##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139025_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13669357
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.61422874536088	34.0	31.0	34.0	31.0	34.0
2	32.73853605549991	34.0	31.0	34.0	31.0	34.0
3	32.805681642523496	34.0	31.0	34.0	31.0	34.0
4	36.28090809245819	37.0	37.0	37.0	35.0	37.0
5	36.21947894110893	37.0	37.0	37.0	35.0	37.0
6	36.257466170500926	37.0	37.0	37.0	35.0	37.0
7	36.2056131828293	37.0	37.0	37.0	35.0	37.0
8	36.18462689942182	37.0	37.0	37.0	35.0	37.0
9	37.90703564183743	39.0	38.0	39.0	35.0	39.0
10-11	37.86373938437631	39.0	38.0	39.0	35.0	39.0
12-13	37.83558297584882	39.0	38.0	39.0	35.0	39.0
14-15	39.22316148447948	41.0	39.0	41.0	36.0	41.0
16-17	39.194493932669985	40.5	39.0	41.0	36.0	41.0
18-19	39.20643692311204	40.0	39.0	41.0	36.0	41.0
20-21	39.20088956018611	40.0	39.0	41.0	36.0	41.0
22-23	39.07000851285435	40.0	39.0	41.0	35.5	41.0
24-25	39.017029976038685	40.0	38.5	41.0	35.0	41.0
26-27	38.96367197757296	40.0	38.5	41.0	35.0	41.0
28-29	38.77472224632295	40.0	38.0	41.0	35.0	41.0
30-31	38.66371648255354	40.0	38.0	41.0	35.0	41.0
32-33	38.55001014042863	40.0	38.0	41.0	34.5	41.0
34-35	38.26791988661641	40.0	38.0	41.0	34.0	41.0
36-37	38.516795903537314	40.0	38.0	41.0	34.0	41.0
38-39	38.618240484004104	40.0	38.0	41.0	35.0	41.0
40-41	38.61073586313765	40.0	38.0	41.0	35.0	41.0
42-43	38.55277789223872	40.0	38.0	41.0	34.0	41.0
44-45	38.50410393896064	40.0	38.0	41.0	34.0	41.0
46-47	38.43315325168309	40.0	38.0	41.0	34.0	41.0
48-49	38.373136768505574	40.0	38.0	41.0	34.0	41.0
50-51	38.29953266282888	40.0	38.0	41.0	34.0	41.0
52-53	38.1897783560488	40.0	37.5	41.0	33.5	41.0
54-55	38.047440566367314	40.0	37.0	41.0	33.0	41.0
56-57	37.9072437247641	40.0	37.0	41.0	33.0	41.0
58-59	37.74294480331839	39.5	36.0	41.0	33.0	41.0
60-61	37.544268722835	39.0	36.0	41.0	33.0	41.0
62-63	37.30859893148006	39.0	35.5	40.0	32.5	41.0
64-65	37.02396080404049	38.5	35.0	40.0	32.0	41.0
66-67	36.737355585037	38.0	35.0	40.0	32.0	41.0
68-69	36.44930569582884	37.5	35.0	40.0	31.0	41.0
70-71	36.13797251507088	37.0	35.0	39.5	31.0	41.0
72-73	35.8342821966494	36.5	34.5	39.0	31.0	40.5
74-75	35.5175521035882	36.0	34.0	39.0	31.0	40.0
76-77	34.473994048965295	35.0	33.5	37.0	29.5	39.0
78-79	34.76044043396389	35.0	34.0	37.0	30.0	39.0
80-81	34.56284606676978	35.0	34.0	37.0	30.0	39.0
82-83	34.32325211661242	35.0	34.0	36.0	30.0	38.5
84-85	34.071825417792056	35.0	34.0	36.0	30.0	37.0
86-87	33.829715731480206	35.0	34.0	35.5	30.0	37.0
88-89	33.63662719655302	35.0	33.0	35.0	30.0	36.5
90-91	33.47203337368654	35.0	33.0	35.0	30.0	36.0
92-93	33.347403862256996	35.0	33.0	35.0	30.0	36.0
94-95	33.27771501855282	34.5	33.0	35.0	29.5	35.5
96-97	33.23583269190917	34.0	33.0	35.0	29.5	35.0
98-99	33.252938122992944	34.0	33.0	35.0	30.0	35.0
100-101	32.211749433570056	33.0	31.0	34.5	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	0.0
14	1.0
15	0.0
16	0.0
17	1.0
18	2.0
19	19.0
20	86.0
21	446.0
22	1955.0
23	6426.0
24	15599.0
25	32010.0
26	55167.0
27	85978.0
28	122855.0
29	164496.0
30	210322.0
31	264052.0
32	334328.0
33	434990.0
34	611017.0
35	887852.0
36	1534412.0
37	3097399.0
38	4015825.0
39	1723801.0
40	70317.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.64446703187291	21.665391063835205	15.443882717500767	24.246259186791125
