##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2139032_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9234485 Sequences flagged as poor quality 0 Sequence length 19-101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.67121425829377 34.0 31.0 34.0 31.0 34.0 2 32.77883336212036 34.0 31.0 34.0 31.0 34.0 3 32.84736712442545 34.0 31.0 34.0 31.0 34.0 4 36.30423125924185 37.0 37.0 37.0 35.0 37.0 5 36.247143072948845 37.0 37.0 37.0 35.0 37.0 6 36.28380792215267 37.0 37.0 37.0 35.0 37.0 7 36.23418447265873 37.0 37.0 37.0 35.0 37.0 8 36.21317019844637 37.0 37.0 37.0 35.0 37.0 9 37.94644140956426 39.0 38.0 39.0 35.0 39.0 10-11 37.9016715604606 39.0 38.0 39.0 35.0 39.0 12-13 37.880642233974065 39.0 38.0 39.0 35.0 39.0 14-15 39.28936838383515 41.0 39.0 41.0 36.0 41.0 16-17 39.26078113722639 41.0 39.0 41.0 36.0 41.0 18-19 39.27083919677166 41.0 39.0 41.0 36.0 41.0 20-21 39.26534242606349 41.0 39.0 41.0 36.0 41.0 22-23 39.13461591847894 40.0 39.0 41.0 36.0 41.0 24-25 39.08931599470273 40.0 39.0 41.0 35.5 41.0 26-27 39.043481440555716 40.0 39.0 41.0 35.5 41.0 28-29 38.8555078578684 40.0 39.0 41.0 35.0 41.0 30-31 38.74701713874906 40.0 38.0 41.0 35.0 41.0 32-33 38.637844211210755 40.0 38.0 41.0 35.0 41.0 34-35 38.36156606176826 40.0 38.0 41.0 34.0 41.0 36-37 38.61075091136341 40.0 38.0 41.0 34.5 41.0 38-39 38.71373101947627 40.0 38.0 41.0 35.0 41.0 40-41 38.706275114036835 40.0 38.0 41.0 35.0 41.0 42-43 38.64762967988694 40.0 38.0 41.0 35.0 41.0 44-45 38.60009134434162 40.0 38.0 41.0 34.5 41.0 46-47 38.533205801654574 40.0 38.0 41.0 34.0 41.0 48-49 38.474351682673806 40.0 38.0 41.0 34.0 41.0 50-51 38.40499382889743 40.0 38.0 41.0 34.0 41.0 52-53 38.2994687396189 40.0 38.0 41.0 34.0 41.0 54-55 38.160229150649414 40.0 37.0 41.0 33.5 41.0 56-57 38.021894298018836 40.0 37.0 41.0 33.0 41.0 58-59 37.85992898196662 40.0 37.0 41.0 33.0 41.0 60-61 37.6697884922541 39.0 36.0 41.0 33.0 41.0 62-63 37.44368178301498 39.0 36.0 40.5 33.0 41.0 64-65 37.16553638058046 39.0 35.0 40.0 32.0 41.0 66-67 36.8867808331789 38.0 35.0 40.0 32.0 41.0 68-69 36.604274681888185 37.5 35.0 40.0 32.0 41.0 70-71 36.29835087635124 37.0 35.0 39.5 31.0 41.0 72-73 35.99380288418733 36.5 35.0 39.0 31.0 41.0 74-75 35.67483618750258 36.0 34.5 39.0 31.0 40.0 76-77 34.62004502210529 35.0 33.5 37.0 29.5 39.0 78-79 34.89455414255494 35.0 34.0 37.0 30.5 39.0 80-81 34.688441755309185 35.0 34.0 37.0 31.0 39.0 82-83 34.44262052397402 35.0 34.0 36.5 30.0 38.5 84-85 34.183991332438765 35.0 34.0 36.0 30.0 37.0 86-87 33.932560903063354 35.0 34.0 36.0 30.0 37.0 88-89 33.731681189055635 35.0 33.5 35.0 30.0 37.0 90-91 33.56276432735856 35.0 33.0 35.0 30.0 36.0 92-93 33.43329979239841 35.0 33.0 35.0 30.0 36.0 94-95 33.357609283933584 35.0 33.0 35.0 30.0 36.0 96-97 33.30682752295635 34.0 33.0 35.0 30.0 35.0 98-99 33.31841065211432 34.0 33.0 35.0 30.0 35.0 100-101 32.30462314403809 33.0 31.0 34.5 27.5 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 3.0 19 4.0 20 36.0 21 249.0 22 1120.0 23 3957.0 24 9653.0 25 20114.0 26 35095.0 27 55342.0 28 79193.0 29 105970.0 30 136250.0 31 171813.0 32 217765.0 33 282986.0 34 395581.0 35 574856.0 36 984049.0 37 2029464.0 38 2819931.0 39 1259970.0 40 51084.