##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139037_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11138978
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.699687799006334	34.0	31.0	34.0	31.0	34.0
2	32.80671889288227	34.0	31.0	34.0	31.0	34.0
3	32.87385243062694	34.0	31.0	34.0	31.0	34.0
4	36.32297900220289	37.0	37.0	37.0	35.0	37.0
5	36.265978620300714	37.0	37.0	37.0	35.0	37.0
6	36.302356104841934	37.0	37.0	37.0	35.0	37.0
7	36.25137772962654	37.0	37.0	37.0	35.0	37.0
8	36.22945641871274	37.0	37.0	37.0	35.0	37.0
9	37.96819573573087	39.0	38.0	39.0	35.0	39.0
10-11	37.9250213978338	39.0	38.0	39.0	35.0	39.0
12-13	37.903938359515564	39.0	38.0	39.0	35.0	39.0
14-15	39.3181469161713	41.0	39.0	41.0	36.0	41.0
16-17	39.292175143895605	41.0	39.0	41.0	36.0	41.0
18-19	39.30146459576453	41.0	39.0	41.0	36.0	41.0
20-21	39.29327867347253	41.0	39.0	41.0	36.0	41.0
22-23	39.162858253016005	40.5	39.0	41.0	36.0	41.0
24-25	39.11343696148238	40.0	39.0	41.0	36.0	41.0
26-27	39.06606858088948	40.0	39.0	41.0	35.5	41.0
28-29	38.875884161262995	40.0	39.0	41.0	35.0	41.0
30-31	38.768286116138874	40.0	38.0	41.0	35.0	41.0
32-33	38.66327389959497	40.0	38.0	41.0	35.0	41.0
34-35	38.38398886215488	40.0	38.0	41.0	34.0	41.0
36-37	38.63702399961874	40.0	38.0	41.0	35.0	41.0
38-39	38.74138297475503	40.0	38.0	41.0	35.0	41.0
40-41	38.73124332124634	40.0	38.0	41.0	35.0	41.0
42-43	38.67254969188074	40.0	38.0	41.0	35.0	41.0
44-45	38.621525195157105	40.0	38.0	41.0	34.5	41.0
46-47	38.550415070169734	40.0	38.0	41.0	34.0	41.0
48-49	38.48472857798035	40.0	38.0	41.0	34.0	41.0
50-51	38.41084633642552	40.0	38.0	41.0	34.0	41.0
52-53	38.299598551527794	40.0	38.0	41.0	34.0	41.0
54-55	38.15964802879999	40.0	37.0	41.0	34.0	41.0
56-57	38.01483645640019	40.0	37.0	41.0	33.0	41.0
58-59	37.84865783143932	40.0	36.5	41.0	33.0	41.0
60-61	37.653310514742834	39.0	36.0	41.0	33.0	41.0
62-63	37.41850784281345	39.0	36.0	40.5	33.0	41.0
64-65	37.14137970972559	39.0	35.0	40.0	32.0	41.0
66-67	36.85798409366808	38.0	35.0	40.0	32.0	41.0
68-69	36.57313067108993	37.5	35.0	40.0	32.0	41.0
70-71	36.2618094448099	37.0	35.0	39.5	31.0	41.0
72-73	35.95615194876788	36.5	35.0	39.0	31.0	40.5
74-75	35.6290336866074	36.0	34.0	39.0	31.0	40.0
76-77	34.567643273511806	35.0	33.5	37.0	29.5	39.0
78-79	34.81497582587366	35.0	34.0	37.0	30.5	39.0
80-81	34.597522487267554	35.0	34.0	37.0	30.0	39.0
82-83	34.352896922222556	35.0	34.0	36.0	30.0	38.5
84-85	34.11146329477759	35.0	34.0	36.0	30.0	37.0
86-87	33.881202956096104	35.0	34.0	35.5	30.0	37.0
88-89	33.68820275261369	35.0	33.0	35.0	30.0	36.5
90-91	33.53880338136606	35.0	33.0	35.0	30.0	36.0
92-93	33.427035163596585	35.0	33.0	35.0	30.0	36.0
94-95	33.35640392862139	35.0	33.0	35.0	30.0	35.5
96-97	33.293476929627445	34.0	33.0	35.0	30.0	35.0
98-99	33.29784734615748	34.0	33.0	35.0	30.0	35.0
100-101	32.25822904449828	33.0	31.0	34.5	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	1.0
17	0.0
18	2.0
19	11.0
20	55.0
21	301.0
22	1374.0
23	4536.0
24	11395.0
25	23720.0
26	42034.0
27	66221.0
28	95129.0
29	128000.0
30	163688.0
31	207403.0
32	261387.0
33	341246.0
34	480479.0
35	700661.0
36	1209734.0
37	2495509.0
38	3400001.0
39	1440756.0
40	65334.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.194706946132214	21.34622608038656	15.272983800306239	24.186083173174985
