##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139027_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8080511
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.68894751829433	34.0	31.0	34.0	31.0	34.0
2	32.79094775070537	34.0	31.0	34.0	31.0	34.0
3	32.85937424006972	34.0	31.0	34.0	31.0	34.0
4	36.31113453097211	37.0	37.0	37.0	35.0	37.0
5	36.25497087993569	37.0	37.0	37.0	35.0	37.0
6	36.29286056290252	37.0	37.0	37.0	35.0	37.0
7	36.24319167438792	37.0	37.0	37.0	35.0	37.0
8	36.22295854804232	37.0	37.0	37.0	35.0	37.0
9	37.95859061388568	39.0	38.0	39.0	35.0	39.0
10-11	37.91760737656319	39.0	38.0	39.0	35.0	39.0
12-13	37.89309735485788	39.0	38.0	39.0	35.0	39.0
14-15	39.301210777387716	41.0	39.0	41.0	36.0	41.0
16-17	39.27332807293994	41.0	39.0	41.0	36.0	41.0
18-19	39.286937732032044	41.0	39.0	41.0	36.0	41.0
20-21	39.28240291733285	41.0	39.0	41.0	36.0	41.0
22-23	39.15336828772497	40.5	39.0	41.0	36.0	41.0
24-25	39.101550538507894	40.0	39.0	41.0	35.5	41.0
26-27	39.05135719292012	40.0	39.0	41.0	35.0	41.0
28-29	38.8636075955334	40.0	39.0	41.0	35.0	41.0
30-31	38.75344476781583	40.0	38.0	41.0	35.0	41.0
32-33	38.63981325704515	40.0	38.0	41.0	35.0	41.0
34-35	38.362153054447504	40.0	38.0	41.0	34.0	41.0
36-37	38.60646295829167	40.0	38.0	41.0	34.5	41.0
38-39	38.70751675889997	40.0	38.0	41.0	35.0	41.0
40-41	38.69853119468706	40.0	38.0	41.0	35.0	41.0
42-43	38.64312350329938	40.0	38.0	41.0	35.0	41.0
44-45	38.59242111906196	40.0	38.0	41.0	34.5	41.0
46-47	38.521352197582736	40.0	38.0	41.0	34.0	41.0
48-49	38.459275198111285	40.0	38.0	41.0	34.0	41.0
50-51	38.38804705070872	40.0	38.0	41.0	34.0	41.0
52-53	38.278813769785515	40.0	38.0	41.0	34.0	41.0
54-55	38.136689880679924	40.0	37.0	41.0	33.5	41.0
56-57	37.99523825882802	40.0	37.0	41.0	33.0	41.0
58-59	37.82748324127262	40.0	36.5	41.0	33.0	41.0
60-61	37.63025400468613	39.0	36.0	41.0	33.0	41.0
62-63	37.394868443764416	39.0	35.5	40.5	33.0	41.0
64-65	37.110590817920375	38.5	35.0	40.0	32.0	41.0
66-67	36.8231287696122	38.0	35.0	40.0	32.0	41.0
68-69	36.533022071249036	37.5	35.0	40.0	32.0	41.0
70-71	36.22305624790913	37.0	35.0	39.5	31.0	41.0
72-73	35.9177651666512	36.5	35.0	39.0	31.0	40.5
74-75	35.598480024044584	36.0	34.0	39.0	31.0	40.0
76-77	34.55413438709894	35.0	33.5	37.0	29.5	39.0
78-79	34.82881122629763	35.0	34.0	37.0	31.0	39.0
80-81	34.62804657070083	35.0	34.0	37.0	31.0	39.0
82-83	34.39078163965513	35.0	34.0	36.0	30.5	38.5
84-85	34.14303037526366	35.0	34.0	36.0	30.0	37.0
86-87	33.90347421714941	35.0	34.0	35.5	30.0	37.0
88-89	33.715706485996165	35.0	34.0	35.0	30.0	36.5
90-91	33.55715401215634	35.0	33.0	35.0	30.0	36.0
92-93	33.43501439204697	35.0	33.0	35.0	30.0	36.0
94-95	33.36418054552467	35.0	33.0	35.0	30.0	35.5
96-97	33.319316367830794	34.0	33.0	35.0	30.0	35.0
98-99	33.33465465299379	34.0	33.0	35.0	30.0	35.0
100-101	32.30389402649059	33.0	31.0	34.5	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	1.0
19	7.0
20	41.0
21	229.0
22	1112.0
23	3655.0
24	8982.0
25	18002.0
26	31688.0
27	48456.0
28	69126.0
29	92100.0
30	117655.0
31	148736.0
32	188376.0
33	245501.0
34	345269.0
35	504147.0
36	866544.0
37	1797458.0
38	2433164.0
39	1112051.0
40	48209.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.23437248816875	21.31582305025587	15.288628476250693	24.161175985324686
