##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2139038_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9030552 Sequences flagged as poor quality 0 Sequence length 19-101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.68065008650634 34.0 31.0 34.0 31.0 34.0 2 32.78512587048942 34.0 31.0 34.0 31.0 34.0 3 32.85432750954759 34.0 31.0 34.0 31.0 34.0 4 36.315734852088774 37.0 37.0 37.0 35.0 37.0 5 36.26111703913559 37.0 37.0 37.0 35.0 37.0 6 36.30048871874056 37.0 37.0 37.0 35.0 37.0 7 36.25201881346788 37.0 37.0 37.0 35.0 37.0 8 36.23101566770226 37.0 37.0 37.0 35.0 37.0 9 37.96778956590915 39.0 38.0 39.0 35.0 39.0 10-11 37.924846177730885 39.0 38.0 39.0 35.0 39.0 12-13 37.90297929738958 39.0 38.0 39.0 35.0 39.0 14-15 39.30735225266407 41.0 39.0 41.0 36.0 41.0 16-17 39.282053024001186 41.0 39.0 41.0 36.0 41.0 18-19 39.296429000131994 41.0 39.0 41.0 36.0 41.0 20-21 39.29110430366654 41.0 39.0 41.0 36.0 41.0 22-23 39.15983278882533 40.0 39.0 41.0 36.0 41.0 24-25 39.11237899591265 40.0 39.0 41.0 36.0 41.0 26-27 39.06825259247594 40.0 39.0 41.0 35.5 41.0 28-29 38.88174181903365 40.0 39.0 41.0 35.0 41.0 30-31 38.771216016712685 40.0 38.0 41.0 35.0 41.0 32-33 38.658745422823536 40.0 38.0 41.0 35.0 41.0 34-35 38.38317217082102 40.0 38.0 41.0 34.0 41.0 36-37 38.63194644074119 40.0 38.0 41.0 35.0 41.0 38-39 38.73502002084879 40.0 38.0 41.0 35.0 41.0 40-41 38.72630246960469 40.0 38.0 41.0 35.0 41.0 42-43 38.67198153114353 40.0 38.0 41.0 35.0 41.0 44-45 38.622203479002735 40.0 38.0 41.0 35.0 41.0 46-47 38.55527790143931 40.0 38.0 41.0 34.0 41.0 48-49 38.49715996335824 40.0 38.0 41.0 34.0 41.0 50-51 38.43078093858972 40.0 38.0 41.0 34.0 41.0 52-53 38.32525430010135 40.0 38.0 41.0 34.0 41.0 54-55 38.189436040498556 40.0 37.0 41.0 34.0 41.0 56-57 38.054524694508274 40.0 37.0 41.0 33.5 41.0 58-59 37.89218750047286 40.0 37.0 41.0 33.0 41.0 60-61 37.70582127865761 39.0 36.0 41.0 33.0 41.0 62-63 37.47870009253185 39.0 36.0 40.5 33.0 41.0 64-65 37.202797973607495 39.0 35.0 40.0 32.0 41.0 66-67 36.92719011399717 38.0 35.0 40.0 32.0 41.0 68-69 36.64377287873242 37.5 35.0 40.0 32.0 41.0 70-71 36.339989968366226 37.0 35.0 39.5 31.5 41.0 72-73 36.03312022084984 37.0 35.0 39.0 31.0 41.0 74-75 35.71356531647791 36.0 34.5 39.0 31.0 40.0 76-77 34.6548380619956 35.0 33.5 37.0 30.0 39.0 78-79 34.9301810720776 35.0 34.0 37.0 31.0 39.0 80-81 34.71645864308397 35.0 34.0 37.0 31.0 39.0 82-83 34.46374323693479 35.0 34.0 36.5 30.5 38.5 84-85 34.202128711593254 35.0 34.0 36.0 30.0 37.0 86-87 33.95475320899541 35.0 34.0 36.0 30.0 37.0 88-89 33.75635193136308 35.0 34.0 35.0 30.0 37.0 90-91 33.57856754546424 35.0 33.0 35.0 30.0 36.0 92-93 33.448941094833565 35.0 33.0 35.0 30.0 36.0 94-95 33.36763401076668 35.0 33.0 35.0 30.0 36.0 96-97 33.311016406261956 34.0 33.0 35.0 30.0 35.0 98-99 33.32768763079995 34.0 33.0 35.0 30.0 35.0 100-101 32.311276405572684 33.0 31.0 34.5 27.5 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 2.0 19 8.0 20 20.0 21 197.0 22 893.0 23 3143.0 24 8369.0 25 17089.0 26 30694.0 27 48785.0 28 71042.0 29 97502.0 30 128501.0 31 164840.0 32 212165.0 33 278660.0 34 392711.0 35 569802.0 36 969468.0 37 1989087.0 38 2765919.0 39 1231869.0 40 49786.