-31	21.634750067280205	27.715475012378853	29.466155407739237	21.183619512601705
32-33	21.44022363579443	27.87038288906635	29.403942224493168	21.28545125064605
34-35	21.562493217504986	27.799491218894325	29.432914616668633	21.205100946932056
36-37	21.51874512823884	27.878309062393548	29.36719326509524	21.235752544272373
38-39	21.443673286318997	27.897926461940937	29.367959735618538	21.29044051612152
40-41	21.597912315266996	27.866514498196928	29.193275223845838	21.34229796269024
42-43	21.55472999940535	27.96270301695305	28.972061823580862	21.510505160060735
44-45	21.676831642891155	28.069816347544368	28.762778330931393	21.49057367863308
46-47	21.737757785611127	27.998461847361156	28.488486977111315	21.7752933899164
48-49	21.680414594678755	28.215541421226725	28.386080130791097	21.717963853303424
50-51	21.646055097854052	28.3070262366762	28.27029136519561	21.776627300274136
52-53	21.774466686612968	28.19633873112526	28.147584064130136	21.881610518131634
54-55	21.792700575402417	28.150223232819283	28.15673526867613	21.900340923102178
56-57	21.85822581849873	28.192544332450943	28.09466321022463	21.8545666388257
58-59	21.75528935907843	28.367552237831887	28.161253611162767	21.71590479192692
60-61	21.925467086556345	28.270933645164327	28.104023163537523	21.6995761047418
62-63	21.816609103188924	28.362826288874736	28.028795724586946	21.7917688833494
64-65	21.799239408278044	28.34393467169659	28.01540681265781	21.841419107367553
66-67	21.939619112493823	28.340584619806748	27.834276759340792	21.885519508358637
68-69	21.81447772283595	28.270560613029232	27.979431951218842	21.935529712915976
70-71	21.828938204085933	28.13072016347384	28.015540136087157	22.024801496353074
72-73	21.889101142145993	28.102937438677465	27.953467402486034	22.054494016690505
74-75	21.78230224903725	28.21896530381419	27.92212053537363	22.07661191177493
76-77	21.963858729960368	28.143667368102456	27.855957942147302	22.036515959789874
78-79	21.92703970003908	28.14776534431506	27.79146287024724	22.13373208539862
80-81	21.92969684984782	28.121510323143035	27.845457867117407	22.103334959891736
82-83	21.89314189514523	28.10637794441716	27.941531752698523	22.058948407739084
84-85	22.0610786606237	28.100008381838006	27.864862263713867	21.97405069382442
86-87	21.88302243862388	28.105847929507682	27.907115252776265	22.10401437909218
88-89	21.922298751335624	28.036432679195293	27.926928784274647	22.11433978519444
90-91	21.995527156339588	28.0752630643452	27.922638738389434	22.00657104092578
92-93	22.113053044332656	27.96034646917404	27.89043172118365	22.036168765309654
94-95	21.881930173842225	28.01097276513876	27.988564212128473	22.118532848890542
96-97	21.80540494276747	28.02485044137761	28.12587699238554	22.043867623469385
98-99	22.057861423486365	28.625523795614328	28.103246952633004	21.21336782826631
100-101	22.619053958008735	31.265195730031692	25.318875926907698	20.79687438505187
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4390.0
1	3925.0
2	5285.5
3	7465.5
4	9681.0
5	13988.5
6	17603.0
7	21486.0
8	24843.5
9	25363.5
10	23292.5
11	20843.5
12	22335.0
13	29444.0
14	47095.5
15	71496.0
16	90558.0
17	99599.5
18	99868.0
19	94449.0
20	86745.5
21	79909.5
22	77434.0
23	83446.0
24	99094.0
25	124363.0
26	155921.5
27	188663.0
28	222409.0
29	257289.5
30	287845.0
31	318413.0
32	353207.5
33	384591.5
34	415465.5
35	445705.5
36	475160.0
37	498982.0
38	512862.5
39	520534.5
40	528395.0
41	539872.5
42	546151.0
43	551324.0
44	560227.0
45	566797.0
46	570233.5
47	569474.0
48	563396.0
49	554403.0
50	541368.5
51	522041.0
52	495257.5
53	465879.5
54	438226.0
55	405976.0
56	370348.5
57	331487.0
58	291812.5