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.74187894614589 21.62754068039528 15.431753909395054 24.198826464063778 2 13.471395701842548 22.06569638140465 41.801315504353376 22.66159241239943 3 16.553505690896678 27.3060923267513 31.602596138279505 24.537805844072516 4 11.663833987493618 19.281172691276232 39.90708740119238 29.14790592003777 5 12.549362525360102 38.36225842588948 36.25888178929307 12.829497259457348 6 28.586412777756422 39.376619270051336 19.296138333648276 12.74082961854397 7 25.28231947964613 33.36692842102185 24.173692414899154 17.177059684432862 8 23.784262892983687 37.2531317410401 21.68934255096118 17.27326281501503 9 25.258853092511384 16.990541432467538 21.849599625750653 35.901005849270426 10-11 23.324608789770085 27.364216845877166 29.76127526332004 19.549899101032704 12-13 24.36973474969097 25.035001951922602 29.709599398342192 20.885663900044236 14-15 21.518892690367707 26.38498712100752 27.470377667547645 24.625742521077125 16-17 20.916028550357854 29.436551692023468 28.122459853089758 21.52495990452892 18-19 20.93470883038352 28.686563834671798 29.905165206920152 20.473562128024533 20-21 21.821670027623867 27.80346633331256 29.754339414208523 20.62052422485505 22-23 21.557609064822003 27.75112937714872 29.53723587217201 21.15402568585727 24-25 21.272160271904347 27.863446604242416 29.651103934352975 21.213289189500266 26-27 21.264508854852146 28.048222968890368 29.49471263189431 21.192555544363177 28-29 21.195242330863085 28.145220491018897 29.561872267707084 21.097664910410934 30-31 21.46538812664054 27.984792253399572 29.459397856174483 21.090421763785404 32-33 21.27471567419489 28.093706251430213 29.385054634662577 21.246523439712323 34-35 21.442729539173033 28.12909171601335 29.344794923364148 21.083383821449473 36-37 21.3903097742875 28.07880062720709 29.34441062509883 21.18647897340658 38-39 21.25917947627436 28.20396752369585 29.348989992623327 21.187863007406463 40-41 21.475329290394583 28.11430722059203 29.21574276179169 21.194620727221697 42-43 21.40122525147475 28.21310133192793 29.004086178875205 21.381587237722115 44-45 21.536768703312795 28.3508331918133 28.762621352373863 21.34977675250004 46-47 21.513982478608444 28.299340636665534 28.519162464930513 21.66751441979551 48-49 21.522601331846097 28.42475406270555 28.474919051689294 21.57772555375906 50-51 21.525896977795995 28.53975968990011 28.31477563649273 21.61956769581117 52-53 21.64720967688572 28.45981825515717 28.199564747843148 21.693407320113966 54-55 21.690114999170593 28.406910665480535 28.222063907243218 21.680910428105655 56-57 21.672538486084704 28.462904002345148 28.21865503547132 21.645902476098826 58-59 21.642080520266244 28.506094052840243 28.27840442789094 21.573420999002575 60-61 21.724442273432835 28.455847211301215 28.201147023128225 21.618563492137717 62-63 21.671278919149433 28.524390261591716 28.165229281368596 21.639101537890255 64-65 21.67105647694448 28.46831573387464 28.132413593738537 21.728214195442348 66-67 21.725989148331024 28.557224581687628 27.997837192009218 21.71894907797213 68-69 21.646273924594677 28.51977846770496 28.06258429474891 21.771363312951454 70-71 21.694595455800158 28.339615914005044 28.10705128948258 21.85873734071222 72-73 21.679313797073117 28.369086353687884 28.084676161809213 21.86692368742979 74-75 