2	13.662001394051543	22.00245700686863	41.47188429300363	22.863657306076192
3	16.64577605697328	27.260270602971044	31.640017897515893	24.45393544253978
4	11.950210858719048	19.080804113484408	39.61013468474433	29.35885034305221
5	12.735928329498151	38.26768277443097	36.08922400048263	12.907164895588247
6	28.80842906924299	39.02686386971298	19.22931694978066	12.93539011126337
7	25.53135045806724	33.30694850226807	24.02400364270468	17.137697396960007
8	24.12075802340791	36.898393657582346	21.59107170591177	17.38977661309798
9	25.531552356059954	16.871269518621908	21.63009927840777	35.96707884691037
10-11	23.627953121013437	27.073327553030445	29.64032247841768	19.658396847538437
12-13	24.645640740110984	24.820499690366567	29.562537065788263	20.971322503734186
14-15	21.89262067435001	26.06767236704796	27.23648574401934	24.803221214582692
16-17	21.20528044123829	29.247182278107697	27.839947426315703	21.707589854338305
18-19	21.274912655724716	28.480679581867868	29.62065961771859	20.623748144688822
20-21	22.19438433367784	27.461302472030834	29.459601913904464	20.88471128038686
22-23	22.05812162386867	27.431375714372418	29.16040567626418	21.350096985494734
24-25	21.73607717447234	27.588999136096596	29.2860064488083	21.38891724062276
26-27	21.681327059940124	27.698532307557265	29.18004263869739	21.440097993805217
28-29	21.63128741670325	27.812159101154364	29.159664430265753	21.396889051876634
30-31	21.760877602098823	27.661389738558057	29.19175560905396	21.385977050289156
32-33	21.637601977592645	27.739813932858986	29.112623010976442	21.50996107857193
34-35	21.776633247493564	27.714243423329215	29.06413180263882	21.444991526538402
36-37	21.769970204867423	27.722552492077096	29.026734419318263	21.480742883737218
38-39	21.70864018494542	27.850399865026006	29.024290068064612	21.416669881963962
40-41	21.795973731522814	27.762242410406905	28.8677563885703	21.574027469499978
42-43	21.798101417771964	27.891657537314025	28.719704016485874	21.59053702842814
44-45	21.864775511062717	27.87621418642226	28.5431568725972	21.71585342991782
46-47	21.85927518474852	27.848996102139395	28.3118141882577	21.979914524854383
48-49	21.807725723980454	28.198185462540504	28.166697072248358	21.827391741230684
50-51	21.80508759717154	28.123844248978138	28.171615091990788	21.899453061859536
52-53	21.875835280918984	28.07458908267994	27.843880764460643	22.20569487194043
54-55	21.88451656368334	27.84123297692022	28.028363710463545	22.245886748932893
56-57	21.948411306053227	27.5686899469752	28.24250518087503	22.240393566096543
58-59	21.702185289172284	27.526216413627363	28.40566329305077	22.365935004149584
60-61	21.85679547738983	27.270186830868237	28.613260039765226	22.259757651976702
62-63	21.700346017548398	27.76119731210265	28.517808016337913	22.02064865401104
64-65	21.32333069054165	27.901579690029227	28.429998344516637	22.345091274912484
66-67	21.436220768738952	28.42519835931875	27.955999957193562	22.182580914748733
68-69	21.40709506917268	28.646359884571087	27.660826910728016	22.285718135528217
70-71	21.429707146468218	28.319686721253113	27.63747295467439	22.613133177604276
72-73	21.383065800613814	27.964985018499583	27.588377970761247	23.063571210125353
74-75	21.74551251377675	27.69677311008547	27.646780946961076	22.910933429176705
