##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139028_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8944573
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.68615013818994	34.0	31.0	34.0	31.0	34.0
2	32.7891256519456	34.0	31.0	34.0	31.0	34.0
3	32.856434287025216	34.0	31.0	34.0	31.0	34.0
4	36.31025908112103	37.0	37.0	37.0	35.0	37.0
5	36.25435624484254	37.0	37.0	37.0	35.0	37.0
6	36.29507970922704	37.0	37.0	37.0	35.0	37.0
7	36.24456852216422	37.0	37.0	37.0	35.0	37.0
8	36.22563011112995	37.0	37.0	37.0	35.0	37.0
9	37.95727096195648	39.0	38.0	39.0	35.0	39.0
10-11	37.91690173471668	39.0	38.0	39.0	35.0	39.0
12-13	37.89117367592617	39.0	38.0	39.0	35.0	39.0
14-15	39.290106749645844	41.0	39.0	41.0	36.0	41.0
16-17	39.26529304417326	41.0	39.0	41.0	36.0	41.0
18-19	39.290370373186065	41.0	39.0	41.0	36.0	41.0
20-21	39.28872188933832	41.0	39.0	41.0	36.0	41.0
22-23	39.15706792130108	40.5	39.0	41.0	36.0	41.0
24-25	39.10545028721213	40.0	39.0	41.0	35.0	41.0
26-27	39.051093612260665	40.0	39.0	41.0	35.0	41.0
28-29	38.86482331206088	40.0	39.0	41.0	35.0	41.0
30-31	38.75144273819549	40.0	38.0	41.0	35.0	41.0
32-33	38.63755236426593	40.0	38.0	41.0	35.0	41.0
34-35	38.35798578974039	40.0	38.0	41.0	34.0	41.0
36-37	38.60134587638248	40.0	38.0	41.0	34.5	41.0
38-39	38.70433046681792	40.0	38.0	41.0	35.0	41.0
40-41	38.69604659620266	40.0	38.0	41.0	35.0	41.0
42-43	38.642934308261374	40.0	38.0	41.0	35.0	41.0
44-45	38.59283254745456	40.0	38.0	41.0	34.5	41.0
46-47	38.52581802477884	40.0	38.0	41.0	34.0	41.0
48-49	38.46665293113771	40.0	38.0	41.0	34.0	41.0
50-51	38.39797229629424	40.0	38.0	41.0	34.0	41.0
52-53	38.29669071621602	40.0	38.0	41.0	34.0	41.0
54-55	38.15933662171474	40.0	37.0	41.0	34.0	41.0
56-57	38.02332671383114	40.0	37.0	41.0	33.0	41.0
58-59	37.86344958237528	40.0	36.5	41.0	33.0	41.0
60-61	37.67554951284555	39.0	36.0	41.0	33.0	41.0
62-63	37.443803993999424	39.0	36.0	40.5	33.0	41.0
64-65	37.16503714679881	39.0	35.0	40.0	32.0	41.0
66-67	36.88797754477443	38.0	35.0	40.0	32.0	41.0
68-69	36.60065612422835	37.5	35.0	40.0	32.0	41.0
70-71	36.2959423116134	37.0	35.0	39.5	31.5	41.0
72-73	35.99461981020823	36.5	35.0	39.0	31.0	40.5
74-75	35.67428487567809	36.0	34.5	39.0	31.0	40.0
76-77	34.62735219517285	35.0	33.5	37.0	30.0	39.0
78-79	34.90454439828929	35.0	34.0	37.0	31.0	39.0
80-81	34.69982904773216	35.0	34.0	37.0	31.0	39.0
82-83	34.45770170205547	35.0	34.0	36.5	31.0	38.5
84-85	34.203646463671674	35.0	34.0	36.0	30.0	37.0
86-87	33.96199571460129	35.0	34.0	36.0	30.0	37.0
88-89	33.76793519547112	35.0	34.0	35.0	30.0	36.5
90-91	33.60139030516852	35.0	33.0	35.0	30.0	36.0
92-93	33.474744159729724	35.0	33.0	35.0	30.0	36.0
94-95	33.400255306583716	35.0	33.0	35.0	30.0	36.0
96-97	33.35283234119544	34.5	33.0	35.0	30.0	35.0
98-99	33.36281337210271	34.0	33.0	35.0	30.0	35.0
100-101	32.32824133382158	33.0	31.0	34.5	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	0.0
13	0.0
14	1.0
15	0.0
16	0.0
17	1.0
18	3.0
19	16.0
20	51.0
21	268.0
22	1225.0
23	3943.0
24	9595.0
25	19615.0
26	33836.0
27	52754.0
28	74803.0
29	100404.0
30	128113.0
31	161956.0
32	205591.0
33	270337.0
34	381985.0
35	549619.0
36	943939.0
37	1958070.0
38	2706189.0
39	1284834.0
40	57423.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.05220272721856	21.690833467586092	15.371748963700998	23.885214841494353