21.62528105415296 28.453689990052943 28.034803712954627 21.886225242839473 76-77 21.747124657812513 28.410315441240485 27.949570673605535 21.892989227341463 78-79 21.700098789929225 28.401078644014575 27.913460934006178 21.98536163205002 80-81 21.70484647521054 28.424217968246456 27.938428621963133 21.93250693457987 82-83 21.729172720551986 28.41995839919794 28.02430141908065 21.82656746116942 84-85 21.85921513781511 28.419957439228625 27.93379184333308 21.787035579623186 86-87 21.695785256690534 28.44034224905491 27.989464757957627 21.874407736296927 88-89 21.76644712351267 28.42514146424716 27.954990955889137 21.85342045635103 90-91 21.764723325915167 28.503457488468932 27.95561799575563 21.776201189860267 92-93 21.813254808873253 28.462074621442373 27.980154377481124 21.744516192203246 94-95 21.650999573809116 28.513498617841783 28.05206350710934 21.78343830123976 96-97 21.60905137018439 28.546087787676335 28.101753674813317 21.743107167325963 98-99 21.841409832252946 29.082152810870916 28.148360113123427 20.928077243752714 100-101 22.385869552736956 31.82140335051579 25.396416067894517 20.396311028852736 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3224.0 1 2667.0 2 3060.5 3 4221.0 4 5510.5 5 8168.5 6 10292.5 7 12072.0 8 13484.0 9 13486.5 10 12345.0 11 11223.5 12 12407.5 13 16897.5 14 27631.5 15 42249.0 16 53515.5 17 59250.0 18 60188.0 19 57355.0 20 52622.0 21 48376.0 22 47065.0 23 51309.0 24 60859.5 25 75409.5 26 94348.5 27 115572.0 28 138777.5 29 163007.5 30 185656.5 31 208095.5 32 231288.0 33 252442.0 34 272649.0 35 292815.5 36 314279.5 37 332471.5 38 343541.5 39 351198.5 40 359367.5 41 367205.5 42 369769.0 43 371262.5 44 373871.0 45 374861.0 46 373304.0 47 369084.0 48 362329.5 49 353112.5 50 341753.5 51 325098.0 52 305289.0 53 286389.0 54 266924.0 55 244166.5 56 221254.0 57 198939.0 58 175477.0 59 150296.0 60 123315.5 61 98205.0 62 75975.5 63 57571.0 64 42854.5 65 31964.0 66 24168.0 67 18327.5 68 13965.0 69 10072.5 70 6966.0 71 4893.5 72 3444.5 73 2403.0 74 1619.0 75 1062.0 76 665.5 77 407.5 78 260.5 79 176.5 80 123.5 81 82.0 82 64.5 83 47.0 84 27.5 85 14.0 86 9.5 87 10.5 88 9.0 89 7.0 90 5.5 91 3.0 92 2.0 93 1.5 94 1.0 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0016893199783203936 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 5.4144871100012614E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 7.25541272740169E-4 16-17 0.002977967910500694 18-19 5.4144871100012614E-5 20-21 0.0 22-23 0.001158821965416204 24-25 0.00567067144974534 26-27 0.010283405341551822 28-29 0.3619588595241065 30-31 0.3305434380294822 32-33 0.3337571891026633 34-35 0.1887107096722405 36-37 0.046771614365822244 38-39 0.037054089779109296 40-41 0.022667305086645138 42-43 0.02978826917447029 44-45 0.018339431114556305 46-47 0.013941575662468834 48-49 0.00841738888245019 50-51 0.004513908610782862 52-53 0.003279372728096799 54-55 5.269764823863087E-4 56-57 0.0027254709239151755 58-59 0.005238686073312825 60-61 0.0037048107507133563 62-63 0.0032575213236197553 64-65 2.7421005111458163E-4 66-67 9.222791090808401E-5 68-69 6.579361845349617E-4 70-71 0.0010464765947676672 72-73 0.0017206397186652846 74-75 0.0019477356742604306 76-77 8.576781183032185E-4 78-79 1.1725793873702646E-4 80-81 