##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139030_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8288760
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.67437867666575	34.0	31.0	34.0	31.0	34.0
2	32.77645063917884	34.0	31.0	34.0	31.0	34.0
3	32.84426946853329	34.0	31.0	34.0	31.0	34.0
4	36.303882848580486	37.0	37.0	37.0	35.0	37.0
5	36.248271635323015	37.0	37.0	37.0	35.0	37.0
6	36.286678345132444	37.0	37.0	37.0	35.0	37.0
7	36.23504432508602	37.0	37.0	37.0	35.0	37.0
8	36.215703917111846	37.0	37.0	37.0	35.0	37.0
9	37.947334462573416	39.0	38.0	39.0	35.0	39.0
10-11	37.904616372050825	39.0	38.0	39.0	35.0	39.0
12-13	37.88052627896091	39.0	38.0	39.0	35.0	39.0
14-15	39.276533522505176	41.0	39.0	41.0	36.0	41.0
16-17	39.251920552652024	41.0	39.0	41.0	36.0	41.0
18-19	39.27101635226499	41.0	39.0	41.0	36.0	41.0
20-21	39.26498300867847	41.0	39.0	41.0	36.0	41.0
22-23	39.132728597616165	40.0	39.0	41.0	36.0	41.0
24-25	39.08204736671048	40.0	39.0	41.0	35.0	41.0
26-27	39.03146846997714	40.0	39.0	41.0	35.0	41.0
28-29	38.84147034261364	40.0	38.5	41.0	35.0	41.0
30-31	38.73033980671868	40.0	38.0	41.0	35.0	41.0
32-33	38.613901670029605	40.0	38.0	41.0	35.0	41.0
34-35	38.33538990183935	40.0	38.0	41.0	34.0	41.0
36-37	38.58650468596224	40.0	38.0	41.0	34.5	41.0
38-39	38.690704382629804	40.0	38.0	41.0	35.0	41.0
40-41	38.681933229704654	40.0	38.0	41.0	35.0	41.0
42-43	38.62699999644513	40.0	38.0	41.0	35.0	41.0
44-45	38.57981661657676	40.0	38.0	41.0	34.0	41.0
46-47	38.51122339096713	40.0	38.0	41.0	34.0	41.0
48-49	38.45300107452637	40.0	38.0	41.0	34.0	41.0
50-51	38.38158663063799	40.0	38.0	41.0	34.0	41.0
52-53	38.27411180179017	40.0	38.0	41.0	34.0	41.0
54-55	38.137674354920456	40.0	37.0	41.0	33.5	41.0
56-57	37.99749030083157	40.0	37.0	41.0	33.0	41.0
58-59	37.83372611119994	40.0	36.5	41.0	33.0	41.0
60-61	37.640607636333925	39.0	36.0	41.0	33.0	41.0
62-63	37.40678236599584	39.0	36.0	40.5	33.0	41.0
64-65	37.12522423266164	39.0	35.0	40.0	32.0	41.0
66-67	36.84350141316243	38.0	35.0	40.0	32.0	41.0
68-69	36.558618861942264	37.5	35.0	40.0	32.0	41.0
70-71	36.25160671740892	37.0	35.0	39.5	31.0	41.0
72-73	35.94807068654242	36.5	35.0	39.0	31.0	40.5
74-75	35.6289559826584	36.0	34.0	39.0	31.0	40.0
76-77	34.57718509028995	35.0	33.5	37.0	29.5	39.0
78-79	34.853116366714175	35.0	34.0	37.0	30.5	39.0
80-81	34.65117253548597	35.0	34.0	37.0	31.0	39.0
82-83	34.41290648141833	35.0	34.0	36.5	30.0	38.5
84-85	34.16015598884833	35.0	34.0	36.0	30.0	37.0
86-87	33.91684759800577	35.0	34.0	35.5	30.0	37.0
88-89	33.72360683518351	35.0	33.5	35.0	30.0	36.5
90-91	33.558135113001995	35.0	33.0	35.0	30.0	36.0
92-93	33.43353255257691	35.0	33.0	35.0	30.0	36.0
94-95	33.35746762697555	35.0	33.0	35.0	30.0	36.0
96-97	33.308836724266015	34.0	33.0	35.0	30.0	35.0
98-99	33.320494857868574	34.0	33.0	35.0	30.0	35.0
100-101	32.299283862040475	33.0	31.0	34.5	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.0
17	0.0
18	3.0
19	11.0
20	30.0
21	229.0
22	1034.0
23	3372.0
24	8528.0
25	17332.0
26	30834.0
27	48321.0
28	69692.0
29	94273.0
30	121824.0
31	154048.0
32	194587.0
33	255128.0
34	360578.0
35	522656.0
36	893245.0
37	1832128.0
38	2484992.0
39	1147140.0
40	48773.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.09031968360036	21.458908560245092	15.312885235640852	24.13788652051369
