FastQCFastQC Report
Fri 27 May 2016
SRR2139032_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139032_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9234485
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA118950.048.59641
GTATCAA214150.036.1846121
ATCAACG262600.028.8532333
TCAACGC264700.028.5756174
CAACGCA270300.027.9994965
TATCAAC277350.027.567292
AACGCAG277650.027.3202046
ACGCAGA318750.023.8109877
CGCAGAG321400.023.5478028
GCAGAGT375900.019.9737599
GTACATG254900.019.558451
TACATGG250450.019.4083162
GTGGTAT60350.019.2279421
GAGTACT230550.018.87524812-13
AGAGTAC343700.018.71996510-11
ACATGGG255400.018.6615433
CAGAGTA367750.017.93983510-11
AGTACTT250650.017.30159612-13
TGGTATC61700.017.1354792
CATGGGG183550.016.8583474
Produced by FastQC (version 0.11.5)