0.0033516122407200345 82-83 0.00143127611246768 84-85 9.42115292435615E-5 86-87 2.0415548972070304E-5 88-89 0.0015978374097127166 90-91 0.0036769677114790187 92-93 0.014670952603202796 94-95 0.0017234043699072056 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 18-19 166.0 20-21 529.0 22-23 1331.0 24-25 3070.0 26-27 4783.0 28-29 7675.0 30-31 17178.0 32-33 23411.0 34-35 33028.0 36-37 45708.0 38-39 53019.0 40-41 58309.0 42-43 69806.0 44-45 83412.0 46-47 82397.0 48-49 72315.0 50-51 64480.0 52-53 59081.0 54-55 62698.0 56-57 66502.0 58-59 68018.0 60-61 67993.0 62-63 66728.0 64-65 71846.0 66-67 76447.0 68-69 76224.0 70-71 75812.0 72-73 74269.0 74-75 75964.0 76-77 76685.0 78-79 81583.0 80-81 82865.0 82-83 80909.0 84-85 81643.0 86-87 86248.0 88-89 92095.0 90-91 97036.0 92-93 102205.0 94-95 118749.0 96-97 180252.0 98-99 782324.0 100-101 5909692.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.78467878452395 #Duplication Level Percentage of deduplicated Percentage of total 1 89.16769910049646 75.60054726190678 2 7.532385334192947 12.772617420816163 3 1.8916223136607981 4.811417707361064 4 0.6934540529338638 2.3517711651929565 5 0.3016832588740311 1.2789059099151556 6 0.15081392497212112 0.7672026110996751 7 0.08209153599547898 0.487207315721341 8 0.05424814245054391 0.36795290658611707 9 0.03148789914554036 0.2402722273188713 >10 0.09298042464190673 1.179523276531981 >50 0.001194377053202234 0.06318665287464631 >100 2.9983226973306486E-4 0.055148752377547135 >500 3.980331337220294E-5 0.024246792297662002 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 1.0828974220002523E-5 0.0 0.0 0.0 3 0.0 1.0828974220002523E-5 0.0 0.0 0.0 4 0.0 3.248692266000757E-5 0.0 0.0 0.0 5 1.0828974220002523E-5 4.3315896880010094E-5 0.0 0.0 0.0 6 1.0828974220002523E-5 4.3315896880010094E-5 0.0 0.0 1.0828974220002523E-5 7 1.0828974220002523E-5 6.497384532001513E-5 0.0 0.0 1.0828974220002523E-5 8 1.0828974220002523E-5 6.497384532001513E-5 0.0 0.0 1.0828974220002523E-5 9 1.0828974220002523E-5 9.746076798002271E-5 0.0 0.0 1.0828974220002523E-5 10-11 2.1657948440005047E-5 9.746076798002271E-5 0.0 1.0828974220002523E-5 1.0828974220002523E-5 12-13 2.1657948440005047E-5 9.746076798002271E-5 0.0 1.0828974220002523E-5 1.0828974220002523E-5 14-15 2.1657948440005047E-5 1.5160563908003533E-4 0.0 2.1657948440005047E-5 2.1657948440005047E-5 16-17 3.2486922660007574E-5 1.6243461330003785E-4 0.0 3.248692266000757E-5 2.1657948440005047E-5 18-19 4.3315896880010094E-5 2.0575051018004793E-4 0.0 5.9559358210013874E-5 2.1657948440005047E-5 20-21 4.3315896880010094E-5 2.49066407060058E-4 0.0 8.663179376002019E-5 2.1657948440005047E-5 22-23 5.4144871100012614E-5 2.8155332972006563E-4 0.0 1.0287525509002397E-4 2.1657948440005047E-5 24-25 9.204628087002145E-5 2.9238230394006815E-4 0.0 1.1911871642002775E-4 2.1657948440005047E-5 26-27 1.0828974220002523E-4 2.9238230394006815E-4 0.0 1.840925617400429E-4 2.1657948440005047E-5 28-29 1.0828974220002523E-4 2.9238230394006815E-4 0.0 5.360342238901249E-4 2.1657948440005047E-5 30-31 1.0828974220002523E-4 3.5735614926008327E-4 0.0 0.002046676127580477 2.7072435550006307E-5 32-33 1.0828974220002523E-4 3.73599610590087E-4 0.0 0.005095032370511187 3.248692266000757E-5 34-35 1.0828974220002523E-4 4.0608653325009464E-4 