##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139031_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8818248
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.667904554283346	34.0	31.0	34.0	31.0	34.0
2	32.77457302176124	34.0	31.0	34.0	31.0	34.0
3	32.84384029571407	34.0	31.0	34.0	31.0	34.0
4	36.303334857445606	37.0	37.0	37.0	35.0	37.0
5	36.24559810520185	37.0	37.0	37.0	35.0	37.0
6	36.282858567824356	37.0	37.0	37.0	35.0	37.0
7	36.23274691299224	37.0	37.0	37.0	35.0	37.0
8	36.21195287317843	37.0	37.0	37.0	35.0	37.0
9	37.945974415779645	39.0	38.0	39.0	35.0	39.0
10-11	37.902550483951	39.0	38.0	39.0	35.0	39.0
12-13	37.878179656548554	39.0	38.0	39.0	35.0	39.0
14-15	39.281468552483446	41.0	39.0	41.0	36.0	41.0
16-17	39.25454324940736	41.0	39.0	41.0	36.0	41.0
18-19	39.26792351496579	41.0	39.0	41.0	36.0	41.0
20-21	39.260769925524706	41.0	39.0	41.0	36.0	41.0
22-23	39.129935492864874	40.0	39.0	41.0	36.0	41.0
24-25	39.07928169502249	40.0	39.0	41.0	35.0	41.0
26-27	39.03152077419932	40.0	39.0	41.0	35.0	41.0
28-29	38.841347889523334	40.0	38.5	41.0	35.0	41.0
30-31	38.73320145784735	40.0	38.0	41.0	35.0	41.0
32-33	38.62029956231443	40.0	38.0	41.0	35.0	41.0
34-35	38.34355211532151	40.0	38.0	41.0	34.0	41.0
36-37	38.59131612852808	40.0	38.0	41.0	34.5	41.0
38-39	38.69526687040486	40.0	38.0	41.0	35.0	41.0
40-41	38.68442703773613	40.0	38.0	41.0	35.0	41.0
42-43	38.62650590894336	40.0	38.0	41.0	35.0	41.0
44-45	38.574899100080415	40.0	38.0	41.0	34.0	41.0
46-47	38.504531496671994	40.0	38.0	41.0	34.0	41.0
48-49	38.443158387344134	40.0	38.0	41.0	34.0	41.0
50-51	38.36934738697475	40.0	38.0	41.0	34.0	41.0
52-53	38.25761831209558	40.0	37.5	41.0	34.0	41.0
54-55	38.11497792252584	40.0	37.0	41.0	33.0	41.0
56-57	37.97264070506428	40.0	37.0	41.0	33.0	41.0
58-59	37.80701419909081	40.0	36.5	41.0	33.0	41.0
60-61	37.61084344885948	39.0	36.0	41.0	33.0	41.0
62-63	37.37597559834116	39.0	35.5	40.5	33.0	41.0
64-65	37.091231548654804	38.5	35.0	40.0	32.0	41.0
66-67	36.80586672982494	38.0	35.0	40.0	32.0	41.0
68-69	36.52244703658502	37.5	35.0	40.0	31.5	41.0
70-71	36.21475664786393	37.0	35.0	39.5	31.0	41.0
72-73	35.90885977278313	36.5	35.0	39.0	31.0	40.5
74-75	35.592082295116725	36.0	34.0	39.0	31.0	40.0
76-77	34.54054313382153	35.0	33.5	37.0	29.5	39.0
78-79	34.82126396817886	35.0	34.0	37.0	30.5	39.0
80-81	34.6201887415499	35.0	34.0	37.0	30.5	39.0
82-83	34.38264737741595	35.0	34.0	36.0	30.0	38.5
84-85	34.132119166790645	35.0	34.0	36.0	30.0	37.0
86-87	33.88897212854731	35.0	34.0	35.5	30.0	37.0
88-89	33.69986952607212	35.0	33.5	35.0	30.0	36.5
90-91	33.53682392687601	35.0	33.0	35.0	30.0	36.0
92-93	33.41408540758463	35.0	33.0	35.0	30.0	36.0
94-95	33.34010385366717	35.0	33.0	35.0	30.0	35.5
96-97	33.29328931165476	34.0	33.0	35.0	30.0	35.0
98-99	33.3070875362499	34.0	33.0	35.0	30.0	35.0
100-101	32.283877607527565	33.0	31.0	34.5	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	6.0
20	50.0
21	248.0
22	1189.0
23	3934.0
24	9595.0
25	19257.0
26	34400.0
27	53577.0
28	76458.0
29	102180.0
30	130880.0
31	164200.0
32	209035.0
33	272626.0
34	383367.0
35	554136.0
36	956315.0
37	1960035.0
38	2643018.0
39	1194241.0
40	49501.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.07037748776798	21.395365926511918	15.289401089513719	24.244855496206387
