FastQCFastQC Report
Fri 27 May 2016
SRR2139029_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139029_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9863993
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA131300.050.4513781
GTATCAA242750.037.0670741
ATCAACG299450.029.3614083
TCAACGC300950.029.2150634
CAACGCA305400.028.817435
TATCAAC315500.028.207552
AACGCAG314500.028.0380976
ACGCAGA357100.024.6693157
CGCAGAG363900.024.2083348
GCAGAGT421100.020.6962539
GTACATG286600.019.7344931
TACATGG283750.019.419292
GAGTACT257400.019.38647512-13
AGAGTAC384500.019.33062210-11
ACATGGG281250.018.9670893
CAGAGTA409900.018.58753210-11
AGTACTT284200.017.52817712-13
CATGGGG207650.017.4360714
GTGGTAT68200.017.2773271
GTACTTT290600.017.17949114-15
Produced by FastQC (version 0.11.5)