2	13.531600906979788	22.195637029649017	41.712796963377464	22.55996509999373
3	16.48109227373789	27.762326443604728	31.740024200154487	24.016557082502896
4	11.678213709546686	19.273626345369312	40.07481634108739	28.973343603996614
5	12.39948791283562	38.64455880555926	36.16344484268726	12.792508438917865
6	28.215682091887683	39.54416153170884	19.34493903118003	12.89521734522345
7	25.145261282485066	33.60682881193197	24.276253799511032	16.97165610607193
8	24.021785746068787	36.85571482299193	21.752273224273424	17.37022620666586
9	25.556625229622476	16.790248120284783	21.945429927174835	35.70769672291791
10-11	23.40420833951492	27.358499952988254	29.680947318558417	19.55634438893841
12-13	24.50409589189958	25.171802518890786	29.65200570804282	20.672095881166815
14-15	21.71929437182854	26.325554355254127	27.623064979785088	24.332086293132242
16-17	20.814179869701572	29.437004863481135	28.21790627083901	21.530908995978283
18-19	20.85889097289702	28.60214233899127	30.20047527161592	20.338491416495785
20-21	21.825132844916027	27.621931776611248	30.044814196003163	20.50812118246957
22-23	21.604190943234496	27.596231648647844	29.768872072478636	21.030705335639023
24-25	21.330124564607733	27.765574882212977	29.929228798251913	20.97507175492738
26-27	21.21480458246135	27.866307239501566	29.846853275897207	21.072034902139876
28-29	21.074849699263698	28.003486680458185	29.889448005181034	21.032215615097083
30-31	21.3932085485994	27.855545428695404	29.820251887231198	20.930994135474
32-33	21.202221976846488	27.99024479273144	29.7062553733481	21.10127785707397
34-35	21.31721051456654	28.034708440191036	29.674206289125905	20.97387475611652
36-37	21.351916395256723	27.912216084634466	29.685729862028058	21.050137658080747
38-39	21.198867583163036	28.148859611969	29.60439337332439	21.047879431543574
40-41	21.36864381392857	28.057409372620555	29.492121459201755	21.08182535424912
42-43	21.352898453781258	28.142834147679096	29.202440879897146	21.3018265186425
44-45	21.455332427540515	28.2463905931133	29.02709829206616	21.27117868728002
46-47	21.476343356140855	28.2437735025306	28.699345466531817	21.580537674796727
48-49	21.473177905761172	28.46005808999237	28.520565019240657	21.546198985005805
50-51	21.454978646919418	28.421631142013247	28.453459988905962	21.66993022216138
52-53	21.57409879629046	28.411179174442026	28.31547080685341	21.699251222414105
54-55	21.612666795809766	28.35006226105084	28.374746342450162	21.66252460068923
56-57	21.626931299625472	28.39156788087812	28.294891756330475	21.686609063165935
58-59	21.528710214432113	28.513151292896204	28.34993004803792	21.608208444633764
60-61	21.718276408344085	28.516850347292817	28.250672911474652	21.514200332888446
62-63	21.634118097885985	28.507244366159373	28.22755942281669	21.63107811313795
64-65	21.578765211902496	28.421308453097144	28.273318671649665	21.726607663350695
66-67	21.745317740381704	28.457251038273373	28.046930525884235	21.75050069546069
68-69	21.574855004530445	28.459835347536245	28.18649800094762	21.778811646985694
70-71	21.63528775997196	28.256512761072727	28.267421299522947	21.84077817943237
72-73	21.7022383228802	28.30265949513853	28.155344087418232	21.839758094563035
74-75	21.575442292356005	28.39205170487332	28.118691957908204	21.91381404486247
76-77	21.663612690755134	28.319971363598313	28.110381255557627	21.906034690088934
