##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139036_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8400129
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.71265726990621	34.0	31.0	34.0	31.0	34.0
2	32.81878361629923	34.0	31.0	34.0	31.0	34.0
3	32.884984742496215	34.0	31.0	34.0	31.0	34.0
4	36.32929339537524	37.0	37.0	37.0	35.0	37.0
5	36.27534458101774	37.0	37.0	37.0	35.0	37.0
6	36.31339578237429	37.0	37.0	37.0	35.0	37.0
7	36.264851527875344	37.0	37.0	37.0	35.0	37.0
8	36.24535313683873	37.0	37.0	37.0	35.0	37.0
9	37.98707817463279	39.0	38.0	39.0	35.0	39.0
10-11	37.94589368806122	39.0	38.0	39.0	35.0	39.0
12-13	37.92183387897971	39.0	38.0	39.0	35.0	39.0
14-15	39.336075493602536	41.0	39.0	41.0	36.0	41.0
16-17	39.31116611423467	41.0	39.0	41.0	36.0	41.0
18-19	39.32726056945077	41.0	39.0	41.0	36.0	41.0
20-21	39.32345963437129	41.0	39.0	41.0	36.0	41.0
22-23	39.19311253553035	40.5	39.0	41.0	36.0	41.0
24-25	39.14241392716313	40.5	39.0	41.0	36.0	41.0
26-27	39.0923070699174	40.0	39.0	41.0	35.5	41.0
28-29	38.90185557879418	40.0	39.0	41.0	35.0	41.0
30-31	38.78956591209466	40.0	38.0	41.0	35.0	41.0
32-33	38.67429299300756	40.0	38.0	41.0	35.0	41.0
34-35	38.3964931935042	40.0	38.0	41.0	34.0	41.0
36-37	38.64352228013695	40.0	38.0	41.0	35.0	41.0
38-39	38.746287919574115	40.0	38.0	41.0	35.0	41.0
40-41	38.73373906216352	40.0	38.0	41.0	35.0	41.0
42-43	38.67644478343568	40.0	38.0	41.0	35.0	41.0
44-45	38.62536131359844	40.0	38.0	41.0	35.0	41.0
46-47	38.55425116903456	40.0	38.0	41.0	34.0	41.0
48-49	38.4909209802479	40.0	38.0	41.0	34.0	41.0
50-51	38.41638007085786	40.0	38.0	41.0	34.0	41.0
52-53	38.30563820457309	40.0	38.0	41.0	34.0	41.0
54-55	38.167868046053755	40.0	37.0	41.0	34.0	41.0
56-57	38.029787343447765	40.0	37.0	41.0	33.5	41.0
58-59	37.863875885421294	40.0	36.5	41.0	33.0	41.0
60-61	37.66744278508521	39.0	36.0	41.0	33.0	41.0
62-63	37.4328145704759	39.0	35.5	40.5	33.0	41.0
64-65	37.157023969353105	39.0	35.0	40.0	32.5	41.0
66-67	36.87770735311782	38.0	35.0	40.0	32.0	41.0
68-69	36.59638288093595	37.5	35.0	40.0	32.0	41.0
70-71	36.28809710147373	37.0	35.0	39.5	31.5	41.0
72-73	35.98463082273882	36.5	35.0	39.0	31.0	40.5
74-75	35.65575403589354	36.0	34.5	39.0	31.0	40.0
76-77	34.593528954028855	35.0	33.5	37.0	29.5	39.0
78-79	34.841610512463234	35.0	34.0	37.0	31.0	39.0
80-81	34.619682235713526	35.0	34.0	37.0	30.5	39.0
82-83	34.37549418540311	35.0	34.0	36.0	30.0	38.5
84-85	34.133066752171544	35.0	34.0	36.0	30.0	37.0
86-87	33.903970462784564	35.0	34.0	35.5	30.0	37.0
88-89	33.712600828067856	35.0	33.5	35.0	30.0	36.5
90-91	33.56627081860133	35.0	33.0	35.0	30.0	36.0
92-93	33.45587904831446	35.0	33.0	35.0	30.0	36.0
94-95	33.38060142663924	35.0	33.0	35.0	30.0	35.5
96-97	33.32041375561903	34.5	33.0	35.0	30.0	35.0
98-99	33.32394800003701	34.0	33.0	35.0	30.0	35.0
100-101	32.27592774311745	33.0	31.0	34.5	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	2.0
20	44.0
21	230.0
22	1031.0
23	3462.0
24	8758.0
25	18126.0
26	31804.0
27	49236.0