0.0 0.009307503342092169 4.3315896880010094E-5 36-37 1.0828974220002523E-4 4.223299945800984E-4 0.0 0.015382557879513585 4.3315896880010094E-5 38-39 1.0828974220002523E-4 4.494024301301047E-4 0.0 0.02627109145772612 4.3315896880010094E-5 40-41 1.0828974220002523E-4 4.98132814120116E-4 0.0 0.05196824728179211 4.3315896880010094E-5 42-43 1.0828974220002523E-4 5.089617883401185E-4 0.0 0.06340364405811477 4.8730383990011354E-5 44-45 1.0828974220002523E-4 5.739356336601337E-4 0.0 0.07938179551972849 5.4144871100012614E-5 46-47 1.0828974220002523E-4 6.172515305401438E-4 0.0 0.09792099938437282 5.9559358210013874E-5 48-49 1.0828974220002523E-4 6.22666017650145E-4 0.0 0.11389373635887654 6.497384532001513E-5 50-51 1.0828974220002523E-4 7.03883324300164E-4 0.0 0.1291950769317401 6.497384532001513E-5 52-53 1.0828974220002523E-4 7.03883324300164E-4 0.0 0.15246112804341552 7.038833243001641E-5 54-55 1.0828974220002523E-4 7.092978114101652E-4 0.0 0.20379046584622748 7.580281954001767E-5 56-57 1.0828974220002523E-4 7.147122985201665E-4 0.0 0.25533638313343954 7.580281954001767E-5 58-59 1.0828974220002523E-4 7.147122985201665E-4 0.0 0.29286960777996823 7.580281954001767E-5 60-61 1.0828974220002523E-4 7.201267856301678E-4 0.0 0.31734850400428394 7.580281954001767E-5 62-63 1.0828974220002523E-4 7.363702469601716E-4 0.0 0.33286642406154754 7.580281954001767E-5 64-65 1.0828974220002523E-4 8.013440922801867E-4 0.0 0.34477829570355034 8.121730665001893E-5 66-67 1.0828974220002523E-4 8.121730665001893E-4 0.0 0.35216365612159206 8.663179376002019E-5 68-69 1.0828974220002523E-4 8.446599891601969E-4 0.0 0.3592728776970237 8.663179376002019E-5 70-71 1.0828974220002523E-4 8.663179376002018E-4 0.0 0.3658568940227852 8.663179376002019E-5 72-73 1.0828974220002523E-4 8.771469118202044E-4 0.0 0.3702372140947763 1.0828974220002523E-4 74-75 1.0828974220002523E-4 9.150483215902132E-4 0.0 0.3738161900744871 1.0828974220002523E-4 76-77 1.1911871642002776E-4 0.0010179235766802372 0.0 0.37707571131470785 1.0828974220002523E-4 78-79 1.1911871642002776E-4 0.0010504104993402448 0.0 0.38002660678965855 1.0828974220002523E-4 80-81 1.1911871642002776E-4 0.0010504104993402448 0.0 0.38138022856715886 1.1370422931002649E-4 82-83 1.24533203530029E-4 0.0010504104993402448 0.0 0.38188919035549895 1.4077666486003281E-4 84-85 1.3536217775003153E-4 0.0010720684477802498 0.0 0.382143671249669 1.4077666486003281E-4 86-87 1.4077666486003281E-4 0.001093726396220255 0.0 0.38219240163365903 1.4077666486003281E-4 88-89 1.4077666486003281E-4 0.001137042293100265 0.0 0.3822303030434291 1.4077666486003281E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11895 0.0 48.5964 1 GTATCAA 21415 0.0 36.184612 1 ATCAACG 26260 0.0 28.853233 3 TCAACGC 26470 0.0 28.575617 4 CAACGCA 27030 0.0 27.999496 5 TATCAAC 27735 0.0 27.56729 2 AACGCAG 27765 0.0 27.320204 6 ACGCAGA 31875 0.0 23.810987 7 CGCAGAG 32140 0.0 23.547802 8 GCAGAGT 37590 0.0 19.973759 9 GTACATG 25490 0.0 19.55845 1 TACATGG 25045 0.0 19.408316 2 GTGGTAT 6035 0.0 19.227942 1 GAGTACT 23055 0.0 18.875248 12-13 AGAGTAC 34370 0.0 18.719965 10-11 ACATGGG 25540 0.0 18.661543 3 CAGAGTA 36775 0.0 17.939835 10-11 AGTACTT 25065 0.0 17.301596 12-13 TGGTATC 6170 0.0 17.135479 2 CATGGGG 18355 0.0 16.858347 4 >>END_MODULE