FastQCFastQC Report
Fri 27 May 2016
SRR2139040_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139040_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9674458
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA131450.051.1883161
GTATCAA240950.036.5880361
ATCAACG300450.028.8856643
TCAACGC304250.028.4967164
CAACGCA308700.028.0998125
TATCAAC313200.027.942532
AACGCAG317800.027.362626
ACGCAGA360650.024.1115767
CGCAGAG362800.023.9214368
CGAGACC71500.022.9038488-89
GAGACCG73200.022.60658888-89
GACCGAG75100.022.1478690-91
ACGAGAC74850.022.03314886-87
ACCGAGG79100.021.74560792-93
AGACCGA76350.021.59415890-91
ATCTCCG75450.020.81209874-75
CACGAGA79850.020.6534986-87
GCCCACG79250.020.60211282-83
GCAGAGT427350.020.2382839
CCCACGA81300.019.74358684-85
Produced by FastQC (version 0.11.5)