28	70482.0
29	94740.0
30	121722.0
31	153551.0
32	193840.0
33	252522.0
34	358582.0
35	523659.0
36	909534.0
37	1888474.0
38	2594233.0
39	1078495.0
40	47600.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.27035397555847	21.381638470270932	15.243315339956725	24.10469221421388
2	13.655886930328123	21.962100188464532	41.67667362421546	22.705339256991884
3	16.576785089082584	27.419716392383116	31.64480727493763	24.358691243596674
4	11.708596786691439	19.16811495826194	39.77054156443111	29.35274669061551
5	12.579405049471276	38.39077671087592	36.06659756453682	12.963220675115982
6	28.614674516230526	39.149050960777146	19.306017635209564	12.930256887782768
7	25.32923609369239	33.44483958397296	24.035600890319873	17.19032343201478
8	24.08312641173545	36.80312024039651	21.597757746851773	17.51599560101627
9	25.46224295629781	16.70642399734861	21.74268058772438	36.0886524586292
10-11	23.55312183617191	27.09966282063423	29.606975655193846	19.74023968800001
12-13	24.582866525025985	24.942533620614636	29.531451243189245	20.943148611170137
14-15	21.807194255909454	26.101053960639526	27.391857681296116	24.699894102154904
16-17	21.043336675434222	29.1823741837111	27.98903344870764	21.785255692147032
18-19	21.082773688617102	28.349901150233432	29.849641451593378	20.71768370955609
20-21	22.02447648186288	27.443808854867317	29.741657638781415	20.790057024488387
22-23	21.79559058253049	27.45294920392794	29.449237145727515	21.302223067814058
24-25	21.465304921335306	27.604162883059352	29.61657950624803	21.313952689357315
26-27	21.421966872924077	27.710766722618313	29.549641469045838	21.317624935411768
28-29	21.373126243872377	27.792880393622614	29.54354571618638	21.29044764631863
30-31	21.629082598352895	27.647270446064386	29.47867968101829	21.24496727456443
32-33	21.44067452427035	27.80065184543864	29.39434309405758	21.364330536233428
34-35	21.59578154880025	27.85549246306879	29.316608790719144	21.232117197411817
36-37	21.54631535571674	27.808012312786172	29.31957504161512	21.326097289881965
38-39	21.467216934115147	27.926123504785956	29.307734303471566	21.298925257627328
40-41	21.56346122567993	27.740174113830967	29.238827458539614	21.457537201949496
42-43	21.610569432768756	27.842610019296448	28.997724681287444	21.549095866647345
44-45	21.59868782728359	27.979457150541997	28.76686957999457	21.65498544217984
46-47	21.697440494372692	27.950367415200784	28.43874327791655	21.913448812509976
48-49	21.67611856579617	28.18868333016109	28.377397678749837	21.757800425292906
50-51	21.68027621536324	28.285585993090546	28.21891906361354	21.815218727932674
52-53	21.7552047661899	28.172684549604472	27.99236944830912	22.079741235896506
54-55	21.769133968007644	27.952046516721836	28.098202708840038	22.18061680643049
56-57	21.807358512012982	27.73956761307881	28.297886228622193	22.155187646286016
58-59	21.591746120677104	27.6212431227475	28.53295414903596	22.254056607539432
60-61	21.764054132141865	27.432683080352078	28.643259021163747	22.160003766342314