2	13.69442646874462	21.947556004050814	41.52233200702989	22.835685520174675
3	16.65680961434484	27.176394457329362	31.646385147272284	24.52041078105352
4	11.86878202845885	18.995164784792642	39.57323727997828	29.56281590677023
5	12.695821513223981	38.26126547226171	36.039338610971164	13.003574403543144
6	28.88570509605274	38.99722158591742	19.120565301022495	12.996508017007343
7	25.458111611533386	33.39672042936899	23.924136462195637	17.22103149690199
8	24.106732477759426	36.79371652899783	21.538624704257238	17.560926288985513
9	25.452251720219177	16.78054766585925	21.671277967445377	36.09592264647619
10-11	23.55922787556381	27.065540780774878	29.577993272950188	19.797238070711124
12-13	24.616135043934722	24.823194968734033	29.537723542483885	21.022946444847364
14-15	21.777768482372778	26.11759163096736	27.333154218029282	24.771485668630582
16-17	21.15540455916474	29.20818558629112	27.914791518878374	21.721618335665767
18-19	21.155801238709405	28.344452891384165	29.813878015327504	20.685867854578927
20-21	22.036918847814732	27.464811479527334	29.66913948221735	20.829130190440583
22-23	21.85317804441489	27.4540364121856	29.386997656605985	21.305787886793528
24-25	21.51875251505816	27.557126677471448	29.53837815493755	21.38574265253284
26-27	21.47814758242241	27.698698070974757	29.4740670914309	21.349087255171934
28-29	21.377146063101392	27.833303159048654	29.487285020656124	21.30226575719383
30-31	21.680780936865133	27.650844376567242	29.37685129430918	21.291523392258444
32-33	21.4678608199315	27.79561376992782	29.281904641570357	21.454620768570326
34-35	21.65430899932381	27.90425589867046	29.224650497746786	21.216784604258947
36-37	21.60671252464062	27.805515488658113	29.186023050963005	21.401748935738265
38-39	21.494242449889704	27.957567541661188	29.184506351073647	21.36368365737546
40-41	21.696845158276137	27.8556498903421	29.003375933462156	21.444129017919604
42-43	21.611470691387265	27.969876828731344	28.80417874884295	21.61447373103844
44-45	21.748021679200153	28.009723578789046	28.594116774604373	21.648137967406427
46-47	21.767527559213047	27.987824005248203	28.332474934907047	21.912173500631702
48-49	21.76935525127847	28.15424888690743	28.25143694316603	21.824958918648075
50-51	21.793523243842046	28.287464562652907	28.083763034208285	21.835249159296758
52-53	21.91968562976329	28.21642274566158	27.935528751624144	21.928362872950988
54-55	21.923772570212353	28.12397947891482	27.993366255016493	21.958881695856334
56-57	21.903060409076527	28.17762473376757	27.989434146680885	21.929880710475018
58-59	21.87243316961598	28.228792013249105	28.01927456348075	21.879500253654165
60-61	21.99345468501752	28.15362300299605	28.02878140618814	21.82414090579829
62-63	21.960219416342706	28.26130938212204	27.92516520827223	21.853305993263024
64-65	21.893505909749315	28.191173480164906	27.921118690019934	21.994201920065837
66-67	21.93270456442896	28.22623853291868	27.80936295816972	22.031693944482644
68-69	21.916070851251124	28.208358473653544	27.860578259201645	22.01499241589369
70-71	21.90497972774311	28.068397425050406	27.879744548375214	22.146878298831265
72-73	21.9468368657263	28.082001713378908	27.833206192358123	22.137955228536665
74-75	21.877242958194557	28.139630383451504	27.803837551940337	22.179289106413602