2	13.570189721442953	22.092345209606712	41.60211184150393	22.73535322744641
3	16.610754180678807	27.29506834976801	31.604508606871324	24.48966886268186
4	11.809685487305014	19.181571843377	39.63103769514228	29.3777049741757
5	12.683848919615079	38.26051121731011	36.07812710146666	12.977512761608148
6	28.77615344366669	39.04627972501561	19.262345368908964	12.915221462408738
7	25.370589570760444	33.33540479591973	24.102423543185346	17.191582090134478
8	23.9772562325362	36.966503519976776	21.654468465362704	17.401771782124325
9	25.35801896249686	16.85623947069758	21.686586723349127	36.09915484345643
10-11	23.503602983268333	27.128535055943086	29.65230735175513	19.71555460903345
12-13	24.546582268949567	24.88860599066844	29.583642918638713	20.981168821743275
14-15	21.707126903502644	26.152605448434425	27.322455092655414	24.817812555407524
16-17	21.146640831635196	29.261155674625122	27.90494126730083	21.68726222643885
18-19	21.165423439390523	28.43092200143441	29.734208465845047	20.669446093330016
20-21	22.035016588810695	27.549321886434008	29.607823413765853	20.80783811098944
22-23	21.827305812875853	27.51545400895215	29.354850947473693	21.302389230698303
24-25	21.515660084054538	27.669251165429436	29.451136138426005	21.363952612090017
26-27	21.475589668652486	27.79210379557382	29.400398843598698	21.331907692174997
28-29	21.414479625119288	27.879935620789357	29.38782777136834	21.31775698272301
30-31	21.65438794805958	27.726814579926877	29.30307728745133	21.315720184562213
32-33	21.514586607834026	27.85282956856699	29.239513236544102	21.393070587054886
34-35	21.631669064907104	27.9259730211827	29.1993645916942	21.242993322215998
36-37	21.58713889417546	27.893438118926777	29.143278971962538	21.376144014935228
38-39	21.486839164549124	27.93763136922603	29.192816476592697	21.38271298963215
40-41	21.663723349806855	27.84604113724981	29.025202012762076	21.465033500181267
42-43	21.612381878115674	27.968808334658764	28.839997692260077	21.578812094965485
44-45	21.697016091504658	28.01391051493121	28.63675232367543	21.6523210698887
46-47	21.762116399470518	28.10119430858662	28.339109461270773	21.79757983067209
48-49	21.76676985145333	28.200004374817016	28.290078732283796	21.743147041445862
50-51	21.79167992778518	28.274094412053515	28.102241223967507	21.8319844361938
52-53	21.88305777536567	28.19535569268711	28.038192912580236	21.883393619366988
54-55	21.870189247632467	28.120338406328216	28.05353376325483	21.95593858278449
56-57	21.857961578678644	28.19430205771599	28.042548719511455	21.905187644093903
58-59	21.840213076408503	28.24407850324315	28.063490757437513	21.852217662910835
60-61	21.93035768809388	28.18981420115552	28.034858865345814	21.844969245404783
62-63	21.89538264518924	28.264759327856538	28.00399757103168	21.835860455922543
64-65	21.871489951465055	28.249201023372727	27.961464859880998	21.917844165281224
66-67	21.926600791945063	28.24071378050352	27.819232246385095	22.013453181166323
68-69	21.861283400302653	28.238768150129935	27.899620222898363	22.000328226669044
70-71	21.862253885138607	28.055786883653855	27.95143967612171	22.130519555085826
72-73	21.91398813769506	28.065688809244072	27.898222310558157	22.12210074250271
74-75	21.840960865513516	28.160607690186705	27.83880752526235	22.15962391903743