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.82637517617971 21.762756030860572 15.371795655459378 24.039073137500345 2 13.48365583079478 22.327731186423826 41.77939045272267 22.409222530058724 3 16.404855428549663 27.67639231798898 31.875437957723957 24.043314295737403 4 11.599999645647353 19.391118062328857 40.20841693841085 28.800465353612935 5 12.334074373305198 38.578671602799034 36.40233731005591 12.684916713839863 6 28.17055923048779 39.68669910765145 19.448678220334703 12.694063441526055 7 24.973334963355505 33.62084621183733 24.449457796156867 16.956361028650296 8 23.70618827545846 37.131147677133555 21.914469171019025 17.24819487638897 9 25.302772189341248 16.879865151100397 22.000128009893526 35.817234649664826 10-11 23.2100983417182 27.35067025803074 30.014139777944916 19.425091622306145 12-13 24.257143970822604 25.16933073415667 29.873716468273482 20.699808826747244 14-15 21.48308038485155 26.439896151872073 27.692031278462586 24.38499218481379 16-17 20.793061489900218 29.488546111888247 28.29470927641796 21.423683121793573 18-19 20.774945176364426 28.704015044047438 30.12957629317865 20.391463486409492 20-21 21.72565044535928 27.81164864685738 30.000785688748653 20.461915219034683 22-23 21.48231237998012 27.75677838408101 29.760434513851745 21.000474722087127 24-25 21.166969568891474 27.881445917289167 29.905059496429224 21.04652501739013 26-27 21.12776874879073 28.08181872783555 29.76669937196518 21.02371315140854 28-29 21.079468431463617 28.134116958184034 29.802409918438332 20.98400469191402 30-31 21.30289652309899 27.986555825411884 29.722273004299616 20.988274647189506 32-33 21.15725295945843 28.111598863658106 29.635449208307463 21.095698968576006 34-35 21.311064488693294 28.172280458045524 29.600355126694843 20.91629992656634 36-37 21.27871020963662 28.078386124937666 29.57167059436375 21.07123307106197 38-39 21.160018070549548 28.228873966921796 29.577130117805822 21.03397784472283 40-41 21.37429686647335 28.141389560768264 29.441702582839667 21.042610989918714 42-43 21.276775832836076 28.27004481550134 29.201887144870053 21.25129220679253 44-45 21.414676005356593 28.37860694484459 28.976519423621017 21.2301976261778 46-47 21.43934754461945 28.348241060717015 28.667381890936984 21.54502950372655 48-49 21.42557832746447 28.476252642099965 28.63852440935455 21.459644621081015 50-51 21.454414401390988 28.569041171380285 28.473730762385248 21.502813664843487 52-53 21.538632686745945 28.524602256992832 28.345453934329534 21.59131112193169 54-55 21.56775363929585 28.474474091597507 28.354465372841265 21.603306896265373 56-57 21.553217494499187 28.500474430616844 28.384474997663467 21.561833077220495 58-59 21.533375334600592 28.55717125786451 28.379711744544 21.529741662990897 60-61 21.548661235538034 28.439807050045562 28.398157102988698 21.613374611427705 62-63 21.6441701945314 28.50344073970507 28.36838049700245 21.484008568761077 64-65 21.483803928437435 28.40760772749936 28.39063963249395 21.717948711569253 66-67 21.550470877047303 28.4680694182302 28.228404636310465 21.753055068412024 68-69 21.507276926326114 28.630983786486365 28.28496754330876 21.576771743878766 70-71 21.512388347784373 28.43878006093819 28.32715585058282 21.721675740694614 72-73 21.56041553277123 28.457475745277595 28.12089345066736 21.861215271283815 74-75 21.445707607323687 28.538963654569628 