FastQCFastQC Report
Fri 27 May 2016
SRR2139028_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139028_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8944573
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA134950.050.3603821
GTATCAA249550.036.2547761
TCAACGC307800.028.648784
ATCAACG308250.028.6482163
TATCAAC315750.028.0762482
CAACGCA314550.028.020525
AACGCAG323050.027.30956
CGCAGAG374700.023.5563748
ACGCAGA375600.023.5112157
ACCGAAC13800.021.1973428
GCAGAGT426100.020.6554439
GTACATG305350.020.4657631
TACATGG305850.020.1134852
GGACCGA15150.019.3084726
GAGTACT251950.019.29334412-13
ACATGGG310150.019.2870883
CCGAACC14850.018.8423989
AGAGTAC401250.018.62880910-11
CAGAGTA422200.018.3320710-11
CATGGGG213850.017.901524
Produced by FastQC (version 0.11.5)