FastQCFastQC Report
Fri 27 May 2016
SRR2139026_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139026_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16863221
Sequences flagged as poor quality0
Sequence length19-101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA231400.044.1964381
GTATCAA413150.034.3815081
ATCAACG499200.027.8106653
TCAACGC500650.027.721564
CAACGCA507300.027.332825
TATCAAC527200.026.6428852
AACGCAG523450.026.5714056
ACGCAGA597000.023.269117
CGCAGAG598100.023.2119798
GCAGAGT695600.019.8107229
AGAGTAC633250.019.05449910-11
GTACATG454450.018.8352811
GAGTACT424000.018.571112-13
TACATGG448850.018.4209162
ACATGGG454150.017.7810173
AGTACTT468450.017.37167512-13
CAGAGTA687550.017.2410510-11
GTACTTT489300.016.07139614-15
CATGGGG319500.016.0045914
GTGGTAT130150.015.2481451
Produced by FastQC (version 0.11.5)