FastQCFastQC Report
Fri 27 May 2016
SRR2139030_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139030_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8288760
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA119200.051.5567971
GTATCAA222000.037.7666321
ATCAACG274050.030.0186963
TCAACGC276250.029.795044
CAACGCA280000.029.4111985
TATCAAC285100.029.064562
AACGCAG286750.028.7633956
ACGCAGA332100.024.8227867
CGCAGAG336200.024.520078
GCAGAGT385250.021.2992679
GTACATG270800.020.6824631
TACATGG274450.020.2368222
ACATGGG270300.020.0434343
GAGTACT226800.019.7612512-13
AGAGTAC354650.018.98609210-11
CAGAGTA379300.018.76214610-11
CATGGGG187300.018.3823284
GTACTTT254450.017.6311914-15
AGTACTT249300.017.28629112-13
GTGGTAT57150.016.6811811
Produced by FastQC (version 0.11.5)