##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139024_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12237835
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.66275325660135	34.0	31.0	34.0	31.0	34.0
2	32.7727071822753	34.0	31.0	34.0	31.0	34.0
3	32.84004090592821	34.0	31.0	34.0	31.0	34.0
4	36.30191712831559	37.0	37.0	37.0	35.0	37.0
5	36.24523046764399	37.0	37.0	37.0	35.0	37.0
6	36.282147209861876	37.0	37.0	37.0	35.0	37.0
7	36.23175455462506	37.0	37.0	37.0	35.0	37.0
8	36.21004867282489	37.0	37.0	37.0	35.0	37.0
9	37.9391881815697	39.0	38.0	39.0	35.0	39.0
10-11	37.89695366868405	39.0	38.0	39.0	35.0	39.0
12-13	37.87086633379188	39.0	38.0	39.0	35.0	39.0
14-15	39.2690675270585	41.0	39.0	41.0	36.0	41.0
16-17	39.24392611111361	41.0	39.0	41.0	36.0	41.0
18-19	39.259394370000905	41.0	39.0	41.0	36.0	41.0
20-21	39.254092270058855	41.0	39.0	41.0	36.0	41.0
22-23	39.12497298681805	40.0	39.0	41.0	35.5	41.0
24-25	39.07338189116672	40.0	39.0	41.0	35.0	41.0
26-27	39.02092113168713	40.0	39.0	41.0	35.0	41.0
28-29	38.82831018925462	40.0	38.5	41.0	35.0	41.0
30-31	38.71627817560441	40.0	38.0	41.0	35.0	41.0
32-33	38.60233231172967	40.0	38.0	41.0	35.0	41.0
34-35	38.32599667809427	40.0	38.0	41.0	34.0	41.0
36-37	38.573484502891226	40.0	38.0	41.0	34.5	41.0
38-39	38.676539298910896	40.0	38.0	41.0	35.0	41.0
40-41	38.66740709686613	40.0	38.0	41.0	35.0	41.0
42-43	38.611348932456764	40.0	38.0	41.0	34.5	41.0
44-45	38.56238891359063	40.0	38.0	41.0	34.0	41.0
46-47	38.49538535139781	40.0	38.0	41.0	34.0	41.0
48-49	38.43397299349542	40.0	38.0	41.0	34.0	41.0
50-51	38.362841385504375	40.0	38.0	41.0	34.0	41.0
52-53	38.25656268454876	40.0	37.5	41.0	34.0	41.0
54-55	38.11893997343406	40.0	37.0	41.0	33.0	41.0
56-57	37.98228090108595	40.0	37.0	41.0	33.0	41.0
58-59	37.8190586195044	40.0	36.5	41.0	33.0	41.0
60-61	37.62686266504073	39.0	36.0	41.0	33.0	41.0
62-63	37.39673943874281	39.0	35.5	40.5	33.0	41.0
64-65	37.11675535548481	38.5	35.0	40.0	32.0	41.0
66-67	36.83540785128737	38.0	35.0	40.0	32.0	41.0
68-69	36.54926487472481	37.5	35.0	40.0	32.0	41.0
70-71	36.243938977139074	37.0	35.0	39.5	31.0	41.0
72-73	35.9415087110595	36.5	35.0	39.0	31.0	40.5
74-75	35.62733546664051	36.0	34.0	39.0	31.0	40.0
76-77	34.58245940912741	35.0	33.5	37.0	29.5	39.0
78-79	34.85981741464135	35.0	34.0	37.0	30.5	39.0
80-81	34.65885353492055	35.0	34.0	37.0	31.0	39.0
82-83	34.41758656790745	35.0	34.0	36.5	30.0	38.5
84-85	34.163855564679054	35.0	34.0	36.0	30.0	37.0
86-87	33.91536888360992	35.0	34.0	35.5	30.0	37.0
88-89	33.720530038965975	35.0	33.5	35.0	30.0	36.5
90-91	33.55239767823251	35.0	33.0	35.0	30.0	36.0
92-93	33.426198110028636	35.0	33.0	35.0	30.0	36.0
94-95	33.3516252399724	35.0	33.0	35.0	30.0	36.0
96-97	33.30508664825766	34.0	33.0	35.0	30.0	35.0
98-99	33.31397549917607	34.0	33.0	35.0	30.0	35.0
100-101	32.29087081622999	33.0	31.0	34.5	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	4.0
19	8.0
20	69.0
21	392.0
22	1643.0
23	5389.0
24	13401.0
25	26775.0
26	47110.0
27	73406.0
28	105517.0
29	140936.0
30	182049.0
31	229828.0
32	291735.0
33	379552.0
34	536558.0
35	774063.0
36	1323424.0
37	2714324.0
38	3667664.0
39	1653314.0
40	70673.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.057372330542506	21.547679233733618	15.326749433498563	24.068199002225313
