FastQCFastQC Report
Fri 27 May 2016
SRR2139035_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139035_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8986017
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA140000.053.787811
GTATCAA256850.037.3563651
ATCAACG324400.029.006113
TCAACGC325600.028.9256844
CAACGCA329800.028.5181085
TATCAAC334150.028.3405062
AACGCAG335800.028.0984066
ACGCAGA395200.023.7987657
CGCAGAG395650.023.7716968
TACATGG312550.021.914092
GTACATG325500.021.4262681
TGGTATC59200.020.9695842
GCAGAGT448850.020.8979
GTGGTAT63250.020.8535441
ACATGGG322200.020.6690043
GAGTACT262550.020.44947412-13
AGAGTAC412600.019.30214910-11
TATCTCG162450.019.11658794-95
CAGAGTA442200.018.9069510-11
GTACTTT296200.018.07606714-15
Produced by FastQC (version 0.11.5)