##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139022_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9879421
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.49619000951574	34.0	31.0	34.0	31.0	34.0
2	32.63562257342814	34.0	31.0	34.0	31.0	34.0
3	32.62922877767837	34.0	31.0	34.0	31.0	34.0
4	36.1910858946086	37.0	35.0	37.0	35.0	37.0
5	36.12822795991789	37.0	35.0	37.0	35.0	37.0
6	36.152334635805076	37.0	36.0	37.0	35.0	37.0
7	36.10016194268875	37.0	36.0	37.0	35.0	37.0
8	36.08849091459914	37.0	36.0	37.0	35.0	37.0
9	37.79184680964603	39.0	38.0	39.0	35.0	39.0
10-11	37.73467630339875	39.0	37.5	39.0	35.0	39.0
12-13	37.70214499412465	39.0	37.0	39.0	35.0	39.0
14-15	39.039988224006244	40.0	38.0	41.0	36.0	41.0
16-17	39.01576301890566	40.0	38.0	41.0	36.0	41.0
18-19	38.942284421323876	40.0	38.5	41.0	35.0	41.0
20-21	39.005134056743344	40.0	38.5	41.0	35.0	41.0
22-23	38.93624566599473	40.0	38.0	41.0	35.0	41.0
24-25	38.85719603338515	40.0	38.0	41.0	35.0	41.0
26-27	38.77507497720318	40.0	38.0	41.0	35.0	41.0
28-29	38.55673311529798	40.0	38.0	41.0	34.5	41.0
30-31	38.48082086470479	40.0	38.0	41.0	34.0	41.0
32-33	38.40851471430747	40.0	38.0	41.0	34.0	41.0
34-35	38.218527141098626	40.0	38.0	41.0	34.0	41.0
36-37	38.342431736526834	40.0	38.0	41.0	34.0	41.0
38-39	38.45480698471936	40.0	38.0	41.0	34.0	41.0
40-41	38.46129909211958	40.0	38.0	41.0	34.0	41.0
42-43	38.34019834066844	40.0	38.0	41.0	34.0	41.0
44-45	38.27900047152674	40.0	38.0	41.0	33.0	41.0
46-47	38.29212914057659	40.0	38.0	41.0	33.0	41.0
48-49	38.168642896360154	40.0	38.0	41.0	33.0	41.0
50-51	38.10598497783658	40.0	37.5	41.0	33.0	41.0
52-53	38.03568233119587	40.0	37.0	41.0	33.0	41.0
54-55	37.92511040622128	40.0	37.0	41.0	33.0	41.0
56-57	37.76478960785796	40.0	36.5	41.0	33.0	41.0
58-59	37.60752422047075	39.0	36.0	41.0	33.0	41.0
60-61	37.42247225520093	39.0	36.0	41.0	32.0	41.0
62-63	37.20825864881904	39.0	35.0	40.5	32.0	41.0
64-65	36.976104521017774	38.5	35.0	40.0	32.0	41.0
66-67	36.70538843676548	38.0	35.0	40.0	31.0	41.0
68-69	36.40000554073453	37.0	35.0	40.0	31.0	41.0
70-71	36.108430756847355	37.0	35.0	39.5	31.0	41.0
72-73	35.78696281834724	36.0	34.5	39.0	31.0	41.0
74-75	35.4651375687873	36.0	34.0	39.0	31.0	40.0
76-77	34.44805667958437	35.0	33.5	37.0	29.5	39.0
78-79	34.76243987669811	35.0	34.0	37.0	30.0	39.0
80-81	34.61571290147208	35.0	34.0	37.0	30.0	39.0
82-83	34.35455999848767	35.0	34.0	36.0	30.0	38.5
84-85	34.08887699086428	35.0	34.0	36.0	30.0	37.0
86-87	33.84061227269072	35.0	34.0	35.5	30.0	37.0
88-89	33.598879031471824	35.0	33.0	35.0	29.5	36.5
90-91	33.473877682860945	35.0	33.0	35.0	29.0	36.0
92-93	33.39810686368503	35.0	33.0	35.0	29.0	36.0
94-95	33.31495696849157	35.0	33.0	35.0	29.0	36.0
96-97	33.302986857505275	35.0	33.0	35.0	29.5	35.0
98-99	33.33936097159011	34.0	33.0	35.0	30.0	35.0
100-101	32.13004535151811	33.0	31.0	34.5	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	3.0
19	11.0
20	62.0
21	322.0
22	1489.0
23	5003.0
24	12275.0
25	25002.0
26	44231.0
27	69184.0
28	98735.0
29	132215.0
30	168315.0
31	210646.0
32	265053.0
33	344240.0
34	473321.0
35	678673.0
36	1122571.0
37	2136701.0
38	2757544.0
39	1277752.0
40	56072.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.50462491678409	21.635741608744073	15.495675303238924	24.36395817123291
