##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139041_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10389115
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.62951935751987	34.0	31.0	34.0	31.0	34.0
2	32.751000542394614	34.0	31.0	34.0	31.0	34.0
3	32.82030028544298	34.0	31.0	34.0	31.0	34.0
4	36.291436951078126	37.0	37.0	37.0	35.0	37.0
5	36.23263069087213	37.0	37.0	37.0	35.0	37.0
6	36.270811613886266	37.0	37.0	37.0	35.0	37.0
7	36.22233356739241	37.0	37.0	37.0	35.0	37.0
8	36.201554992894	37.0	37.0	37.0	35.0	37.0
9	37.93244573767833	39.0	38.0	39.0	35.0	39.0
10-11	37.884381008391955	39.0	38.0	39.0	35.0	39.0
12-13	37.86043724609844	39.0	38.0	39.0	35.0	39.0
14-15	39.2546481100652	41.0	39.0	41.0	36.0	41.0
16-17	39.22342398751	41.0	39.0	41.0	36.0	41.0
18-19	39.23562209100582	40.5	39.0	41.0	36.0	41.0
20-21	39.23326879221071	40.0	39.0	41.0	36.0	41.0
22-23	39.1019862813652	40.0	39.0	41.0	35.5	41.0
24-25	39.05166191773945	40.0	39.0	41.0	35.0	41.0
26-27	39.000612962759234	40.0	38.5	41.0	35.0	41.0
28-29	38.81091017009018	40.0	38.0	41.0	35.0	41.0
30-31	38.70501054740673	40.0	38.0	41.0	35.0	41.0
32-33	38.59126724155662	40.0	38.0	41.0	35.0	41.0
34-35	38.30983410582839	40.0	38.0	41.0	34.0	41.0
36-37	38.555641211595464	40.0	38.0	41.0	34.5	41.0
38-39	38.649423622714565	40.0	38.0	41.0	35.0	41.0
40-41	38.65066861332562	40.0	38.0	41.0	35.0	41.0
42-43	38.596648974451014	40.0	38.0	41.0	34.5	41.0
44-45	38.55053118944025	40.0	38.0	41.0	34.0	41.0
46-47	38.480280476497995	40.0	38.0	41.0	34.0	41.0
48-49	38.420270108126545	40.0	38.0	41.0	34.0	41.0
50-51	38.34687339030475	40.0	38.0	41.0	34.0	41.0
52-53	38.24938959113388	40.0	38.0	41.0	34.0	41.0
54-55	38.11692533571999	40.0	37.0	41.0	33.0	41.0
56-57	37.98230627573141	40.0	37.0	41.0	33.0	41.0
58-59	37.82750162269143	40.0	37.0	41.0	33.0	41.0
60-61	37.64144588821495	39.0	36.0	41.0	33.0	41.0
62-63	37.41889547858001	39.0	36.0	40.5	32.5	41.0
64-65	37.153082538509636	39.0	35.0	40.0	32.0	41.0
66-67	36.884647853075954	38.0	35.0	40.0	32.0	41.0
68-69	36.6085573650072	38.0	35.0	40.0	31.5	41.0
70-71	36.30316119126707	37.0	35.0	39.5	31.0	41.0
72-73	35.99692424984708	37.0	35.0	39.0	31.0	40.5
74-75	35.67947178542805	36.0	34.0	39.0	31.0	40.0
76-77	34.619344883108425	35.0	33.5	37.0	29.5	39.0
78-79	34.8999301245191	35.0	34.0	37.0	30.5	39.0
80-81	34.68804145329676	35.0	34.0	37.0	30.0	39.0
82-83	34.43006036389475	35.0	34.0	36.5	30.0	38.5
84-85	34.162124213979574	35.0	34.0	36.0	30.0	37.0
86-87	33.91182135975473	35.0	34.0	36.0	30.0	37.0
88-89	33.70891793423447	35.0	33.5	35.0	30.0	37.0
90-91	33.524760854749175	35.0	33.0	35.0	30.0	36.0
92-93	33.394576463055884	35.0	33.0	35.0	30.0	36.0
94-95	33.31314832374162	34.5	33.0	35.0	30.0	36.0
96-97	33.25816805030098	34.0	33.0	35.0	30.0	35.0
98-99	33.28087769098788	34.0	33.0	35.0	30.0	35.0
100-101	32.24890803531787	33.0	31.0	34.5	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	0.0
19	5.0
20	36.0
21	260.0
22	1279.0
23	4304.0
24	10510.0
25	22101.0
26	39142.0
27	61866.0
28	89109.0
29	120326.0
30	156772.0
31	197602.0
32	251892.0
33	329986.0
34	459318.0
35	663824.0
36	1126910.0
37	2308826.0
38	3162822.0
