##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139039_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9983812
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.652106429888704	34.0	31.0	34.0	31.0	34.0
2	32.76473435196897	34.0	31.0	34.0	31.0	34.0
3	32.83242623158369	34.0	31.0	34.0	31.0	34.0
4	36.29923500162062	37.0	37.0	37.0	35.0	37.0
5	36.24453024556151	37.0	37.0	37.0	35.0	37.0
6	36.28470998852943	37.0	37.0	37.0	35.0	37.0
7	36.23558877110266	37.0	37.0	37.0	35.0	37.0
8	36.21534890681034	37.0	37.0	37.0	35.0	37.0
9	37.94820555515268	39.0	38.0	39.0	35.0	39.0
10-11	37.902806262778185	39.0	38.0	39.0	35.0	39.0
12-13	37.87582067851438	39.0	38.0	39.0	35.0	39.0
14-15	39.2666672309134	41.0	39.0	41.0	36.0	41.0
16-17	39.23716031511812	41.0	39.0	41.0	36.0	41.0
18-19	39.25734118390851	41.0	39.0	41.0	36.0	41.0
20-21	39.257989221599225	41.0	39.0	41.0	36.0	41.0
22-23	39.12703519878666	40.0	39.0	41.0	36.0	41.0
24-25	39.07529712952787	40.0	39.0	41.0	35.0	41.0
26-27	39.02197804401085	40.0	38.5	41.0	35.0	41.0
28-29	38.82533379089388	40.0	38.5	41.0	35.0	41.0
30-31	38.7157270622329	40.0	38.0	41.0	35.0	41.0
32-33	38.59477141419566	40.0	38.0	41.0	35.0	41.0
34-35	38.312013431296734	40.0	38.0	41.0	34.0	41.0
36-37	38.55630789640817	40.0	38.0	41.0	34.5	41.0
38-39	38.646793912472184	40.0	38.0	41.0	35.0	41.0
40-41	38.649571590421054	40.0	38.0	41.0	35.0	41.0
42-43	38.59563249989414	40.0	38.0	41.0	34.5	41.0
44-45	38.54611515014373	40.0	38.0	41.0	34.0	41.0
46-47	38.4739736188713	40.0	38.0	41.0	34.0	41.0
48-49	38.40931420243198	40.0	38.0	41.0	34.0	41.0
50-51	38.33330284359124	40.0	38.0	41.0	34.0	41.0
52-53	38.23568389567833	40.0	37.5	41.0	34.0	41.0
54-55	38.10341460108086	40.0	37.0	41.0	33.0	41.0
56-57	37.96905527627565	40.0	37.0	41.0	33.0	41.0
58-59	37.81310262159695	40.0	36.5	41.0	33.0	41.0
60-61	37.62774234975761	39.0	36.0	41.0	33.0	41.0
62-63	37.40943751686899	39.0	36.0	40.5	33.0	41.0
64-65	37.14900172907206	39.0	35.0	40.0	32.0	41.0
66-67	36.883255793139895	38.0	35.0	40.0	32.0	41.0
68-69	36.609219556649236	38.0	35.0	40.0	32.0	41.0
70-71	36.31262730487663	37.0	35.0	40.0	31.0	41.0
72-73	36.01200790975956	37.0	35.0	39.0	31.0	41.0
74-75	35.698426170537026	36.0	34.5	39.0	31.0	40.0
76-77	34.64533078544	35.0	33.5	37.0	29.5	39.0
78-79	34.92347291840967	35.0	34.0	37.0	30.5	39.0
80-81	34.71502050075449	35.0	34.0	37.0	30.5	39.0
82-83	34.461862331533254	35.0	34.0	36.5	30.0	39.0
84-85	34.196318613437384	35.0	34.0	36.0	30.0	37.5
86-87	33.94414794135361	35.0	34.0	36.0	30.0	37.0
88-89	33.74017536036959	35.0	33.5	35.0	30.0	37.0
90-91	33.56096506452728	35.0	33.0	35.0	30.0	36.0
92-93	33.42854352120396	35.0	33.0	35.0	30.0	36.0
94-95	33.34706420937383	35.0	33.0	35.0	30.0	36.0
96-97	33.290020933648336	34.0	33.0	35.0	30.0	35.0
98-99	33.3085121393163	34.0	33.0	35.0	30.0	35.0
100-101	32.28315113446889	33.0	31.0	34.5	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	9.0
20	41.0
21	253.0
22	1178.0
23	4096.0
24	10141.0
25	20911.0
26	36530.0
27	58049.0
28	84012.0
29	114212.0
30	148190.0
31	189170.0
32	240691.0
33	314625.0
34	442908.0
35	640020.0
36	1082261.0
37	2207714.0
38	3016345.0
39	1321832.0