76-77	21.976212169435897	28.03754589639862	27.79153251679188	22.194709417373605
78-79	21.949159571524664	28.036771735109582	27.789445770673787	22.22462292269196
80-81	21.991943630679433	28.069744287025095	27.71388749486572	22.224424587429752
82-83	22.007071602845002	28.02110232970612	27.85540521195287	22.116420855496
84-85	22.12274245433965	27.957235412533198	27.777811304902244	22.142210828224908
86-87	21.929777588438455	28.009584906588415	27.881709464361432	22.178928040611698
88-89	22.01239810901913	27.898453812904773	27.878971759059073	22.210176319017023
90-91	22.033686786330144	27.913203562271484	27.883337279910403	22.16977237148797
92-93	22.105261502568982	27.765283746836467	27.97440932597287	22.15504542462168
94-95	21.935757546597344	27.818918001826663	28.003838719928726	22.241485731647266
96-97	21.90819804083412	27.89111919277969	28.034893328576587	22.165789437809604
98-99	22.129611972175738	28.446550500729234	28.07582123579809	21.348016291296933
100-101	22.657374674465018	31.140864031770555	25.292050956073375	20.909710337691052
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2225.0
1	1944.5
2	2451.5
3	3438.0
4	4658.0
5	7195.5
6	9289.0
7	10995.5
8	12218.5
9	12278.0
10	11443.5
11	10650.0
12	11826.0
13	15843.0
14	25681.5
15	39011.5
16	49469.0
17	55136.0
18	55714.5
19	52404.0
20	47813.0
21	43936.0
22	42468.5
23	45166.5
24	52194.0
25	63919.5
26	79327.5
27	96307.0
28	116380.0
29	137238.0
30	154915.5
31	173202.0
32	192567.5
33	211325.5
34	229987.5
35	245861.5
36	261588.5
37	277229.0
38	289704.5
39	299280.5
40	306485.5
41	314030.0
42	319983.0
43	324689.5
44	328505.0
45	331133.0
46	330908.5
47	327136.0
48	323590.0
49	318732.0
50	310891.5
51	299503.5
52	283587.5
53	265483.5
54	247547.0
55	227822.0
56	207602.0
57	186639.0
58	165152.0
59	142568.5
60	118092.5
61	95290.0
62	74940.0
63	57404.0
64	42870.5
65	32085.0
66	24314.5
67	18271.0
68	13900.0
69	10307.0
70	7414.5
71	5388.5
72	3849.5
73	2665.5
74	1756.0
75	1115.5
76	686.0
77	438.5
78	292.0
79	196.5
80	135.0
81	88.0
82	63.0
83	46.5
84	29.5
85	18.5
86	12.5
87	9.5
88	10.0
89	8.5
90	4.0
91	2.0
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	1.5
98	1.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.475090993626517E-5
2	0.0019553218849649484
3	2.475090993626517E-5
4	2.475090993626517E-5
5	2.475090993626517E-5
6	2.475090993626517E-5
7	2.475090993626517E-5
8	1.1137909471319327E-4
9	0.0
10-11	0.0
12-13	0.0
14-15	6.806500232472921E-4
16-17	0.0032052428367463394
18-19	4.950181987253034E-5
20-21	0.0
22-23	0.0010149014137762348
24-25	0.0055644644756912744
26-27	0.010092462435142772
28-29	0.36211210316729
30-31	0.33110399814837776
32-33	0.33399807662855063
34-35	0.1912643656373599
36-37	0.05189849738600539
38-39	0.0413744923860666
40-41	0.02779206412605055
42-43	0.03581279393700321
44-45	0.024281778439211362
46-47	0.018874907090402144
48-49	0.013478258582692075
50-51	0.008223792745566928
52-53	0.0063303768178525986
54-55	0.0015630761527332913
56-57	0.004912623587646199
58-59	0.008176859227931769
60-61	0.0066935454929101315
62-63	0.005929593541012348
64-65	0.001569611200970766
66-67	9.950814545247775E-5
68-69	7.899300294342291E-4
70-71	0.0010811559952095355
72-73	0.0018401805400394694
74-75	0.0021480087662459828
76-77	0.0010853633807826758
78-79	1.2865383513677E-4
80-81	0.003391476354825902
