FastQCFastQC Report
Fri 27 May 2016
SRR2139033_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139033_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6632459
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82200.050.8250661
GTATCAA155250.038.3450781
TCAACGC195250.029.8547614
ATCAACG196200.029.7319563
CAACGCA201350.028.9714895
TATCAAC203050.028.9399642
AACGCAG206800.028.2492496
ACGCAGA236700.024.626717
CGCAGAG252000.023.1653848
GTACATG208600.021.3985541
TACATGG207950.020.8292772
GCAGAGT282600.020.445639
ACATGGG210850.019.87433
AGAGTAC253700.019.2764910-11
GAGTACT164100.019.03558512-13
CAGAGTA274950.018.08156410-11
CATGGGG150550.017.998944
GTGGTAT41800.017.9681531
TGGTATC41050.017.880612
AGTACTT180150.017.2449112-13
Produced by FastQC (version 0.11.5)