FastQCFastQC Report
Fri 27 May 2016
SRR2139023_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139023_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10657034
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA144850.050.7169571
GTATCAA266300.036.621851
TCAACGC335400.028.6053354
ATCAACG335600.028.4866833
CAACGCA341600.028.0484895
TATCAAC347100.027.752052
AACGCAG351700.027.3402126
ACGCAGA398550.024.0941727
CGCAGAG407150.023.5634778
GCAGAGT467450.020.3685239
GTACATG311750.020.2885531
CTTATAC72700.020.1630171
TACATGG313650.019.6342582
GTGGTAT73250.019.194821
AGAGTAC429000.019.1110710-11
GAGTACT288350.018.98509612-13
ACATGGG318400.018.8313923
TGGTATC71050.018.5861822
CAGAGTA458300.018.30420910-11
AGTACTT318750.017.50290912-13
Produced by FastQC (version 0.11.5)