FastQCFastQC Report
Fri 27 May 2016
SRR2139022_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139022_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9879421
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA137250.050.119331
GTATCAA254250.038.5671541
ATCAACG325100.029.5213573
TCAACGC326200.029.4349984
TATCAAC333350.029.0585922
CAACGCA331550.028.9215325
AACGCAG338350.028.4030046
CTTATAC73150.028.3800471
CGCAGAG389650.024.6412268
ACGCAGA390300.024.6115867
TATACAC116550.022.046463
GCAGAGT448150.021.253679
AGAGTAC410500.019.70555110-11
GAGTACT273150.019.47556712-13
TACATGG291900.019.4271032
GTACATG299650.019.3929581
ACATGGG294000.018.6951713
TTATACA122150.018.5628972
CAGAGTA441200.018.44044510-11
AGTACTT299650.018.03715312-13
Produced by FastQC (version 0.11.5)