82-83	0.0016267334587932148
84-85	8.621253144602085E-5
86-87	2.379962996335333E-5
88-89	0.001462740072214352
90-91	0.0039532135142845495
92-93	0.01537561179708297
94-95	0.0018841639552369747
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	145.0
20-21	551.0
22-23	1124.0
24-25	2614.0
26-27	3626.0
28-29	6458.0
30-31	16855.0
32-33	22370.0
34-35	32760.0
36-37	43735.0
38-39	51604.0
40-41	55105.0
42-43	65609.0
44-45	78587.0
46-47	76980.0
48-49	67859.0
50-51	60540.0
52-53	57537.0
54-55	61846.0
56-57	63760.0
58-59	63636.0
60-61	64421.0
62-63	62686.0
64-65	67576.0
66-67	71945.0
68-69	71838.0
70-71	71576.0
72-73	69329.0
74-75	70308.0
76-77	72012.0
78-79	75252.0
80-81	76954.0
82-83	74938.0
84-85	76025.0
86-87	80307.0
88-89	85368.0
90-91	91735.0
92-93	94255.0
94-95	107237.0
96-97	156930.0
98-99	676906.0
100-101	5029612.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.87124908827387
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.4337650531435	75.90355350727506
2	7.337469340056016	12.454803760749332
3	1.8148218885326246	4.62078601657512
4	0.6811626499387285	2.3124449973031416
5	0.28673218269495476	1.216765924956398
6	0.15260983308124326	0.7771312294054857
7	0.08847932184764577	0.5256545394585168
8	0.05561921802599217	0.37763780057431956
9	0.03628243237810044	0.2771401820301194
>10	0.1113923700230827	1.39382414188498
>50	0.001269769198834014	0.07227025698119233
>100	3.7270865315612226E-4	0.05779361762220871
>500	2.3232426141123525E-5	0.010194025184152296
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2375454968132585E-5
2	0.0	1.2375454968132585E-5	0.0	0.0	1.2375454968132585E-5
3	0.0	1.2375454968132585E-5	0.0	0.0	1.2375454968132585E-5
4	0.0	4.950181987253034E-5	0.0	0.0	1.2375454968132585E-5
5	0.0	4.950181987253034E-5	0.0	0.0	1.2375454968132585E-5
6	9.900363974506068E-5	4.950181987253034E-5	0.0	0.0	2.475090993626517E-5
7	9.900363974506068E-5	4.950181987253034E-5	0.0	0.0	2.475090993626517E-5
8	1.1137909471319327E-4	4.950181987253034E-5	0.0	0.0	2.475090993626517E-5
9	1.1137909471319327E-4	8.662818477692809E-5	0.0	3.712636490439775E-5	4.950181987253034E-5
10-11	1.1137909471319327E-4	8.662818477692809E-5	0.0	4.950181987253034E-5	4.950181987253034E-5
12-13	1.1137909471319327E-4	9.281591226099439E-5	0.0	4.950181987253034E-5	4.950181987253034E-5
14-15	1.1137909471319327E-4	1.2994227716539214E-4	0.0	6.187727484066293E-5	7.42527298087955E-5
16-17	1.7325636955385618E-4	1.3613000464945843E-4	0.0	6.187727484066293E-5	8.662818477692809E-5
18-19	1.7325636955385618E-4	1.3613000464945843E-4	0.0	8.044045729286179E-5	8.662818477692809E-5
20-21	1.7325636955385618E-4	1.3613000464945843E-4	0.0	8.662818477692809E-5	8.662818477692809E-5
22-23	1.7325636955385618E-4	1.3613000464945843E-4	0.0	2.8463546426704943E-4	8.662818477692809E-5
24-25	1.7325636955385618E-4	1.5469318710165732E-4	0.0	0.0010395382173231371	8.662818477692809E-5
26-27	1.7325636955385618E-4	1.7325636955385618E-4	0.0	0.0032176182917144722	8.662818477692809E-5
28-29	1.7325636955385618E-4	1.7944409703792247E-4	0.0	0.017548395144812004	8.662818477692809E-5
30-31	1.7325636955385618E-4	1.8563182452198878E-4	0.0	0.0248375381210421	8.662818477692809E-5
32-33	1.7325636955385618E-4	1.8563182452198878E-4	0.0	0.027591076851451596	8.662818477692809E-5
