FastQCFastQC Report
Fri 27 May 2016
SRR2139037_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139037_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11138978
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA147500.050.752341
TATCTCG112550.037.34739794-95
GTATCAA275400.036.7621
TCAACGC324850.030.3785744
ATCAACG327400.030.089043
CAACGCA332000.029.6851925
TATCAAC341800.029.1769282
AACGCAG340150.029.0885586
TATGCCG45900.028.54070988-89
CCGTCTT51850.026.08013392-93
CTCGTAT50050.025.75687484-85
CGTATGC51450.025.27590486-87
CGCAGAG396400.024.9608298
ACGCAGA396300.024.9561967
TGCCGTC53300.024.81860490-91
GCCGTCT52950.024.38263790-91
GCTATCT178100.024.2775494-95
TCTCGTA51500.024.10326482-83
ATCTCGT86500.023.9048794-95
TCGTATG52800.023.73861384-85
Produced by FastQC (version 0.11.5)