##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139033_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6632459
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.64490319502917	34.0	31.0	34.0	31.0	34.0
2	32.756323710406654	34.0	31.0	34.0	31.0	34.0
3	32.82564400322716	34.0	31.0	34.0	31.0	34.0
4	36.2916423606991	37.0	37.0	37.0	35.0	37.0
5	36.22992196408602	37.0	37.0	37.0	35.0	37.0
6	36.26943053850766	37.0	37.0	37.0	35.0	37.0
7	36.21767733505778	37.0	37.0	37.0	35.0	37.0
8	36.19774174254225	37.0	37.0	37.0	35.0	37.0
9	37.92701937546843	39.0	38.0	39.0	35.0	39.0
10-11	37.88244518360385	39.0	38.0	39.0	35.0	39.0
12-13	37.85824164159929	39.0	38.0	39.0	35.0	39.0
14-15	39.25568948108085	41.0	39.0	41.0	36.0	41.0
16-17	39.22699868932473	41.0	39.0	41.0	36.0	41.0
18-19	39.239153758809515	41.0	39.0	41.0	36.0	41.0
20-21	39.23262205551954	40.5	39.0	41.0	36.0	41.0
22-23	39.098847280266014	40.0	39.0	41.0	35.5	41.0
24-25	39.04931406115114	40.0	39.0	41.0	35.0	41.0
26-27	38.999906897655016	40.0	38.5	41.0	35.0	41.0
28-29	38.812080446132775	40.0	38.0	41.0	35.0	41.0
30-31	38.70469378317074	40.0	38.0	41.0	35.0	41.0
32-33	38.59251081122558	40.0	38.0	41.0	35.0	41.0
34-35	38.312017345831	40.0	38.0	41.0	34.0	41.0
36-37	38.56170396311329	40.0	38.0	41.0	34.5	41.0
38-39	38.66220254917313	40.0	38.0	41.0	35.0	41.0
40-41	38.64601971212082	40.0	38.0	41.0	35.0	41.0
42-43	38.587426206531404	40.0	38.0	41.0	34.5	41.0
44-45	38.53601960729401	40.0	38.0	41.0	34.0	41.0
46-47	38.46380872348102	40.0	38.0	41.0	34.0	41.0
48-49	38.39758210343892	40.0	38.0	41.0	34.0	41.0
50-51	38.32093055955728	40.0	38.0	41.0	34.0	41.0
52-53	38.20666506010476	40.0	37.5	41.0	33.5	41.0
54-55	38.061076119888156	40.0	37.0	41.0	33.0	41.0
56-57	37.92040244007535	40.0	37.0	41.0	33.0	41.0
58-59	37.74436315139755	39.5	36.0	41.0	33.0	41.0
60-61	37.54445931008738	39.0	36.0	41.0	33.0	41.0
62-63	37.305914755945565	39.0	35.5	40.5	32.5	41.0
64-65	37.01306571741867	38.5	35.0	40.0	32.0	41.0
66-67	36.724782891283276	38.0	35.0	40.0	32.0	41.0
68-69	36.43201062813338	37.0	35.0	40.0	31.0	41.0
70-71	36.12151794315923	37.0	35.0	39.5	31.0	41.0
72-73	35.817305473831574	36.0	34.5	39.0	31.0	40.5
74-75	35.5008116524038	36.0	34.0	39.0	31.0	40.0
76-77	34.45350859602755	35.0	33.5	37.0	29.5	39.0
78-79	34.73956682162955	35.0	34.0	37.0	30.0	39.0
80-81	34.539670563773676	35.0	34.0	37.0	30.0	39.0
82-83	34.309805222465364	35.0	34.0	36.0	30.0	38.5
84-85	34.065122713951226	35.0	34.0	36.0	30.0	37.0
86-87	33.8311454692667	35.0	34.0	35.5	30.0	37.0
88-89	33.64800243402891	35.0	33.0	35.0	30.0	36.5
90-91	33.49071330770519	35.0	33.0	35.0	30.0	36.0
92-93	33.37050582213732	35.0	33.0	35.0	30.0	36.0
94-95	33.30128550041778	34.5	33.0	35.0	30.0	35.5
96-97	33.26366563933844	34.0	33.0	35.0	30.0	35.0
98-99	33.28054252427375	34.0	33.0	35.0	30.0	35.0
100-101	32.2525204140357	33.0	31.0	34.5	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	5.0
20	29.0
21	179.0
22	870.0
23	3039.0
24	7554.0
25	15154.0
26	26416.0
27	41272.0
28	58732.0
29	78616.0
30	100511.0
31	126493.0
32	160353.0
33	208583.0
34	292743.0
35	426649.0
36	741918.0
37	1492148.0
38	1955139.0
39	860045.0
40	36010.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.07484388520155	21.375993428681582	15.284466892294397	24.264695793822472