76-77	21.984923227056353	28.071659156095862	27.8254291503957	22.11798846645209
78-79	21.935231929856748	28.037065108472998	27.780367308396237	22.24733565327402
80-81	21.971038572870647	28.07681262694407	27.758973572765722	22.193175227419566
82-83	21.971941360342157	28.05923921649675	27.857314915361712	22.111504507799378
84-85	22.08455940174807	28.029344007133627	27.82215997170754	22.06393661941076
86-87	21.96474225127321	27.99863264347746	27.894686969103894	22.141938136145434
88-89	21.976510977222627	27.961282846817998	27.876041546482483	22.186164629476895
90-91	22.071151024829224	28.019630427162102	27.843624284307346	22.06559426370133
92-93	22.087758090207416	27.891383928914433	27.923277280679727	22.097580700198428
94-95	21.96763024437553	27.91767781002922	27.97139109287391	22.14330085272133
96-97	21.97826395340363	27.958805726885377	27.977454637500294	22.085475682210703
98-99	22.161737235354742	28.505904338909517	28.08538820858944	21.246970217146306
100-101	22.696651157259282	31.216878395808294	25.267477723752158	20.818992723180262
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2184.0
1	1899.5
2	2499.0
3	3576.5
4	4871.0
5	7523.5
6	9706.5
7	11620.5
8	13158.0
9	13306.0
10	12246.0
11	11170.0
12	12197.5
13	16385.5
14	26403.5
15	39879.0
16	50706.0
17	56642.5
18	57329.5
19	54279.5
20	49943.5
21	45866.5
22	44209.5
23	47786.0
24	56058.5
25	68767.5
26	85132.0
27	103638.0
28	125382.0
29	148940.5
30	171324.0
31	193442.0
32	215875.5
33	237097.0
34	255642.0
35	270993.5
36	287416.5
37	304236.5
38	317641.0
39	327508.0
40	336183.5
41	344307.0
42	349177.0
43	354225.0
44	358341.5
45	359545.5
46	358555.5
47	354386.0
48	350064.5
49	342997.5
50	333458.0
51	322575.0
52	306172.0
53	286993.5
54	267730.0
55	246272.0
56	224086.5
57	201182.0
58	176773.0
59	151753.0
60	126508.0
61	103003.0
62	81826.5
63	62915.0
64	47079.0
65	35444.0
66	27043.0
67	20639.5
68	15766.0
69	11409.0
70	7948.5
71	5794.0
72	4105.0
73	2826.5
74	1924.0
75	1274.0
76	829.5
77	501.5
78	324.0
79	218.5
80	149.0
81	103.0
82	74.0
83	51.5
84	40.5
85	30.0
86	23.0
87	18.0
88	12.0
89	7.0
90	3.0
91	2.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.2680242152409415E-5
2	0.0018370996143451622
3	3.402036322861412E-5
4	3.402036322861412E-5
5	2.2680242152409415E-5
6	2.2680242152409415E-5
7	2.2680242152409415E-5
8	9.072096860963766E-5
9	0.0
10-11	0.0
12-13	0.0
14-15	7.200976883389989E-4
16-17	0.0028010099058225625
18-19	2.835030269051177E-5
20-21	5.670224827249513E-6
22-23	0.0010093793456828815
24-25	0.00566616230826861
26-27	0.009968961182612703
28-29	0.36439817252562434
30-31	0.33394543688310774
32-33	0.33697083864589816
34-35	0.1903726810947757
36-37	0.048017942297263694
38-39	0.037519570037030534
40-41	0.023669784513276056
42-43	0.0310408036389265
44-45	0.019956098939816036
46-47	0.015333186390297094
48-49	0.009151423857599759
50-51	0.0049628257687607995
52-53	0.003785734509736085
54-55	5.78178817914637E-4
56-57	0.0035773224293404425
58-59	0.0062832559294554785
60-61	0.004899458631890809
62-63	0.003955693686852788
64-65	4.362730502139374E-4
66-67	8.420943534334778E-5
68-69	7.852250998250125E-4
70-71	0.0011040161728453008
72-73	0.0018900148910466522
74-75	0.0021183486298568285
76-77	9.680848996822024E-4
78-79	9.648988410393256E-5
80-81	0.0032278561053817635
82-83	0.0015376679628925454
