##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139023_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10657034
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.49552839936515	34.0	31.0	34.0	31.0	34.0
2	32.639938185427575	34.0	31.0	34.0	31.0	34.0
3	32.63292347570628	34.0	31.0	34.0	31.0	34.0
4	36.19646723469213	37.0	35.0	37.0	35.0	37.0
5	36.13196082512264	37.0	35.0	37.0	35.0	37.0
6	36.15773178541046	37.0	36.0	37.0	35.0	37.0
7	36.108158705320825	37.0	36.0	37.0	35.0	37.0
8	36.095013959794066	37.0	36.0	37.0	35.0	37.0
9	37.80048801570869	39.0	38.0	39.0	35.0	39.0
10-11	37.74324159986728	39.0	38.0	39.0	35.0	39.0
12-13	37.7119178281687	39.0	37.5	39.0	35.0	39.0
14-15	39.05207799843747	40.0	38.0	41.0	36.0	41.0
16-17	39.02981458067977	40.0	38.0	41.0	36.0	41.0
18-19	38.9549337085722	40.0	38.5	41.0	35.0	41.0
20-21	39.01508007550807	40.0	39.0	41.0	35.0	41.0
22-23	38.94568197831161	40.0	38.0	41.0	35.0	41.0
24-25	38.86921508397296	40.0	38.0	41.0	35.0	41.0
26-27	38.78847347580068	40.0	38.0	41.0	35.0	41.0
28-29	38.57248794935377	40.0	38.0	41.0	34.5	41.0
30-31	38.49913538090789	40.0	38.0	41.0	34.0	41.0
32-33	38.43006508182889	40.0	38.0	41.0	34.0	41.0
34-35	38.24084827564711	40.0	38.0	41.0	34.0	41.0
36-37	38.361041433534176	40.0	38.0	41.0	34.0	41.0
38-39	38.476821453087965	40.0	38.0	41.0	34.0	41.0
40-41	38.477239772088346	40.0	38.0	41.0	34.0	41.0
42-43	38.352285862000905	40.0	38.0	41.0	34.0	41.0
44-45	38.290335467921395	40.0	38.0	41.0	33.0	41.0
46-47	38.30311605764499	40.0	38.0	41.0	33.0	41.0
48-49	38.18125438869324	40.0	38.0	41.0	33.0	41.0
50-51	38.11911801095138	40.0	37.5	41.0	33.0	41.0
52-53	38.04672500205696	40.0	37.0	41.0	33.0	41.0
54-55	37.93829774325911	40.0	37.0	41.0	33.0	41.0
56-57	37.78500027048941	40.0	36.5	41.0	33.0	41.0
58-59	37.62846590596224	39.5	36.0	41.0	33.0	41.0
60-61	37.44829345129081	39.0	36.0	41.0	32.5	41.0
62-63	37.231692553285825	39.0	35.0	40.5	32.0	41.0
64-65	36.99617590310575	38.5	35.0	40.0	32.0	41.0
66-67	36.7226370873492	38.0	35.0	40.0	31.5	41.0
68-69	36.4165218695156	37.0	35.0	40.0	31.0	41.0
70-71	36.12639017861952	37.0	35.0	39.5	31.0	41.0
72-73	35.80308534770872	36.5	34.5	39.0	31.0	41.0
74-75	35.481507486465986	36.0	34.0	39.0	31.0	40.0
76-77	34.46766323388685	35.0	33.5	37.0	29.5	39.0
78-79	34.78068101983152	35.0	34.0	37.0	30.0	39.0
80-81	34.63002233052451	35.0	34.0	37.0	30.0	39.0
82-83	34.36843931546527	35.0	34.0	36.5	30.0	38.5
84-85	34.10079880296577	35.0	34.0	36.0	30.0	37.0
86-87	33.85193946503772	35.0	34.0	36.0	30.0	37.0
88-89	33.60851006349381	35.0	33.0	35.0	29.5	36.5
90-91	33.48447290844281	35.0	33.0	35.0	29.0	36.0
92-93	33.40689561239742	35.0	33.0	35.0	29.0	36.0
94-95	33.32064146544259	35.0	33.0	35.0	29.0	36.0
96-97	33.3083511343524	35.0	33.0	35.0	30.0	35.0
98-99	33.341413175343106	34.0	33.0	35.0	30.0	35.0
100-101	32.133082197401876	33.0	31.0	34.5	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	5.0
20	64.0
21	341.0
22	1632.0
23	5245.0
24	13267.0
25	27072.0
26	47793.0
27	74349.0
28	105613.0
29	141346.0
30	180683.0
31	226283.0
32	284269.0
33	367586.0
34	510522.0
35	730962.0
36	1209249.0
37	2305029.0
38	3002420.0
39	1364898.0
40	58406.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.628543363941596	21.62542598625471	15.54799393527317	24.198036714530517