40	50621.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.440491311384	21.866479643895502	15.596278815774859	24.09675022894564
2	13.286399907047977	22.140250379495665	42.2229356111921	22.350414102264253
3	16.471696028700862	27.674983030027313	31.851183881585897	24.00213705968593
4	11.449536800079327	19.706765252944518	39.709810182685764	29.133887764290385
5	12.301955635979086	38.517245568851415	36.264228158966546	12.916570636202948
6	27.972023909507097	39.552371333952536	19.449977568685945	13.025627187854418
7	24.875561045777008	33.55738605228004	24.00311864878051	17.563934253162444
8	23.617023045417447	37.310887520161394	21.771013802650632	17.301075631770527
9	24.944870756781075	17.069742499157638	21.764231938662306	36.22115480539898
10-11	23.145668207694616	27.494017315229897	29.644418384480797	19.715896092594694
12-13	24.33683647087906	25.14564076326758	29.619508059646954	20.898014706206407
14-15	21.50195707947131	26.501789807721625	27.377762913629482	24.618490199177582
16-17	20.795708407025618	29.725766035002287	27.999536035339844	21.47898952263225
18-19	20.772531961004454	28.597968690700853	30.000444720135317	20.62905462815938
20-21	21.681603649442057	27.666025929545025	29.91774421802818	20.73462620298474
22-23	21.504682074804407	27.82882636238665	29.660790369528243	21.0057011932807
24-25	21.230565366599034	27.962811552816813	29.863279977956463	20.94334310262769
26-27	21.22990901294299	28.09772678703383	29.71625233401668	20.956111866006495
28-29	20.914566474366573	28.04994418189682	29.823196126724326	21.21229321701228
30-31	21.368662561354093	27.96305923671158	29.80097787868729	20.867300323247036
32-33	21.35480703017719	27.911313681817557	29.84917559862341	20.884703689381837
34-35	21.073316031263353	28.068833167112352	29.977892323359097	20.879958478265195
36-37	21.21071097460462	28.116667878642282	29.66780085978023	21.00482028697287
38-39	20.98841269259585	28.0528987100557	29.80123538226001	21.157453215088438
40-41	21.141260465462448	27.958869213570935	29.80385982215535	21.096010498811264
42-43	21.210851345390385	28.011091689374982	29.605565838998388	21.172491126236245
44-45	21.208055790326593	28.29783747646777	29.26297330204777	21.23113343115787
46-47	21.45989926946122	28.160850314990153	28.754427499802222	21.624822915746403
48-49	21.38938414357848	28.25413574071859	28.78198377555698	21.574496340145952
50-51	21.270973974476664	28.43520324988823	28.74109223076265	21.552730544872457
52-53	21.361095228814772	28.469398005757206	28.762265040606334	21.407241724821684
54-55	21.357851342201723	28.46722154883205	28.555187370612018	21.61973973835421
56-57	21.57197868037535	28.62671709016176	28.28858736715562	21.512716862307276
58-59	21.320590099732026	28.783676375276922	28.621225864473903	21.27450766051715
60-61	21.461997751566795	28.80968008148369	28.33866550244518	21.389656664504336
62-63	21.446848236814358	28.95255209960219	28.292852855793306	21.30774680779015
64-65	21.31104381304305	28.81237400416184	28.305914171871976	21.570668010923136
66-67	21.372349886993835	28.720062030127796	28.280537557200624	21.627050525677745
