FastQCFastQC Report
Fri 27 May 2016
SRR2139041_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139041_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10389115
Sequences flagged as poor quality0
Sequence length19-101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA151200.042.9708561
GTATCAA265200.033.9157521
ATCAACG317350.027.867133
TCAACGC318550.027.8297334
CAACGCA322350.027.5016655
TATCAAC332750.026.8236452
AACGCAG335200.026.5116026
ACGCAGA381100.023.3185217
CGCAGAG381150.023.2928718
GCAGAGT439450.020.1245179
GTACATG266700.019.534351
GAGTACT268900.019.38365412-13
AGAGTAC386100.019.20414510-11
TACATGG266650.019.102042
ACATGGG268250.018.18533
CAGAGTA434900.017.43043710-11
CTTATAC136400.017.2982731
GTACTTT305750.017.0551714-15
AGTACTT301650.017.00082812-13
TATACAC190800.015.6155763
Produced by FastQC (version 0.11.5)