##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139040_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9674458
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.67172042092694	34.0	31.0	34.0	31.0	34.0
2	32.78101491577099	34.0	31.0	34.0	31.0	34.0
3	32.849426293441965	34.0	31.0	34.0	31.0	34.0
4	36.31137144840569	37.0	37.0	37.0	35.0	37.0
5	36.25601082768668	37.0	37.0	37.0	35.0	37.0
6	36.29374565479534	37.0	37.0	37.0	35.0	37.0
7	36.2445650185261	37.0	37.0	37.0	35.0	37.0
8	36.223859879282124	37.0	37.0	37.0	35.0	37.0
9	37.959181072469384	39.0	38.0	39.0	35.0	39.0
10-11	37.91431561333978	39.0	38.0	39.0	35.0	39.0
12-13	37.88993316214717	39.0	38.0	39.0	35.0	39.0
14-15	39.292996103761055	41.0	39.0	41.0	36.0	41.0
16-17	39.26624385572815	41.0	39.0	41.0	36.0	41.0
18-19	39.27901035902993	41.0	39.0	41.0	36.0	41.0
20-21	39.27411466303879	41.0	39.0	41.0	36.0	41.0
22-23	39.14296952046166	40.0	39.0	41.0	36.0	41.0
24-25	39.092498127136324	40.0	39.0	41.0	35.5	41.0
26-27	39.04413735835878	40.0	39.0	41.0	35.0	41.0
28-29	38.856411455928125	40.0	38.5	41.0	35.0	41.0
30-31	38.74488583414166	40.0	38.0	41.0	35.0	41.0
32-33	38.63194309766372	40.0	38.0	41.0	35.0	41.0
34-35	38.35293018692187	40.0	38.0	41.0	34.0	41.0
36-37	38.60131371649289	40.0	38.0	41.0	34.5	41.0
38-39	38.69869175393623	40.0	38.0	41.0	35.0	41.0
40-41	38.691957705405734	40.0	38.0	41.0	35.0	41.0
42-43	38.634431366303794	40.0	38.0	41.0	35.0	41.0
44-45	38.58292815516146	40.0	38.0	41.0	34.5	41.0
46-47	38.51340873994853	40.0	38.0	41.0	34.0	41.0
48-49	38.44591214197898	40.0	38.0	41.0	34.0	41.0
50-51	38.36821881248471	40.0	38.0	41.0	34.0	41.0
52-53	38.25860705496872	40.0	37.5	41.0	34.0	41.0
54-55	38.11598936041926	40.0	37.0	41.0	33.5	41.0
56-57	37.973115678217134	40.0	37.0	41.0	33.0	41.0
58-59	37.802306221012	40.0	36.5	41.0	33.0	41.0
60-61	37.60726034911231	39.0	36.0	41.0	33.0	41.0
62-63	37.372605221462294	39.0	35.5	40.5	33.0	41.0
64-65	37.091685769762734	38.5	35.0	40.0	32.0	41.0
66-67	36.81197972002332	38.0	35.0	40.0	32.0	41.0
68-69	36.52658929707414	37.5	35.0	40.0	32.0	41.0
70-71	36.2253950662911	37.0	35.0	39.5	31.0	41.0
72-73	35.91856853257839	36.5	35.0	39.0	31.0	40.5
74-75	35.60379853495256	36.0	34.0	39.0	31.0	40.0
76-77	34.56024488757657	35.0	33.5	37.0	29.5	39.0
78-79	34.83760215626789	35.0	34.0	37.0	30.5	39.0
80-81	34.63472900835615	35.0	34.0	37.0	30.5	39.0
82-83	34.3923225051455	35.0	34.0	36.0	30.0	38.5
84-85	34.13870242939104	35.0	34.0	36.0	30.0	37.0
86-87	33.89908180913723	35.0	34.0	35.5	30.0	37.0
88-89	33.70581307215939	35.0	33.5	35.0	30.0	36.5
90-91	33.53406505425991	35.0	33.0	35.0	30.0	36.0
92-93	33.41001478069353	35.0	33.0	35.0	30.0	36.0
94-95	33.33492121677678	35.0	33.0	35.0	30.0	36.0
96-97	33.282227477268506	34.0	33.0	35.0	30.0	35.0
98-99	33.302201602728545	34.0	33.0	35.0	30.0	35.0
100-101	32.28073079724696	33.0	31.0	34.5	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	3.0
19	7.0
20	39.0
21	231.0
22	1086.0
23	3666.0
24	9445.0
25	19533.0
26	34759.0
27	54844.0
28	79977.0
29	108641.0
30	141545.0
31	179165.0
32	229393.0
33	303625.0
34	426220.0
35	623431.0
36	1073336.0
37	2176986.0
38	2878967.0
39	1277927.0
40	51631.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.72512314904321	21.682986619609412	15.367520406315352	24.22436982503202
