FastQCFastQC Report
Fri 27 May 2016
SRR2139031_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139031_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8818248
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA113850.049.678261
GTATCAA210200.036.6082841
TCAACGC259550.028.8729134
ATCAACG260000.028.8393843
CAACGCA266450.028.1412645
TATCAAC273700.027.6932242
AACGCAG274600.027.3527476
ACGCAGA314600.023.8342047
CGCAGAG320850.023.4097618
GCAGAGT370550.020.1202959
GTACATG246800.019.4524441
GAGTACT225150.019.34869612-13
AGAGTAC335400.019.17053410-11
TACATGG248850.018.9829732
CAGAGTA358000.018.13945410-11
ACATGGG254250.018.0917683
GTGGTAT58450.017.6267451
AGTACTT250500.017.28825612-13
GTACTTT254050.017.1060114-15
TGGTATC56900.016.6793962
Produced by FastQC (version 0.11.5)