62-63	21.538164489803595	27.882802054185547	28.674514792960615	21.904518663050247
64-65	21.20769610637075	27.986676089920316	28.52913888281412	22.276488920894813
66-67	21.28260524244525	28.536940132908388	28.047799782941524	22.132654841704834
68-69	21.29411710363617	28.75140773540017	27.75556485175826	22.198910309205402
70-71	21.300425652337303	28.410460555666628	27.733354634437607	22.555759157558462
72-73	21.252992692360962	28.09901284113481	27.69455737397553	22.953437092528695
74-75	21.596523458791584	27.83084819480249	27.71968871480384	22.852939631602094
76-77	21.932832407518532	27.66804223799181	27.598718575708013	22.800406778781646
78-79	22.06812258320894	27.550730850444026	27.097613583805373	23.28353298254166
80-81	22.03848382816727	27.885586669030708	26.997852044514413	23.07807745828761
82-83	21.994846159676452	27.931829921971236	27.15347498401369	22.91984893433862
84-85	22.050367497493035	27.96632615791855	27.044903489371535	22.93840285521688
86-87	22.24000222030586	28.255082437343717	26.98483570954531	22.520079632805107
88-89	22.44316984088303	27.889685578408052	27.270515294993565	22.396629285715353
90-91	22.203650280742735	27.96181859421669	27.492144500718542	22.34238662432203
92-93	22.07248829370676	27.448549190353017	27.43327334011081	23.04568917582942
94-95	21.858273522572556	27.48823561726604	28.003767165597825	22.64972369456358
96-97	21.509757762331276	27.742586906469953	28.198279481735167	22.54937584946361
98-99	21.8106882461076	27.939151803487373	28.445993873464953	21.804166076940078
100-101	22.442328627464533	30.84724786302433	25.758184442845156	20.95223906666598
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2195.0
1	2012.0
2	2771.5
3	3933.5
4	5377.0
5	8152.5
6	10284.5
7	12159.5
8	13684.5
9	13732.0
10	12525.5
11	11464.5
12	12823.5
13	17274.5
14	27728.0
15	42254.0
16	53562.0
17	58917.5
18	58849.5
19	54968.0
20	49294.0
21	43934.0
22	41123.0
23	43378.5
24	50190.5
25	61112.5
26	75470.0
27	91937.5
28	111289.5
29	132103.5
30	151856.5
31	171229.5
32	192306.0
33	213643.0
34	231888.5
35	248296.5
36	267170.0
37	286001.5
38	298559.0
39	305505.0
40	312866.5
41	320505.0
42	325621.5
43	331474.5
44	335299.5
45	338092.5
46	340446.5
47	339800.0
48	336916.0
49	328769.5
50	318704.5
51	307875.5
52	291954.5
53	273257.0
54	255447.0
55	236062.0
56	214467.5
57	190536.5
58	166660.5
59	143536.0
60	118738.0
61	95624.0
62	74493.0
63	56181.0
64	41981.5
65	31016.5
66	23429.0
67	17807.0
68	13386.0
69	9673.0
70	6739.0
71	4733.5
72	3269.5
73	2245.5
74	1550.5
75	1042.5
76	660.0
77	398.0
78	263.0
79	172.0
80	111.0
81	77.0
82	59.0
83	42.0
84	26.5
85	20.0
86	17.5
87	13.0
88	6.5
89	4.5
90	5.0
91	3.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.190457908444025E-5
2	0.001666641071821635
3	2.38091581688805E-5
4	2.38091581688805E-5
5	2.38091581688805E-5
6	2.38091581688805E-5
7	2.38091581688805E-5
8	8.333205359108176E-5
9	1.190457908444025E-5
10-11	5.952289542220125E-6
12-13	0.0
14-15	6.249904019331132E-4