39	1331384.0
40	50839.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.991266627741304	22.056519930380976	15.622429400620689	24.329784041257028
2	13.235271185980604	22.28438138239944	42.07331617274632	22.407031258873637
3	16.444424423487895	27.566826199038726	31.913655004651986	24.075094372821397
4	11.468361158455009	19.661360888075816	40.006447133648464	28.863830819820713
5	12.339810690305256	38.414834989778726	36.46134790704963	12.784006412866391
6	28.016585437410736	39.74476553053513	19.4264592726579	12.812189759396231
7	24.74794289484166	33.47954406891675	24.288259807332945	17.484253228908646
8	23.321398075888524	37.50130786024246	21.941597097806383	17.235696966062626
9	24.811362661785918	17.089472972433168	22.005570253096632	36.093594112684286
10-11	23.044879183645577	27.466338566855793	29.93127422306905	19.557508026429584
12-13	24.14949685319683	25.121629705706404	29.818021073017288	20.910852368079475
14-15	21.344490083858993	26.543995158747247	27.505257580664406	24.606257176729358
16-17	20.81498150370441	29.65651015604538	28.14050311887654	21.388005221373675
18-19	20.746434272107734	28.752926260403562	29.93629003746518	20.564349430023526
20-21	21.56639211391883	27.88083900260044	29.827038843766463	20.725730039714268
22-23	21.37908411948645	27.937876596001733	29.6164445607125	21.066594723799316
24-25	21.144241313612543	28.047104502209745	29.776640496749874	21.03201368742784
26-27	21.18622852330579	28.185680543899178	29.63802343278997	20.990067500005058
28-29	20.94535491487209	28.193327692664322	29.67925456518478	21.182062827278802
30-31	21.299358328364416	28.103260877191396	29.67152487702378	20.925855917420407
32-33	21.262552046489695	28.08245529121213	29.691291016667286	20.963701645630888
34-35	21.13607090297244	28.189987538088378	29.754445935119726	20.919495623819458
36-37	21.233881394301346	28.17190812188025	29.537384464071863	21.056826019746538
38-39	21.01747066691653	28.255677289794846	29.633855901969774	21.092996141318856
40-41	21.222274869824954	28.15037177496167	29.569463826919844	21.057889528293533
42-43	21.190065999102046	28.222813712358736	29.366731874777873	21.22038841376134
44-45	21.238178469367504	28.415509477408186	29.103219730288245	21.243092322936064
46-47	21.434300087764253	28.25568387048143	28.72485276117458	21.585163280579742
48-49	21.349580408276385	28.39175534762552	28.734451551409784	21.524212692688312
50-51	21.25824754624478	28.545441813941387	28.666444159467126	21.5298664803467
52-53	21.330809233823537	28.548171709033028	28.672258057609906	21.448760999533526
54-55	21.381438214331787	28.48391343338792	28.525763736955454	21.608884615324836
56-57	21.537551464845684	28.642368972699273	28.318682884153873	21.50139667830117
58-59	21.333857038656916	28.777053159686737	28.58321096379165	21.305878837864693
60-61	21.478700612199777	28.747217977117224	28.37811207108943	21.395969339593567
62-63	21.459753101702546	28.848410891015146	28.38082909848399	21.311006908798312
64-65	21.334188827564713	28.779496989846297	28.34713508564317	21.539179096945816
66-67	21.378028552504983	28.745713172909156	28.239250960089475	21.637007314496387