28.09842564458715 21.916903093519537 76-77 21.59091178874946 28.31085984345188 28.189022374832856 21.90920599296581 78-79 21.56378699012006 28.26380511166075 28.188133183393525 21.984274714825666 80-81 21.761319050245326 28.28811406125177 27.987633263668442 21.962933624834463 82-83 21.78901313585303 28.415602970793426 28.116315062792452 21.679068830561096 84-85 21.68724061666491 28.229028017795134 28.014451528360258 22.069279837179703 86-87 21.54611327490961 28.44050122994495 28.069822760419726 21.943562734725713 88-89 21.86346754655607 28.106835762288885 28.09621290023699 21.93348379091806 90-91 21.876259524412177 28.400084920004424 28.08376864382538 21.63988691175802 92-93 21.699184636830626 28.29119361557805 28.14429481964819 21.86532692794314 94-95 21.758193490091628 28.113053774807838 28.18963658966553 21.939116145435005 96-97 21.714020440658686 28.35310962260744 28.260241292212168 21.672628644521705 98-99 21.99916263275277 28.744345265868798 28.377535196668568 20.87895690470986 100-101 22.305125198177578 31.53174639065449 25.715680648604316 20.447447762563613 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2604.0 1 2282.0 2 2974.0 3 4161.0 4 5463.5 5 8210.0 6 10406.5 7 12456.0 8 14203.0 9 14402.0 10 13185.0 11 11876.0 12 13032.5 13 17666.5 14 28857.5 15 44266.0 16 56262.0 17 62107.5 18 62223.5 19 58389.0 20 53262.5 21 48747.5 22 47617.5 23 51929.5 24 60971.5 25 75278.0 26 94372.5 27 114957.0 28 137857.0 29 163167.5 30 186509.0 31 208282.5 32 230364.5 33 252541.5 34 273506.5 35 292448.0 36 312454.0 37 328272.0 38 338682.5 39 348288.5 40 356471.0 41 363286.0 42 365553.0 43 366313.5 44 367536.0 45 368158.5 46 364867.0 47 358189.5 48 350557.0 49 338538.0 50 325624.5 51 310311.5 52 290839.5 53 271338.0 54 250959.5 55 228619.5 56 207066.5 57 185160.0 58 162267.0 59 138890.0 60 115633.5 61 93528.0 62 72546.5 63 54892.5 64 40778.5 65 30740.5 66 23595.5 67 17896.5 68 13619.0 69 9918.5 70 7145.0 71 5199.0 72 3724.0 73 2664.0 74 1805.5 75 1197.5 76 766.0 77 466.0 78 284.5 79 183.0 80 116.0 81 75.0 82 55.0 83 38.0 84 25.0 85 20.5 86 18.5 87 16.0 88 13.5 89 10.5 90 7.0 91 5.5 92 5.5 93 4.5 94 2.5 95 1.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0016056604291742075 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 7.751464140841003E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 6.311906514684817E-4 16-17 0.002834821171507567 18-19 3.8757320704205015E-5 20-21 0.0 22-23 0.0010742458365083111 24-25 0.005676844334725351 26-27 0.010216129872938823 28-29 0.3615239020925098 30-31 0.33155553459494624 32-33 0.334216021954841 34-35 0.1890569506608966 36-37 0.047131416457997385 38-39 0.03677704581248579 40-41 0.021902670869413787 42-43 0.029886305542089146 44-45 0.01826691298364367 46-47 0.013333960768330903 48-49 0.007982918078099881 50-51 0.004039014041242585 52-53 0.0027364672029944093 54-55 3.3552000508073153E-4 56-57 0.002028575382223449 58-59 0.004181286460726643 60-61 0.0028316269366371184 62-63 0.0023003932438992246 64-65 1.8659893705781495E-4 66-67 5.6496550948338556E-5 68-69 5.894728141793439E-4 70-71 8.833584674421914E-4 72-73 0.0015321293339542873 74-75 0.0018409815016611922 76-77 9.03468600608252E-4 78-79 1.0663638904127154E-4 80-81 0.0031406512349263062 82-83 0.0016223150940809833 84-85 