2	13.680662512768901	22.18332183661084	41.29419170502049	22.84182394559977
3	16.741606203914145	27.270462926414307	31.475374589631777	24.512556280039767
4	11.928019457036976	19.143518263738983	39.276081465411245	29.652380813812794
5	12.822469011870258	38.13279509153392	35.936837302725884	13.107898593869935
6	28.925380465978	38.81902624652486	19.1696925680204	13.085900719476742
7	25.52718380920259	33.15440020058925	24.000992693660073	17.317423296548082
8	24.160299617562426	36.75455016279794	21.532780848117326	17.552369371522307
9	25.460180002620447	16.73058212647828	21.637389692118713	36.17184817878256
10-11	23.676128265549774	27.13470976601589	29.362352635726808	19.826809332707523
12-13	24.646139889152717	24.907038619239007	29.32439758198261	21.122423909625667
14-15	21.83407716752323	26.083110456983654	27.15581180880658	24.927000566686537
16-17	21.27432461324728	29.174616428693987	27.693676407217193	21.857382550841535
18-19	21.31688361502394	28.389039712902647	29.422872740617574	20.871203931455838
20-21	22.2682725502928	27.454321027303536	29.328070553575706	20.94933586882796
22-23	22.07685331223446	27.407063433164698	29.07975840341599	21.436324851184853
24-25	21.71600099517578	27.534340389483564	29.277474178107056	21.4721844372336
26-27	21.595862014956808	27.66334803694455	29.2105204191129	21.530269528985745
28-29	21.629210419151228	27.734613608598274	29.175074779177212	21.46110119307329
30-31	21.892637197119974	27.500660847068353	29.149947128444186	21.45675482736749
32-33	21.760769137208673	27.59776279116728	29.039142949761715	21.602325121862336
34-35	21.90990006199764	27.686324054330598	28.93889090036107	21.464884983310696
36-37	21.894890580661638	27.62603246318978	28.91147902790611	21.567597928242467
38-39	21.713614753390424	27.811634829225923	28.937132502569963	21.537617914813694
40-41	21.874803722539482	27.651213698797143	28.847929431935416	21.626053146727962
42-43	21.77744219443532	27.82390235612609	28.583432932117493	21.815222517321097
44-45	21.864294789068428	27.826622399685135	28.41401179590762	21.895071015338818
46-47	21.910118362458086	27.840786454236937	28.209512069776256	22.03958311352872
48-49	21.98703940761257	28.0408104529144	28.071093330951186	21.90105680852184
50-51	22.008857189800736	28.03693238155489	27.966376554579114	21.987833874065263
52-53	22.14769144371379	27.979538325283205	27.85241817762083	22.02035205338218
54-55	22.17231675712799	27.888909665791257	27.807061758278902	22.131711818801854
56-57	22.090005927224826	28.09590314078606	27.731307495997743	22.08278343599137
58-59	22.04575704221839	28.040023693009896	27.853055447097475	22.061163817674238
60-61	22.122900895745286	27.98556603232439	27.81367438050406	22.077858691426265
62-63	22.09016389976037	28.066833749630998	27.865023129684406	21.977979220924222
64-65	22.2029591088865	27.939557305268597	27.76215310503003	22.095330480814876
66-67	22.169840300000697	28.069583466494937	27.555433928165375	22.205142305338995
68-69	22.15222058497874	28.028226317497413	27.624096633886204	22.19545646363764
70-71	22.116670494560008	27.891021943724777	27.692531526462123	22.299776035253092
72-73	22.16994689714933	27.899535627791234	27.596107272202104	22.33441020285733