2	13.591145403902818	22.102578425775857	41.65291263286282	22.653363537458503
3	16.578147454700517	27.431604925479864	31.735479639246588	24.254767980573032
4	11.76749184271431	19.147654654218602	39.79915398420619	29.2856995188609
5	12.54194267890402	38.47145977559916	36.11271701452373	12.87388053097309
6	28.596696817157092	39.27924984758331	19.202599022228853	12.921454313030747
7	25.239313201936973	33.54540791658131	24.087867517067767	17.127411364413945
8	23.92246171194636	37.089057900403056	21.613293211824814	17.375187175825772
9	25.410090918859424	16.80654298738298	21.812689907978005	35.970676185779595
10-11	23.520185555696738	27.272340246456995	29.605060862480986	19.60241333536528
12-13	24.513563877924486	25.02849155916876	29.639029289085855	20.818915273820902
14-15	21.69353720409294	26.293425762065958	27.48276174555901	24.530275288282088
16-17	20.985700700409296	29.38414033109329	28.097291212759156	21.53286775573826
18-19	21.022626894678844	28.49498759011609	30.002538849629012	20.47984666557606
20-21	21.91069298041431	27.61742683737663	29.87166517881968	20.600215003389383
22-23	21.6963525246164	27.502334437202453	29.66334988347019	21.137963154710953
24-25	21.364791786756562	27.731590640694836	29.73840032270027	21.165217249848332
26-27	21.32650131232673	27.85689394553384	29.658817132077242	21.15778761006219
28-29	21.27072279206547	27.984967373079574	29.632418255307297	21.111891579547663
30-31	21.52144450582132	27.777006285826	29.568447585629066	21.13310162272361
32-33	21.375335618311805	27.90155259340608	29.474545974925864	21.248565813356247
34-35	21.481267442117076	27.998070345755842	29.444127166607164	21.07653504551992
36-37	21.448880363212773	27.992253598403398	29.40287265081057	21.155993387573254
38-39	21.35693439124008	28.053573612871997	29.438989085080525	21.1505029108074
40-41	21.555448138412025	27.97124280579401	29.248599037087946	21.224710018706013
42-43	21.478695854343226	28.08772153921193	29.015778022568217	21.417804583876627
44-45	21.63762202059834	28.17264612321208	28.75542859514566	21.43430326104392
46-47	21.646576916674274	28.20266672394628	28.502903539343038	21.64785282003641
48-49	21.635234959598648	28.372860447627225	28.369312607760754	21.62259198501337
50-51	21.626382404662223	28.44685627400267	28.252595544656522	21.674165776678585
52-53	21.762518323305642	28.36698715822702	28.118809638203107	21.75168488026423
54-55	21.793300274391644	28.270449657097057	28.166290272763362	21.769959795747937
56-57	21.794244791708653	28.37723161622247	28.08244607907155	21.746077512997328
58-59	21.7449447675213	28.455245442425582	28.14233178604249	21.657478004010624
60-61	21.850187017828823	28.353267733551107	28.1520338801055	21.644511368514575
62-63	21.837726390414478	28.412057028325478	28.048243696769788	21.701972884490257
64-65	21.791997959695838	28.38734418307578	28.054020055329904	21.766637801898476
66-67	21.866730686105228	28.395567542518812	27.912578107877618	21.825123663498342
68-69	21.830020487573844	28.308794184094584	28.050099481250363	21.81108584708122
70-71	21.76827778812086	28.182301996084448	28.06790162588956	21.98151858990513
72-73	21.87264826407867	28.194215583496423	28.038660792282638	21.89447536014227
74-75	21.728053746126417	28.285042250643365	28.032778910191237	21.954125093038982