78-79	21.668310427623968	28.26316825893562	28.09137630208972	21.97714501135069
80-81	21.65823665450434	28.301524826966677	28.060457264218396	21.97978125431058
82-83	21.68038592132048	28.264318762053055	28.179632878733287	21.875662437893183
84-85	21.78636424824249	28.232308965642932	28.088690351117318	21.892636434997264
86-87	21.601123954219894	28.25093004991418	28.193304395222306	21.95464160064362
88-89	21.697363126843243	28.15501542624815	28.18720569019728	21.960415756711324
90-91	21.716139800948437	28.212927047381914	28.208353121965253	21.8625800297044
92-93	21.824427788706778	28.115826497781004	28.181953778054265	21.877791935457953
94-95	21.57962142104609	28.119890655451634	28.268387437597564	22.03210048590471
96-97	21.59440623377015	28.171931068090196	28.316246139789804	21.917416558349853
98-99	21.81581463258318	28.703639842068014	28.359154308215835	21.121391217132974
100-101	22.321175590140385	31.42217188333451	25.592754417438474	20.663898109086627
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2485.0
1	2327.0
2	3321.5
3	4706.0
4	6197.5
5	9254.0
6	11770.0
7	14344.0
8	16784.0
9	17244.0
10	15787.0
11	13909.5
12	14714.0
13	19299.5
14	30809.0
15	47019.0
16	59765.0
17	66021.5
18	66204.0
19	61868.0
20	55677.5
21	49737.0
22	46820.0
23	49902.0
24	58987.5
25	73393.0
26	91936.5
27	112665.5
28	134609.5
29	157788.5
30	179987.0
31	201814.5
32	227807.5
33	255420.0
34	279545.0
35	300056.0
36	322360.0
37	340101.0
38	346116.0
39	346444.0
40	350513.0
41	356886.5
42	358381.0
43	361665.0
44	365374.0
45	363684.5
46	360117.5
47	353516.5
48	345344.5
49	337744.0
50	325264.0
51	308502.5
52	290596.0
53	273522.5
54	256982.5
55	237118.0
56	215801.0
57	193059.5
58	170089.5
59	148687.5
60	126225.5
61	102923.0
62	79898.0
63	60017.5
64	44711.5
65	33647.0
66	25736.5
67	19843.0
68	15163.5
69	10720.5
70	7183.0
71	5120.0
72	3649.5
73	2490.5
74	1718.5
75	1179.0
76	730.5
77	482.5
78	332.5
79	208.0
80	131.0
81	87.0
82	61.0
83	43.5
84	30.5
85	24.0
86	20.5
87	19.5
88	13.5
89	6.0
90	3.5
91	2.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.2359927075333836E-5
2	0.0018111540931020408
3	2.2359927075333836E-5
4	3.353989061300075E-5
5	2.2359927075333836E-5
6	1.1179963537666918E-5
7	1.1179963537666918E-5
8	1.0061967183900226E-4
9	0.0
10-11	0.0
12-13	1.1179963537666918E-5
14-15	6.763877940288486E-4
16-17	0.0029012005380245655
18-19	3.9129872381834216E-5
20-21	0.0
22-23	0.0011740389135756972
24-25	0.005782339326461484
26-27	0.010484493244271462
28-29	0.35977328103469974
30-31	0.32896219061682386
32-33	0.3316529531275324
34-35	0.19046834233189908
36-37	0.05146533221837382
38-39	0.0418020756892756
40-41	0.026511869325457377
42-43	0.03402095480353159
44-45	0.02264844758609285
46-47	0.017410895903912628
48-49	0.012679556475555652
50-51	0.008102545234595773
52-53	0.0061919752728930985
54-55	0.0016096724298244026
56-57	0.004445533325693602
58-59	0.007489396149054685
60-61	0.006175166586437712
62-63	0.005514824756448141
64-65	0.0013579798128614139
66-67	7.764720680997062E-5
68-69	6.801342401877065E-4
70-71	8.462309731907418E-4
72-73	0.0015501721225598154
74-75	0.0017086802781623432
76-77	8.740526941786587E-4
78-79	1.1051255515871571E-4
80-81	0.0035165082233859406
82-83	0.0013730744488751653
84-85	5.014864057065141E-5
86-87	1.4511620833405288E-5
88-89	0.0016694237899214752