2	13.624747748763868	22.097883895275178	41.532343802224894	22.74502455373606
3	16.704385337368002	27.27314185423096	31.611627247074402	24.410845561326635
4	11.863037108504463	19.302707380244644	39.36041020105249	29.473845310198403
5	12.62647214396634	38.34860140165268	36.04403907256748	12.980887381813497
6	28.760639938984795	39.159132349993655	19.06325372881114	13.016973982210406
7	25.396274884711502	33.3939991427025	23.837684380895325	17.37204159169067
8	23.969369535736792	37.18779593302863	21.423740251921465	17.41909427931311
9	25.174711653987035	16.970855179718697	21.660754427048243	36.193678739246025
10-11	23.584924161768956	27.246879195002432	29.440868399870656	19.72732824335795
12-13	24.585068475225672	24.91192785006366	29.477078955256715	21.025924719453954
14-15	21.685749172199994	26.275229573015892	27.226260642933468	24.81276061185065
16-17	21.0932246303732	29.42218874166546	27.80975461839086	21.674832009570487
18-19	21.20898869488134	28.451099560468577	29.67914797282658	20.660763771823504
20-21	21.901715888140576	27.54585215287626	29.667405388367523	20.885026570615643
22-23	21.67732058382593	27.607157281323243	29.456005720254215	21.259516414596614
24-25	21.48251815078723	27.753056359576618	29.515195481786506	21.249230007849647
26-27	21.439127968724137	27.872569912369645	29.492391923721406	21.195910195184812
28-29	21.37014453185272	27.811895403743385	29.459890673567195	21.358069390836697
30-31	21.644134931548994	27.796877014989697	29.378215584653795	21.18077246880752
32-33	21.464146247911177	27.844027695062845	29.356915938015238	21.334910119010743
34-35	21.528230673084288	27.974486730310836	29.390954964537762	21.106327632067114
36-37	21.60949202505299	27.917876583562638	29.210725635833377	21.261905755550995
38-39	21.441143120770576	27.947235938131943	29.295252922478554	21.316368018618924
40-41	21.588937589829023	27.871773613425177	29.16799697769987	21.37129181904593
42-43	21.582459521196085	28.016277972716168	28.91110785044657	21.49015465564117
44-45	21.639154637778727	28.105607035354986	28.72933913939326	21.525899187473026
46-47	21.71141747151944	28.098902698177415	28.377255847005518	21.812423983297627
48-49	21.770587805631923	28.221869105718078	28.246588145568474	21.760954943081526
50-51	21.764384650999816	28.295771919162938	28.144533730506694	21.795309699330552
52-53	21.813408193814755	28.309993788312678	28.096662652792464	21.779935365080103
54-55	21.802850015848595	28.23992157073346	28.07898484623756	21.878243567180384
56-57	21.880514787768448	28.356398709326115	27.95117363906335	21.811912863842085
58-59	21.724093554879236	28.450836337631515	28.10129789101974	21.723772216469516
60-61	21.89249918853627	28.48305935134881	27.943239216788335	21.681202243326585
62-63	21.842194982391277	28.575304301111178	27.893274597678648	21.6892261188189
64-65	21.798938684329215	28.47444840634993	27.90870505160158	21.817907857719277
66-67	21.86725431836658	28.43213899396	27.839829079353134	21.86077760832029
68-69	21.82643159444351	28.348761631222956	27.878348951675015	21.94645782265852
70-71	21.773808910767453	28.238757864105647	27.94174544413966	22.045687780987244
72-73	21.91481928365347	28.266340347305736	27.878293421952566	21.94054694708823