2	13.643167954883188	21.99394073802503	41.458704918031124	22.904186389060655
3	16.644772549169655	27.317927081555638	31.652709293288662	24.38459107598605
4	11.877510399920967	19.29655256461879	39.24696049416756	29.578976541292683
5	12.597368274282184	38.3679207172358	36.066185576756745	12.968525431725272
6	28.69968814929884	39.12587061146483	19.020554573771484	13.153886665464839
7	25.328611466742462	33.4135427117977	23.733351796471435	17.5244940249884
8	24.081031895862985	37.01741477618662	21.45954868170224	17.442004646248154
9	25.31901004829144	16.818141927979163	21.54440491321783	36.318443110511566
10-11	23.551486487683352	27.230187017699688	29.381676288949365	19.836650205667596
12-13	24.614134697597095	24.796372134839103	29.421952849656442	21.167540317907356
14-15	21.778648072839992	26.108694974435714	27.179138669037663	24.933518283686634
16-17	21.134194573532852	29.325994028535774	27.76199958721851	21.777811810712862
18-19	21.227589395692195	28.364370414899902	29.683683827965996	20.724356361441902
20-21	22.022393956368873	27.376761472256465	29.651099584638025	20.949744986736636
22-23	21.721174973253543	27.473196851490556	29.43408061434647	21.371547560909438
24-25	21.50978567697314	27.63709383138075	29.571576223177342	21.281544268468767
26-27	21.53713550121468	27.80427860171177	29.440612150116586	21.217973746956968
28-29	21.401074594653497	27.719532787761764	29.481245519811633	21.39814709777311
30-31	21.680048913659963	27.721257247904664	29.38708884879443	21.211604989640946
32-33	21.537024262235978	27.691866768650584	29.36633278555379	21.40477618355964
34-35	21.508132444676626	27.825627803383302	29.47732819810069	21.188911553839386
36-37	21.68501113658876	27.793986247192677	29.23282193798108	21.288180678237488
38-39	21.497394434884825	27.775187623095665	29.319173935540434	21.40824400647908
40-41	21.619036820568766	27.735827761210853	29.195785805491386	21.449349612728998
42-43	21.67721193455929	27.86501012753012	28.934956662316974	21.522821275593618
44-45	21.723864594604304	28.07068036816481	28.684369159048373	21.521085878182518
46-47	21.83145281919567	27.913129114561702	28.342117132917572	21.913300933325058
48-49	21.843168666887337	28.092877736612447	28.19980569889004	21.864147897610174
50-51	21.739292372259722	28.145549809863006	28.223987892300194	21.891169925577074
52-53	21.805939854825308	28.124910865458286	28.212266814476557	21.856882465239853
54-55	21.838167772776895	28.080398890341012	28.11210091510131	21.969332421780788
56-57	22.06420331611193	28.176012889305884	27.892434382541214	21.86734941204097
58-59	21.76804514214111	28.441189294108277	28.12525350855845	21.665512055192167
60-61	22.02054065659185	28.468852288139146	27.854102746968906	21.656504308300097
62-63	21.853408360791608	28.52937778039498	27.836217782659077	21.78099607615433
64-65	21.84582728570817	28.411440217012036	27.889026909764375	21.853705587515417
66-67	22.00516672948006	28.352001007346107	27.739927486237164	21.902904776936673
68-69	21.92713013820829	28.206282818729832	27.90988685758454	21.95670018547734
70-71	21.906185181783737	28.067519758221245	27.985204010952565	22.04109104904246
72-73	21.991939679839742	28.06225624468449	27.930460516151207	22.015343559324563
74-75	21.90143611670387	28.17805304252825	27.87736291690633	22.043147923861554
