##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139029_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9863993
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.6522868578678	34.0	31.0	34.0	31.0	34.0
2	32.76088162268566	34.0	31.0	34.0	31.0	34.0
3	32.828733353724	34.0	31.0	34.0	31.0	34.0
4	36.294863550693925	37.0	37.0	37.0	35.0	37.0
5	36.23546113627615	37.0	37.0	37.0	35.0	37.0
6	36.27253800768107	37.0	37.0	37.0	35.0	37.0
7	36.22118821454963	37.0	37.0	37.0	35.0	37.0
8	36.19827660056125	37.0	37.0	37.0	35.0	37.0
9	37.926710004761766	39.0	38.0	39.0	35.0	39.0
10-11	37.880977764278626	39.0	38.0	39.0	35.0	39.0
12-13	37.85818587868017	39.0	38.0	39.0	35.0	39.0
14-15	39.25584132105528	41.0	39.0	41.0	36.0	41.0
16-17	39.2291641934458	41.0	39.0	41.0	36.0	41.0
18-19	39.24023425401863	41.0	39.0	41.0	36.0	41.0
20-21	39.232584825703185	40.5	39.0	41.0	36.0	41.0
22-23	39.1036098757545	40.0	39.0	41.0	35.5	41.0
24-25	39.05469799697773	40.0	39.0	41.0	35.0	41.0
26-27	39.00658738522241	40.0	38.5	41.0	35.0	41.0
28-29	38.819425292104256	40.0	38.5	41.0	35.0	41.0
30-31	38.70959919708645	40.0	38.0	41.0	35.0	41.0
32-33	38.59715756653074	40.0	38.0	41.0	35.0	41.0
34-35	38.31910338881777	40.0	38.0	41.0	34.0	41.0
36-37	38.56645824309233	40.0	38.0	41.0	34.5	41.0
38-39	38.669730937260496	40.0	38.0	41.0	35.0	41.0
40-41	38.65900619314326	40.0	38.0	41.0	35.0	41.0
42-43	38.60257877217698	40.0	38.0	41.0	34.5	41.0
44-45	38.553266992025826	40.0	38.0	41.0	34.0	41.0
46-47	38.48334484871393	40.0	38.0	41.0	34.0	41.0
48-49	38.42362270404564	40.0	38.0	41.0	34.0	41.0
50-51	38.35692814817453	40.0	38.0	41.0	34.0	41.0
52-53	38.249784169137726	40.0	37.5	41.0	34.0	41.0
54-55	38.110459842743225	40.0	37.0	41.0	33.0	41.0
56-57	37.97347234431837	40.0	37.0	41.0	33.0	41.0
58-59	37.811846524036824	40.0	36.5	41.0	33.0	41.0
60-61	37.61993058532309	39.0	36.0	41.0	33.0	41.0
62-63	37.39363342459842	39.0	36.0	40.5	33.0	41.0
64-65	37.11589492425017	39.0	35.0	40.0	32.0	41.0
66-67	36.83764973141682	38.0	35.0	40.0	32.0	41.0
68-69	36.555627927634454	37.5	35.0	40.0	31.5	41.0
70-71	36.24992023809088	37.0	35.0	39.5	31.0	41.0
72-73	35.94804352506493	36.5	35.0	39.0	31.0	40.5
74-75	35.631778867954836	36.0	34.0	39.0	31.0	40.0
76-77	34.58193922210468	35.0	33.5	37.0	29.5	39.0
78-79	34.8593980446339	35.0	34.0	37.0	30.5	39.0
80-81	34.65464072899132	35.0	34.0	37.0	30.0	39.0
82-83	34.407798327634616	35.0	34.0	36.5	30.0	38.5
84-85	34.15064561041521	35.0	34.0	36.0	30.0	37.0
86-87	33.90101549780436	35.0	34.0	36.0	30.0	37.0
88-89	33.703638020689944	35.0	33.5	35.0	30.0	36.5
90-91	33.5346249176743	35.0	33.0	35.0	30.0	36.0
92-93	33.40771546426636	35.0	33.0	35.0	30.0	36.0
94-95	33.332459812429	35.0	33.0	35.0	30.0	36.0
96-97	33.28817681441414	34.0	33.0	35.0	30.0	35.0
98-99	33.30136220791347	34.0	33.0	35.0	30.0	35.0
100-101	32.27215602751079	33.0	31.0	34.5	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	8.0
20	54.0
21	283.0
22	1300.0
23	4157.0
24	10606.0
25	21758.0
26	38641.0
27	59831.0
28	86117.0
29	115241.0
30	147982.0
31	188225.0
32	236833.0
33	308710.0
34	432388.0
35	626798.0
36	1066206.0
37	2167846.0
38	2963670.0
39	1332538.0
40	54799.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.6057225721313	21.872444936334592	15.54238036105264	23.979452130481466