90-91	0.0037753978512017217
92-93	0.015868028922551743
94-95	0.001648909681588606
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	196.0
20-21	605.0
22-23	1390.0
24-25	4328.0
26-27	4559.0
28-29	7460.0
30-31	19905.0
32-33	26462.0
34-35	37603.0
36-37	50702.0
38-39	60687.0
40-41	64860.0
42-43	77562.0
44-45	92848.0
46-47	91198.0
48-49	80109.0
50-51	71402.0
52-53	65967.0
54-55	70246.0
56-57	73311.0
58-59	73470.0
60-61	73216.0
62-63	71914.0
64-65	76941.0
66-67	81545.0
68-69	82603.0
70-71	81006.0
72-73	78903.0
74-75	81214.0
76-77	81933.0
78-79	86429.0
80-81	88035.0
82-83	85394.0
84-85	87151.0
86-87	91272.0
88-89	95581.0
90-91	102964.0
92-93	105254.0
94-95	118067.0
96-97	169861.0
98-99	732262.0
100-101	5498158.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.24263635335889
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.91039476856707	74.0113565975341
2	7.448531997611324	12.400708808870347
3	1.9734451335272514	4.928243268405443
4	0.7452068020604494	2.4813191532786996
5	0.34370102714904455	1.4305289808621924
6	0.17928340026227294	0.8954413735335645
7	0.11210005161929988	0.6532052682496697
8	0.07006274483538857	0.466576607220034
9	0.05016326098916726	0.37581498835378957
>10	0.16430061477488442	2.073941447497148
>50	0.0022313049207110364	0.11968166104809337
>100	4.38369351766908E-4	0.07546945773091666
>500	1.171038285162412E-4	0.06529216678439065
>1k	2.342050285947662E-5	0.022420220631558466
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.2359927075333836E-5
2	0.0	2.2359927075333836E-5	0.0	0.0	3.353989061300075E-5
3	0.0	3.353989061300075E-5	0.0	1.1179963537666918E-5	3.353989061300075E-5
4	0.0	1.0061967183900227E-4	0.0	1.1179963537666918E-5	3.353989061300075E-5
5	1.1179963537666918E-5	1.1179963537666918E-4	0.0	1.1179963537666918E-5	3.353989061300075E-5
6	1.1179963537666918E-5	1.1179963537666918E-4	0.0	1.1179963537666918E-5	4.471985415066767E-5
7	1.1179963537666918E-5	1.1179963537666918E-4	0.0	1.1179963537666918E-5	4.471985415066767E-5
8	2.2359927075333836E-5	1.229795989143361E-4	0.0	1.1179963537666918E-5	4.471985415066767E-5
9	2.2359927075333836E-5	2.1241930721567145E-4	0.0	2.2359927075333836E-5	4.471985415066767E-5
10-11	7.825974476366843E-5	2.1241930721567145E-4	0.0	3.353989061300075E-5	4.471985415066767E-5
12-13	8.384972653250189E-5	2.1241930721567145E-4	0.0	5.0309835919501135E-5	5.0309835919501135E-5
14-15	8.943970830133534E-5	2.8508907021050644E-4	0.0	5.589981768833459E-5	6.70797812260015E-5
16-17	1.0061967183900227E-4	3.0744899728584027E-4	0.0	6.148979945716805E-5	7.825974476366843E-5
18-19	1.0061967183900227E-4	3.298089243611741E-4	0.0	7.825974476366843E-5	8.384972653250189E-5
20-21	1.1179963537666918E-4	3.577588332053414E-4	0.0	1.0061967183900227E-4	8.943970830133534E-5
22-23	1.34159562452003E-4	4.13658650893676E-4	0.0	3.4657886966767446E-4	8.943970830133534E-5
24-25	1.4533952598966995E-4	4.416085597378433E-4	0.0	0.0013583655698265306	9.502969007016881E-5
26-27	1.6210947129617032E-4	4.4719854150667674E-4	0.0	0.004142176490705593	1.0061967183900227E-4
28-29	1.6769945306500378E-4	4.4719854150667674E-4	0.0	0.019816485370514614	1.0061967183900227E-4
30-31	1.6769945306500378E-4	5.47818213345679E-4	0.0	0.027346190813133282	1.0061967183900227E-4
32-33	1.6769945306500378E-4	5.589981768833459E-4	0.0	0.029626903374817333	1.0061967183900227E-4