2	13.60145153444342	22.211401480200294	41.526043938861626	22.66110304649466
3	16.593376644199694	27.505721906017534	31.63274502250578	24.268156427276992
4	11.774260993022114	19.27627757982559	39.69066294371243	29.258798483439858
5	12.568662277267595	38.42727704198868	36.04133454010842	12.962726140635302
6	28.512727721089213	39.18334930275441	19.337336184745652	12.966586791410728
7	25.357861170773578	33.398244538175135	24.10413911177777	17.139755179273518
8	24.003037850678417	36.8381499996079	21.727678040912053	17.431134108801626
9	25.544556725010736	16.752421351323964	21.75271090006225	35.95031102360305
10-11	23.491428361505722	27.225358631771137	29.612151251342254	19.671061755380887
12-13	24.567860572630888	25.030414682051354	29.56532098890546	20.8364037564123
14-15	21.75385241259729	26.24803618226352	27.432564230201546	24.565547174937645
16-17	21.012640619227707	29.308339560823782	28.052753218367865	21.626266601580642
18-19	21.04408321877049	28.454538709371914	29.9825667425325	20.518811329325093
20-21	21.96377246018309	27.535461478498945	29.868899092851652	20.631866968466312
22-23	21.774561805550988	27.501250191472803	29.610168911742484	21.114019091233722
24-25	21.430479224558603	27.706470250865607	29.708177560167385	21.154872964408405
26-27	21.35502705768932	27.81850553122476	29.61970720571831	21.206760205367612
28-29	21.26624890029312	27.91289727498182	29.666985056836825	21.15386876788823
30-31	21.54894145099497	27.740665685656907	29.584445139002806	21.125947724345316
32-33	21.351530215587708	27.890655506826718	29.523776863032815	21.234037414552766
34-35	21.504908197690124	27.927683832995825	29.46231624252053	21.10509172679352
36-37	21.50121911188806	27.86789926486866	29.42220050840859	21.20868111483469
38-39	21.386136643386685	28.027813863430325	29.383322627928766	21.202726865254228
40-41	21.532991125200176	27.93463588071195	29.263827978773087	21.26854501531479
42-43	21.45550892239906	28.079792721776354	29.017587014797495	21.447111341027085
44-45	21.582936973992734	28.159727543999562	28.819939470148164	21.437396011859537
46-47	21.612189658189266	28.136933199433923	28.530511568012002	21.720365574364813
48-49	21.629011216156687	28.371835925587067	28.35975132025757	21.63940153799867
50-51	21.644994763596486	28.36390848214488	28.261032286989362	21.730064467269273
52-53	21.71115912757116	28.34923783388111	28.11722499671206	21.822378041835673
54-55	21.76421598961237	28.288559381977347	28.144712048509803	21.80251257990048
56-57	21.786596014208296	28.292431791272172	28.09434506253262	21.82662713198691
58-59	21.691567765932344	28.435934316962875	28.134603457953876	21.737894459150905
60-61	21.86343666766698	28.36232931405485	28.0840181060869	21.69021591219127
62-63	21.788010916063612	28.447048002698722	28.009506792584236	21.755434288653426
64-65	21.778304818063248	28.374957287891363	28.006228308191066	21.840509585854324
66-67	21.865281702698073	28.404074408394152	27.850021751362842	21.880622137544933
68-69	21.73114407197033	28.359437181282175	28.01618116783461	21.89323757891288
70-71	21.72683195094964	28.23299204691577	28.027226866774168	22.01294913536043
72-73	21.797704513061138	28.205742604751038	27.96766596333054	22.02888691885728
