FastQCFastQC Report
Fri 27 May 2016
SRR2139027_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139027_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8080511
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA106550.048.7238961
GTATCAA197950.038.672211
ATCAACG247250.030.5473063
TCAACGC247800.030.4623054
CAACGCA252800.029.8935285
TATCAAC257150.029.4874742
AACGCAG258800.029.2663566
ACGCAGA296150.025.6041137
CGCAGAG303350.024.9682988
GCAGAGT348600.021.605429
GTACATG246100.021.077891
TACATGG242950.020.544152
ACATGGG248050.019.6920473
AGAGTAC319700.019.52650510-11
GAGTACT211350.019.39209712-13
CAGAGTA343250.018.31720510-11
AGTACTT230200.017.84120412-13
GTGGTAT52450.017.3906761
GTACTTT236000.017.34021414-15
CATGGGG178200.017.1736354
Produced by FastQC (version 0.11.5)