FastQCFastQC Report
Fri 27 May 2016
SRR2139038_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139038_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9030552
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA116650.052.231251
GTATCAA221450.037.6464421
ATCAACG274200.029.9811063
TCAACGC274300.029.970184
CAACGCA280150.029.2830875
TATCAAC283750.029.1691112
AACGCAG288550.028.5347146
ACCGAGG59050.027.465292-93
CGAGACC57250.027.41850988-89
ACGAGAC59750.026.52584586-87
GACCGAG59900.026.50057290-91
GAGACCG59500.026.28539388-89
AGACCGA60050.025.99704490-91
GCCCACG58700.025.90731682-83
ATCTCCG58300.025.74190374-75
CCCACGA59750.025.6261684-85
TCTCCGA60000.024.82519776-77
CACGAGA62950.024.72943186-87
ACGCAGA332300.024.726247
CCACGAG63600.024.5118284-85
Produced by FastQC (version 0.11.5)