2	13.56612308171771	22.20193238340699	41.44500052307645	22.786944011798848
3	16.63587271730218	27.28502419134426	31.59839617854599	24.48070691280757
4	11.818745188598173	19.24448070073251	39.5375479731923	29.399226137477026
5	12.667197001409136	38.277163646504434	36.052515722396244	13.00312362969018
6	28.70184438109918	39.12973216366098	19.1512474436431	13.017176011596742
7	25.41565421338461	33.29042957769405	23.979112401426402	17.31480380749494
8	23.95334993641614	37.014169438500694	21.57341948032785	17.45906114475532
9	25.262492249086204	16.88113909682358	21.759042635219977	36.09732601887024
10-11	23.579243705464712	27.211214104657593	29.471214337294725	19.73832785258297
12-13	24.563141485001818	24.91034508792184	29.488921827120322	21.037591599956016
14-15	21.73246442102428	26.14521539860915	27.290075072387715	24.832245107978853
16-17	21.107852272366053	29.304265179415918	27.89127747246969	21.69660507574834
18-19	21.154101005318505	28.433353759811318	29.72426742033335	20.68827781453682
20-21	21.99576515058238	27.50255450980444	29.667085937682323	20.834594401930858
22-23	21.817681967205917	27.492535325961704	29.375861062314023	21.31392164451836
24-25	21.499053328196403	27.658993678523697	29.49130665737345	21.350646335906447
26-27	21.460874036938286	27.792580626881662	29.448163826027823	21.298381510152222
28-29	21.404489463120093	27.885270305030986	29.447546658368285	21.26269357348064
30-31	21.6606607230787	27.738589795493805	29.3486596944869	21.2520897869406
32-33	21.5144007305907	27.784375170165347	29.291338303995673	21.409885795248275
34-35	21.62012885236181	27.890865025507956	29.25341469097107	21.235591431159172
36-37	21.62695023266804	27.872270899749225	29.169307382088956	21.33147148549378
38-39	21.507852211784908	27.950030340498238	29.200124392505938	21.34199305521092
40-41	21.66813600778893	27.823761186586243	29.066740605770157	21.44136219985467
42-43	21.618334752291037	27.97009204679206	28.793679927289595	21.617893273627306
44-45	21.67507897865388	28.068212802618298	28.63465085961933	21.62205735910849
46-47	21.774672805484542	28.03427111545543	28.34286246326506	21.84819361579497
48-49	21.78198872748298	28.221917767067122	28.190432934687177	21.805660570762726
50-51	21.811615722920443	28.260855184498922	28.12391415470786	21.80361493787277
52-53	21.91642004759148	28.223677995201406	28.02459846535713	21.835303491849984
54-55	21.901029943593294	28.173030396462533	27.990503262073744	21.935436397870433
56-57	21.93020635233135	28.258119750426168	27.9481268272623	21.86354706998018
58-59	21.820720412002302	28.32424419888815	28.018871441931214	21.83616394717834
60-61	21.961189703372042	28.31752579657752	27.939072517998632	21.78221198205181
62-63	21.934618105707866	28.368838855953655	27.910630302658753	21.785912735679723
64-65	21.91514948351892	28.24831769764889	27.905987982742264	21.930544836089926
66-67	21.949940948726397	28.236642635438642	27.81384138383663	21.99957503199833
68-69	21.91868757406019	28.214604891666266	27.895215230182984	21.971492304090557
70-71	21.8829149490347	28.095689135784646	27.92034783608308	22.10104807909757
72-73	21.97016050788397	28.124021747079425	27.861101054857667	22.044716690178934