2	13.43514835404806	22.3153953492333	41.79911344835823	22.450342848360414
3	16.481726311388563	27.566276165499342	31.7784353209568	24.173562202155292
4	11.652566557751985	19.40511902384329	40.03036296671023	28.911951451694495
5	12.502454635248553	38.421263766359885	36.283984849540104	12.792296748851454
6	28.344713615411855	39.529293974416646	19.389879816394803	12.736112593776697
7	25.093058174931425	33.517356210077644	24.327404597185865	17.062181017805063
8	23.686897334089622	37.29374081570481	21.810718487507838	17.208643362697735
9	25.190559239042443	17.004077354880522	21.97771227128811	35.82765113478892
10-11	23.32058629806408	27.38514717113039	29.807974316283474	19.48629221452205
12-13	24.41399238624764	25.05388537887243	29.794526415418176	20.737595819461756
14-15	21.558564148585177	26.403767098342072	27.52802470292905	24.5096440501437
16-17	20.922151962996075	29.46378545882911	28.135299591365744	21.478762986809073
18-19	20.93395104169856	28.66442125931647	30.001020885471654	20.40060681351332
20-21	21.82592984187449	27.774663117373144	29.83231423109291	20.567092809659453
22-23	21.621995943778245	27.69543418028398	29.586794841624748	21.095775034313025
24-25	21.29420186243769	27.843943933675654	29.736050288768762	21.125803915117903
26-27	21.259791474023633	27.943739855725763	29.65350453874556	21.14296413150505
28-29	21.19730866565452	28.072785814217255	29.62359312794024	21.106312392187977
30-31	21.450423814550106	27.943392177919037	29.56217467244902	21.044009335081835
32-33	21.298153733416832	28.049551311326393	29.472369331355665	21.17992562390111
34-35	21.446588528490306	28.114098396943156	29.3898613355856	21.049451738980938
36-37	21.406476877429235	28.068965969879013	29.387491253706056	21.137065898985703
38-39	21.310278913051548	28.16768703835828	29.40002556943545	21.122008479154726
40-41	21.48209089301804	28.07392141577345	29.254415488253173	21.18957220295534
42-43	21.415565751217436	28.208605797579946	29.008502143096145	21.367326308106477
44-45	21.542482240723622	28.281970831279157	28.823914572217056	21.35163235578016
46-47	21.578588950410147	28.31116357808731	28.552359001308442	21.5578884701941
48-49	21.568608402862516	28.448805312833684	28.435956357562702	21.546629926741094
50-51	21.593827607519298	28.468316582695664	28.338064611233666	21.599791198551376
52-53	21.670905717511832	28.44486535084347	28.201786453247497	21.682442478397196
54-55	21.708956000192014	28.35340451388183	28.247868378781522	21.689771107144633
56-57	21.669249717589818	28.451190856138137	28.22254448462114	21.657014941650914
58-59	21.649391546408395	28.493884653198364	28.231847002072925	21.624876798320315
60-61	21.753730631279062	28.430886428128645	28.21920878628264	21.596174154309658
62-63	21.731364467998958	28.46599946039044	28.17415844664652	21.62847762496409
64-65	21.70041747127953	28.42587612064739	28.16478769097462	21.70891871709846
66-67	21.719933341014148	28.449031079214805	28.06543336834382	21.765602211427225
68-69	21.68582429576452	28.46427658540026	28.08981349659934	21.760085622235874
70-71	21.70167688641914	28.272759491098874	28.140179874086492	21.885383748395494
72-73	21.737455430239354	28.299537121434902	28.13087298791438	21.83213446041136
74-75	21.668529736652584	28.354304340806813	28.099288747638308	21.877877174902302