68-69	21.422900170508115	28.73425594192987	28.363182576196444	21.479661311365568
70-71	21.406205679722976	28.525297032059644	28.438118665237518	21.63037862297987
72-73	21.413499246357315	28.5416241431699	28.272223469773976	21.772653140698807
74-75	21.40387419608818	28.619604737869768	28.172668789160717	21.803852276881333
76-77	21.496060304877318	28.412805805362296	28.270390017842388	21.820743871917998
78-79	21.4530185296671	28.353334855023892	28.284247926212764	21.90939868909624
80-81	21.61299039537315	28.379116814516376	28.14321959808519	21.864673192025286
82-83	21.67897326175178	28.539290394167704	28.231216008049493	21.550520336031024
84-85	21.6229450896282	28.309885151584336	28.141867029370864	21.925302729416593
86-87	21.44947643519234	28.515266675402962	28.20959955376657	21.825657335638134
88-89	21.7280196457435	28.250336142380476	28.20624193618011	21.815402275695906
90-91	21.759924773975218	28.41780383816036	28.25267986232756	21.569591525536865
92-93	21.606566798307735	28.34914078229776	28.24453807925029	21.799754340144215
94-95	21.624356913768334	28.20151928235419	28.336498198867783	21.83762560500969
96-97	21.60601222789134	28.442215326204025	28.403404825883094	21.54836762002154
98-99	21.861629678044753	28.850834948177667	28.509419479059932	20.778115894717647
100-101	22.205705773856927	31.6112072667647	25.817544356357896	20.365542603020476
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2837.0
1	2488.5
2	3198.5
3	4459.0
4	6030.0
5	9220.5
6	11726.0
7	14122.5
8	16152.0
9	16405.0
10	15027.5
11	13513.5
12	14998.0
13	20791.5
14	34409.0
15	52736.5
16	67220.5
17	74736.5
18	75075.0
19	70105.5
20	63172.0
21	56765.0
22	54132.0
23	57879.5
24	67785.5
25	83755.0
26	104660.0
27	127062.5
28	151977.5
29	180278.5
30	205355.5
31	228618.0
32	253985.5
33	277748.0
34	300396.0
35	320763.5
36	340514.5
37	358526.0
38	368327.0
39	373008.5
40	380083.5
41	388320.5
42	391997.0
43	394286.0
44	396056.0
45	395716.0
46	394219.5
47	391167.5
48	387588.0
49	386614.0
50	383746.5
51	365861.0
52	336229.5
53	311460.5
54	287294.0
55	259513.0
56	233189.5
57	205773.0
58	178144.0
59	151979.5
60	126217.5
61	101101.5
62	78411.5
63	59428.5
64	44126.5
65	32713.0
66	24709.5
67	18874.0
68	14276.5
69	10279.0
70	7239.0
71	5190.5
72	3703.5
73	2620.5
74	1827.5
75	1249.5
76	808.5
77	500.5
78	330.5
79	202.0
80	123.5
81	82.5
82	60.5
83	46.5
84	33.5
85	20.0
86	13.0
87	11.5
88	9.0
89	8.0
90	5.5
91	3.0
92	3.0
93	3.0
94	2.5
95	2.0
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.0016214247624054E-5
2	0.001672707779353217
3	1.0016214247624054E-5
4	2.0032428495248107E-5
5	1.0016214247624054E-5
6	1.0016214247624054E-5
7	1.0016214247624054E-5
8	5.0081071238120265E-5
9	0.0
10-11	0.0
12-13	0.0
14-15	5.809404263621952E-4
16-17	0.0029347507745538476
18-19	5.0081071238120265E-5
20-21	0.0
22-23	0.001011745463273239
24-25	0.005500561628382658
26-27	0.009842296739032605
28-29	0.3621742387246413
30-31	0.3319558594718689
32-33	0.33461708314195093
34-35	0.18851550456449348
36-37	0.04612156697517635
38-39	0.03640893522893161
40-41	0.022364041977665026
42-43	0.029879472390337183
44-45	0.018829512812197868
46-47	0.014351443778323907
48-49	0.008715511052205223