74-75	21.78908028753036	28.390989626899387	27.848392370694093	21.971537714876163
76-77	21.82057630606701	28.35808573496625	27.81323963707646	22.008098321890284
78-79	21.776070071609478	28.29317292675783	27.839462130749176	22.091294870883516
80-81	21.833156645570075	28.33213807844429	27.80288232837229	22.031822947613342
82-83	21.855225304609753	28.31922618198619	27.917307271053023	21.908241242351032
84-85	21.91312359339254	28.310609248363484	27.838502955250892	21.937764202993083
86-87	21.825114499958016	28.34139318582239	27.86501749883905	21.96847481538055
88-89	21.82755270177119	28.261167115649027	27.84347120583717	22.067808976742615
90-91	21.87331920202403	28.35720160878893	27.83915907889668	21.930320110290356
92-93	21.87602276591883	28.286181267722377	27.904468793052484	21.933327173306314
94-95	21.70668301283852	28.390699600583563	27.93244704591553	21.97017034066239
96-97	21.694407109255014	28.430089283979065	27.952735130287476	21.922768476478442
98-99	21.920148452112393	28.989915077793892	27.988294647919847	21.10164182217386
100-101	22.366212381825402	31.72624445661713	25.21718437570502	20.690358785852446
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4479.0
1	3700.5
2	4047.5
3	5433.0
4	6886.5
5	9761.5
6	12198.5
7	14684.0
8	16729.0
9	17107.0
10	15883.5
11	14476.0
12	15994.0
13	21759.0
14	35353.5
15	53681.5
16	68067.0
17	75070.0
18	75303.5
19	71458.0
20	65819.5
21	60761.0
22	58749.0
23	63005.0
24	73342.5
25	88467.0
26	107640.5
27	128892.5
28	154244.5
29	182545.5
30	208962.0
31	233961.0
32	258811.5
33	282343.5
34	304395.5
35	324695.5
36	344877.0
37	363314.5
38	377899.5
39	389744.5
40	399280.5
41	407844.5
42	412336.0
43	414395.5
44	418500.0
45	424060.5
46	429455.5
47	431390.5
48	426389.5
49	416330.5
50	405425.5
51	389845.5
52	368483.5
53	345615.5
54	322969.5
55	298224.0
56	272069.0
57	244799.5
58	215511.5
59	185033.0
60	153412.0
61	124569.5
62	98976.0
63	76041.0
64	57119.5
65	42910.0
66	32827.0
67	25191.5
68	19051.5
69	13928.5
70	10228.0
71	7517.5
72	5266.5
73	3725.5
74	2532.5
75	1703.0
76	1116.0
77	667.0
78	434.5
79	297.0
80	198.5
81	135.5
82	98.5
83	67.5
84	47.5
85	34.0
86	26.5
87	20.5
88	14.0
89	10.5
90	8.5
91	5.5
92	3.5
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	6.193092749821386E-4
4	1.501355818138518E-4
5	4.0348937612472667E-4
6	1.6890252954058322E-4
7	0.0027212074203760636
8	5.817753795286756E-4
9	4.316397977148238E-4
10-11	7.50677909069259E-5
12-13	4.2225632385145805E-5
14-15	2.0174468806236334E-4
16-17	9.618060709949879E-4
18-19	2.8150421590097207E-5
20-21	0.0
22-23	0.0029092190853593504
24-25	0.0071619863914749275
26-27	0.035744453963674004
28-29	0.27973265261499597
30-31	0.24379510392865197
32-33	0.251280334817966
34-35	0.13196822306160888
36-37	0.023263543336217107
38-39	0.01495435342325579
40-41	0.008401511349750115
42-43	0.005621185775110483
44-45	0.006314072095382891
46-47	0.0013651340236344618
48-49	0.0019006629930466582
50-51	0.0024034953948225395
52-53	0.0013196817231058977
54-55	1.6814104282093795E-4
56-57	0.0014180544118781169
58-59	0.0027779452088659783
60-61	0.011816924879547105
62-63	0.007643111325476911
64-65	0.002171508047406575
66-67	0.0015801847837987415
68-69	0.002209888954437465