76-77	21.818219167526358	28.242345743275543	27.962726926754396	21.9767081624437
78-79	21.820784357743896	28.234579208092903	27.946432385128173	21.998204049035024
80-81	21.8193412276614	28.201859363684918	27.999280957911125	21.979518450742553
82-83	21.818529398496334	28.18531125775599	28.09136250633505	21.904796837412626
84-85	21.983553395202836	28.14462291983241	27.970727337131663	21.901096347833093
86-87	21.797376595922692	28.188326378602184	28.02267951788735	21.991617507587772
88-89	21.882743392892824	28.095189994587095	28.034067057854866	21.987999554665212
90-91	21.913098156881937	28.103535036928363	28.030044620745027	21.95332218544468
92-93	21.95936804273296	28.000888535421296	28.082359297879222	21.95738412396652
94-95	21.784226892087784	28.07051988861304	28.14620169261347	21.99905152668571
96-97	21.760766208584414	28.099978642605723	28.171609524881593	21.967645623928274
98-99	22.033162464723468	28.69546356700709	28.15279879703584	21.118575171233605
100-101	22.54882640334942	31.416964920420416	25.38567993984869	20.64852873638148
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2791.0
1	2619.5
2	3874.0
3	5650.5
4	7654.0
5	11597.5
6	14798.5
7	17651.0
8	19914.0
9	20065.0
10	18435.0
11	16673.0
12	18192.0
13	24584.0
14	40136.5
15	61366.0
16	77875.0
17	86164.0
18	86795.5
19	81825.0
20	74312.0
21	67059.0
22	63975.5
23	68624.5
24	80620.5
25	100074.5
26	125377.0
27	153803.5
28	184164.5
29	216325.5
30	246402.5
31	276062.5
32	306220.0
33	333833.0
34	361008.0
35	384467.0
36	408697.0
37	431535.5
38	446414.0
39	456371.5
40	468733.0
41	481695.0
42	487320.5
43	489325.5
44	490527.5
45	491354.0
46	487626.5
47	481292.5
48	474230.5
49	462509.5
50	448905.5
51	432161.5
52	411824.0
53	388640.0
54	363198.0
55	335305.0
56	305566.5
57	275335.5
58	243120.5
59	209035.5
60	173316.0
61	140054.0
62	110637.0
63	84599.5
64	62988.5
65	47220.0
66	36316.5
67	27761.5
68	21110.0
69	15290.0
70	10706.0
71	7696.0
72	5460.0
73	3772.0
74	2601.5
75	1753.0
76	1155.5
77	724.0
78	448.0
79	298.5
80	201.0
81	135.0
82	96.0
83	67.0
84	43.0
85	28.0
86	20.5
87	16.0
88	14.5
89	10.5
90	6.0
91	4.0
92	3.0
93	2.0
94	1.5
95	1.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.63427599734757E-5
2	0.0017405039371751621
3	1.63427599734757E-5
4	1.63427599734757E-5
5	1.63427599734757E-5
6	1.63427599734757E-5
7	1.63427599734757E-5
8	8.17137998673785E-5
9	0.0
10-11	0.0
12-13	0.0
14-15	6.700531589125037E-4
16-17	0.002839554545391403
18-19	7.354241988064065E-5
20-21	0.0
22-23	0.0011277742019526512
24-25	0.00552572016338966
26-27	0.01047118320976642
28-29	0.36520503916184166
30-31	0.3341573979198117
32-33	0.33655249301867746
34-35	0.19036661279711742
36-37	0.04705583715422569
38-39	0.03698618319095219
40-41	0.023236380480770882
42-43	0.030710220308217327
44-45	0.019455891504153
46-47	0.014750188125033314
48-49	0.008925186768640467
50-51	0.0046928285663365
52-53	0.00334071419621513
54-55	5.996090815281362E-4
56-57	0.0030229595795372237
58-59	0.005564607326844393
60-61	0.004099951160470677
62-63	0.003316889920463093
64-65	3.8922802182271777E-4
66-67	9.354088059197908E-5
68-69	7.887403396429255E-4
70-71	0.0011495714578865228
72-73	0.0019509603710813335
74-75	0.0020869485013593624
76-77	9.140677084352036E-4
78-79	1.0430014083995686E-4
80-81	0.00304865354734446
82-83	0.0014173649559816738