76-77	21.745868631043365	28.296034766188882	28.024590126608796	21.93350647615896
78-79	21.676155780718293	28.284528245436235	28.060622010306275	21.978693963539197
80-81	21.766698945351976	28.258692131211056	28.03293369069474	21.941675232742227
82-83	21.77287426599145	28.25553937308247	28.10570488762916	21.865881473296923
84-85	21.867125605673817	28.238363509652064	28.077400575556737	21.817110309117382
86-87	21.719900536491572	28.242896977434473	28.12642166413855	21.9107808219354
88-89	21.801424537905056	28.14734700999148	28.104673433006027	21.94655501909743
90-91	21.802400517830623	28.167839253096044	28.120594724620307	21.909165504453025
92-93	21.824826458139803	28.041687525599603	28.209527200494726	21.92395881576587
94-95	21.626787549539216	28.13758795204088	28.268833470034664	21.966791028385234
96-97	21.639825039025453	28.207714294246767	28.256965114302712	21.895495552425064
98-99	21.887735939083917	28.742394042352448	28.336212406844236	21.033657611719395
100-101	22.441237701660178	31.429876003590845	25.56533755008373	20.56354874466525
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2432.0
1	2119.5
2	2831.0
3	4043.5
4	5376.0
5	8179.0
6	10466.0
7	12562.0
8	14173.0
9	14116.5
10	12869.0
11	11714.5
12	13100.0
13	18075.0
14	29613.5
15	45311.0
16	57943.5
17	64477.0
18	65293.5
19	62297.5
20	57445.5
21	52994.5
22	51733.5
23	56210.5
24	65930.0
25	81212.0
26	100999.5
27	122902.0
28	147224.5
29	173427.0
30	198955.5
31	225601.5
32	252094.5
33	275347.0
34	298027.0
35	318616.0
36	339674.5
37	358960.5
38	371729.0
39	380611.0
40	389298.5
41	397025.0
42	397971.0
43	397403.5
44	398559.0
45	399325.5
46	398031.0
47	393579.0
48	386196.5
49	374995.5
50	362241.5
51	345873.0
52	325140.5
53	304170.0
54	283155.5
55	259662.0
56	235564.5
57	210585.5
58	184547.5
59	157725.5
60	129775.5
61	104493.5
62	81998.5
63	62664.0
64	46879.0
65	34551.0
66	25998.0
67	19740.5
68	14624.5
69	10439.5
70	7446.0
71	5360.0
72	3826.0
73	2678.5
74	1879.0
75	1306.0
76	832.5
77	492.0
78	312.0
79	203.0
80	133.5
81	96.0
82	72.0
83	51.0
84	35.5
85	23.5
86	15.5
87	14.5
88	12.0
89	7.0
90	3.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.0275764591479334E-5
2	0.0015916475204311277
3	2.0275764591479334E-5
4	3.0413646887219E-5
5	2.0275764591479334E-5
6	2.0275764591479334E-5
7	2.0275764591479334E-5
8	8.110305836591734E-5
9	0.0
10-11	0.0
12-13	0.0
14-15	6.234797611879895E-4
16-17	0.0028639517485464557
18-19	7.096517607017767E-5
20-21	0.0
22-23	0.0010797954107072817
24-25	0.005704212111913296
26-27	0.010443418734022304
28-29	0.3632970564022575
30-31	0.331157467252666
32-33	0.33406505027213507
34-35	0.1901152136071507
36-37	0.04785289046679913
38-39	0.038014923266488324
40-41	0.024333466278160815
42-43	0.03146558848690458
44-45	0.020096231306411402
46-47	0.01456212658299992
48-49	0.009794088020139865
50-51	0.005589877656896448
52-53	0.0038708805108253813
54-55	7.359738757834278E-4
56-57	0.0025908266026739876
58-59	0.004800016253078289
60-61	0.0038161985922730082
62-63	0.0030992813924424194
64-65	4.2935400598450793E-4
66-67	7.511321728572381E-5
68-69	7.409741184158323E-4
70-71	9.605418257330805E-4
72-73	0.0017735636529912014
74-75	0.0020014729157739925
76-77	9.532377966436559E-4
78-79	1.4726211201725715E-4
80-81	0.00356157352552097
82-83	0.001449686466078592
84-85	3.808269861111129E-5