70-71	0.003833226672018926
72-73	0.003272611168275673
74-75	0.0034050254261467593
76-77	0.001462523842950237
78-79	6.275624157889684E-5
80-81	3.403191432171395E-4
82-83	1.1667238458578125E-5
84-85	0.0
86-87	1.1936247309495256E-5
88-89	0.0
90-91	3.6746985369187776E-4
92-93	0.007251072904039165
94-95	0.0010921177318065655
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	202.0
20-21	736.0
22-23	1587.0
24-25	4058.0
26-27	5847.0
28-29	9792.0
30-31	20940.0
32-33	27717.0
34-35	39884.0
36-37	54653.0
38-39	65808.0
40-41	70458.0
42-43	84006.0
44-45	100188.0
46-47	99598.0
48-49	87209.0
50-51	77209.0
52-53	74534.0
54-55	79901.0
56-57	84248.0
58-59	84616.0
60-61	84356.0
62-63	83594.0
64-65	88802.0
66-67	95259.0
68-69	91642.0
70-71	89218.0
72-73	86498.0
74-75	88109.0
76-77	88805.0
78-79	94767.0
80-81	97994.0
82-83	95116.0
84-85	96729.0
86-87	102153.0
88-89	108486.0
90-91	115281.0
92-93	123523.0
94-95	140035.0
96-97	216456.0
98-99	908524.0
100-101	6688496.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.33958141514756
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.82812112404709	74.02898432372112
2	7.487713077173545	12.480457472167398
3	1.9723823470378963	4.931325575782938
4	0.7769288264299219	2.589956927361261
5	0.3551258670322271	1.479802055407858
6	0.19557771528103748	0.9779618955391542
7	0.11932783403626344	0.6961312217830876
8	0.0690115312534524	0.4601113701984878
9	0.04531736217523037	0.33990569950701066
>10	0.14784418113725767	1.8353955334730918
>50	0.00238030979645501	0.12961645905245803
>100	2.5052094252821586E-4	0.042162884191997395
>500	1.930365714362543E-5	0.008188581814208144
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	9.383473863365735E-6	0.0	0.0	0.0
3	0.0	9.383473863365735E-6	0.0	0.0	0.0
4	0.0	3.753389545346294E-5	0.0	0.0	0.0
5	0.0	4.691736931682868E-5	0.0	0.0	0.0
6	0.0	4.691736931682868E-5	0.0	0.0	0.0
7	0.0	4.691736931682868E-5	0.0	0.0	0.0
8	0.0	4.691736931682868E-5	0.0	0.0	0.0
9	0.0	5.6300843180194414E-5	0.0	9.383473863365735E-6	0.0
10-11	4.6917369316828675E-6	5.6300843180194414E-5	0.0	9.383473863365735E-6	0.0
12-13	9.383473863365735E-6	5.6300843180194414E-5	0.0	9.383473863365735E-6	0.0
14-15	9.383473863365735E-6	7.975952783860875E-5	0.0	9.383473863365735E-6	0.0
16-17	2.345868465841434E-5	8.445126477029162E-5	0.0	9.383473863365735E-6	0.0
18-19	3.753389545346294E-5	8.445126477029162E-5	0.0	9.383473863365735E-6	0.0
20-21	3.753389545346294E-5	8.445126477029162E-5	0.0	9.383473863365735E-6	0.0
22-23	1.032182124970231E-4	8.445126477029162E-5	0.0	9.383473863365735E-6	0.0
24-25	1.032182124970231E-4	9.852647556534023E-5	0.0	3.753389545346294E-5	0.0
26-27	1.1260168636038883E-4	1.1260168636038883E-4	0.0	9.852647556534023E-5	0.0
28-29	1.2198516022375457E-4	1.1260168636038883E-4	0.0	3.002711636277036E-4	0.0
30-31	1.313686340871203E-4	1.2198516022375457E-4	0.0	7.647531198643075E-4	0.0
32-33	1.4075210795048604E-4	1.2198516022375457E-4	0.0	0.001961146037443439	0.0
34-35	1.4075210795048604E-4	1.3606037101880316E-4	0.0	0.0037017804390977824	0.0
36-37	1.876694772673147E-4	1.5013558181385176E-4	0.0	0.006061724115734265	0.0
38-39	1.876694772673147E-4	1.5482731874553465E-4	0.0	0.009786963239490461	0.0
40-41	1.9236121419899757E-4	1.78286003403949E-4	0.0	0.013648262734265462	0.0