84-85	5.6519629138746185E-5
86-87	1.559958891963279E-5
88-89	0.0014324660208000386
90-91	0.0034706904463425382
92-93	0.01531068966337674
94-95	0.0015530444821128101
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	205.0
20-21	794.0
22-23	1765.0
24-25	4943.0
26-27	6736.0
28-29	10887.0
30-31	24518.0
32-33	32846.0
34-35	46192.0
36-37	63979.0
38-39	76304.0
40-41	81873.0
42-43	98065.0
44-45	118283.0
46-47	115380.0
48-49	99933.0
50-51	88955.0
52-53	86129.0
54-55	91714.0
56-57	94796.0
58-59	94416.0
60-61	92690.0
62-63	91901.0
64-65	98264.0
66-67	103467.0
68-69	104584.0
70-71	103586.0
72-73	101118.0
74-75	103402.0
76-77	105394.0
78-79	110994.0
80-81	113524.0
82-83	111137.0
84-85	114115.0
86-87	119733.0
88-89	127386.0
90-91	137744.0
92-93	141931.0
94-95	160100.0
96-97	236863.0
98-99	1026904.0
100-101	7694285.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.14318435217686
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.97840982851739	72.26826737555272
2	7.951206716934449	13.062748783428265
3	2.187084979642392	5.389623740299259
4	0.8635332546036174	2.8373348530856086
5	0.40081137421018614	1.6461961301098331
6	0.20988884062026772	1.0344562637121189
7	0.12297392508621721	0.7071028859207549
8	0.08851893909959094	0.581698202649345
9	0.05065413482269251	0.3744802741446407
>10	0.14354305198454495	1.8153373424943633
>50	0.0026047637677971175	0.14502851063436412
>100	6.994123689376947E-4	0.09868650092117749
>500	7.077834197470312E-5	0.03903913704760114
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	8.17137998673785E-6	0.0	8.17137998673785E-6	0.0
3	0.0	8.17137998673785E-6	0.0	1.63427599734757E-5	0.0
4	0.0	4.085689993368925E-5	0.0	3.26855199469514E-5	8.17137998673785E-6
5	0.0	4.085689993368925E-5	0.0	4.085689993368925E-5	8.17137998673785E-6
6	8.988517985411635E-5	4.085689993368925E-5	0.0	4.085689993368925E-5	8.17137998673785E-6
7	9.80565598408542E-5	4.085689993368925E-5	0.0	4.085689993368925E-5	8.17137998673785E-6
8	9.80565598408542E-5	6.53710398939028E-5	0.0	8.17137998673785E-5	8.17137998673785E-6
9	1.143993198143299E-4	9.80565598408542E-5	0.0	1.7159897972149487E-4	8.17137998673785E-6
10-11	1.2257069980106775E-4	9.80565598408542E-5	0.0	2.2062725964192196E-4	8.17137998673785E-6
12-13	1.307420797878056E-4	9.80565598408542E-5	0.0	2.3697001961539766E-4	8.17137998673785E-6
14-15	1.5525621974801914E-4	1.2257069980106775E-4	0.0	2.451413996021355E-4	1.63427599734757E-5
16-17	2.0428449966844626E-4	1.307420797878056E-4	0.0	2.655698495689801E-4	2.451413996021355E-5
18-19	2.0428449966844626E-4	1.3482776978117453E-4	0.0	2.982553695159315E-4	2.451413996021355E-5
20-21	2.0428449966844626E-4	1.5525621974801914E-4	0.0	3.391122694496208E-4	2.8599829953582478E-5
22-23	2.124558796551841E-4	1.7159897972149487E-4	0.0	3.677120994032033E-4	3.26855199469514E-5
24-25	2.2062725964192196E-4	2.0019880967507732E-4	0.0	3.8814054937004786E-4	3.26855199469514E-5
26-27	2.2062725964192196E-4	2.124558796551841E-4	0.0	4.535115892639507E-4	3.26855199469514E-5
28-29	2.2062725964192196E-4	2.1654156964855305E-4	0.0	6.169391889987077E-4	3.26855199469514E-5
30-31	2.287986396286598E-4	2.5331277958887333E-4	0.0	0.0011562502681234058	3.26855199469514E-5
32-33	2.4105570960876658E-4	2.614841595756112E-4	0.0	0.0023084148462534427	3.26855199469514E-5