2.0682066993351404E-5 86-87 1.3950488043873728E-5 88-89 0.0015609148062586892 90-91 0.003386661194547719 92-93 0.01474293630608283 94-95 0.0015649992927974892 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 18-19 147.0 20-21 569.0 22-23 1176.0 24-25 2893.0 26-27 3853.0 28-29 6509.0 30-31 16728.0 32-33 22217.0 34-35 31634.0 36-37 44123.0 38-39 52394.0 40-41 57211.0 42-43 69306.0 44-45 83294.0 46-47 82824.0 48-49 71889.0 50-51 64083.0 52-53 59055.0 54-55 62553.0 56-57 66108.0 58-59 39057.0 60-61 68795.0 62-63 67859.0 64-65 72252.0 66-67 76533.0 68-69 76952.0 70-71 77481.0 72-73 75068.0 74-75 76757.0 76-77 78626.0 78-79 82695.0 80-81 84105.0 82-83 82577.0 84-85 83587.0 86-87 87468.0 88-89 93876.0 90-91 101722.0 92-93 104509.0 94-95 118274.0 96-97 173760.0 98-99 786695.0 100-101 5723338.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.73570295804774 #Duplication Level Percentage of deduplicated Percentage of total 1 89.1921182246677 75.57756836084519 2 7.497773074291738 12.706581441400663 3 1.903861470043013 4.839751199965106 4 0.6646597681454558 2.252816507269529 5 0.3076306743319496 1.3033650720488006 6 0.15572005209881556 0.7917028847554173 7 0.08716402077674082 0.5170133201216908 8 0.05886215033380996 0.39901805489261377 9 0.0315511415683151 0.24061573439280554 >10 0.09922690314308076 1.2237790594449378 >50 9.492094427059384E-4 0.05105117340630493 >100 4.629206168470501E-4 0.08199900004662232 >500 2.039053987981993E-5 0.01473819141040283 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 4.429408080480573E-5 0.0 0.0 0.0 3 0.0 4.429408080480573E-5 0.0 2.2147040402402866E-5 0.0 4 0.0 5.536760100600716E-5 0.0 3.32205606036043E-5 0.0 5 0.0 5.536760100600716E-5 0.0 3.32205606036043E-5 0.0 6 3.32205606036043E-5 5.536760100600716E-5 0.0 3.32205606036043E-5 1.1073520201201433E-5 7 3.32205606036043E-5 5.536760100600716E-5 0.0 4.429408080480573E-5 1.1073520201201433E-5 8 3.32205606036043E-5 5.536760100600716E-5 0.0 4.429408080480573E-5 1.1073520201201433E-5 9 3.32205606036043E-5 9.966168181081289E-5 0.0 5.536760100600716E-5 1.1073520201201433E-5 10-11 3.8757320704205015E-5 9.966168181081289E-5 0.0 9.966168181081289E-5 1.1073520201201433E-5 12-13 4.9830840905406447E-5 9.966168181081289E-5 0.0 1.2734548231381647E-4 1.661028030180215E-5 14-15 5.536760100600716E-5 2.048601237222265E-4 0.0 1.384190025150179E-4 2.7683800503003584E-5 16-17 7.751464140841003E-5 2.3254392422523008E-4 0.0 1.6056604291742076E-4 3.8757320704205015E-5 18-19 7.751464140841003E-5 2.5469096462763294E-4 0.0 1.8271308331982363E-4 6.64411212072086E-5 20-21 7.751464140841003E-5 2.768380050300358E-4 0.0 2.8237476513063653E-4 8.858816160961146E-5 22-23 8.305140150901075E-5 3.100585656336401E-4 0.0 5.149186893558666E-4 9.412492171021218E-5 24-25 8.858816160961146E-5 3.2666884593544226E-4 0.0 0.0013620429847477763 1.0519844191141361E-4 26-27 8.858816160961146E-5 3.32205606036043E-4 0.0 0.0033718869012658364 1.2180872221321576E-4 28-29 8.858816160961146E-5 3.32205606036043E-4 0.0 0.014932641991320132 1.2180872221321576E-4 30-31 8.858816160961146E-5 3.8757320704205017E-4 0.0 0.02099539430147792 1.2180872221321576E-4 32-33 8.858816160961146E-5 3.8757320704205017E-4 0.0 0.02341495846544043 1.2180872221321576E-4 34-35 9.412492171021218E-5 4.595510883498595E-4 