2	13.590034712581847	22.111951849342724	41.643138698791915	22.654874739283517
3	16.541569206416444	27.497439907996903	31.76841863062702	24.192572254959632
4	11.750964421052261	19.334759563249907	39.843765908515586	29.07051010718224
5	12.54882961894705	38.4098185985858	36.15351602198615	12.887835760481003
6	28.408925525114796	39.34136451703331	19.338017558816745	12.911692399035148
7	25.119675979714	33.495516440407606	24.20208433425094	17.182723245627454
8	23.900310260339186	36.95017063491735	21.70958243026791	17.439936674475547
9	25.372535120058938	16.8454002121256	21.888391255447203	35.89367341236826
10-11	23.490217590506628	27.241141285758985	29.662011004855366	19.606630118879025
12-13	24.516060744694947	24.964892090078845	29.603715267563306	20.9153318976629
14-15	21.714984908624825	26.244526792359217	27.409916894070953	24.63057140494501
16-17	21.04387330686248	29.191121195245586	28.08012063059722	21.684884867294716
18-19	21.032951423713566	28.419095115765202	29.905024087666764	20.642929372854464
20-21	22.013005380843627	27.554037524343677	29.68733986122818	20.745617233584515
22-23	21.750117086564767	27.56258309838057	29.4356321617475	21.25166765330716
24-25	21.411757042254433	27.674867605125552	29.647151212821576	21.26622413979844
26-27	21.430325741886318	27.858154847314715	29.456519350031535	21.255000060767433
28-29	21.292525031950987	27.9705227417192	29.51184437390033	21.225107852429485
30-31	21.59630707220864	27.76463987136526	29.426646521761363	21.212406534664744
32-33	21.465318305239403	27.89852110483963	29.35949881544881	21.276661774472156
34-35	21.513359923795235	27.939606641094052	29.3735455842598	21.17348785085091
36-37	21.521298949618856	27.865790945623246	29.332174613677775	21.280735491080122
38-39	21.416940631831554	27.9692770058116	29.333618007961952	21.28016435439489
40-41	21.62390523466892	27.9477331036033	29.152763510277595	21.275598151450183
42-43	21.545323453205135	28.017007658047277	28.960830218766816	21.476838669980776
44-45	21.68965643172781	28.18613761205765	28.66562512755184	21.458580828662697
46-47	21.731377446553466	28.10134162117304	28.40100018170329	21.766280750570196
48-49	21.75425213605368	28.219414211893394	28.326177884079694	21.700155767973232
50-51	21.670491330657338	28.32653844960492	28.239110197250195	21.763860022487545
52-53	21.795720717726745	28.308353231036826	28.05310049038761	21.842825560848816
54-55	21.80565939063274	28.252695844938042	28.065386547063596	21.876258217365617
56-57	21.865982190721294	28.30833647749131	28.001138785598766	21.82454254618863
58-59	21.776773663483986	28.416080240068847	28.084149358078882	21.722996738368284
60-61	21.806332641166357	28.26062375597635	28.10598486388298	21.827058738974312
62-63	21.966718263529057	28.33514386936993	27.965091580150396	21.733046286950618
64-65	21.709762402734878	28.296195559952736	27.982871337519256	22.011170699793134
66-67	21.78233780678596	28.274714462471135	27.88686010259419	22.056087628148717
68-69	21.755338129028495	28.38144309157663	28.00612627002338	21.857092509371498
70-71	21.72267828195484	28.21255991740218	28.03730447540304	22.027457325239936