76-77	21.98387811926949	28.146069692804847	27.79241021257153	22.07764197535413
78-79	21.902413938198325	28.09429584602188	27.843283421794613	22.160006793985186
80-81	21.930705158623784	28.08857560757333	27.870732148556783	22.109987085246104
82-83	21.94471777871799	28.061766210783855	27.96393770871632	22.029578301781836
84-85	22.103049283744	28.04368535695335	27.855124351700994	21.998141007601653
86-87	21.941284567531742	28.067834676246665	27.85975839797083	22.13112235825076
88-89	22.02027007998244	27.919806900865606	27.88748916448956	22.17243385466239
90-91	22.0444866824002	27.981402871291987	27.930814549581306	22.0432958967265
92-93	22.108699783055886	27.949023126461377	27.860405739096798	22.081871351385946
94-95	21.91145035045946	27.95692959681238	27.97467053190612	22.156949520822042
96-97	21.86432943487049	27.99835108119234	28.07535088314454	22.06196860079263
98-99	22.091752347335728	28.565253784477058	28.069063452022714	21.273930416164507
100-101	22.622435253868833	31.291696184504698	25.23132177875952	20.854546782866947
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2611.0
1	2366.0
2	3240.0
3	4602.0
4	6060.0
5	8884.0
6	11209.0
7	13820.0
8	16193.5
9	16480.0
10	14966.0
11	13419.0
12	14830.0
13	20404.0
14	33385.0
15	50962.5
16	64835.0
17	71525.5
18	71836.5
19	67950.5
20	61733.5
21	55739.5
22	52918.0
23	56188.0
24	65557.5
25	80911.0
26	100855.5
27	121225.5
28	143210.5
29	167798.5
30	189839.5
31	211153.5
32	235117.5
33	258714.0
34	280228.0
35	298289.5
36	317241.0
37	334995.5
38	346418.5
39	353593.5
40	363758.0
41	374445.0
42	378581.5
43	383217.0
44	391038.5
45	398984.5
46	407213.5
47	411388.5
48	404921.5
49	395142.5
50	384875.5
51	368877.0
52	348349.0
53	328767.5
54	308821.0
55	282861.0
56	255871.5
57	228322.0
58	200765.5
59	174116.5
60	146142.0
61	118766.5
62	92218.5
63	69753.5
64	52384.5
65	39901.5
66	30910.0
67	23561.5
68	17874.0
69	12750.0
70	8974.0
71	6403.5
72	4427.0
73	3104.5
74	2161.0
75	1487.5
76	982.5
77	620.0
78	402.5
79	272.0
80	198.5
81	146.0
82	99.5
83	65.5
84	46.0
85	33.5
86	28.0
87	27.0
88	18.0
89	10.5
90	9.5
91	8.0
92	6.0
93	3.5
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	5.567127871157632E-4
4	1.9231896282180907E-4
5	4.8585843239193875E-4
6	1.417087094476488E-4
7	0.00280380803692848
8	5.26346635091267E-4
9	3.036615202449617E-4
10-11	8.603743073607249E-5
12-13	2.0244101349664116E-5
14-15	1.3158665877281676E-4
16-17	0.0010324491688328697
18-19	2.0244101349664116E-5
20-21	0.0
22-23	0.002819333134073463
24-25	0.007057679314544414
26-27	0.035070661507436714
28-29	0.27798277770820085
30-31	0.24202781310394683
32-33	0.249607810732129
34-35	0.1298664936337419
36-37	0.021340935971353673
38-39	0.012356009228429804
40-41	0.0059669514870773245
42-43	0.003091163960035845
44-45	0.004135482189584517
46-47	7.558265175878702E-4
48-49	0.0012362704108244828
50-51	0.001962539990818349
52-53	9.616875693466896E-4
54-55	4.956881192039601E-5
56-57	0.0011777207891773681
58-59	0.002152761648416686
60-61	0.00960772148471549
62-63	0.005180197185605151
64-65	9.631506075144548E-4
66-67	0.0018712502854968311
68-69	0.002504874485749268
70-71	0.004258248540090755
72-73	0.003844345518224093
74-75	0.003968526726690481
76-77	0.00161510976328939
78-79	5.587155749726462E-5