74-75	22.050529539822826	27.91680576249084	27.604111765342846	22.428552932343493
76-77	22.23428869161099	27.916140901114822	27.506973860748545	22.342596546525648
78-79	22.132846269610283	27.933437234063245	27.537029772439787	22.396686723886685
80-81	22.14059776368868	27.826510391029135	27.610037772480883	22.4228540728013
82-83	22.25890758940978	27.756537645188256	27.708976278204695	22.27557848719727
84-85	22.29049265808768	27.804897890060857	27.64730272593162	22.257306725919836
86-87	22.177552730599864	27.82873943968897	27.626437505953028	22.367270323758138
88-89	22.283992005585407	27.75976827037826	27.558504145284505	22.397735578751828
90-91	22.322976522113485	27.778263372436445	27.593591284292184	22.305168821157885
92-93	22.391570715108998	27.698691843054103	27.695689617692203	22.2140478241447
94-95	22.105801165135897	27.8048878065289	27.74604111593574	22.343269912399467
96-97	22.1348045009889	27.819381921460774	27.76030186392483	22.285511713625496
98-99	22.408439568891986	28.37543452619024	27.85499920852515	21.36112669639263
100-101	22.946704558177807	31.07376336684907	24.977698136844182	21.001833938128936
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1489.0
1	1376.5
2	1943.5
3	2739.0
4	3595.0
5	5404.0
6	6913.0
7	8400.5
8	9648.0
9	9791.5
10	9094.0
11	8387.5
12	9158.0
13	12239.0
14	19809.0
15	30206.5
16	38380.5
17	42386.0
18	42484.0
19	39953.5
20	36245.0
21	32833.5
22	31439.5
23	33755.5
24	39586.5
25	48861.5
26	61117.5
27	74632.0
28	90653.0
29	107873.0
30	122967.0
31	138512.0
32	154533.5
33	169079.0
34	182239.5
35	194290.0
36	209135.5
37	222495.5
38	232170.5
39	241071.5
40	248988.0
41	256220.5
42	259994.5
43	262350.0
44	265179.0
45	268671.5
46	272214.0
47	274887.0
48	273512.0
49	267109.5
50	258509.0
51	250263.0
52	236271.0
53	217863.0
54	203502.0
55	189598.0
56	174383.5
57	157558.5
58	139573.0
59	119569.5
60	99935.5
61	83363.5
62	66898.0
63	52928.5
64	42394.0
65	32319.0
66	24775.0
67	18567.5
68	12889.0
69	8968.5
70	6193.0
71	4431.0
72	3097.0
73	2201.5
74	1700.0
75	1224.5
76	754.5
77	403.0
78	313.0
79	261.0
80	166.5
81	86.0
82	52.0
83	28.0
84	15.0
85	9.5
86	7.0
87	5.0
88	5.0
89	6.5
90	6.5
91	6.0
92	4.5
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.002020366805132154
3	1.5077364217404134E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	6.0309456869616535E-5
9	0.0
10-11	0.0
12-13	7.538682108702067E-6
14-15	6.709427076744839E-4
16-17	0.0027893123802197645
18-19	7.538682108702067E-5
20-21	1.5077867763330249E-5
22-23	0.0011158513851824738
24-25	0.005444663479452164
26-27	0.010221846507545835
28-29	0.36281828111442993
30-31	0.3317715016054776
32-33	0.3350052933032105
34-35	0.1889219836269133
36-37	0.04676254933590013
38-39	0.03717983762478421
40-41	0.02225589964556419
42-43	0.02931738728478346
44-45	0.018061214431356963
46-47	0.0135564623093944
48-49	0.008381022871483845
50-51	0.004351129371887885
52-53	0.003231694904615875
54-55	4.5807133970325153E-4
56-57	0.0026300389361187454
58-59	0.004872061818316658
60-61	0.003849111153021791
62-63	0.0031295357122315785
64-65	3.8399030091698595E-4
66-67	5.169927325469931E-5
68-69	6.528427078209425E-4
70-71	8.972231734449385E-4
72-73	0.0016086127794420352
74-75	0.0019842646912129488