72-73	21.808247144122372	28.22251972881087	27.8415201583244	22.127712968742358
74-75	21.719730958423636	28.337033484236574	27.792786741117105	22.15044881622269
76-77	21.854659495486032	28.08834248499758	27.882570497079286	22.174427522437103
78-79	21.795932856445905	28.06139395368627	27.90105895960681	22.241614230261014
80-81	22.02010457071562	28.011791976603774	27.733317739886292	22.234785712794316
82-83	22.043990173655363	28.156933937550566	27.86822812894178	21.93084775985229
84-85	22.02973900720108	27.96752918151773	27.738560177416144	22.264171633865043
86-87	21.804287196736425	28.18697490205916	27.78285839131519	22.22587950988923
88-89	22.092799701903537	27.868339407136872	27.81051054304825	22.228350347911345
90-91	22.13846405233135	28.10770304315274	27.83715965267798	21.91667325183793
92-93	21.998789091274094	28.07720701843187	27.793875580045874	22.13012831024816
94-95	21.97278268666678	27.87993913786092	27.9306852002433	22.216592975229005
96-97	21.89419050923676	28.144516643724142	28.02058202224339	21.9407108247957
98-99	22.260129655430365	28.581221805421077	28.07505847513656	21.083590064011997
100-101	22.635950186276567	31.227976330217743	25.34623028373806	20.78984319976763
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2327.0
1	2105.0
2	2984.0
3	4332.5
4	5871.0
5	8950.5
6	11348.5
7	13351.0
8	14728.0
9	14439.0
10	13169.0
11	12143.5
12	13799.0
13	19292.5
14	31824.0
15	48530.5
16	61481.0
17	68040.0
18	68558.0
19	64531.0
20	58712.0
21	53216.0
22	50438.0
23	53543.5
24	62893.0
25	78957.5
26	99561.5
27	120531.5
28	142935.5
29	167421.0
30	188969.5
31	210263.0
32	234181.0
33	256936.0
34	278995.0
35	298983.5
36	319884.0
37	337123.0
38	346157.0
39	353558.5
40	363820.0
41	370767.5
42	372353.5
43	376461.0
44	380939.0
45	385768.0
46	389592.0
47	389921.0
48	387364.5
49	379100.0
50	366815.5
51	352542.5
52	334222.0
53	314513.0
54	294444.0
55	270047.5
56	244550.0
57	218092.0
58	190671.0
59	164165.5
60	135768.5
61	108165.0
62	83566.0
63	62666.5
64	45897.5
65	33631.0
66	25536.5
67	19635.5
68	14794.5
69	10685.0
70	7738.5
71	5758.5
72	4246.0
73	3147.0
74	2247.0
75	1544.5
76	1084.5
77	758.0
78	522.5
79	358.5
80	244.5
81	159.0
82	112.0
83	85.5
84	67.5
85	50.5
86	35.5
87	22.0
88	14.0
89	10.0
90	7.5
91	6.0
92	5.0
93	3.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.067299274026514E-5
2	0.0016124934337406807
3	1.033649637013257E-5
4	1.033649637013257E-5
5	2.067299274026514E-5
6	2.067299274026514E-5
7	2.067299274026514E-5
8	2.067299274026514E-5
9	1.033649637013257E-5
10-11	1.033649637013257E-5
12-13	0.0
14-15	5.995167894676891E-4
16-17	0.0030544346773741745
18-19	5.168248185066285E-5
20-21	0.0
22-23	0.001095776613325605
24-25	0.0053764149599780185
26-27	0.009949353961660547
28-29	0.3600692293974225
30-31	0.3293529782226789
32-33	0.33252691810436097
34-35	0.18838347038617675
36-37	0.047624713495335744
38-39	0.03809277813993647
40-41	0.022875056830797533
42-43	0.030324341619466778
44-45	0.01885263537666153
46-47	0.013791276006493342
48-49	0.0084203652171904
50-51	0.004250877281699785
52-53	0.0031035171470156653
54-55	3.081196243629627E-4
56-57	0.0025973213486150688
58-59	0.004970346446242144
60-61	0.003501912662298202
62-63	0.003012499622716163
64-65	2.3866720780090173E-4