50-51	0.0044045048100662745
52-53	0.0032480571348307233
54-55	3.736529810034825E-4
56-57	0.0026256619424767143
58-59	0.005088193985743695
60-61	0.0037215894443318057
62-63	0.003073784978904614
64-65	4.005224392132453E-4
66-67	5.700037899551994E-5
68-69	8.757224782462182E-4
70-71	0.0010708042543053024
72-73	0.0019799840649472374
74-75	0.002075639382920714
76-77	0.00105376753026109
78-79	9.676878152016256E-5
80-81	0.0036680870314783004
82-83	0.001464939002906884
84-85	6.873179896048778E-5
86-87	2.5273749458036033E-5
88-89	0.0014772459990614693
90-91	0.004283448695916681
92-93	0.01518704722382749
94-95	0.0016759406500673315
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	146.0
20-21	622.0
22-23	1367.0
24-25	3339.0
26-27	4643.0
28-29	7253.0
30-31	18066.0
32-33	24146.0
34-35	34914.0
36-37	47912.0
38-39	56303.0
40-41	61924.0
42-43	75120.0
44-45	92208.0
46-47	92126.0
48-49	80437.0
50-51	69871.0
52-53	63142.0
54-55	70713.0
56-57	79712.0
58-59	50856.0
60-61	83003.0
62-63	81883.0
64-65	86674.0
66-67	96559.0
68-69	87983.0
70-71	82949.0
72-73	79041.0
74-75	79928.0
76-77	82054.0
78-79	87682.0
80-81	90266.0
82-83	87243.0
84-85	87726.0
86-87	92839.0
88-89	100755.0
90-91	109739.0
92-93	112972.0
94-95	128082.0
96-97	191126.0
98-99	865948.0
100-101	6334540.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.37554001920992
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.23259919421096	73.56440605115844
2	8.073417019206676	13.462510075532736
3	2.069966040579111	5.177536093641275
4	0.7537680462945394	2.513832716361282
5	0.36689218807266816	1.5294917154694119
6	0.18246317305722215	0.912777935235867
7	0.10493581737982809	0.6124356308980252
8	0.05834603392517927	0.3891705669192771
9	0.04239204616390964	0.31810137672917177
>10	0.11375459138414198	1.3858953919140806
>50	0.001172943256228317	0.06334057749615686
>100	2.53833106503899E-4	0.045270504720662574
>500	3.907336303257178E-5	0.025231363923622428
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	4.0064856990496215E-5	0.0	0.0	0.0
3	0.0	4.0064856990496215E-5	0.0	0.0	0.0
4	0.0	7.011349973336837E-5	0.0	4.0064856990496215E-5	0.0
5	0.0	7.011349973336837E-5	0.0	4.0064856990496215E-5	1.0016214247624054E-5
6	0.0	7.011349973336837E-5	0.0	5.008107123812027E-5	1.0016214247624054E-5
7	0.0	7.011349973336837E-5	0.0	6.009728548574432E-5	2.0032428495248107E-5
8	0.0	8.012971398099243E-5	0.0	6.009728548574432E-5	2.0032428495248107E-5
9	0.0	1.302107852191127E-4	0.0	9.014592822861649E-5	2.0032428495248107E-5
10-11	0.0	1.302107852191127E-4	0.0	1.0517024960005257E-4	2.0032428495248107E-5
12-13	0.0	1.302107852191127E-4	0.0	1.2019457097148864E-4	2.0032428495248107E-5
14-15	0.0	1.5525132083817284E-4	0.0	1.2019457097148864E-4	2.0032428495248107E-5
16-17	0.0	1.6025942796198486E-4	0.0	1.6025942796198486E-4	2.0032428495248107E-5
18-19	0.0	1.7528374933342092E-4	0.0	1.6526753508579688E-4	2.0032428495248107E-5
20-21	0.0	2.053323920762931E-4	0.0	1.8029185645723295E-4	2.0032428495248107E-5
22-23	0.0	2.403891419429773E-4	0.0	4.006485699049622E-4	2.0032428495248107E-5
24-25	0.0	2.7043778468584945E-4	0.0	0.001106791674362458	2.0032428495248107E-5