34-35	1.7325636955385618E-4	2.413213718785854E-4	0.0	0.03103764106007652	8.662818477692809E-5
36-37	1.8563182452198878E-4	3.0938637420331464E-4	0.0	0.03552993121350865	8.662818477692809E-5
38-39	1.8563182452198878E-4	3.465127391077124E-4	0.0	0.043524475122922304	8.662818477692809E-5
40-41	1.8563182452198878E-4	3.960145589802427E-4	0.0	0.05911754838276936	8.662818477692809E-5
42-43	1.8563182452198878E-4	4.2076546891650786E-4	0.0	0.06685220773785222	8.662818477692809E-5
44-45	1.9800727949012136E-4	4.3932865136870675E-4	0.0	0.07725377763856767	9.281591226099439E-5
46-47	2.1038273445825393E-4	4.5170410633683933E-4	0.0	0.09042126172466072	1.0519136722912697E-4
48-49	2.1657046194232025E-4	4.6407956130497196E-4	0.0	0.10169530120062951	1.2375454968132586E-4
50-51	2.2275818942638653E-4	5.197691086615686E-4	0.0	0.11311165840873183	1.2375454968132586E-4
52-53	2.2275818942638653E-4	5.197691086615686E-4	0.0	0.1278570130032618	1.48505459617591E-4
54-55	2.2275818942638653E-4	5.507077460819E-4	0.0	0.15509538938812162	1.7944409703792247E-4
56-57	2.351336443945191E-4	5.692709285340989E-4	0.0	0.18335474080785238	1.8563182452198878E-4
58-59	2.351336443945191E-4	5.692709285340989E-4	0.0	0.21261650408000188	1.8563182452198878E-4
60-61	2.5369682684671797E-4	5.692709285340989E-4	0.0	0.2310992460749079	1.8563182452198878E-4
62-63	3.217618291714472E-4	5.878341109862978E-4	0.0	0.24124093142129255	1.9800727949012136E-4
64-65	3.217618291714472E-4	6.063972934384966E-4	0.0	0.25139499222264533	1.9800727949012136E-4
66-67	3.4651273910771236E-4	6.682745682791596E-4	0.0	0.25820149245511825	1.9800727949012136E-4
68-69	3.4651273910771236E-4	6.99213205699491E-4	0.0	0.2641850249322103	1.9800727949012136E-4
70-71	3.5888819407584494E-4	7.177763881516899E-4	0.0	0.26982204467019477	1.9800727949012136E-4
72-73	4.083900139483753E-4	7.301518431198225E-4	0.0	0.27428339618620656	2.2275818942638653E-4
74-75	4.083900139483753E-4	7.301518431198225E-4	0.0	0.2779094044918694	2.2275818942638653E-4
76-77	4.1457774143244155E-4	7.61090480540154E-4	0.0	0.2813374055180421	2.2275818942638653E-4
78-79	4.2076546891650786E-4	7.672782080242203E-4	0.0	0.28435701653026646	2.2275818942638653E-4
80-81	4.2076546891650786E-4	7.672782080242203E-4	0.0	0.2855079338423028	2.2894591691045282E-4
82-83	4.269531964005742E-4	7.734659355082866E-4	0.0	0.28600913976851217	2.598845543307843E-4
84-85	4.3314092388464044E-4	7.796536629923528E-4	0.0	0.28632471387019953	2.598845543307843E-4
86-87	4.45516378852773E-4	7.920291179604854E-4	0.0	0.2863618402351039	2.598845543307843E-4
88-89	4.826427437571708E-4	8.167800278967506E-4	0.0	0.28636802796258803	2.7226000929891686E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10655	0.0	48.723896	1
GTATCAA	19795	0.0	38.67221	1
ATCAACG	24725	0.0	30.547306	3
TCAACGC	24780	0.0	30.462305	4
CAACGCA	25280	0.0	29.893528	5
TATCAAC	25715	0.0	29.487474	2
AACGCAG	25880	0.0	29.266356	6
ACGCAGA	29615	0.0	25.604113	7
CGCAGAG	30335	0.0	24.968298	8
GCAGAGT	34860	0.0	21.60542	9
GTACATG	24610	0.0	21.07789	1
TACATGG	24295	0.0	20.54415	2
ACATGGG	24805	0.0	19.692047	3
AGAGTAC	31970	0.0	19.526505	10-11
GAGTACT	21135	0.0	19.392097	12-13
CAGAGTA	34325	0.0	18.317205	10-11
AGTACTT	23020	0.0	17.841204	12-13
GTGGTAT	5245	0.0	17.390676	1
GTACTTT	23600	0.0	17.340214	14-15
CATGGGG	17820	0.0	17.173635	4
>>END_MODULE