74-75	21.892133617958297	28.17123166144596	27.854368181917533	22.082266538678212
76-77	21.94210911568022	28.12429261399096	27.802390109677006	22.131208160651813
78-79	21.924084542196148	28.090110121456696	27.81704158798128	22.168763748365876
80-81	21.960482779123094	28.107567483871993	27.828230513412667	22.103719223592247
82-83	22.023119646258632	28.02229461183625	27.925191532520348	22.02939420938477
84-85	22.07261204428506	28.028243010236586	27.86052841734326	22.03861652813509
86-87	21.948440625632326	28.04442739943379	27.86862818211323	22.13850379282065
88-89	22.019415760150586	27.940424128572833	27.87101328212998	22.1691468291466
90-91	22.072269026898724	27.980136495832713	27.869863547287576	22.07773092998099
92-93	22.12736109705889	27.86150954556421	27.94791285635346	22.063216501023433
94-95	21.879093104871068	27.93293916290649	28.018189424420363	22.16977830780208
96-97	21.896039752839883	27.989818883545986	28.00035221943835	22.113789144175776
98-99	22.10979517558381	28.536619882356433	28.065241894865423	21.28834304719433
100-101	22.642550291091474	31.246412646446704	25.270964698296922	20.8400723641649
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3244.0
1	2954.5
2	4173.5
3	5965.0
4	7956.5
5	11922.0
6	15112.0
7	18076.0
8	20397.0
9	20391.0
10	18605.5
11	17098.5
12	19287.0
13	26653.5
14	43848.0
15	66950.0
16	84524.5
17	93291.0
18	93631.5
19	87772.0
20	79649.0
21	72135.0
22	69162.0
23	73976.0
24	85547.5
25	104972.5
26	130452.0
27	158615.0
28	192334.5
29	228516.0
30	262529.5
31	296233.0
32	329163.0
33	360875.5
34	391028.0
35	417746.0
36	444862.5
37	470195.0
38	491767.5
39	509716.0
40	524051.0
41	535343.5
42	541374.0
43	546604.0
44	552107.0
45	558465.5
46	562052.5
47	562548.0
48	557084.5
49	542400.0
50	525794.5
51	506929.0
52	478816.5
53	444603.0
54	412624.5
55	381201.0
56	348023.0
57	311294.0
58	272175.0
59	231735.5
60	192183.5
61	157157.5
62	124790.5
63	96282.5
64	73250.5
65	54598.5
66	41355.0
67	31077.5
68	22250.0
69	15952.0
70	11565.0
71	8489.0
72	5867.5
73	3889.5
74	2709.5
75	1884.5
76	1243.5
77	739.5
78	489.5
79	358.5
80	224.0
81	135.5
82	95.5
83	62.0
84	45.5
85	36.0
86	28.0
87	25.0
88	17.5
89	10.0
90	6.5
91	5.0
92	4.0
93	2.5
94	1.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.194689918479706E-5
2	0.0016606487049829774
3	3.6578165307995104E-5
4	2.9262532246396085E-5
5	2.194689918479706E-5
6	1.4631266123198042E-5
7	1.4631266123198042E-5
8	5.852506449279217E-5
9	7.315633061599021E-6
10-11	0.0
12-13	0.0
14-15	6.108553606435182E-4
16-17	0.0027616514807536304
18-19	5.486724796199265E-5
20-21	0.0
22-23	0.001064541544209331
24-25	0.005224964543968717
26-27	0.009864474564461486
28-29	0.36039798849269683
30-31	0.3289758938623739
32-33	0.3319383881256185
34-35	0.1877083775265303
36-37	0.0468204537735354
38-39	0.03702258202587882
40-41	0.022454671247694277
42-43	0.029838070563981405
44-45	0.019034072206943975
46-47	0.014448355335909463
48-49	0.00903089108077839
50-51	0.004826072345055483
52-53	0.003715666032939182
54-55	5.843696386818672E-4
56-57	0.0032350433363519055
58-59	0.005665139316531875
60-61	0.004503202352009536
62-63	0.003620607262238732
64-65	6.184667959082403E-4
66-67	6.705604997285069E-5
68-69	7.535613818927159E-4
70-71	0.0010776958861953144
72-73	0.0017666824933773163
74-75	0.002080939743663124