80-81	4.583909723351629E-4
82-83	0.0
84-85	0.0
86-87	1.30181907084226E-5
88-89	0.0
90-91	4.816637951922728E-4
92-93	0.008077090084621274
94-95	0.0011467918429110715
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	200.0
20-21	686.0
22-23	1533.0
24-25	4276.0
26-27	5615.0
28-29	9198.0
30-31	19592.0
32-33	26013.0
34-35	37445.0
36-37	51573.0
38-39	62376.0
40-41	66803.0
42-43	79850.0
44-45	96035.0
46-47	94896.0
48-49	81992.0
50-51	73358.0
52-53	70920.0
54-55	76217.0
56-57	81483.0
58-59	82003.0
60-61	82709.0
62-63	83709.0
64-65	88701.0
66-67	96195.0
68-69	88799.0
70-71	85677.0
72-73	83191.0
74-75	85264.0
76-77	86369.0
78-79	90791.0
80-81	93541.0
82-83	92442.0
84-85	94228.0
86-87	99073.0
88-89	105063.0
90-91	112493.0
92-93	119122.0
94-95	133030.0
96-97	198013.0
98-99	839266.0
100-101	6099681.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.14632233896822
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.5353004205669	72.72849326725276
2	7.501567796572081	12.324524125296675
3	2.0254210576180873	4.99142673213688
4	0.837563551940859	2.752110620684198
5	0.39101013781823735	1.6060022409510655
6	0.22973221234166186	1.132299381999747
7	0.12971451607279905	0.7458899314551597
8	0.09573674742364506	0.6291537370837705
9	0.05875373623498539	0.4343763019840044
>10	0.1920340293907731	2.420607731745284
>50	0.0026050180222445523	0.13395945861597874
>100	5.152121117992736E-4	0.07411423057792471
>500	4.556388599854532E-5	0.027042240216493582
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.0366152024496172E-5	0.0	1.0122050674832058E-5	0.0
3	1.0122050674832058E-5	4.048820269932823E-5	0.0	1.0122050674832058E-5	0.0
4	1.0122050674832058E-5	1.6195281079731293E-4	0.0	1.0122050674832058E-5	0.0
5	4.048820269932823E-5	1.6195281079731293E-4	0.0	1.0122050674832058E-5	0.0
6	4.048820269932823E-5	1.6195281079731293E-4	0.0	1.0122050674832058E-5	0.0
7	4.048820269932823E-5	1.7207486147214498E-4	0.0	2.0244101349664116E-5	0.0
8	4.048820269932823E-5	2.0244101349664115E-4	0.0	3.0366152024496172E-5	0.0
9	4.048820269932823E-5	3.643938242939541E-4	0.0	5.061025337416029E-5	0.0
10-11	4.048820269932823E-5	3.643938242939541E-4	0.0	5.061025337416029E-5	0.0
12-13	4.048820269932823E-5	3.643938242939541E-4	0.0	5.061025337416029E-5	0.0
14-15	4.048820269932823E-5	4.3524817901777847E-4	0.0	5.5671278711576316E-5	1.0122050674832058E-5
16-17	4.048820269932823E-5	4.3524817901777847E-4	0.0	6.0732304048992344E-5	2.0244101349664116E-5
18-19	4.048820269932823E-5	4.7067535637969067E-4	0.0	6.0732304048992344E-5	2.0244101349664116E-5
20-21	4.554922803674426E-5	5.21285609753851E-4	0.0	8.097640539865646E-5	2.0244101349664116E-5
22-23	6.0732304048992344E-5	5.820179138028433E-4	0.0	9.109845607348852E-5	2.0244101349664116E-5
24-25	7.085435472382441E-5	6.376891925144196E-4	0.0	1.062815320857366E-4	2.0244101349664116E-5
26-27	7.085435472382441E-5	6.781773952137479E-4	0.0	1.3664768411023279E-4	2.0244101349664116E-5
28-29	8.097640539865646E-5	6.832384205511638E-4	0.0	3.49210748281706E-4	2.0244101349664116E-5
30-31	9.109845607348852E-5	7.996420033117325E-4	0.0	9.666558394464615E-4	2.0244101349664116E-5
32-33	9.109845607348852E-5	7.996420033117325E-4	0.0	0.002211668072450805	3.0366152024496172E-5
34-35	9.109845607348852E-5	8.958014847226371E-4	0.0	0.0038818064337980936	4.048820269932823E-5