86-87	1.9265913467920296E-5
88-89	0.0015672311603532397
90-91	0.004331329999813429
92-93	0.015538048075871714
94-95	0.0016999544956180612
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	142.0
20-21	598.0
22-23	1305.0
24-25	3123.0
26-27	4406.0
28-29	7168.0
30-31	18866.0
32-33	25274.0
34-35	35641.0
36-37	49734.0
38-39	59245.0
40-41	63629.0
42-43	75877.0
44-45	91224.0
46-47	90428.0
48-49	79510.0
50-51	70487.0
52-53	66425.0
54-55	70622.0
56-57	73470.0
58-59	74467.0
60-61	75941.0
62-63	73250.0
64-65	78650.0
66-67	83712.0
68-69	84764.0
70-71	84294.0
72-73	82329.0
74-75	83488.0
76-77	84812.0
78-79	89879.0
80-81	91726.0
82-83	89438.0
84-85	91041.0
86-87	95371.0
88-89	102192.0
90-91	111617.0
92-93	114761.0
94-95	130132.0
96-97	192310.0
98-99	832566.0
100-101	6230079.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.32143442053302
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.61114815693487	73.83207970086184
2	7.651820987833228	12.751214012708093
3	2.0293040793931625	5.0725358031143255
4	0.7822017219500574	2.6069667791635887
5	0.3736431997805059	1.5566243683594772
6	0.1838577701347979	0.9191575882195198
7	0.11234751502499367	0.6552669273825401
8	0.07304987841514124	0.4869296523036081
9	0.04790760155376625	0.35925570729964446
>10	0.13277790816161014	1.6173962656911414
>50	0.0016606414813228765	0.08897482755530785
>100	2.6011078239890383E-4	0.04449193738873118
>500	2.042855420694965E-5	0.009106429952184528
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.0275764591479334E-5
2	0.0	1.0137882295739667E-5	0.0	0.0	3.0413646887218998E-5
3	0.0	1.0137882295739667E-5	0.0	1.0137882295739667E-5	3.0413646887218998E-5
4	0.0	3.0413646887218998E-5	0.0	1.0137882295739667E-5	3.0413646887218998E-5
5	0.0	3.0413646887218998E-5	0.0	2.0275764591479334E-5	3.0413646887218998E-5
6	0.0	3.0413646887218998E-5	0.0	2.0275764591479334E-5	4.055152918295867E-5
7	0.0	3.0413646887218998E-5	0.0	2.0275764591479334E-5	4.055152918295867E-5
8	0.0	3.0413646887218998E-5	0.0	3.0413646887218998E-5	4.055152918295867E-5
9	0.0	8.110305836591734E-5	0.0	4.055152918295867E-5	4.055152918295867E-5
10-11	1.0137882295739667E-5	8.110305836591734E-5	0.0	5.575835262656817E-5	4.055152918295867E-5
12-13	1.52068234436095E-5	8.110305836591734E-5	0.0	6.0827293774437996E-5	4.055152918295867E-5
14-15	2.0275764591479334E-5	1.0644776410526649E-4	0.0	6.589623492230784E-5	4.56204703308285E-5
16-17	3.0413646887219E-5	1.1151670525313633E-4	0.0	8.110305836591734E-5	6.0827293774437996E-5
18-19	4.055152918295867E-5	1.1658564640100616E-4	0.0	8.617199951378717E-5	6.0827293774437996E-5
20-21	4.055152918295867E-5	1.2165458754887599E-4	0.0	1.0644776410526649E-4	6.589623492230784E-5
22-23	4.055152918295867E-5	1.52068234436095E-4	0.0	2.8386070428071066E-4	9.1240940661657E-5
24-25	4.055152918295867E-5	1.7741294017544417E-4	0.0	9.783056415388779E-4	1.0644776410526649E-4
26-27	4.055152918295867E-5	1.82481881323314E-4	0.0	0.0030869851590527284	1.1151670525313633E-4
28-29	4.055152918295867E-5	1.82481881323314E-4	0.0	0.015429856854115772	1.1658564640100616E-4
30-31	4.56204703308285E-5	1.9261976361905367E-4	0.0	0.02159368928992549	1.2165458754887599E-4
32-33	5.068941147869833E-5	1.9261976361905367E-4	0.0	0.023844299159579696	1.4193035214035533E-4