76-77	9.519870614380716E-4
78-79	8.90924274688525E-5
80-81	0.0031488951702615276
82-83	0.0014809025540602338
84-85	6.4525070363437E-5
86-87	1.8657877313821672E-5
88-89	0.0015449592350457984
90-91	0.003880675271992218
92-93	0.014605148142065146
94-95	0.0015519577155775532
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	233.0
20-21	854.0
22-23	1965.0
24-25	4706.0
26-27	7287.0
28-29	11818.0
30-31	27056.0
32-33	36174.0
34-35	51482.0
36-37	71783.0
38-39	84825.0
40-41	90918.0
42-43	109245.0
44-45	129390.0
46-47	128967.0
48-49	112977.0
50-51	99432.0
52-53	97263.0
54-55	103486.0
56-57	107553.0
58-59	107397.0
60-61	105899.0
62-63	105352.0
64-65	112826.0
66-67	120139.0
68-69	119144.0
70-71	117437.0
72-73	113927.0
74-75	116054.0
76-77	118418.0
78-79	124615.0
80-81	126608.0
82-83	124374.0
84-85	127452.0
86-87	134370.0
88-89	143526.0
90-91	155174.0
92-93	159146.0
94-95	180294.0
96-97	269183.0
98-99	1152641.0
100-101	8557967.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.90431322949567
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.44183176857287	73.32209323532128
2	7.73469427174497	12.824790332782621
3	2.0732635531322248	5.156474730485133
4	0.8032407219198062	2.663684816349034
5	0.37488925988524385	1.5539968313950034
6	0.18794122767744142	0.9348683044863939
7	0.11513889442420686	0.6681857677769596
8	0.07333757529743358	0.4864001051160126
9	0.04709950053077823	0.3514276570460793
>10	0.1453613835021995	1.8127505255499
>50	0.0027280480011196862	0.15434077114994793
>100	4.430472549864544E-4	0.05700533252842205
>500	3.074805671916055E-5	0.013981590013182311
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.31563306159902E-6
2	0.0	5.120943143119314E-5	0.0	0.0	7.31563306159902E-6
3	0.0	5.852506449279216E-5	0.0	0.0	7.31563306159902E-6
4	0.0	1.2436576204718334E-4	0.0	0.0	7.31563306159902E-6
5	0.0	1.2436576204718334E-4	0.0	0.0	7.31563306159902E-6
6	0.0	1.3168139510878236E-4	0.0	0.0	7.31563306159902E-6
7	7.31563306159902E-6	1.3168139510878236E-4	0.0	0.0	1.463126612319804E-5
8	7.31563306159902E-6	1.4631266123198042E-4	0.0	0.0	1.463126612319804E-5
9	7.31563306159902E-6	2.1946899184797062E-4	0.0	0.0	1.463126612319804E-5
10-11	7.31563306159902E-6	2.1946899184797062E-4	0.0	0.0	1.463126612319804E-5
12-13	7.31563306159902E-6	2.3044244144036915E-4	0.0	0.0	1.463126612319804E-5
14-15	7.31563306159902E-6	3.292034877719559E-4	0.0	0.0	1.8289082653997552E-5
16-17	7.31563306159902E-6	3.548082034875525E-4	0.0	0.0	2.1946899184797062E-5
18-19	7.31563306159902E-6	4.0601763491874567E-4	0.0	0.0	3.6578165307995104E-5
20-21	7.31563306159902E-6	4.5722706634993877E-4	0.0	0.0	5.852506449279216E-5
22-23	1.0973449592398531E-5	5.2306776390433E-4	0.0	0.0	5.852506449279216E-5
24-25	2.926253224639608E-5	6.071975441127187E-4	0.0	0.0	5.852506449279216E-5
26-27	3.6578165307995104E-5	6.510913424823128E-4	0.0	1.0973449592398531E-5	5.852506449279216E-5
28-29	3.6578165307995104E-5	6.584069755439118E-4	0.0	1.6825956041677747E-4	6.94985140851907E-5
30-31	4.023598183879462E-5	6.949851408519069E-4	0.0	7.059585904443054E-4	8.047196367758922E-5
32-33	4.3893798369594123E-5	6.949851408519069E-4	0.0	0.00179233010009176	8.047196367758922E-5
34-35	4.3893798369594123E-5	8.266665359606892E-4	0.0	0.0034676100711979354	8.047196367758922E-5