0.0 0.02690865408891948 1.2180872221321576E-4 36-37 9.966168181081289E-5 5.370657297582694E-4 0.0 0.03163151045473189 1.2180872221321576E-4 38-39 9.966168181081289E-5 5.536760100600716E-4 0.0 0.0401249004490534 1.328822424144172E-4 40-41 9.966168181081289E-5 5.979700908648773E-4 0.0 0.06043927325815742 1.4395576261561863E-4 42-43 9.966168181081289E-5 6.478009317702838E-4 0.0 0.06904339845449092 1.4949252271621936E-4 44-45 9.966168181081289E-5 7.197788130780932E-4 0.0 0.08135161615812633 1.5502928281682006E-4 46-47 9.966168181081289E-5 7.862199342853017E-4 0.0 0.09530425161164013 1.5502928281682006E-4 48-49 9.966168181081289E-5 7.862199342853017E-4 0.0 0.10750173411326351 1.661028030180215E-4 50-51 9.966168181081289E-5 9.191021766997189E-4 0.0 0.1198376356174019 1.7717632321922292E-4 52-53 9.966168181081289E-5 9.191021766997189E-4 0.0 0.13877335516145634 1.8824984342042436E-4 54-55 9.966168181081289E-5 9.301756969009203E-4 0.0 0.17926921853724997 1.9932336362162579E-4 56-57 9.966168181081289E-5 9.412492171021218E-4 0.0 0.21980937599384842 2.1039688382282722E-4 58-59 9.966168181081289E-5 9.412492171021218E-4 0.0 0.25185060669602477 2.2147040402402865E-4 60-61 1.1627196211261504E-4 9.633962575045247E-4 0.0 0.5886904809362705 2.2147040402402865E-4 62-63 1.328822424144172E-4 9.633962575045247E-4 0.0 0.5951131226529673 2.5469096462763294E-4 64-65 1.328822424144172E-4 0.0010519844191141361 0.0 0.6017793818140906 2.5469096462763294E-4 66-67 1.328822424144172E-4 0.0010796682196171398 0.0 0.607293994874289 2.6022772472823367E-4 68-69 1.328822424144172E-4 0.0011018152600195425 0.0 0.6129968577779077 2.713012449294351E-4 70-71 1.4949252271621936E-4 0.0011184255403213447 0.0 0.6183785885956916 2.8791152523123726E-4 72-73 2.1039688382282722E-4 0.0011294990605225461 0.0 0.6223816661484258 3.2113208583484153E-4 74-75 2.1039688382282722E-4 0.0011405725807237476 0.0 0.6260082440143193 3.432791262372444E-4 76-77 2.1039688382282722E-4 0.0012014769418303554 0.0 0.6294244249963901 3.764996868408487E-4 78-79 2.1039688382282722E-4 0.0012402342625345605 0.0 0.6324696430517205 3.764996868408487E-4 80-81 2.1039688382282722E-4 0.0012402342625345605 0.0 0.6336988037940539 3.8757320704205017E-4 82-83 2.1039688382282722E-4 0.0012623813029369634 0.0 0.6341583548824037 3.9864672724325157E-4 84-85 2.1039688382282722E-4 0.0012734548231381647 0.0 0.6344960972485403 3.9864672724325157E-4 86-87 2.1039688382282722E-4 0.001306675383741769 0.0 0.6345957589303511 4.041834873438523E-4 88-89 2.1039688382282722E-4 0.0013454327044459741 0.0 0.6346345162510553 4.0972024744445303E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11665 0.0 52.23125 1 GTATCAA 22145 0.0 37.646442 1 ATCAACG 27420 0.0 29.981106 3 TCAACGC 27430 0.0 29.97018 4 CAACGCA 28015 0.0 29.283087 5 TATCAAC 28375 0.0 29.169111 2 AACGCAG 28855 0.0 28.534714 6 ACCGAGG 5905 0.0 27.4652 92-93 CGAGACC 5725 0.0 27.418509 88-89 ACGAGAC 5975 0.0 26.525845 86-87 GACCGAG 5990 0.0 26.500572 90-91 GAGACCG 5950 0.0 26.285393 88-89 AGACCGA 6005 0.0 25.997044 90-91 GCCCACG 5870 0.0 25.907316 82-83 ATCTCCG 5830 0.0 25.741903 74-75 CCCACGA 5975 0.0 25.62616 84-85 TCTCCGA 6000 0.0 24.825197 76-77 CACGAGA 6295 0.0 24.729431 86-87 ACGCAGA 33230 0.0 24.72624 7 CCACGAG 6360 0.0 24.51182 84-85 >>END_MODULE