76-77	0.0010072093504597639
78-79	1.0091595911582573E-4
80-81	0.004084047090176781
82-83	0.0017938503429165641
84-85	5.702046949324103E-5
86-87	2.886315813171856E-5
88-89	0.0015207171390085588
90-91	0.004221858568924412
92-93	0.015591067091584317
94-95	0.0016431081360081228
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	139.0
20-21	428.0
22-23	869.0
24-25	2400.0
26-27	2981.0
28-29	4992.0
30-31	13076.0
32-33	17469.0
34-35	24468.0
36-37	34520.0
38-39	39261.0
40-41	43273.0
42-43	52144.0
44-45	62623.0
46-47	61702.0
48-49	54412.0
50-51	48785.0
52-53	44664.0
54-55	47087.0
56-57	50294.0
58-59	51689.0
60-61	51380.0
62-63	50711.0
64-65	55836.0
66-67	58335.0
68-69	59781.0
70-71	58880.0
72-73	57067.0
74-75	58337.0
76-77	59189.0
78-79	62823.0
80-81	63549.0
82-83	62364.0
84-85	63518.0
86-87	66983.0
88-89	70626.0
90-91	76279.0
92-93	78525.0
94-95	88977.0
96-97	131597.0
98-99	561264.0
100-101	4139162.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.87589283504441
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.20108432590935	80.14376712663459
2	6.467078805053552	11.366006480573459
3	1.3617369112841295	3.5899154065658565
4	0.4836263010525029	1.699963720139947
5	0.19991986171497833	0.8784068171831166
6	0.09813355653465652	0.5174144338536749
7	0.05725465605081289	0.3521912813599992
8	0.037671181834272184	0.2648310990270379
9	0.022607776379230417	0.17880106809059104
>10	0.06999747791915831	0.9289142976618506
>50	7.129491862770422E-4	0.041310723077235184
>100	1.7619708101718716E-4	0.03847754583257186
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	7.538682108702067E-5	0.0	0.0	0.0
3	0.0	7.538682108702067E-5	0.0	0.0	0.0
4	0.0	1.2061891373923307E-4	0.0	0.0	0.0
5	0.0	1.2061891373923307E-4	0.0	0.0	0.0
6	0.0	1.2061891373923307E-4	0.0	0.0	1.5077364217404134E-5
7	0.0	1.2061891373923307E-4	0.0	1.5077364217404134E-5	1.5077364217404134E-5
8	0.0	1.356962779566372E-4	0.0	3.0154728434808268E-5	1.5077364217404134E-5
9	0.0	2.1108309904365786E-4	0.0	6.0309456869616535E-5	3.0154728434808268E-5
10-11	0.0	2.1108309904365786E-4	0.0	1.1308023163053101E-4	3.0154728434808268E-5
12-13	1.5077364217404134E-5	2.1862178115235993E-4	0.0	1.8846705271755167E-4	3.0154728434808268E-5
14-15	2.26160463261062E-5	2.940086022393806E-4	0.0	2.1108309904365786E-4	6.78481389783186E-5
16-17	4.52320926522124E-5	3.166246485654868E-4	0.0	2.3369914536976405E-4	9.04641853044248E-5
18-19	6.0309456869616535E-5	3.3924069489159304E-4	0.0	2.789312380219764E-4	1.0554154952182893E-4
20-21	6.0309456869616535E-5	3.618567412176992E-4	0.0	3.5431805910899716E-4	1.0554154952182893E-4
22-23	6.0309456869616535E-5	3.844727875438054E-4	0.0	6.030945686961653E-4	1.3569627795663718E-4
24-25	7.538682108702067E-5	4.5985960863082604E-4	0.0	0.001243882547935841	1.6585100639144546E-4
26-27	9.04641853044248E-5	4.824756549569323E-4	0.0	0.0033471748562637176	1.6585100639144546E-4
28-29	9.04641853044248E-5	4.824756549569323E-4	0.0	0.01666802614234027	1.7338968850014755E-4
30-31	1.0554154952182893E-4	6.030945686961653E-4	0.0	0.023648845774998383	1.8846705271755167E-4
32-33	1.0554154952182893E-4	6.030945686961653E-4	0.0	0.027071407452349122	2.1108309904365786E-4
34-35	1.2061891373923307E-4	7.538682108702067E-4	0.0	0.031783083770287913	2.1108309904365786E-4