34-35	1.6769945306500378E-4	6.484378851846812E-4	0.0	0.0326454935299874	1.1179963537666918E-4
36-37	1.6769945306500378E-4	7.546475387925169E-4	0.0	0.03742492794234001	1.1179963537666918E-4
38-39	1.6769945306500378E-4	7.714174840990173E-4	0.0	0.04608939968403187	1.1179963537666918E-4
40-41	1.6769945306500378E-4	8.161373382496851E-4	0.0	0.06315002404251159	1.1179963537666918E-4
42-43	1.788794166026707E-4	8.440872470938524E-4	0.0	0.0714567369509981	1.1179963537666918E-4
44-45	1.900593801403376E-4	9.61476864239355E-4	0.0	0.08282116988703653	1.1179963537666918E-4
46-47	1.900593801403376E-4	0.0010620965360783573	0.0	0.09584582740841849	1.1179963537666918E-4
48-49	2.3477923429100528E-4	0.0010676865178471907	0.0	0.10756242919589343	1.4533952598966995E-4
50-51	2.3477923429100528E-4	0.0012521559162186947	0.0	0.11929021094690602	1.4533952598966995E-4
52-53	2.3477923429100528E-4	0.0012633358797563617	0.0	0.13436080179568102	1.509295077585034E-4
54-55	2.4036921605983874E-4	0.001268925861525195	0.0	0.1618579221165728	1.5651948952733686E-4
56-57	2.459591978286722E-4	0.0012856958068316955	0.0	0.18971280127066995	1.5651948952733686E-4
58-59	2.459591978286722E-4	0.0013136457156758628	0.0	0.21993783269475245	1.5651948952733686E-4
60-61	2.459591978286722E-4	0.0013248256792135298	0.0	0.23842949238605354	1.5651948952733686E-4
62-63	2.459591978286722E-4	0.0013415956245200302	0.0	0.24981628524916727	1.5651948952733686E-4
64-65	2.459591978286722E-4	0.0015428349681980346	0.0	0.260532280300021	1.5651948952733686E-4
66-67	2.459591978286722E-4	0.001559604913504535	0.0	0.26710609886016917	1.5651948952733686E-4
68-69	2.459591978286722E-4	0.0015819648405798689	0.0	0.273288618696499	1.5651948952733686E-4
70-71	2.459591978286722E-4	0.0015875548223487023	0.0	0.2786158713221973	1.6769945306500378E-4
72-73	2.459591978286722E-4	0.0015987347858863693	0.0	0.28297046712011853	1.900593801403376E-4
74-75	2.459591978286722E-4	0.0016155047311928697	0.0	0.28673811483231226	1.900593801403376E-4
76-77	2.5154917959750565E-4	0.0016714045488812044	0.0	0.290036204075924	1.900593801403376E-4
78-79	2.6272914313517256E-4	0.001704944439494205	0.0	0.29303243430401876	1.900593801403376E-4
80-81	2.68319124904006E-4	0.0017105344212630384	0.0	0.2942286904025491	2.0123934367800454E-4
82-83	2.906790519793399E-4	0.001716124403031872	0.0	0.2947317887617441	2.2918925252217182E-4
84-85	2.906790519793399E-4	0.0017273043665695389	0.0	0.29497774795957277	2.3477923429100528E-4
86-87	2.9626903374817336E-4	0.001755254275413706	0.0	0.2950783676314118	2.3477923429100528E-4
88-89	3.018590155170068E-4	0.0018446939837150416	0.0	0.2951174975037936	2.3477923429100528E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13495	0.0	50.360382	1
GTATCAA	24955	0.0	36.254776	1
TCAACGC	30780	0.0	28.64878	4
ATCAACG	30825	0.0	28.648216	3
TATCAAC	31575	0.0	28.076248	2
CAACGCA	31455	0.0	28.02052	5
AACGCAG	32305	0.0	27.3095	6
CGCAGAG	37470	0.0	23.556374	8
ACGCAGA	37560	0.0	23.511215	7
ACCGAAC	1380	0.0	21.197342	8
GCAGAGT	42610	0.0	20.655443	9
GTACATG	30535	0.0	20.465763	1
TACATGG	30585	0.0	20.113485	2
GGACCGA	1515	0.0	19.308472	6
GAGTACT	25195	0.0	19.293344	12-13
ACATGGG	31015	0.0	19.287088	3
CCGAACC	1485	0.0	18.842398	9
AGAGTAC	40125	0.0	18.628809	10-11
CAGAGTA	42220	0.0	18.33207	10-11
CATGGGG	21385	0.0	17.90152	4
>>END_MODULE