59	252055.5
60	210137.5
61	171747.5
62	134240.0
63	101646.5
64	76612.5
65	58334.5
66	45153.0
67	34417.5
68	26167.0
69	18514.5
70	12521.0
71	9047.5
72	6506.5
73	4621.5
74	3265.0
75	2246.0
76	1479.5
77	912.0
78	565.5
79	357.0
80	251.0
81	179.0
82	122.5
83	78.0
84	48.5
85	30.5
86	26.0
87	22.0
88	15.0
89	10.5
90	8.0
91	6.0
92	5.0
93	2.5
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	7.022167155947867E-6
2	0.0014044334311895735
3	4.21330029356872E-5
4	7.022167155947867E-6
5	7.022167155947867E-6
6	7.022167155947867E-6
7	7.022167155947867E-6
8	3.511083577973933E-5
9	0.0
10-11	0.0
12-13	0.0
14-15	6.987056320168128E-4
16-17	0.002861533116048756
18-19	7.724383871542654E-5
20-21	7.022423088491169E-6
22-23	9.691814009453945E-4
24-25	0.005584804162344005
26-27	0.009515927107295558
28-29	0.36130518919800747
30-31	0.33038610542976615
32-33	0.3339896429584841
34-35	0.18881149005754821
36-37	0.04747845255872165
38-39	0.03783043408518176
40-41	0.02270915255565669
42-43	0.03042390704300564
44-45	0.019105366314997527
46-47	0.01440422162525672
48-49	0.009034941476877112
50-51	0.004928434117609722
52-53	0.0033940284808454085
54-55	4.6788260150550044E-4
56-57	0.0026147365546552465
58-59	0.005132611257545573
60-61	0.003683683115789192
62-63	0.0033922492394084076
64-65	3.7341241003621743E-4
66-67	9.738162715447722E-5
68-69	6.399833801239131E-4
70-71	9.416415766199439E-4
72-73	0.0016228702763047772
74-75	0.0019415649980427498
76-77	8.745542399182651E-4
78-79	1.0408552603588842E-4
80-81	0.0033630605642425353
82-83	0.0015782802320578769
84-85	7.652585234606881E-5
86-87	4.560389822121995E-6
88-89	0.00144264514536037
90-91	0.003609197078962755
92-93	0.015849473595244412
94-95	0.0016695719159366658
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	329.0
20-21	1005.0
22-23	2252.0
24-25	6543.0
26-27	7485.0
28-29	11864.0
30-31	31349.0
32-33	40858.0
34-35	58136.0
36-37	79642.0
38-39	94458.0
40-41	101797.0
42-43	119553.0
44-45	142814.0
46-47	140321.0
48-49	124184.0
50-51	112198.0
52-53	101639.0
54-55	108522.0
56-57	115762.0
58-59	119011.0
60-61	119216.0
62-63	118015.0
64-65	126986.0
66-67	135245.0
68-69	134624.0
70-71	131624.0
72-73	128147.0
74-75	131405.0
76-77	132178.0
78-79	139203.0
80-81	142821.0
82-83	139227.0
84-85	141762.0
86-87	148468.0
88-89	157052.0
90-91	169503.0
92-93	175089.0
94-95	197117.0
96-97	284646.0
98-99	1180510.0
100-101	8688058.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.08819145215858
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.90995306584006	71.28459104753114
2	7.783593087311067	12.623149728991557
3	2.1786980525504562	5.300000544049693
4	0.9104334872492663	2.9530161967409954
5	0.4508487981227307	1.8279256829075792
6	0.2485934880398703	1.209479781116214
7	0.1508431359787855	0.8562117962644222
8	0.10018302006332763	0.6498927928920444
9	0.0631164323655252	0.46061976162896323
>10	0.19927129661201048	2.4702399460872635
>50	0.003434767040128615	0.1904383524985787
>100	9.645669648051473E-4	0.11720570533057095
>500	3.3401467357022116E-5	0.02067910843475945
>1k	3.340039453494467E-5	0.03654955552622685
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	4.21330029356872E-5	0.0	0.0	0.0
3	0.0	4.915517009163507E-5	0.0	0.0	0.0
4	0.0	1.5448767743085306E-4	0.0	0.0	0.0
5	0.0	1.5448767743085306E-4	0.0	0.0	0.0
6	0.0	1.6150984458680094E-4	0.0	0.0	0.0
7	0.0	1.6150984458680094E-4	0.0	7.022167155947867E-6	0.0
8	0.0	2.0364284752248814E-4	0.0	1.4044334311895734E-5	0.0
9	0.0	2.949310205498104E-4	0.0	2.10665014678436E-5	0.0
10-11	0.0	2.949310205498104E-4	0.0	2.10665014678436E-5	0.0
12-13	0.0	3.0195318770575826E-4	0.0	3.5110835779739336E-5	0.0