76-77	22.111970183955275	27.58036008683632	27.49979133442383	22.80787839478458
78-79	22.21373594924552	27.441746265205563	26.983725902917993	23.360791882630924
80-81	22.17212223691394	27.753758521595923	26.900892866090665	23.173226375399476
82-83	22.04257575101203	27.91170989573289	27.008369551762947	23.03734480149214
84-85	22.160222837949746	27.89399630194145	26.955115745480672	22.990665114628133
86-87	22.40128591856545	28.121768075845743	26.858229388027215	22.618716617561592
88-89	22.587282609686	27.81917179789415	27.138358274917714	22.455187317502144
90-91	22.30666679214151	27.92653105687746	27.33203124665816	22.434770904322864
92-93	22.178039323860375	27.426260089814885	27.28085720535795	23.11484338096679
94-95	21.967028059824727	27.466056540255853	27.867998084793378	22.698917315126042
96-97	21.60915531408352	27.673445704502203	28.107558065796518	22.60984091561776
98-99	21.941408977848635	27.855637523691527	28.33523348106899	21.867720017390845
100-101	22.578483989194297	30.74630519054863	25.543108824523465	21.13210199573361
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2688.0
1	2374.0
2	3120.5
3	4395.5
4	5830.0
5	8787.5
6	11183.0
7	13546.0
8	15667.0
9	16143.0
10	15030.5
11	13722.0
12	14945.5
13	19787.0
14	31453.5
15	47326.0
16	60014.0
17	66848.5
18	67565.0
19	63555.5
20	57616.0
21	52268.0
22	49986.0
23	54538.5
24	65123.5
25	80892.5
26	101457.0
27	123428.5
28	147400.5
29	174526.0
30	200151.5
31	226090.5
32	254523.5
33	282532.5
34	307283.0
35	326937.0
36	349672.0
37	371636.5
38	384948.0
39	393519.0
40	405823.5
41	419812.5
42	426139.5
43	433523.5
44	444760.5
45	450985.5
46	454191.5
47	453103.0
48	447324.5
49	441386.0
50	430593.5
51	413136.0
52	391582.0
53	368937.5
54	345503.0
55	318676.0
56	289775.5
57	258141.5
58	227460.5
59	197467.5
60	165009.5
61	134103.0
62	105156.5
63	79718.0
64	59312.5
65	44665.0
66	34532.5
67	26226.5
68	19667.5
69	14083.5
70	9922.5
71	7346.0
72	5278.0
73	3678.0
74	2505.0
75	1728.5
76	1145.5
77	671.5
78	392.5
79	256.0
80	187.5
81	133.0
82	93.0
83	62.0
84	41.0
85	32.0
86	25.0
87	17.5
88	12.0
89	8.0
90	5.0
91	3.5
92	4.0
93	4.0
94	2.5
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.7954968579702735E-5
2	0.0019930015123470037
3	1.7954968579702735E-5
4	2.69324528695541E-5
5	1.7954968579702735E-5
6	1.7954968579702735E-5
7	1.7954968579702735E-5
8	7.181987431881094E-5
9	0.0
10-11	0.0
12-13	0.0
14-15	6.643338374490011E-4
16-17	0.00292217113634662
18-19	9.875232718836503E-5
20-21	8.977710768144604E-6
22-23	0.001144742549869587
24-25	0.005895515833837872
26-27	0.010411745922676302
28-29	0.3656376876394447
30-31	0.334022139099299
32-33	0.33738216879070687
34-35	0.1916030973302506
36-37	0.0479476712651735
38-39	0.038573248961178724
40-41	0.023643555820308793
42-43	0.030848638903599224
44-45	0.01899445177740513
46-47	0.013751228484577507
48-49	0.009146920020071064
50-51	0.005149289225965728
52-53	0.003482921944447972
54-55	6.407060908700442E-4
56-57	0.0023249603888491426
58-59	0.0044481124065534165
60-61	0.0034348518576008285
62-63	0.0025704477382772067
64-65	2.8100740464371144E-4
66-67	7.963982490985394E-5
68-69	7.74266284655078E-4
70-71	0.0011023236219976857
72-73	0.0019296491985001905
74-75	0.002244982438326755
76-77	0.0010479015169579545
