FastQCFastQC Report
Fri 27 May 2016
SRR2139034_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139034_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8887996
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA128150.053.9651681
GTATCAA240450.038.3183561
TATCTCG84400.035.83981794-95
ATCAACG302250.029.9683723
TCAACGC303200.029.9172134
CAACGCA310600.029.2322545
TATCAAC313900.029.076962
AACGCAG318450.028.5794816
CCGTCTT37900.028.56138892-93
TATGCCG35700.028.52018488-89
TGCCGTC38950.027.01496590-91
ATGCCGT38350.026.10446488-89
CTCGTAT38800.026.01090484-85
GCCGTCT40200.025.16544590-91
ACGCAGA368150.024.6860717
CGCAGAG375750.024.1867688
TCTCGTA40050.024.10097182-83
CGTATGC42950.023.78812686-87
CTATCTC144450.023.77049694-95
TCGTATG41500.023.52129684-85
Produced by FastQC (version 0.11.5)