36-37	4.3893798369594123E-5	9.620057476002711E-4	0.0	0.006141473955212378	8.778759673918825E-5
38-39	4.3893798369594123E-5	0.0010278464451546623	0.0	0.010388198947470608	8.778759673918825E-5
40-41	4.3893798369594123E-5	0.0011266074914862492	0.0	0.014920233629131203	8.778759673918825E-5
42-43	4.3893798369594123E-5	0.0011522122072018456	0.0	0.01935350726446021	9.144541326998776E-5
44-45	5.120943143119314E-5	0.0013131561345570242	0.0	0.024258639232262354	9.510322980078726E-5
46-47	7.681414714678971E-5	0.0014375218966042075	0.0	0.029774626560708013	9.510322980078726E-5
48-49	8.047196367758922E-5	0.0014521531627274056	0.0	0.03541863746773166	9.510322980078726E-5
50-51	8.047196367758922E-5	0.0016130970900825842	0.0	0.04097120296148531	9.510322980078726E-5
52-53	8.047196367758922E-5	0.0016313861727365816	0.0	0.04635916671135299	9.510322980078726E-5
54-55	8.047196367758922E-5	0.001649675255390579	0.0	0.05184223369102146	9.876104633158677E-5
56-57	9.510322980078726E-5	0.0016825956041677747	0.0	0.05792884039827184	1.060766793931858E-4
58-59	1.0973449592398531E-4	0.0016935690537601732	0.0	0.06362771855325748	1.0973449592398531E-4
60-61	1.0973449592398531E-4	0.0017484363017221658	0.0	0.0693339123413047	1.1339231245478481E-4
62-63	1.1705012898558432E-4	0.0017667253843761635	0.0	0.07462311504484082	1.2436576204718334E-4
64-65	1.1705012898558432E-4	0.002077639789494122	0.0	0.08021957433696406	1.2436576204718334E-4
66-67	1.2070794551638384E-4	0.002106902321740518	0.0	0.08602818698787368	1.2802357857798284E-4
68-69	1.2436576204718334E-4	0.002143480487048513	0.0	0.0917819323908213	1.389970281703814E-4
70-71	1.2436576204718334E-4	0.0021983477350105054	0.0	0.09732352443498257	1.4631266123198042E-4
72-73	1.2436576204718334E-4	0.0022276102672569016	0.0	0.1016287744917336	1.4997047776277992E-4
74-75	1.3168139510878236E-4	0.0022971087813420926	0.0	0.10519880342579391	1.6094392735517844E-4
76-77	1.3168139510878236E-4	0.0023995276442044786	0.0	0.10814700354961831	1.6825956041677747E-4
78-79	1.3168139510878236E-4	0.0024470792591048725	0.0	0.11106594114119633	1.71917376947577E-4
80-81	1.3168139510878236E-4	0.002450737075635672	0.0	0.11228765186248336	1.9020645960157452E-4
82-83	1.3168139510878236E-4	0.002469026158289669	0.0	0.1127777992776105	2.0483772572477257E-4
84-85	1.3168139510878236E-4	0.0025019465070668647	0.0	0.11304847770088966	2.0483772572477257E-4
86-87	1.389970281703814E-4	0.002531209039313261	0.0	0.11315821219681364	2.158111753171711E-4
88-89	1.389970281703814E-4	0.00268849515013764	0.0	0.11317284346293685	2.4141589103276768E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	18215	0.0	50.53243	1
GTATCAA	32955	0.0	37.175865	1
ATCAACG	41920	0.0	28.787516	3
TCAACGC	41960	0.0	28.760073	4
CAACGCA	43000	0.0	28.044632	5
TATCAAC	43470	0.0	27.937948	2
AACGCAG	44205	0.0	27.376684	6
ACGCAGA	50755	0.0	23.81006	7
CGCAGAG	52385	0.0	23.077337	8
GTACATG	41035	0.0	20.049421	1
GCAGAGT	59895	0.0	20.034437	9
TACATGG	41175	0.0	19.608156	2
GAGTACT	36270	0.0	19.272701	12-13
ACATGGG	41490	0.0	18.934612	3
AGAGTAC	55080	0.0	18.804081	10-11
GTGGTAT	9135	0.0	18.732038	1
CAGAGTA	58795	0.0	17.859085	10-11
TGGTATC	9270	0.0	17.35445	2
AGTACTT	39600	0.0	17.204815	12-13
GTACTTT	41015	0.0	17.028225	14-15
>>END_MODULE