84-85	7.135905751240328E-5
86-87	2.1667381569884133E-5
88-89	0.0015144349796729173
90-91	0.0038280093216478154
92-93	0.015695633646437626
94-95	0.0018067425488329592
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	141.0
20-21	565.0
22-23	1162.0
24-25	3167.0
26-27	3921.0
28-29	6526.0
30-31	17332.0
32-33	23196.0
34-35	32915.0
36-37	45690.0
38-39	53068.0
40-41	57931.0
42-43	69009.0
44-45	82638.0
46-47	81265.0
48-49	71950.0
50-51	64613.0
52-53	58683.0
54-55	63089.0
56-57	67104.0
58-59	68112.0
60-61	67643.0
62-63	67356.0
64-65	72920.0
66-67	77636.0
68-69	77930.0
70-71	77287.0
72-73	74960.0
74-75	77145.0
76-77	78387.0
78-79	82048.0
80-81	83627.0
82-83	82008.0
84-85	83094.0
86-87	87231.0
88-89	92715.0
90-91	100106.0
92-93	104408.0
94-95	117018.0
96-97	172957.0
98-99	741999.0
100-101	5527696.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.87243358546709
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.27672463235392	75.77132882087493
2	7.451607910976257	12.648721950585474
3	1.8476654051056203	4.704475781489757
4	0.6914188893007117	2.3472961504764833
5	0.3005234343705828	1.2753077612246884
6	0.15878508742577122	0.8085886072143811
7	0.08436321223845748	0.5012077789435604
8	0.05032292732589599	0.34168234458347146
9	0.036045320816140355	0.2753328687331254
>10	0.10139849813558521	1.2477732253519211
>50	9.63472851839086E-4	0.048565821558327976
>100	1.81209099320219E-4	0.029718888963926576
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	1.1340121076204706E-5	2.268024215240941E-5	0.0	0.0	0.0
3	1.1340121076204706E-5	2.268024215240941E-5	0.0	0.0	0.0
4	1.1340121076204706E-5	2.268024215240941E-5	0.0	1.1340121076204706E-5	1.1340121076204706E-5
5	1.1340121076204706E-5	2.268024215240941E-5	0.0	1.1340121076204706E-5	1.1340121076204706E-5
6	6.804072645722824E-5	2.268024215240941E-5	0.0	1.1340121076204706E-5	2.268024215240941E-5
7	6.804072645722824E-5	2.268024215240941E-5	0.0	1.1340121076204706E-5	2.268024215240941E-5
8	6.804072645722824E-5	2.268024215240941E-5	0.0	1.1340121076204706E-5	3.402036322861412E-5
9	7.938084753343295E-5	7.938084753343295E-5	0.0	4.536048430481882E-5	3.402036322861412E-5
10-11	9.072096860963765E-5	9.072096860963765E-5	0.0	6.237066591912589E-5	3.402036322861412E-5
12-13	1.0206108968584235E-4	9.072096860963765E-5	0.0	6.804072645722824E-5	3.402036322861412E-5
14-15	1.1340121076204706E-4	1.3608145291445648E-4	0.0	1.0773115022394471E-4	3.969042376671647E-5
16-17	1.6443175560496825E-4	1.3608145291445648E-4	0.0	1.2474133183825177E-4	6.237066591912589E-5
18-19	1.701018161430706E-4	1.474215739906612E-4	0.0	1.3608145291445648E-4	9.072096860963765E-5
20-21	1.701018161430706E-4	1.587616950668659E-4	0.0	1.8711199775737765E-4	9.072096860963765E-5
22-23	1.701018161430706E-4	1.9278205829548E-4	0.0	3.0618326905752704E-4	1.0773115022394471E-4
24-25	1.701018161430706E-4	2.6082278475270825E-4	0.0	0.0010659713811632424	1.4175151345255884E-4
26-27	1.701018161430706E-4	2.835030269051177E-4	0.0	0.0035154375336234594	1.587616950668659E-4
28-29	1.701018161430706E-4	2.835030269051177E-4	0.0	0.01775862960533657	1.587616950668659E-4
30-31	1.7577187668117294E-4	3.628838744385506E-4	0.0	0.024834865156888306	1.6443175560496825E-4
32-33	1.814419372192753E-4	3.628838744385506E-4	0.0	0.0281915409954449	1.701018161430706E-4