36-37	9.109845607348852E-5	0.0010071440421457896	0.0	0.006498356533242182	4.048820269932823E-5
38-39	9.109845607348852E-5	0.0010628153208573662	0.0	0.01028400348562937	4.048820269932823E-5
40-41	9.109845607348852E-5	0.0011640358276056866	0.0	0.014469471439672426	4.554922803674426E-5
42-43	9.109845607348852E-5	0.0011944019796301828	0.0	0.01854359683629233	5.5671278711576316E-5
44-45	9.109845607348852E-5	0.0012956224863785034	0.0	0.023199740146715074	6.0732304048992344E-5
46-47	9.109845607348852E-5	0.001391781967789408	0.0	0.028559365979038652	7.085435472382441E-5
48-49	1.0122050674832058E-4	0.001396842993126824	0.0	0.03393417488737447	1.0122050674832058E-4
50-51	1.0122050674832058E-4	0.0015942229812860491	0.0	0.03913184790890073	1.0122050674832058E-4
52-53	1.0122050674832058E-4	0.001609406057298297	0.0	0.04420299529699159	1.3158665877281676E-4
54-55	1.214646080979847E-4	0.0016296501586479614	0.0	0.04965878061072607	1.5183076012248087E-4
56-57	1.4676973478506484E-4	0.0016802604120221215	0.0	0.05576743819298722	1.6195281079731293E-4
58-59	1.5183076012248087E-4	0.0016903824626969535	0.0	0.06118273530402237	1.6195281079731293E-4
60-61	1.6195281079731293E-4	0.0017055655387092018	0.0	0.06661321549106977	1.6195281079731293E-4
62-63	1.6195281079731293E-4	0.0017308706653962817	0.0	0.07176533928455929	1.6195281079731293E-4
64-65	1.6195281079731293E-4	0.001933311678892923	0.0	0.07739319945976592	1.7207486147214498E-4
66-67	1.6195281079731293E-4	0.0019687388562548355	0.0	0.0829147781028868	1.8219691214697704E-4
68-69	1.6195281079731293E-4	0.0020193491096289953	0.0	0.08837056341662128	1.8219691214697704E-4
70-71	1.6195281079731293E-4	0.0020345321856412436	0.0	0.09353280926078562	1.8219691214697704E-4
72-73	1.6195281079731293E-4	0.0020497152616534915	0.0	0.09797132848169948	1.923189628218091E-4
74-75	1.6701383613472895E-4	0.002125630641714732	0.0	0.10132172725506888	1.923189628218091E-4
76-77	1.77135886809561E-4	0.0022319121738004688	0.0	0.10417614554537154	1.923189628218091E-4
78-79	1.8219691214697704E-4	0.002267339351162381	0.0	0.1070052587089871	1.923189628218091E-4
80-81	1.8219691214697704E-4	0.002277461401837213	0.0	0.10821990478996694	2.0244101349664115E-4
82-83	1.8219691214697704E-4	0.0022977055031868773	0.0	0.10866021399432213	2.2268511484630526E-4
84-85	1.8219691214697704E-4	0.002328071655211373	0.0	0.10901448576794126	2.2268511484630526E-4
86-87	1.8219691214697704E-4	0.002338193705886205	0.0	0.10911064524935216	2.277461401837213E-4
88-89	1.8219691214697704E-4	0.0024596583139841898	0.0	0.1091561944773889	2.3280716552113732E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13725	0.0	50.11933	1
GTATCAA	25425	0.0	38.567154	1
ATCAACG	32510	0.0	29.521357	3
TCAACGC	32620	0.0	29.434998	4
TATCAAC	33335	0.0	29.058592	2
CAACGCA	33155	0.0	28.921532	5
AACGCAG	33835	0.0	28.403004	6
CTTATAC	7315	0.0	28.380047	1
CGCAGAG	38965	0.0	24.641226	8
ACGCAGA	39030	0.0	24.611586	7
TATACAC	11655	0.0	22.04646	3
GCAGAGT	44815	0.0	21.25367	9
AGAGTAC	41050	0.0	19.705551	10-11
GAGTACT	27315	0.0	19.475567	12-13
TACATGG	29190	0.0	19.427103	2
GTACATG	29965	0.0	19.392958	1
ACATGGG	29400	0.0	18.695171	3
TTATACA	12215	0.0	18.562897	2
CAGAGTA	44120	0.0	18.440445	10-11
AGTACTT	29965	0.0	18.037153	12-13
>>END_MODULE