42-43	1.9705295113068045E-4	1.876694772673147E-4	0.0	0.01752363743983551	0.0
44-45	1.9705295113068045E-4	2.064364249940462E-4	0.0	0.022360818216400548	0.0
46-47	1.9705295113068045E-4	2.158198988574119E-4	0.0	0.02794398516510316	0.0
48-49	1.9705295113068045E-4	2.158198988574119E-4	9.383473863365735E-6	0.033086128842227586	0.0
50-51	2.0174468806236334E-4	2.908876897643378E-4	9.383473863365735E-6	0.03830803204719062	0.0
52-53	2.064364249940462E-4	3.096546374910693E-4	9.383473863365735E-6	0.04322028061466258	4.6917369316828675E-6
54-55	2.1112816192572905E-4	3.096546374910693E-4	9.383473863365735E-6	0.04842810860883056	9.383473863365735E-6
56-57	2.2989510965246054E-4	3.096546374910693E-4	9.383473863365735E-6	0.053804839132539126	9.383473863365735E-6
58-59	2.721207420376063E-4	3.096546374910693E-4	9.383473863365735E-6	0.059036125811365525	9.383473863365735E-6
60-61	2.908876897643378E-4	3.096546374910693E-4	9.383473863365735E-6	0.06417826948848995	9.383473863365735E-6
62-63	2.908876897643378E-4	3.096546374910693E-4	9.383473863365735E-6	0.06914212716221042	9.383473863365735E-6
64-65	2.908876897643378E-4	3.941059022613609E-4	9.383473863365735E-6	0.07424673694388138	9.383473863365735E-6
66-67	2.908876897643378E-4	3.941059022613609E-4	9.383473863365735E-6	0.07961877573065826	9.383473863365735E-6
68-69	2.908876897643378E-4	3.9879763919304376E-4	9.383473863365735E-6	0.08523009310095098	9.383473863365735E-6
70-71	2.908876897643378E-4	4.034893761247266E-4	9.383473863365735E-6	0.09007196561444769	9.383473863365735E-6
72-73	2.908876897643378E-4	4.034893761247266E-4	9.383473863365735E-6	0.09426637843137217	9.383473863365735E-6
74-75	2.908876897643378E-4	4.2694806078314096E-4	9.383473863365735E-6	0.09728316527844427	9.383473863365735E-6
76-77	2.908876897643378E-4	4.316397977148238E-4	9.383473863365735E-6	0.10013104959597577	9.383473863365735E-6
78-79	2.908876897643378E-4	4.316397977148238E-4	9.383473863365735E-6	0.1029132495964637	9.383473863365735E-6
80-81	2.908876897643378E-4	4.316397977148238E-4	9.383473863365735E-6	0.10415655988335967	9.383473863365735E-6
82-83	2.908876897643378E-4	4.316397977148238E-4	9.383473863365735E-6	0.10459289141800618	9.383473863365735E-6
84-85	2.908876897643378E-4	4.316397977148238E-4	9.383473863365735E-6	0.10484624521231704	9.383473863365735E-6
86-87	2.908876897643378E-4	4.316397977148238E-4	9.383473863365735E-6	0.1048884708447022	9.383473863365735E-6
88-89	2.908876897643378E-4	4.410232715781896E-4	9.383473863365735E-6	0.1049166212662923	9.383473863365735E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14485	0.0	50.716957	1
GTATCAA	26630	0.0	36.62185	1
TCAACGC	33540	0.0	28.605335	4
ATCAACG	33560	0.0	28.486683	3
CAACGCA	34160	0.0	28.048489	5
TATCAAC	34710	0.0	27.75205	2
AACGCAG	35170	0.0	27.340212	6
ACGCAGA	39855	0.0	24.094172	7
CGCAGAG	40715	0.0	23.563477	8
GCAGAGT	46745	0.0	20.368523	9
GTACATG	31175	0.0	20.288553	1
CTTATAC	7270	0.0	20.163017	1
TACATGG	31365	0.0	19.634258	2
GTGGTAT	7325	0.0	19.19482	1
AGAGTAC	42900	0.0	19.11107	10-11
GAGTACT	28835	0.0	18.985096	12-13
ACATGGG	31840	0.0	18.831392	3
TGGTATC	7105	0.0	18.586182	2
CAGAGTA	45830	0.0	18.304209	10-11
AGTACTT	31875	0.0	17.502909	12-13
>>END_MODULE