36-37	1.356962779566372E-4	9.347965814790562E-4	0.0	0.0390352959588593	2.1108309904365786E-4
38-39	1.356962779566372E-4	9.800286741312686E-4	0.0	0.05079564004843452	2.1108309904365786E-4
40-41	1.6585100639144546E-4	0.0010554154952182894	0.0	0.07816859478513172	2.1108309904365786E-4
42-43	1.6585100639144546E-4	0.0010780315415443956	0.0	0.09065265235714234	2.1862178115235993E-4
44-45	1.6585100639144546E-4	0.0012137278195010327	0.0	0.10879826019278822	2.26160463261062E-4
46-47	1.6585100639144546E-4	0.0013117306869141596	0.0	0.12887377064826183	2.26160463261062E-4
48-49	1.7338968850014755E-4	0.0013192693690228616	0.0	0.1470193784839077	2.26160463261062E-4
50-51	1.809283706088496E-4	0.0014926590575230093	0.0	0.16367232726203057	2.26160463261062E-4
52-53	1.9600573482625374E-4	0.0015077364217404134	0.0	0.18873844527346495	2.638538738045723E-4
54-55	1.9600573482625374E-4	0.0015303524680665196	0.0	0.24299434040979373	3.166246485654868E-4
56-57	1.9600573482625374E-4	0.0015529685143926258	0.0	0.2985393501867105	3.317020127828909E-4
58-59	1.9600573482625374E-4	0.0015529685143926258	0.0	0.3403639585257896	3.39240694891593E-4
60-61	2.1108309904365786E-4	0.001575584560718732	0.0	0.36679457799889903	3.5431805910899716E-4
62-63	2.1108309904365786E-4	0.0016132779712622423	0.0	0.38404308266360937	3.618567412176992E-4
64-65	2.1108309904365786E-4	0.0018243610703059	0.0	0.39771825200879496	3.618567412176992E-4
66-67	2.1108309904365786E-4	0.0018469771166320062	0.0	0.406040957056802	3.618567412176992E-4
68-69	2.26160463261062E-4	0.0018846705271755167	0.0	0.4144465876080048	3.618567412176992E-4
70-71	2.26160463261062E-4	0.001907286573501623	0.0	0.4219098828956198	3.618567412176992E-4
72-73	2.26160463261062E-4	0.001914825255610325	0.0	0.4265612497566891	4.070888338699116E-4
74-75	2.26160463261062E-4	0.0019751347124799413	0.0	0.43097137879027975	4.070888338699116E-4
76-77	2.26160463261062E-4	0.0020354441693495578	0.0	0.434356247057087	4.070888338699116E-4
78-79	2.26160463261062E-4	0.002050521533566962	0.0	0.43753003222485054	4.070888338699116E-4
80-81	2.26160463261062E-4	0.0020655988977843665	0.0	0.4391659262424389	4.221661980873157E-4
82-83	2.26160463261062E-4	0.0020655988977843665	0.0	0.4396634792616132	4.6739829073952816E-4
84-85	2.26160463261062E-4	0.0020655988977843665	0.0	0.4399348718175265	4.6739829073952816E-4
86-87	2.26160463261062E-4	0.0020957536262191747	0.0	0.44007810677759185	4.6739829073952816E-4
88-89	2.26160463261062E-4	0.002238988586284514	0.0	0.4401233388702441	4.824756549569323E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8220	0.0	50.825066	1
GTATCAA	15525	0.0	38.345078	1
TCAACGC	19525	0.0	29.854761	4
ATCAACG	19620	0.0	29.731956	3
CAACGCA	20135	0.0	28.971489	5
TATCAAC	20305	0.0	28.939964	2
AACGCAG	20680	0.0	28.249249	6
ACGCAGA	23670	0.0	24.62671	7
CGCAGAG	25200	0.0	23.165384	8
GTACATG	20860	0.0	21.398554	1
TACATGG	20795	0.0	20.829277	2
GCAGAGT	28260	0.0	20.44563	9
ACATGGG	21085	0.0	19.8743	3
AGAGTAC	25370	0.0	19.27649	10-11
GAGTACT	16410	0.0	19.035585	12-13
CAGAGTA	27495	0.0	18.081564	10-11
CATGGGG	15055	0.0	17.99894	4
GTGGTAT	4180	0.0	17.968153	1
TGGTATC	4105	0.0	17.88061	2
AGTACTT	18015	0.0	17.24491	12-13
>>END_MODULE