34-35	5.575835262656817E-5	2.1289552821053298E-4	0.0	0.026743733496161243	1.52068234436095E-4
36-37	6.0827293774437996E-5	2.3317129280201233E-4	0.0	0.031097953942181428	1.6220611673183467E-4
38-39	6.0827293774437996E-5	2.4330917509775198E-4	0.0	0.038878778604161626	1.6220611673183467E-4
40-41	6.0827293774437996E-5	2.6358493968923135E-4	0.0	0.054780046985029286	1.7234399902757433E-4
42-43	6.0827293774437996E-5	2.6358493968923135E-4	0.0	0.06238345870683404	1.8755082247118383E-4
44-45	6.0827293774437996E-5	2.889296454285805E-4	0.0	0.07227296288632809	2.1289552821053298E-4
46-47	6.0827293774437996E-5	3.0413646887219E-4	0.0	0.08376932140969687	2.2303341050627267E-4
48-49	6.0827293774437996E-5	3.092054100200598E-4	0.0	0.0949209919350105	2.2303341050627267E-4
50-51	6.0827293774437996E-5	3.5482588035088834E-4	0.0	0.10563673352160732	2.3317129280201233E-4
52-53	6.0827293774437996E-5	3.5482588035088834E-4	0.0	0.1190694275634624	2.6358493968923135E-4
54-55	7.096517607017767E-5	3.5482588035088834E-4	0.0	0.14500719941711232	2.9906752772432016E-4
56-57	7.096517607017767E-5	3.5482588035088834E-4	0.0	0.1715228305616194	3.0413646887219E-4
58-59	7.096517607017767E-5	3.5482588035088834E-4	0.0	0.19892552640700373	3.0413646887219E-4
60-61	7.096517607017767E-5	3.5482588035088834E-4	0.0	0.21708754253982135	3.092054100200598E-4
62-63	7.096517607017767E-5	3.64963762646628E-4	0.0	0.22717980436523016	3.2441223346366935E-4
64-65	7.096517607017767E-5	4.3592893871680565E-4	0.0	0.23704396383898488	3.2441223346366935E-4
66-67	7.096517607017767E-5	4.3592893871680565E-4	0.0	0.24342069180300513	3.2948117461153916E-4
68-69	7.096517607017767E-5	4.4606682101254534E-4	0.0	0.24943752494552662	3.34550115759409E-4
70-71	7.096517607017767E-5	4.4606682101254534E-4	0.0	0.25496267079670476	3.5482588035088834E-4
72-73	7.096517607017767E-5	4.4606682101254534E-4	0.0	0.25917496089058456	3.8523952723810734E-4
74-75	7.096517607017767E-5	4.6634258560402465E-4	0.0	0.262429221107517	3.8523952723810734E-4
76-77	7.096517607017767E-5	4.916872913433739E-4	0.0	0.2656885502655973	3.8523952723810734E-4
78-79	8.110305836591734E-5	4.967562324912437E-4	0.0	0.2690391203643393	3.8523952723810734E-4
80-81	8.110305836591734E-5	4.967562324912437E-4	0.0	0.270412803415412	3.9030846838597716E-4
82-83	8.110305836591734E-5	4.967562324912437E-4	0.0	0.27096024905938193	4.1565317412532634E-4
84-85	8.110305836591734E-5	5.068941147869833E-4	0.0	0.2712947991751413	4.1565317412532634E-4
86-87	8.110305836591734E-5	5.068941147869833E-4	0.0	0.2714164537626902	4.308599975689358E-4
88-89	8.110305836591734E-5	5.271698793784627E-4	0.0	0.2714823499976125	4.3592893871680565E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13130	0.0	50.451378	1
GTATCAA	24275	0.0	37.067074	1
ATCAACG	29945	0.0	29.361408	3
TCAACGC	30095	0.0	29.215063	4
CAACGCA	30540	0.0	28.81743	5
TATCAAC	31550	0.0	28.20755	2
AACGCAG	31450	0.0	28.038097	6
ACGCAGA	35710	0.0	24.669315	7
CGCAGAG	36390	0.0	24.208334	8
GCAGAGT	42110	0.0	20.696253	9
GTACATG	28660	0.0	19.734493	1
TACATGG	28375	0.0	19.41929	2
GAGTACT	25740	0.0	19.386475	12-13
AGAGTAC	38450	0.0	19.330622	10-11
ACATGGG	28125	0.0	18.967089	3
CAGAGTA	40990	0.0	18.587532	10-11
AGTACTT	28420	0.0	17.528177	12-13
CATGGGG	20765	0.0	17.436071	4
GTGGTAT	6820	0.0	17.277327	1
GTACTTT	29060	0.0	17.179491	14-15
>>END_MODULE