26-27	0.0	2.804539989334735E-4	0.0	0.0032452534162301936	2.0032428495248107E-5
28-29	0.0	2.804539989334735E-4	0.0	0.014463413373569134	2.0032428495248107E-5
30-31	0.0	3.105026416763457E-4	0.0	0.02050819867201025	2.0032428495248107E-5
32-33	0.0	3.205188559239697E-4	0.0	0.022721782020735166	2.0032428495248107E-5
34-35	0.0	3.6559182003827797E-4	0.0	0.025681573330908074	2.0032428495248107E-5
36-37	0.0	4.106647841525862E-4	0.0	0.030083699492738848	2.0032428495248107E-5
38-39	0.0	4.106647841525862E-4	0.0	0.03815676817632384	2.0032428495248107E-5
40-41	0.0	4.306972126478343E-4	0.0	0.057808580530162225	2.0032428495248107E-5
42-43	0.0	4.4071342689545835E-4	0.0	0.06592672217786152	2.0032428495248107E-5
44-45	0.0	5.208431408764508E-4	0.0	0.07746540099112442	2.0032428495248107E-5
46-47	0.0	5.909566406098192E-4	0.0	0.09040634979905471	2.0032428495248107E-5
48-49	0.0	5.909566406098192E-4	0.0	0.10257104200279413	2.0032428495248107E-5
50-51	0.0	6.109890691050673E-4	0.0	0.11377417763876163	2.0032428495248107E-5
52-53	0.0	6.109890691050673E-4	0.0	0.1302157933262365	2.0032428495248107E-5
54-55	0.0	6.109890691050673E-4	0.0	0.16625413218918786	2.0032428495248107E-5
56-57	0.0	6.210052833526913E-4	0.0	0.203950154510121	2.0032428495248107E-5
58-59	0.0	6.210052833526913E-4	0.0	0.2334378892551262	2.0032428495248107E-5
60-61	0.0	6.360296047241274E-4	0.0	0.5481022679513596	2.0032428495248107E-5
62-63	0.0	6.510539260955635E-4	0.0	0.554432515355858	2.0032428495248107E-5
64-65	0.0	7.962890326861123E-4	0.0	0.5605524222611564	2.0032428495248107E-5
66-67	0.0	8.163214611813604E-4	0.0	0.5662015670968163	2.0032428495248107E-5
68-69	0.0	8.263376754289845E-4	0.0	0.5717104849330096	2.0032428495248107E-5
70-71	0.0	8.363538896766085E-4	0.0	0.5770240865913742	2.0032428495248107E-5
72-73	0.0	8.513782110480446E-4	0.0	0.5807501182914903	2.0032428495248107E-5
74-75	0.0	8.664025324194806E-4	0.0	0.5840354365647109	2.0032428495248107E-5
76-77	0.0	9.26499817905225E-4	0.0	0.586910090053779	2.0032428495248107E-5
78-79	0.0	9.415241392766611E-4	0.0	0.5896845814003709	2.0032428495248107E-5
80-81	0.0	9.415241392766611E-4	0.0	0.5909015514314573	2.0032428495248107E-5
82-83	0.0	9.415241392766611E-4	0.0	0.5912120540731336	2.0032428495248107E-5
84-85	0.0	9.415241392766611E-4	0.0	0.5915025242863148	2.0032428495248107E-5
86-87	0.0	9.415241392766611E-4	0.0	0.5916427512857814	2.0032428495248107E-5
88-89	0.0	9.515403535242851E-4	0.0	0.5916778080356481	2.0032428495248107E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14840	0.0	52.648148	1
GTATCAA	27375	0.0	36.84273	1
ATCAACG	34280	0.0	28.858608	3
TCAACGC	34380	0.0	28.762197	4
CAACGCA	35040	0.0	28.244919	5
TATCAAC	35755	0.0	27.907969	2
AACGCAG	36115	0.0	27.57041	6
CGCAGAG	41175	0.0	24.130209	8
ACGCAGA	41200	0.0	24.09475	7
CTTATAC	13820	0.0	21.378628	1
GCAGAGT	47835	0.0	20.636332	9
GTGGTAT	7300	0.0	20.442078	1
GTACATG	32885	0.0	20.394241	1
TGGTATC	6800	0.0	20.36865	2
TACATGG	33070	0.0	19.865211	2
GAGTACT	29275	0.0	19.38818	12-13
ACATGGG	33045	0.0	19.348198	3
AGAGTAC	44290	0.0	19.065483	10-11
CAGAGTA	47425	0.0	18.505983	10-11
TATACAC	18885	0.0	18.074434	3
>>END_MODULE