FastQCFastQC Report
Fri 27 May 2016
SRR2139039_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139039_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9983812
Sequences flagged as poor quality0
Sequence length19-101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA148400.052.6481481
GTATCAA273750.036.842731
ATCAACG342800.028.8586083
TCAACGC343800.028.7621974
CAACGCA350400.028.2449195
TATCAAC357550.027.9079692
AACGCAG361150.027.570416
CGCAGAG411750.024.1302098
ACGCAGA412000.024.094757
CTTATAC138200.021.3786281
GCAGAGT478350.020.6363329
GTGGTAT73000.020.4420781
GTACATG328850.020.3942411
TGGTATC68000.020.368652
TACATGG330700.019.8652112
GAGTACT292750.019.3881812-13
ACATGGG330450.019.3481983
AGAGTAC442900.019.06548310-11
CAGAGTA474250.018.50598310-11
TATACAC188850.018.0744343
Produced by FastQC (version 0.11.5)