74-75	21.742814050080636	28.312797500453602	27.896380062083526	22.048008387382232
76-77	21.876167545124968	28.217484702393687	27.890321601664407	22.016026150816938
78-79	21.84437731759205	28.136676700198542	27.874058173187276	22.144887809022133
80-81	21.828607090729566	28.202937777177468	27.894530663923874	22.073924468169093
82-83	21.87401151083212	28.185537329553895	27.954922357051164	21.985528802562822
84-85	21.994235128380776	28.14655308174943	27.88901675702713	21.970195032842664
86-87	21.816601858676552	28.179755092450804	27.960047043826847	22.0435960050458
88-89	21.917749304067954	28.062456780664156	27.932599261472706	22.087194653795184
90-91	21.93589682746365	28.100693562364583	27.964780702926312	21.998628907245454
92-93	22.036997705504604	28.050242693555827	27.972844177954865	21.93991542298471
94-95	21.8871992776422	28.06305051909998	28.02132852639766	22.02842167686016
96-97	21.818537552631838	28.097090354688458	28.101136051929963	21.98323604074974
98-99	22.073435961091953	28.653615868305643	28.129050848859823	21.14389732174258
100-101	22.62543391858586	31.29315780678972	25.389283747237947	20.69212452738648
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2396.0
1	2103.5
2	2896.0
3	4163.5
4	5524.5
5	8338.5
6	10574.0
7	12709.0
8	14429.0
9	14409.5
10	13034.5
11	11668.5
12	12919.5
13	17578.5
14	28430.5
15	43380.0
16	55077.0
17	60744.5
18	60793.5
19	56519.5
20	50641.0
21	45270.5
22	42528.5
23	45224.5
24	52957.0
25	65189.0
26	81132.5
27	98779.0
28	117587.0
29	138284.0
30	158826.0
31	180250.5
32	203678.0
33	227697.0
34	248280.0
35	264492.0
36	283173.0
37	300273.5
38	309981.5
39	315882.5
40	321267.5
41	325859.0
42	328576.0
43	332616.0
44	336860.0
45	337981.5
46	336294.0
47	332870.5
48	329844.5
49	323131.0
50	310736.5
51	296303.0
52	279719.0
53	262610.5
54	246366.0
55	226712.0
56	206403.0
57	185623.5
58	162899.5
59	139882.5
60	115928.0
61	93379.5
62	73188.0
63	55533.5
64	41519.5
65	31310.0
66	23766.5
67	18159.0
68	13953.0
69	10246.0
70	7331.5
71	5274.0
72	3630.5
73	2542.0
74	1749.5
75	1183.5
76	777.0
77	505.0
78	343.0
79	215.5
80	137.0
81	90.0
82	59.5
83	43.0
84	28.5
85	17.0
86	13.5
87	12.5
88	8.5
89	6.5
90	5.0
91	3.0
92	2.5
93	1.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.4129061524281074E-5
2	0.0018338086758453618
3	1.2064530762140537E-5
4	2.4129061524281074E-5
5	2.4129061524281074E-5
6	2.4129061524281074E-5
7	1.2064530762140537E-5
8	6.032265381070269E-5
9	0.0
10-11	6.0322653810702685E-6
12-13	0.0
14-15	6.997427842041511E-4
16-17	0.00291961644443801
18-19	6.032265381070269E-5
20-21	0.0
22-23	0.0010316348722027612
24-25	0.005491185320022057
26-27	0.01002069756973334
28-29	0.36044013812191233
30-31	0.3299379342279134
32-33	0.3331960697153734
34-35	0.18793763519528847
36-37	0.04569618652931847
38-39	0.0360129595075564
40-41	0.02208536398045881
42-43	0.02965061597426468
44-45	0.01813982220208642
46-47	0.013842516880850869
48-49	0.00790603117235148
50-51	0.004053240675992487
52-53	0.0026563905904510134
54-55	3.617215948581341E-4
56-57	0.002552855209412702
58-59	0.004842463428030693
60-61	0.0032323058919335543
62-63	0.002866116417700318
64-65	2.1982862435231275E-4
66-67	1.1794983482513279E-4
68-69	8.903460964563104E-4
70-71	0.0012044507715109416
72-73	0.0020259908133128932