66-67	5.8762216224036354E-5
68-69	8.070498654155337E-4
70-71	0.001150774885057368
72-73	0.001973205324361665
74-75	0.0021698007860057504
76-77	0.0010125195573709015
78-79	7.486453262820926E-5
80-81	0.0033494510684938146
82-83	0.0015373761008824923
84-85	7.740663518065773E-5
86-87	1.3051381723680323E-5
88-89	0.0014732830686147564
90-91	0.0037969325472589194
92-93	0.01570985047714934
94-95	0.001670567533900613
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	129.0
20-21	566.0
22-23	1256.0
24-25	2713.0
26-27	4145.0
28-29	7385.0
30-31	18256.0
32-33	24984.0
34-35	35147.0
36-37	49087.0
38-39	57787.0
40-41	63305.0
42-43	75992.0
44-45	90736.0
46-47	90039.0
48-49	78341.0
50-51	69009.0
52-53	63768.0
54-55	68483.0
56-57	72895.0
58-59	43392.0
60-61	74898.0
62-63	73925.0
64-65	78237.0
66-67	83382.0
68-69	83652.0
70-71	82326.0
72-73	80047.0
74-75	81739.0
76-77	82619.0
78-79	87279.0
80-81	89241.0
82-83	86710.0
84-85	88224.0
86-87	91996.0
88-89	99918.0
90-91	107616.0
92-93	111311.0
94-95	126659.0
96-97	186810.0
98-99	841679.0
100-101	6118775.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.33287157753205
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.6312948053302	71.2730484657477
2	8.024632282875691	13.053327738400927
3	2.2873667252818044	5.581143123541951
4	0.9180210825102579	2.98661163237095
5	0.4489154191086851	1.8255790065770328
6	0.24154408854517337	1.1787284600373968
7	0.12895965545233845	0.7342061366911476
8	0.08546356161654285	0.5560797505213422
9	0.05685196322411947	0.4161540081454094
>10	0.17386328958858302	2.104047625203957
>50	0.002535585112816329	0.1324610906537426
>100	4.133100772361471E-4	0.06484884268103672
>500	9.215472795139529E-5	0.04590805898218696
>1k	4.607654865598558E-5	0.04785606044524474
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	6.201897822079542E-5	0.0	0.0	0.0
3	0.0	6.201897822079542E-5	0.0	0.0	0.0
4	0.0	1.1370146007145827E-4	0.0	0.0	0.0
5	0.0	1.1370146007145827E-4	0.0	0.0	0.0
6	0.0	1.1370146007145827E-4	0.0	0.0	1.033649637013257E-5
7	0.0	1.2403795644159085E-4	0.0	0.0	1.033649637013257E-5
8	0.0	1.343744528117234E-4	0.0	0.0	1.033649637013257E-5
9	0.0	2.480759128831817E-4	0.0	1.033649637013257E-5	1.033649637013257E-5
10-11	0.0	2.480759128831817E-4	0.0	1.033649637013257E-5	1.033649637013257E-5
12-13	0.0	2.5841240925331425E-4	0.0	1.033649637013257E-5	1.033649637013257E-5
14-15	0.0	3.411043802143748E-4	0.0	2.067299274026514E-5	1.5504744555198856E-5
16-17	0.0	3.5144087658450736E-4	0.0	4.134598548053028E-5	5.168248185066285E-5
18-19	0.0	4.082916066202365E-4	0.0	5.168248185066285E-5	5.168248185066285E-5
20-21	0.0	4.961518257663633E-4	0.0	7.235547459092799E-5	5.6850730035729134E-5
22-23	0.0	6.098532858378216E-4	0.0	2.945901465487783E-4	7.235547459092799E-5
24-25	0.0	7.132182495391472E-4	0.0	9.044434323865998E-4	8.269197096106056E-5
26-27	0.0	7.545642350196775E-4	0.0	0.0028063587644909923	8.269197096106056E-5
28-29	0.0	7.545642350196775E-4	0.0	0.014894891269361034	8.269197096106056E-5
30-31	0.0	9.044434323865998E-4	0.0	0.02156193142809654	8.786021914612685E-5
32-33	0.0	9.096116805716661E-4	0.0	0.024042690556928356	9.302846733119313E-5
34-35	0.0	9.923036515327266E-4	0.0	0.02731935990626038	1.033649637013257E-4