16-17	0.0026547211358301757
18-19	9.5236632675522E-5
20-21	0.0
22-23	0.001101294509189648
24-25	0.00600180554316757
26-27	0.01083451819123943
28-29	0.3689973570514393
30-31	0.3381627295084745
32-33	0.34072728664114194
34-35	0.1941829546200036
36-37	0.050057082056336744
38-39	0.03978786508273532
40-41	0.02557391509326358
42-43	0.03304261185763505
44-45	0.021520380919394927
46-47	0.016119661579504584
48-49	0.01093721258297625
50-51	0.006554992733240597
52-53	0.005054530708577385
54-55	8.202674238418546E-4
56-57	0.003497873293037833
58-59	0.005964841757183248
60-61	0.00501723853512628
62-63	0.0038770952984169675
64-65	6.628962963065059E-4
66-67	5.301541403282224E-5
68-69	7.765370664237088E-4
70-71	0.0010010156250422728
72-73	0.0017765031898822948
74-75	0.002015393603955141
76-77	9.065480734423883E-4
78-79	1.1277347037941366E-4
80-81	0.003165262865456866
82-83	0.0013052821233896894
84-85	5.835663769186659E-5
86-87	3.69066662887424E-5
88-89	0.0015622910482442204
90-91	0.003842061709421918
92-93	0.015811773449586782
94-95	0.00169569588624178
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	134.0
20-21	550.0
22-23	1188.0
24-25	2964.0
26-27	3807.0
28-29	6435.0
30-31	16825.0
32-33	22609.0
34-35	32680.0
36-37	44313.0
38-39	35921.0
40-41	56441.0
42-43	66709.0
44-45	79957.0
46-47	80018.0
48-49	69517.0
50-51	62024.0
52-53	12764.0
54-55	11895.0
56-57	11609.0
58-59	11624.0
60-61	67409.0
62-63	67265.0
64-65	71572.0
66-67	75944.0
68-69	76256.0
70-71	75820.0
72-73	73524.0
74-75	73976.0
76-77	74680.0
78-79	79883.0
80-81	80614.0
82-83	79136.0
84-85	79659.0
86-87	83769.0
88-89	88984.0
90-91	96182.0
92-93	99453.0
94-95	111314.0
96-97	163863.0
98-99	769674.0
100-101	5381168.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.16473293011748
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.7949863414513	72.13651605348659
2	8.078204697308065	13.274870630182752
3	2.2531023108063875	5.553766488949121
4	0.8610172821624169	2.8298102014836237
5	0.4054438427036175	1.6656592526951646
6	0.21082923228792771	1.039363653887957
7	0.13310601256369278	0.7655633981582076
8	0.07103867798013858	0.4669499203157363
9	0.04837998758379626	0.3577615883086518
>10	0.14146383205297697	1.7243252661668147
>50	0.0020698091839996768	0.1167682048043242
>100	3.087905995269237E-4	0.04486328275975847
>500	4.918331614665629E-5	0.02378205880123848
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.38091581688805E-5	0.0	0.0	0.0
3	0.0	2.38091581688805E-5	0.0	0.0	0.0
4	0.0	8.333205359108176E-5	0.0	1.190457908444025E-5	0.0
5	0.0	8.333205359108176E-5	0.0	1.190457908444025E-5	0.0
6	0.0	8.333205359108176E-5	0.0	1.190457908444025E-5	0.0
7	0.0	8.333205359108176E-5	0.0	1.190457908444025E-5	0.0
8	0.0	9.5236632675522E-5	0.0	1.190457908444025E-5	0.0
9	0.0	1.90473265351044E-4	0.0	1.190457908444025E-5	0.0
10-11	0.0	1.90473265351044E-4	0.0	2.9761447711100627E-5	0.0
12-13	0.0	1.90473265351044E-4	0.0	4.7618316337761E-5	0.0
14-15	0.0	3.511850829909874E-4	0.0	6.547518496442138E-5	0.0