78-79	1.1124315497029649E-4
80-81	0.003558325809850035
82-83	0.0015452042174424387
84-85	6.582318280430785E-5
86-87	1.6655822800699924E-5
88-89	0.0015353796175487477
90-91	0.0037641036257728177
92-93	0.015816257236719235
94-95	0.0017652446973373227
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	170.0
20-21	621.0
22-23	1426.0
24-25	4051.0
26-27	4671.0
28-29	8003.0
30-31	21785.0
32-33	28402.0
34-35	41292.0
36-37	56907.0
38-39	44501.0
40-41	72082.0
42-43	85109.0
44-45	101176.0
46-47	101083.0
48-49	89400.0
50-51	81002.0
52-53	14701.0
54-55	13536.0
56-57	13065.0
58-59	13206.0
60-61	89145.0
62-63	88581.0
64-65	94703.0
66-67	100162.0
68-69	101842.0
70-71	101227.0
72-73	98411.0
74-75	100775.0
76-77	102194.0
78-79	108159.0
80-81	108944.0
82-83	106829.0
84-85	108124.0
86-87	113585.0
88-89	121378.0
90-91	129463.0
92-93	134785.0
94-95	151461.0
96-97	221100.0
98-99	1032118.0
100-101	7129803.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.6528162519938
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.09673527325288	72.8144327294071
2	8.02687477985937	13.268876125149593
3	2.126207548109773	5.27211125362558
4	0.7907741478818894	2.614388413668351
5	0.3700747993035682	1.5293862193165653
6	0.1917155977154336	0.9507500442368937
7	0.11039766805882428	0.6387274720900226
8	0.079902756438723	0.5283350276766069
9	0.05254159042528876	0.39084393771080184
>10	0.15320740615927525	1.8618925486202313
>50	0.0012639130547223744	0.06843809507395993
>100	2.6720435306601127E-4	0.0429429377587464
>500	3.731538726299372E-5	0.018875195665600912
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.977484289851367E-6
2	0.0	1.7954968579702735E-5	0.0	0.0	1.7954968579702735E-5
3	0.0	1.7954968579702735E-5	0.0	1.7954968579702735E-5	1.7954968579702735E-5
4	0.0	4.488742144925683E-5	0.0	3.590993715940547E-5	1.7954968579702735E-5
5	0.0	4.488742144925683E-5	0.0	3.590993715940547E-5	1.7954968579702735E-5
6	0.0	4.488742144925683E-5	0.0	3.590993715940547E-5	2.6932452869554102E-5
7	0.0	5.3864905739108204E-5	0.0	3.590993715940547E-5	2.6932452869554102E-5
8	0.0	5.3864905739108204E-5	0.0	7.181987431881094E-5	4.488742144925683E-5
9	0.0	1.7057220150717598E-4	0.0	9.875232718836503E-5	5.3864905739108204E-5
10-11	0.0	1.7057220150717598E-4	0.0	1.2568478005791915E-4	6.284239002895957E-5
12-13	0.0	1.7057220150717598E-4	0.0	1.4812849078254755E-4	6.284239002895957E-5
14-15	0.0	2.0648213866658144E-4	0.0	1.571059750723989E-4	6.733113217388526E-5
16-17	0.0	2.0648213866658144E-4	0.0	1.7954968579702733E-4	7.181987431881094E-5
18-19	0.0	2.244371072462842E-4	0.0	2.3790333368106122E-4	8.07973586086623E-5
20-21	0.0	2.513695601158383E-4	0.0	3.366556608694263E-4	8.977484289851367E-5
22-23	8.977484289851367E-6	2.827907551303181E-4	0.0	6.822888060287039E-4	1.0324106933329072E-4
24-25	1.7954968579702735E-5	3.007457237100208E-4	0.0	0.0015127061028399552	1.1670729576806777E-4
26-27	2.6932452869554102E-5	3.1421195014479786E-4	0.0	0.0036538361059695063	1.1670729576806777E-4
28-29	3.142119501447979E-5	3.1870069228972353E-4	0.0	0.017609335434543457	1.1670729576806777E-4
30-31	4.039867930433115E-5	3.6807685588390607E-4	0.0	0.025500544125322808	1.1670729576806777E-4
32-33	4.488742144925683E-5	3.6807685588390607E-4	0.0	0.02993991010665431	1.346622643477705E-4