36-37	0.0	0.0010749956224937872	0.0	0.032291214660294144	1.033649637013257E-4
38-39	0.0	0.0010956686152340525	1.033649637013257E-5	0.04096870336302044	1.1370146007145827E-4
40-41	0.0	0.0011990335789353781	1.033649637013257E-5	0.062396260338305254	1.1370146007145827E-4
42-43	0.0	0.001214538323490577	1.033649637013257E-5	0.07131665670572966	1.1886970825652456E-4
44-45	0.0	0.0013127350390068364	1.033649637013257E-5	0.08399953775188232	1.2403795644159085E-4
46-47	0.0	0.0014057635063380294	1.033649637013257E-5	0.09839310894729193	1.343744528117234E-4
48-49	0.0	0.0014057635063380294	1.033649637013257E-5	0.11091577429970753	1.343744528117234E-4
50-51	0.0	0.0015711474482601505	1.033649637013257E-5	0.12320586848379517	1.343744528117234E-4
52-53	0.0	0.0015711474482601505	1.033649637013257E-5	0.14204413311836178	1.3954270099678968E-4
54-55	0.0	0.0015763156964452168	1.033649637013257E-5	0.18238231020280413	1.4471094918185599E-4
56-57	0.0	0.0015814839446302831	1.033649637013257E-5	0.22353190225230188	1.4471094918185599E-4
58-59	0.0	0.0015814839446302831	1.033649637013257E-5	0.25483598150924835	1.4471094918185599E-4
60-61	0.0	0.0016021569373705483	1.033649637013257E-5	0.5828750303117756	1.4471094918185599E-4
62-63	0.0	0.0016279981782958797	1.033649637013257E-5	0.5899606985735015	1.5504744555198854E-4
64-65	0.0	0.0018502328502537299	1.033649637013257E-5	0.5965967292431266	1.6021569373705485E-4
66-67	0.0	0.0018605693466238625	1.033649637013257E-5	0.6024575226849918	1.705521901071874E-4
68-69	0.0	0.00189157883573426	1.033649637013257E-5	0.6081787734258601	1.7572043829225368E-4
70-71	0.0	0.0019122518284745253	1.033649637013257E-5	0.6138173321957674	1.7572043829225368E-4
72-73	0.0	0.001922588324844658	1.033649637013257E-5	0.6178227245391938	2.067299274026514E-4
74-75	0.0	0.001994943799435586	1.033649637013257E-5	0.620954682939344	2.067299274026514E-4
76-77	0.0	0.002103477011321978	1.033649637013257E-5	0.6241383238213448	2.067299274026514E-4
78-79	0.0	0.002139654748617442	1.033649637013257E-5	0.6270118698122417	2.067299274026514E-4
80-81	0.0	0.002139654748617442	1.033649637013257E-5	0.6283297730994335	2.1706642377278398E-4
82-83	0.0	0.002144822996802508	1.033649637013257E-5	0.628753569450609	2.480759128831817E-4
84-85	0.0	0.0021499912449875745	1.033649637013257E-5	0.6290429913489728	2.480759128831817E-4
86-87	0.0	0.00221201022320837	1.033649637013257E-5	0.6291050103271936	2.480759128831817E-4
88-89	0.0	0.002346384676020093	1.033649637013257E-5	0.6291205150717487	2.480759128831817E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13145	0.0	51.188316	1
GTATCAA	24095	0.0	36.588036	1
ATCAACG	30045	0.0	28.885664	3
TCAACGC	30425	0.0	28.496716	4
CAACGCA	30870	0.0	28.099812	5
TATCAAC	31320	0.0	27.94253	2
AACGCAG	31780	0.0	27.36262	6
ACGCAGA	36065	0.0	24.111576	7
CGCAGAG	36280	0.0	23.921436	8
CGAGACC	7150	0.0	22.90384	88-89
GAGACCG	7320	0.0	22.606588	88-89
GACCGAG	7510	0.0	22.14786	90-91
ACGAGAC	7485	0.0	22.033148	86-87
ACCGAGG	7910	0.0	21.745607	92-93
AGACCGA	7635	0.0	21.594158	90-91
ATCTCCG	7545	0.0	20.812098	74-75
CACGAGA	7985	0.0	20.65349	86-87
GCCCACG	7925	0.0	20.602112	82-83
GCAGAGT	42735	0.0	20.238283	9
CCCACGA	8130	0.0	19.743586	84-85
>>END_MODULE