16-17	0.0	3.630896620754276E-4	0.0	8.333205359108176E-5	0.0
18-19	0.0	4.1666026795540874E-4	0.0	8.333205359108176E-5	0.0
20-21	0.0	4.6427858429316977E-4	0.0	1.0714121175996226E-4	0.0
22-23	0.0	5.476106378842515E-4	0.0	3.4523279344876725E-4	0.0
24-25	0.0	5.833243751375723E-4	0.0	0.0011487918816484842	0.0
26-27	0.0	6.071335333064528E-4	0.0	0.0033987573286076915	0.0
28-29	0.0	6.071335333064528E-4	0.0	0.017779488862611513	0.0
30-31	0.0	7.261793241508553E-4	0.0	0.025928173245910867	0.0
32-33	0.0	7.261793241508553E-4	0.0	0.030487627035251482	0.0
34-35	0.0	8.03559088199717E-4	0.0	0.03647563031472493	0.0
36-37	0.0	8.809388522485785E-4	0.0	0.04418384527189999	0.0
38-39	0.0	8.928434313330188E-4	0.0	0.06402877860566189	0.0
40-41	0.0	9.99984643092981E-4	0.0	0.3003287211422586	0.0
42-43	0.0	0.0010535552489729623	0.0	0.319834373972114	0.0
44-45	0.0	0.001184505618901805	0.0	0.3519886420791871	0.0
46-47	0.0	0.0012618853829506665	0.0	0.38984520356770713	0.0
48-49	0.0	0.0012678376724928866	0.0	0.4230232654760421	0.0
50-51	0.0	0.0014583109378439308	0.0	0.4551061061086086	0.0
52-53	0.0	0.0014642632273861509	0.0	0.6195202478438129	0.0
54-55	0.0	0.001470215516928371	0.0	1.2216240964870897	0.0
56-57	0.0	0.001476167806470591	0.0	1.8834948844237989	0.0
58-59	0.0	0.0014940246750972516	0.0	2.5743116564043245	0.0
60-61	0.0	0.0015118815437239119	0.0	3.096309592388403	0.0
62-63	0.0	0.0015297384123505722	0.0	3.106755860534999	0.0
64-65	0.0	0.001761877704497157	0.0	3.1142140793314006	0.0
66-67	0.0	0.0017916391522082578	0.0	3.1207794546964696	0.0
68-69	0.0	0.0018333051790037987	0.0	3.1269162652144984	0.0
70-71	0.0	0.0018749712057993394	0.0	3.1322197551966164	0.0
72-73	0.0	0.00189878036396822	0.0	3.136231598348073	0.0
74-75	0.0	0.001946398680305981	0.0	3.1394577392799565	0.0
76-77	5.952289542220125E-6	0.0020833013397770443	0.0	3.142671975632755	0.0
78-79	1.190457908444025E-5	0.0021428242351992453	0.0	3.1457552616156255	0.0
80-81	1.190457908444025E-5	0.0021487765247414654	0.0	3.1470766698939983	0.0
82-83	1.190457908444025E-5	0.0021547288142836855	0.0	3.147558805346918	0.0
84-85	1.190457908444025E-5	0.002196394841079226	0.0	3.147951656456704	0.0
86-87	1.190457908444025E-5	0.0022321085783325472	0.0	3.14804689308938	0.0
88-89	1.190457908444025E-5	0.0023452020796347296	0.0	3.1480826068266334	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11575	0.0	51.639465	1
GTATCAA	21445	0.0	38.66712	1
TATCTCG	9320	0.0	32.026634	94-95
ATCAACG	27565	0.0	29.517828	3
TCAACGC	27725	0.0	29.316326	4
CAACGCA	27970	0.0	29.02865	5
TATCAAC	28515	0.0	28.85282	2
AACGCAG	28705	0.0	28.360592	6
ACGCAGA	32630	0.0	24.92268	7
CCGTCTT	4445	0.0	24.649258	92-93
TATGCCG	4280	0.0	24.566923	88-89
CGCAGAG	33590	0.0	24.197535	8
TGCCGTC	4620	0.0	23.114351	90-91
GCCGTCT	4530	0.0	22.870844	90-91
ATGCCGT	4485	0.0	22.810396	88-89
CTCGTAT	4625	0.0	22.451494	84-85
GCTATCT	14450	0.0	22.280693	94-95
TCGTATG	4715	0.0	21.438778	84-85
CGTATGC	4950	0.0	21.359325	86-87
ATCTCGT	7495	0.0	21.182535	94-95
>>END_MODULE