68-69	21.39258165053433	28.75410391241297	28.359459571644773	21.493854865407926
70-71	21.3661604293776	28.557717421031136	28.443959700084108	21.632162449507156
72-73	21.386188919139492	28.555753609811603	28.287174505632983	21.77088296541592
74-75	21.38401786008755	28.62161819657933	28.222000318962404	21.772363624370715
76-77	21.44830059557726	28.432960774265826	28.30864963475978	21.810088995397138
78-79	21.405778978373164	28.34564826393447	28.370496903707494	21.87807585398488
80-81	21.584845266324763	28.411035220787856	28.172056925151363	21.832062587736022
82-83	21.646102931615093	28.525409375543465	28.286077294017176	21.542410398824266
84-85	21.59395290543161	28.32143418466182	28.17539755703257	21.909215352873996
86-87	21.411612293021438	28.537544646205866	28.261114428922856	21.78972863184984
88-89	21.720127693071415	28.25430315687	28.21030609195417	21.81526305810441
90-91	21.711313665203345	28.47307659409049	28.24020539599566	21.575404344710506
92-93	21.588890926322357	28.395594917408605	28.299083923926805	21.71643023234223
94-95	21.618204458935757	28.218428621445007	28.366778367016433	21.796588552602802
96-97	21.53006156221167	28.485833114109422	28.3975444422197	21.586560881459206
98-99	21.810648914304263	28.911069024299685	28.498051253231033	20.78023080816502
100-101	22.149500342409166	31.683103414213594	25.82925850819562	20.338137735181625
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2430.0
1	2125.0
2	2850.5
3	4100.0
4	5522.5
5	8313.5
6	10569.0
7	12692.0
8	14317.0
9	14277.0
10	13063.0
11	12162.5
12	13776.0
13	18880.0
14	31038.0
15	47681.5
16	60932.5
17	68092.0
18	68961.0
19	65221.5
20	59951.0
21	54901.0
22	53081.5
23	57740.0
24	68556.0
25	85648.0
26	107840.0
27	132286.0
28	158910.0
29	187780.0
30	214811.5
31	240569.0
32	267547.0
33	292601.5
34	315917.0
35	337722.0
36	361199.5
37	379927.0
38	391107.5
39	399444.5
40	407377.5
41	413850.0
42	416179.0
43	418122.0
44	420274.0
45	421979.0
46	421434.0
47	418588.5
48	411426.5
49	401977.5
50	390777.5
51	368766.5
52	339820.0
53	314676.0
54	290076.5
55	262808.0
56	235480.5
57	207586.0
58	179547.0
59	152196.0
60	124689.5
61	98692.5
62	75787.0
63	56785.5
64	41641.0
65	30665.0
66	22847.0
67	17138.0
68	12850.5
69	9125.0
70	6375.0
71	4479.0
72	3140.5
73	2150.5
74	1424.0
75	931.0
76	586.5
77	367.5
78	246.5
79	165.5
80	112.0
81	76.5
82	58.0
83	41.5
84	27.5
85	20.0
86	13.0
87	8.5
88	5.0
89	3.0
90	4.0
91	4.5
92	3.5
93	2.5
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	9.625458953914746E-6
2	0.001857713578105546
3	9.625458953914746E-6
4	1.925091790782949E-5
5	9.625458953914746E-6
6	9.625458953914746E-6
7	9.625458953914746E-6
8	7.700367163131797E-5
9	0.0
10-11	0.0
12-13	0.0
14-15	6.593439383431601E-4
16-17	0.0026758775891883
18-19	1.925091790782949E-5
20-21	0.0
22-23	0.0012033118028763484
24-25	0.005955012934220696
26-27	0.010671407802359364
28-29	0.35918995777129453
30-31	0.32864326382346326
32-33	0.3316093089489288
34-35	0.18753485426798383
36-37	0.04654777778847395
38-39	0.0369865634704087
40-41	0.022551151317340982
42-43	0.02987331578181266
44-45	0.018744880285886827
46-47	0.014254064444661648
