FastQCFastQC Report
Fri 27 May 2016
SRR2139036_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139036_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8400129
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA115750.051.6394651
GTATCAA214450.038.667121
TATCTCG93200.032.02663494-95
ATCAACG275650.029.5178283
TCAACGC277250.029.3163264
CAACGCA279700.029.028655
TATCAAC285150.028.852822
AACGCAG287050.028.3605926
ACGCAGA326300.024.922687
CCGTCTT44450.024.64925892-93
TATGCCG42800.024.56692388-89
CGCAGAG335900.024.1975358
TGCCGTC46200.023.11435190-91
GCCGTCT45300.022.87084490-91
ATGCCGT44850.022.81039688-89
CTCGTAT46250.022.45149484-85
GCTATCT144500.022.28069394-95
TCGTATG47150.021.43877884-85
CGTATGC49500.021.35932586-87
ATCTCGT74950.021.18253594-95
Produced by FastQC (version 0.11.5)