FastQCFastQC Report
Fri 27 May 2016
SRR2139025_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139025_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13669357
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA182150.050.532431
GTATCAA329550.037.1758651
ATCAACG419200.028.7875163
TCAACGC419600.028.7600734
CAACGCA430000.028.0446325
TATCAAC434700.027.9379482
AACGCAG442050.027.3766846
ACGCAGA507550.023.810067
CGCAGAG523850.023.0773378
GTACATG410350.020.0494211
GCAGAGT598950.020.0344379
TACATGG411750.019.6081562
GAGTACT362700.019.27270112-13
ACATGGG414900.018.9346123
AGAGTAC550800.018.80408110-11
GTGGTAT91350.018.7320381
CAGAGTA587950.017.85908510-11
TGGTATC92700.017.354452
AGTACTT396000.017.20481512-13
GTACTTT410150.017.02822514-15
Produced by FastQC (version 0.11.5)