74-75	0.0021920034412283725
76-77	9.506638375885901E-4
78-79	1.1831981135089279E-4
80-81	0.0031502718587337283
82-83	0.0014766773578107372
84-85	3.065360302066735E-5
86-87	1.5518842629546836E-5
88-89	0.0013995104701073605
90-91	0.003477461622103448
92-93	0.015274861659643686
94-95	0.0016714866688234058
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	154.0
20-21	567.0
22-23	1119.0
24-25	3168.0
26-27	4027.0
28-29	6653.0
30-31	17336.0
32-33	22945.0
34-35	31373.0
36-37	44926.0
38-39	53258.0
40-41	58545.0
42-43	69703.0
44-45	83017.0
46-47	82122.0
48-49	71480.0
50-51	63345.0
52-53	57439.0
54-55	60956.0
56-57	64622.0
58-59	66047.0
60-61	65664.0
62-63	64613.0
64-65	70635.0
66-67	74461.0
68-69	74812.0
70-71	73479.0
72-73	72562.0
74-75	74021.0
76-77	74741.0
78-79	79036.0
80-81	79720.0
82-83	78146.0
84-85	79984.0
86-87	82978.0
88-89	88902.0
90-91	95027.0
92-93	97865.0
94-95	109719.0
96-97	160270.0
98-99	689244.0
100-101	5140079.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.41902425451376
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.47425053765649	75.53328926292863
2	7.202157178072854	12.159981630011055
3	1.8493536744907195	4.683618981060186
4	0.6869738303653925	2.3197464179132914
5	0.29470679178951054	1.2439429902024313
6	0.16305363920389776	0.8258897473644347
7	0.09554137113429025	0.5645856528966604
8	0.06080998392653643	0.41068156064086964
9	0.039178912995484164	0.2976701045788136
>10	0.1304527058413287	1.6851259511180217
>50	0.0028659830092417483	0.15104257175796384
>100	6.075734092739896E-4	0.09901377034116868
>500	4.781810502416388E-5	0.025411359186503425
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.4129061524281074E-5	0.0
5	0.0	0.0	0.0	2.4129061524281074E-5	0.0
6	0.0	0.0	0.0	2.4129061524281074E-5	1.2064530762140537E-5
7	0.0	0.0	0.0	2.4129061524281074E-5	1.2064530762140537E-5
8	1.2064530762140537E-5	0.0	0.0	3.619359228642161E-5	1.2064530762140537E-5
9	1.2064530762140537E-5	2.4129061524281074E-5	0.0	4.825812304856215E-5	1.2064530762140537E-5
10-11	4.825812304856215E-5	3.0161326905351345E-5	0.0	9.65162460971243E-5	1.2064530762140537E-5
12-13	6.032265381070268E-5	3.619359228642161E-5	0.0	9.65162460971243E-5	1.2064530762140537E-5
14-15	6.032265381070268E-5	4.825812304856215E-5	0.0	1.0254851147819456E-4	1.8096796143210806E-5
16-17	6.635491919177296E-5	4.825812304856215E-5	0.0	1.3270983838354592E-4	2.4129061524281074E-5
18-19	7.238718457284323E-5	5.4290388429632416E-5	0.0	1.749356960510378E-4	2.4129061524281074E-5
20-21	7.238718457284323E-5	6.032265381070268E-5	0.0	2.5335514600495127E-4	3.0161326905351345E-5
22-23	9.65162460971243E-5	7.238718457284323E-5	0.0	5.790974765827458E-4	3.619359228642161E-5
24-25	1.0858077685926483E-4	8.445171533498376E-5	0.0	0.0015985503259836212	4.825812304856215E-5
26-27	1.2064530762140537E-4	9.65162460971243E-5	0.0	0.004862005897142637	5.4290388429632416E-5
28-29	1.2064530762140537E-4	1.0254851147819456E-4	0.0	0.019580733426954093	6.032265381070268E-5
30-31	1.5683889990782699E-4	1.146130422403351E-4	0.0	0.02719345233786477	6.635491919177296E-5
32-33	1.6287116528889724E-4	1.2064530762140537E-4	0.0	0.030794714770363725	7.238718457284323E-5
34-35	1.870002268131783E-4	1.3270983838354592E-4	0.0	0.03545765590993104	7.238718457284323E-5