34-35	2.573984695822423E-4	3.023410595093005E-4	0.0	0.00399171912352144	4.90282799204271E-5
36-37	2.8191260954245584E-4	3.4319795944298974E-4	0.0	0.006614732099264289	4.90282799204271E-5
38-39	2.941696795225626E-4	3.513693394297276E-4	0.0	0.010422595173084127	4.90282799204271E-5
40-41	2.941696795225626E-4	3.595407194164654E-4	0.0	0.01485965450588278	4.90282799204271E-5
42-43	3.0234105950930045E-4	3.758834793899411E-4	0.0	0.018986201399185394	4.90282799204271E-5
44-45	3.1459812948940723E-4	4.330831392971061E-4	0.0	0.02383182973132094	5.3113969913796025E-5
46-47	3.391122694496208E-4	4.739400392307953E-4	0.0	0.02942922502223637	6.128534990053387E-5
48-49	3.4319795944298974E-4	4.739400392307953E-4	0.0	0.034716107873655755	8.17137998673785E-5
50-51	3.717977893965722E-4	5.352253891313292E-4	0.0	0.040158246944823166	8.17137998673785E-5
52-53	3.8405485937667897E-4	5.393110791246981E-4	0.0	0.04561264308597068	8.579948986074743E-5
54-55	3.8814054937004786E-4	5.597395290915428E-4	0.0	0.05134078045667391	9.397086984748527E-5
56-57	4.126546893302614E-4	5.883393590451252E-4	0.0	0.057166974387218	9.80565598408542E-5
58-59	4.330831392971061E-4	5.924250490384941E-4	0.0	0.062396657578730225	9.80565598408542E-5
60-61	4.6576865924405743E-4	6.00596429025232E-4	0.0	0.067842882339891	9.80565598408542E-5
62-63	5.474824591114359E-4	6.046821190186009E-4	0.0	0.07327685003107168	9.80565598408542E-5
64-65	5.556538390981738E-4	6.700531589125037E-4	0.0	0.07850244753259053	1.0214224983422313E-4
66-67	6.005964290252319E-4	6.700531589125037E-4	0.0	0.08419381369335344	1.0622793982759205E-4
68-69	6.251105689854455E-4	6.782245388992416E-4	0.0	0.08999140779394395	1.0622793982759205E-4
70-71	6.53710398939028E-4	6.863959188859795E-4	0.0	0.09518023408552248	1.0622793982759205E-4
72-73	7.190814388329308E-4	6.945672988727173E-4	0.0	0.09913926768909698	1.2257069980106775E-4
74-75	7.272528188196687E-4	7.190814388329308E-4	0.0	0.1023833055438319	1.2257069980106775E-4
76-77	7.558526487732512E-4	7.435955787931444E-4	0.0	0.10561508632858671	1.266563897944367E-4
78-79	7.5993833876662E-4	7.517669587798823E-4	0.0	0.10840152690406432	1.307420797878056E-4
80-81	7.64024028759989E-4	7.517669587798823E-4	0.0	0.109627233902075	1.3482776978117453E-4
82-83	7.762810987400957E-4	7.681097187533579E-4	0.0	0.11005623135137874	1.3891345977454345E-4
84-85	7.885381687202026E-4	7.681097187533579E-4	0.0	0.11032180120094771	1.3891345977454345E-4
86-87	7.926238587135715E-4	7.762810987400958E-4	0.0	0.1104280291407753	1.3891345977454345E-4
88-89	7.926238587135715E-4	7.967095487069404E-4	0.0	0.110468886040709	1.3891345977454345E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	16710	0.0	52.488037	1
GTATCAA	31010	0.0	36.829357	1
ATCAACG	38785	0.0	28.586336	3
TCAACGC	38880	0.0	28.516487	4
CAACGCA	39635	0.0	27.973282	5
TATCAAC	40760	0.0	27.621101	2
AACGCAG	40580	0.0	27.374523	6
ACGCAGA	46540	0.0	23.859707	7
CGCAGAG	47300	0.0	23.476336	8
GTACATG	36415	0.0	21.456322	1
TACATGG	36475	0.0	20.987452	2
GCAGAGT	53900	0.0	20.451351	9
ACATGGG	36670	0.0	20.432585	3
GAGTACT	33545	0.0	19.75773	12-13
AGAGTAC	49720	0.0	19.22356	10-11
CATGGGG	27740	0.0	18.535807	4
CAGAGTA	53475	0.0	18.3573	10-11
GTACTTT	37795	0.0	17.429125	14-15
GTGGTAT	8045	0.0	17.42638	1
AGTACTT	36840	0.0	17.369818	12-13
>>END_MODULE