34-35	4.488742144925683E-5	4.488742144925684E-4	0.0	0.03583811728508665	1.4363974863762188E-4
36-37	4.488742144925683E-5	5.38649057391082E-4	0.0	0.04341511402572121	1.4363974863762188E-4
38-39	4.488742144925683E-5	5.476265416809333E-4	0.0	0.0624114707830467	1.615947172173246E-4
40-41	4.488742144925683E-5	5.835364788403389E-4	0.0	0.2960280557157039	1.7057220150717598E-4
42-43	4.488742144925683E-5	5.835364788403389E-4	0.0	0.3149481038565657	1.84038427941953E-4
44-45	4.488742144925683E-5	6.104689317098929E-4	0.0	0.3470560764192191	2.109708808115071E-4
46-47	4.488742144925683E-5	6.374013845794471E-4	0.0	0.38503083496529034	2.2443710724628417E-4
48-49	4.488742144925683E-5	6.374013845794471E-4	0.0	0.41811286457339264	2.2443710724628417E-4
50-51	4.488742144925683E-5	7.047325167533323E-4	0.0	0.4493141112227711	2.3341459153613554E-4
52-53	4.488742144925683E-5	7.09221258898258E-4	0.0	0.6176778515946436	2.558583022607639E-4
54-55	4.488742144925683E-5	7.137100010431837E-4	0.0	1.2341751640051717	2.7830201298539235E-4
56-57	4.488742144925683E-5	7.316649696228865E-4	0.0	1.9107453125412404	2.827907551303181E-4
58-59	4.488742144925683E-5	7.451311960576635E-4	0.0	2.6145127497334135	2.8727949727524375E-4
60-61	4.488742144925683E-5	7.541086803475148E-4	0.0	3.1460920382462376	2.962569815650951E-4
62-63	4.488742144925683E-5	7.585974224924405E-4	0.0	3.156389212726697	3.1421195014479786E-4
64-65	4.488742144925683E-5	9.022371711300623E-4	0.0	3.162996641164028	3.1870069228972353E-4
66-67	4.488742144925683E-5	9.06725913274988E-4	0.0	3.1691955940661702	3.276781765795749E-4
68-69	4.488742144925683E-5	9.246808818546908E-4	0.0	3.174559640929356	3.4114440301435196E-4
70-71	4.488742144925683E-5	9.381471082894678E-4	0.0	3.1795017460309194	3.5909937159405467E-4
72-73	4.488742144925683E-5	9.516133347242449E-4	0.0	3.1835595689299323	4.3091924591286563E-4
74-75	4.488742144925683E-5	9.92012014028576E-4	0.0	3.187069765287264	4.3091924591286563E-4
76-77	4.488742144925683E-5	0.0010638318883473869	0.0	3.190395923216654	4.3091924591286563E-4
78-79	4.488742144925683E-5	0.001077298114782164	0.0	3.1929589949814066	4.3091924591286563E-4
80-81	4.488742144925683E-5	0.001077298114782164	0.0	3.1940632255490584	4.5336295663749406E-4
82-83	4.488742144925683E-5	0.001077298114782164	0.0	3.1944941447949713	5.027391202316766E-4
84-85	4.488742144925683E-5	0.001077298114782164	0.0	3.194763469323667	5.11716604521528E-4
86-87	4.488742144925683E-5	0.001104230567651718	0.0	3.194830800455841	5.162053466664536E-4
88-89	4.488742144925683E-5	0.0011850279262603804	0.0	3.194880176619435	5.296715731012306E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14750	0.0	50.75234	1
TATCTCG	11255	0.0	37.347397	94-95
GTATCAA	27540	0.0	36.762	1
TCAACGC	32485	0.0	30.378574	4
ATCAACG	32740	0.0	30.08904	3
CAACGCA	33200	0.0	29.685192	5
TATCAAC	34180	0.0	29.176928	2
AACGCAG	34015	0.0	29.088558	6
TATGCCG	4590	0.0	28.540709	88-89
CCGTCTT	5185	0.0	26.080133	92-93
CTCGTAT	5005	0.0	25.756874	84-85
CGTATGC	5145	0.0	25.275904	86-87
CGCAGAG	39640	0.0	24.960829	8
ACGCAGA	39630	0.0	24.956196	7
TGCCGTC	5330	0.0	24.818604	90-91
GCCGTCT	5295	0.0	24.382637	90-91
GCTATCT	17810	0.0	24.27754	94-95
TCTCGTA	5150	0.0	24.103264	82-83
ATCTCGT	8650	0.0	23.90487	94-95
TCGTATG	5280	0.0	23.738613	84-85
>>END_MODULE