36-37	2.0509702295638913E-4	1.4477436914568645E-4	0.0	0.04203282517529763	7.238718457284323E-5
38-39	2.0509702295638913E-4	1.4477436914568645E-4	0.0	0.05482726004854767	7.238718457284323E-5
40-41	2.1716155371852966E-4	1.6890343066996752E-4	0.0	0.08375800481616068	7.238718457284323E-5
42-43	2.1716155371852966E-4	1.870002268131783E-4	0.0	0.0959492131513037	7.238718457284323E-5
44-45	2.2319381909959994E-4	2.4129061524281073E-4	0.0	0.11314720175273503	7.238718457284323E-5
46-47	2.292260844806702E-4	2.7748420752923234E-4	0.0	0.13330703265627186	7.238718457284323E-5
48-49	2.292260844806702E-4	2.7748420752923234E-4	0.0	0.1505472471153707	8.445171533498376E-5
50-51	2.292260844806702E-4	2.9558100367244316E-4	0.0	0.16731694487474605	8.445171533498376E-5
52-53	2.292260844806702E-4	3.016132690535134E-4	0.0	0.1930143953981054	1.146130422403351E-4
54-55	2.292260844806702E-4	3.0764553443458366E-4	0.0	0.24793213942736914	1.3270983838354592E-4
56-57	2.292260844806702E-4	3.1367779981565397E-4	0.0	0.30337469054478594	1.3270983838354592E-4
58-59	2.292260844806702E-4	3.1367779981565397E-4	0.0	0.343983901090151	1.3270983838354592E-4
60-61	2.4129061524281073E-4	3.1367779981565397E-4	0.0	0.37001915847485023	1.3270983838354592E-4
62-63	2.4129061524281073E-4	3.1367779981565397E-4	0.0	0.3868491788880363	1.3270983838354592E-4
64-65	2.4129061524281073E-4	3.498713921020756E-4	0.0	0.3994867748613785	1.3270983838354592E-4
66-67	2.4129061524281073E-4	3.498713921020756E-4	0.0	0.40759413953353696	1.3270983838354592E-4
68-69	2.4129061524281073E-4	3.498713921020756E-4	0.0	0.41526718109825833	1.3270983838354592E-4
70-71	2.4129061524281073E-4	3.498713921020756E-4	0.0	0.42233699612487274	1.3270983838354592E-4
72-73	2.4129061524281073E-4	3.5590365748314586E-4	0.0	0.42716884069510996	1.6890343066996752E-4
74-75	2.4129061524281073E-4	3.740004536263567E-4	0.0	0.4312768134196189	1.6890343066996752E-4
76-77	2.5335514600495127E-4	3.8003271900742693E-4	0.0	0.43438343009087005	1.6890343066996752E-4
78-79	2.6541967676709183E-4	3.860649843884972E-4	0.0	0.4371341431046381	1.6890343066996752E-4
80-81	2.6541967676709183E-4	3.860649843884972E-4	0.0	0.43838885430390073	1.8096796143210806E-4
82-83	2.6541967676709183E-4	3.9812951515063775E-4	0.0	0.43887143553438634	1.930324921942486E-4
84-85	2.6541967676709183E-4	3.9812951515063775E-4	0.0	0.43920924239572623	1.930324921942486E-4
86-87	2.6541967676709183E-4	4.1019404591277826E-4	0.0	0.43928766184568013	1.930324921942486E-4
88-89	2.714519421481621E-4	4.584521689613404E-4	0.0	0.43929369411106123	1.930324921942486E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11920	0.0	51.556797	1
GTATCAA	22200	0.0	37.766632	1
ATCAACG	27405	0.0	30.018696	3
TCAACGC	27625	0.0	29.79504	4
CAACGCA	28000	0.0	29.411198	5
TATCAAC	28510	0.0	29.06456	2
AACGCAG	28675	0.0	28.763395	6
ACGCAGA	33210	0.0	24.822786	7
CGCAGAG	33620	0.0	24.52007	8
GCAGAGT	38525	0.0	21.299267	9
GTACATG	27080	0.0	20.682463	1
TACATGG	27445	0.0	20.236822	2
ACATGGG	27030	0.0	20.043434	3
GAGTACT	22680	0.0	19.76125	12-13
AGAGTAC	35465	0.0	18.986092	10-11
CAGAGTA	37930	0.0	18.762146	10-11
CATGGGG	18730	0.0	18.382328	4
GTACTTT	25445	0.0	17.63119	14-15
AGTACTT	24930	0.0	17.286291	12-13
GTGGTAT	5715	0.0	16.681181	1
>>END_MODULE