34-35	1.814419372192753E-4	4.365946614338812E-4	0.0	0.032755939728617296	1.9845211883358235E-4
36-37	1.814419372192753E-4	5.103054484292118E-4	0.0	0.039310529710663615	2.1546230044788942E-4
38-39	1.814419372192753E-4	5.216455695054165E-4	0.0	0.05192074434740325	2.1546230044788942E-4
40-41	1.9278205829548E-4	5.670060538102353E-4	0.0	0.07987414280024785	2.2113236098599177E-4
42-43	1.9278205829548E-4	5.896862959626448E-4	0.0	0.09176425974864849	2.3247248206219648E-4
44-45	1.9845211883358235E-4	6.804072645722823E-4	0.0	0.10851928863874094	2.3814254260029884E-4
46-47	2.2680242152409413E-4	7.597881121057153E-4	0.0	0.1278938854974367	2.551527242146059E-4
48-49	2.3814254260029884E-4	7.767982937200224E-4	0.0	0.14548808334716828	2.948431479813224E-4
50-51	2.6082278475270825E-4	8.788593834058647E-4	0.0	0.16186321818120786	2.948431479813224E-4
52-53	2.6082278475270825E-4	9.185498071725812E-4	0.0	0.18585891437845703	3.175233901337318E-4
54-55	2.6082278475270825E-4	9.242198677106836E-4	0.0	0.23916882355769536	3.628838744385506E-4
56-57	2.7216290582891296E-4	9.412300493249906E-4	0.0	0.2922235800127191	3.969042376671647E-4
58-59	2.835030269051177E-4	9.695803520155024E-4	0.0	0.33036607725253364	4.082443587433694E-4
60-61	3.0051320851942474E-4	9.865905336298095E-4	0.0	0.3559493904004514	4.082443587433694E-4
62-63	3.402036322861412E-4	0.0010319510179346283	0.0	0.3728461708039964	4.082443587433694E-4
64-65	3.402036322861412E-4	0.0011113318654680613	0.0	0.38565483755956964	4.139144192814718E-4
66-67	3.458736928242435E-4	0.0011340121076204707	0.0	0.3934681809810747	4.252545403576765E-4
68-69	3.515437533623459E-4	0.0011510222892347779	0.0	0.40134956512903697	4.3092460089577884E-4
70-71	3.515437533623459E-4	0.0011737025313871873	0.0	0.40772838323440214	4.3092460089577884E-4
72-73	3.7989405605285765E-4	0.0011850426524633918	0.0	0.4122814418464983	5.216455695054165E-4
74-75	3.8556411659096E-4	0.0012304031367682106	0.0	0.4160463620437983	5.216455695054165E-4
76-77	3.8556411659096E-4	0.001281433681611132	0.0	0.41931231691374526	5.216455695054165E-4
78-79	3.9123417712906236E-4	0.0013041139237635413	0.0	0.4222267280303298	5.216455695054165E-4
80-81	3.969042376671647E-4	0.0013041139237635413	0.0	0.4233664101984884	5.499958721959283E-4
82-83	3.969042376671647E-4	0.0013041139237635413	0.0	0.4238256851020747	5.896862959626448E-4
84-85	4.0257429820526706E-4	0.0013211241053778483	0.0	0.4241261983105941	5.896862959626448E-4
86-87	4.139144192814718E-4	0.0013381342869921555	0.0	0.42422825940028	5.896862959626448E-4
88-89	4.1958447981957413E-4	0.001383494771296974	0.0	0.4242679498240467	6.123665381150542E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11385	0.0	49.67826	1
GTATCAA	21020	0.0	36.608284	1
TCAACGC	25955	0.0	28.872913	4
ATCAACG	26000	0.0	28.839384	3
CAACGCA	26645	0.0	28.141264	5
TATCAAC	27370	0.0	27.693224	2
AACGCAG	27460	0.0	27.352747	6
ACGCAGA	31460	0.0	23.834204	7
CGCAGAG	32085	0.0	23.409761	8
GCAGAGT	37055	0.0	20.120295	9
GTACATG	24680	0.0	19.452444	1
GAGTACT	22515	0.0	19.348696	12-13
AGAGTAC	33540	0.0	19.170534	10-11
TACATGG	24885	0.0	18.982973	2
CAGAGTA	35800	0.0	18.139454	10-11
ACATGGG	25425	0.0	18.091768	3
GTGGTAT	5845	0.0	17.626745	1
AGTACTT	25050	0.0	17.288256	12-13
GTACTTT	25405	0.0	17.10601	14-15
TGGTATC	5690	0.0	16.679396	2
>>END_MODULE