48-49	0.008505182481048456
50-51	0.0045180890691172565
52-53	0.0032061431143033253
54-55	4.921712393260363E-4
56-57	0.0026830871600863953
58-59	0.005125916237792201
60-61	0.003957062486301713
62-63	0.003083999283316983
64-65	3.4449313460170323E-4
66-67	4.348106350768044E-5
68-69	5.655235641864578E-4
70-71	8.593155235848294E-4
72-73	0.001438056526476859
74-75	0.0018692383621699588
76-77	9.06415299651777E-4
78-79	1.4393306306542306E-4
80-81	0.003316446993751349
82-83	0.001476175005898819
84-85	6.538860477540114E-5
86-87	3.60631581300964E-5
88-89	0.0013929472586372159
90-91	0.0037528643321056926
92-93	0.014577367911200076
94-95	0.0016442122712323033
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	155.0
20-21	682.0
22-23	1366.0
24-25	3087.0
26-27	4881.0
28-29	7285.0
30-31	18028.0
32-33	24219.0
34-35	34271.0
36-37	47550.0
38-39	56064.0
40-41	61468.0
42-43	73749.0
44-45	88875.0
46-47	88590.0
48-49	78101.0
50-51	69463.0
52-53	63205.0
54-55	70122.0
56-57	77804.0
58-59	48290.0
60-61	81529.0
62-63	80719.0
64-65	85442.0
66-67	95105.0
68-69	88840.0
70-71	84222.0
72-73	81656.0
74-75	82943.0
76-77	84465.0
78-79	90338.0
80-81	92220.0
82-83	90571.0
84-85	91403.0
86-87	97026.0
88-89	103939.0
90-91	114043.0
92-93	118544.0
94-95	134409.0
96-97	202448.0
98-99	914904.0
100-101	6657094.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.01890947935638
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.70739598798973	74.53098673664329
2	7.774891122382339	13.064757468465865
3	1.9861139719591385	5.006133900771595
4	0.7416173339280191	2.492395185904794
5	0.3150385881429469	1.3234599309843245
6	0.17110698766774915	0.8625733504885179
7	0.10088477128408879	0.5933359926454398
8	0.05602228392146654	0.3765544961299599
9	0.03592078240041205	0.27162224684350894
>10	0.10941240978466966	1.3446797664568921
>50	0.0012328815882104427	0.06286288769558851
>100	3.446433191762962E-4	0.05789064951182908
>500	1.8235632016255602E-5	0.012747387458396302
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.925091790782949E-5	0.0	0.0	0.0
3	0.0	1.925091790782949E-5	0.0	9.625458953914746E-6	0.0
4	0.0	7.700367163131797E-5	0.0	9.625458953914746E-6	0.0
5	0.0	7.700367163131797E-5	0.0	9.625458953914746E-6	0.0
6	0.0	7.700367163131797E-5	0.0	9.625458953914746E-6	0.0
7	2.887637686174424E-5	7.700367163131797E-5	0.0	9.625458953914746E-6	0.0
8	3.850183581565898E-5	7.700367163131797E-5	0.0	9.625458953914746E-6	0.0
9	4.812729476957373E-5	9.625458953914746E-5	0.0	4.812729476957373E-5	0.0
10-11	4.812729476957373E-5	9.625458953914746E-5	0.0	5.2940024246531105E-5	0.0
12-13	5.7752753723488474E-5	9.625458953914746E-5	0.0	6.737821267740323E-5	0.0
14-15	6.737821267740323E-5	1.1550550744697696E-4	0.0	8.181640110827534E-5	0.0
16-17	8.181640110827534E-5	1.1550550744697696E-4	0.0	1.1069277797001959E-4	0.0
18-19	8.662913058523272E-5	1.3475642535480645E-4	0.0	1.7807099064742283E-4	0.0
20-21	8.662913058523272E-5	1.6363280221655068E-4	0.0	3.032019570483145E-4	0.0
22-23	1.0106731901610483E-4	1.7325826117046544E-4	0.0	6.112166435735864E-4	0.0
24-25	1.2031823692393434E-4	1.8288372012438016E-4	0.0	0.0012561223934858742	0.0
