FastQCFastQC Report
Fri 27 May 2016
SRR2139024_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139024_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12237835
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA167100.052.4880371
GTATCAA310100.036.8293571
ATCAACG387850.028.5863363
TCAACGC388800.028.5164874
CAACGCA396350.027.9732825
TATCAAC407600.027.6211012
AACGCAG405800.027.3745236
ACGCAGA465400.023.8597077
CGCAGAG473000.023.4763368
GTACATG364150.021.4563221
TACATGG364750.020.9874522
GCAGAGT539000.020.4513519
ACATGGG366700.020.4325853
GAGTACT335450.019.7577312-13
AGAGTAC497200.019.2235610-11
CATGGGG277400.018.5358074
CAGAGTA534750.018.357310-11
GTACTTT377950.017.42912514-15
GTGGTAT80450.017.426381
AGTACTT368400.017.36981812-13
Produced by FastQC (version 0.11.5)