14-15	0.0	4.4239653082471563E-4	0.0	3.5110835779739336E-5	0.0
16-17	7.022167155947867E-6	4.5644086513661134E-4	0.0	4.21330029356872E-5	0.0
18-19	7.022167155947867E-6	4.915517009163507E-4	0.0	7.022167155947866E-5	0.0
20-21	7.022167155947867E-6	5.30173620274064E-4	0.0	1.158657580731398E-4	0.0
22-23	7.022167155947867E-6	5.723066232097512E-4	0.0	3.7217485926523694E-4	0.0
24-25	7.022167155947867E-6	6.039063754115165E-4	0.0	0.0011481243299974763	0.0
26-27	7.022167155947867E-6	6.249728768793601E-4	0.0	0.00368663775687263	0.0
28-29	7.022167155947867E-6	6.249728768793601E-4	0.0	0.01821550160252877	0.0
30-31	7.022167155947867E-6	7.092388827507345E-4	0.0	0.02580646429810841	0.0
32-33	7.022167155947867E-6	7.619051364203436E-4	0.0	0.029040172273422406	0.0
34-35	7.022167155947867E-6	8.567043930256397E-4	0.0	0.03335178290717439	0.0
36-37	7.022167155947867E-6	9.51503649630936E-4	0.0	0.03938733557771158	0.0
38-39	7.022167155947867E-6	9.690590675208057E-4	0.0	0.05086857887768635	0.0
40-41	7.022167155947867E-6	0.0010322585719243363	0.0	0.07956115387688933	0.0
42-43	7.022167155947867E-6	0.0010638583241261018	0.0	0.09084577649649755	0.0
44-45	7.022167155947867E-6	0.0011691908314653198	0.0	0.10756555649480942	0.0
46-47	7.022167155947867E-6	0.0012499457537587203	0.0	0.12674309499770303	0.0
48-49	7.022167155947867E-6	0.0012499457537587203	0.0	0.1429537678772087	0.0
50-51	7.022167155947867E-6	0.0013868780132997036	0.0	0.15830773636368872	0.0
52-53	7.022167155947867E-6	0.0013939001804556517	0.0	0.18321887434941375	0.0
54-55	7.022167155947867E-6	0.001418477765501469	0.0	0.23701920801470833	0.0
56-57	7.022167155947867E-6	0.0014676329355931043	0.0	0.2919325551742207	0.0
58-59	7.022167155947867E-6	0.001478166186327026	0.0	0.3312988242504644	0.0
60-61	7.022167155947867E-6	0.0015202991892627133	0.0	0.35343620620959004	0.0
62-63	7.022167155947867E-6	0.0015589211086204265	0.0	0.3672839198411193	0.0
64-65	7.022167155947867E-6	0.0017309642039411493	0.0	0.37715357577880393	0.0
66-67	7.022167155947867E-6	0.0017625639561429146	0.0	0.3832804166223685	0.0
68-69	7.022167155947867E-6	0.0018362967112803671	0.0	0.3891158375289612	0.0
70-71	7.022167155947867E-6	0.0018749186306380805	0.0	0.3943052190572066	0.0
72-73	7.022167155947867E-6	0.001899496215683898	0.0	0.3985009639328855	0.0
74-75	7.022167155947867E-6	0.0019205627171517416	0.0	0.4019769366750797	0.0
76-77	7.022167155947867E-6	0.0019837622215552724	0.0	0.4050561569729628	0.0
78-79	7.022167155947867E-6	0.0020294063080689333	0.0	0.40772809157580103	0.0
80-81	7.022167155947867E-6	0.0020434506423808296	0.0	0.4090342146668073	0.0
82-83	7.022167155947867E-6	0.002053983893114751	0.0	0.40955034395276946	0.0
84-85	7.022167155947867E-6	0.0020785614781605685	0.0	0.40994007422992457	7.022167155947867E-6
86-87	7.022167155947867E-6	0.0020926058124724643	0.0	0.41005242890441973	7.022167155947867E-6
88-89	7.022167155947867E-6	0.002176871818343839	0.0	0.41008753974019946	7.022167155947867E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	18970	0.0	50.61417	1
GTATCAA	35625	0.0	36.63752	1
TCAACGC	44645	0.0	28.712503	4
ATCAACG	44705	0.0	28.673965	3
CAACGCA	45515	0.0	28.154364	5
TATCAAC	46375	0.0	27.815655	2
AACGCAG	46470	0.0	27.63962	6
ACGCAGA	54305	0.0	23.581587	7
CGCAGAG	54660	0.0	23.428429	8
GTACATG	41320	0.0	20.538797	1
GCAGAGT	62895	0.0	20.307253	9
TACATGG	41185	0.0	20.214994	2
ACATGGG	41615	0.0	19.669552	3
AGAGTAC	57360	0.0	19.248917	10-11
GAGTACT	39100	0.0	18.935766	12-13
GTGGTAT	8995	0.0	18.285288	1
CAGAGTA	61660	0.0	17.944365	10-11
GGACCGA	2080	0.0	17.934175	6
AGTACTT	42615	0.0	17.727043	12-13
CATGGGG	32985	0.0	17.362047	4
>>END_MODULE