26-27	1.251309664008917E-4	1.9250917907829491E-4	0.0	0.0031667759958379513	0.0
28-29	1.251309664008917E-4	1.9250917907829491E-4	0.0	0.013326447921694965	0.0
30-31	1.251309664008917E-4	2.3582374437091129E-4	0.0	0.018529008486285886	0.0
32-33	1.251309664008917E-4	2.45449203324826E-4	0.0	0.02077655315202498	0.0
34-35	1.251309664008917E-4	3.032019570483145E-4	0.0	0.023640127190814618	0.0
36-37	1.251309664008917E-4	3.561419812948456E-4	0.0	0.027947520072691467	0.0
38-39	1.251309664008917E-4	3.561419812948456E-4	0.0	0.036591182213306914	0.0
40-41	1.251309664008917E-4	3.946438171105046E-4	0.0	0.05582766193270553	0.0
42-43	1.251309664008917E-4	4.3795838240312095E-4	0.0	0.06422587486999615	0.0
44-45	1.299436958778491E-4	5.6790207828097E-4	0.0	0.07579567653260166	0.0
46-47	1.8288372012438016E-4	6.641566678201175E-4	0.0	0.08919912812592795	0.0
48-49	1.9250917907829491E-4	6.641566678201175E-4	0.0	0.10136570824367619	0.0
50-51	1.9250917907829491E-4	7.796621752670944E-4	0.0	0.11294513536523562	0.0
52-53	1.9250917907829491E-4	7.892876342210092E-4	0.0	0.12942873382381465	0.0
54-55	2.0213463803220967E-4	7.941003636979665E-4	0.0	0.166756263647096	0.0
56-57	2.310110148939539E-4	7.98913093174924E-4	0.0	0.20578268697574337	0.0
58-59	2.502619328017834E-4	7.98913093174924E-4	0.0	0.23606438084475917	0.0
60-61	2.502619328017834E-4	8.085385521288387E-4	0.0	0.5437229253887361	0.0
62-63	2.502619328017834E-4	8.326021995136256E-4	0.0	0.5501334810520434	0.0
64-65	2.502619328017834E-4	9.336695185297304E-4	0.0	0.5566499167638437	0.0
66-67	2.502619328017834E-4	9.577331659145173E-4	0.0	0.5623578139235151	0.0
68-69	2.5507466227874074E-4	0.0010154859196380057	0.0	0.5683881639581427	0.0
70-71	2.598873917556981E-4	0.0010299241080688778	0.0	0.5740671847409524	0.0
72-73	2.647001212326555E-4	0.0010347368375458353	0.0	0.5782013193616589	0.0
74-75	2.695128507096129E-4	0.0010491750259767072	0.0	0.5816279827492525	0.0
76-77	2.695128507096129E-4	0.0010876768617923663	0.0	0.5846792532376435	0.0
78-79	2.695128507096129E-4	0.0010973023207462812	0.0	0.5872925653436313	0.0
80-81	2.743255801865703E-4	0.0011069277797001959	0.0	0.5886449423266563	0.0
82-83	2.7913830966352763E-4	0.0011069277797001959	0.0	0.5892176571344143	0.0
84-85	2.7913830966352763E-4	0.0011261786976080254	0.0	0.5895545481978013	0.0
86-87	2.887637686174424E-4	0.0011454296155158548	0.0	0.5896508027873404	0.0
88-89	2.887637686174424E-4	0.001174305992377599	0.0	0.5896844918936791	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15120	0.0	42.970856	1
GTATCAA	26520	0.0	33.915752	1
ATCAACG	31735	0.0	27.86713	3
TCAACGC	31855	0.0	27.829733	4
CAACGCA	32235	0.0	27.501665	5
TATCAAC	33275	0.0	26.823645	2
AACGCAG	33520	0.0	26.511602	6
ACGCAGA	38110	0.0	23.318521	7
CGCAGAG	38115	0.0	23.292871	8
GCAGAGT	43945	0.0	20.124517	9
GTACATG	26670	0.0	19.53435	1
GAGTACT	26890	0.0	19.383654	12-13
AGAGTAC	38610	0.0	19.204145	10-11
TACATGG	26665	0.0	19.10204	2
ACATGGG	26825	0.0	18.1853	3
CAGAGTA	43490	0.0	17.430437	10-11
CTTATAC	13640	0.0	17.298273	1
GTACTTT	30575	0.0	17.05517	14-15
AGTACTT	30165	0.0	17.000828	12-13
TATACAC	19080	0.0	15.615576	3
>>END_MODULE