FastQCFastQC Report
Fri 27 May 2016
SRR2139021_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139021_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10621316
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA141850.048.0144581
GTATCAA253300.035.907181
CTTATAC96700.034.118631
ATCAACG313600.028.4613633
TCAACGC315400.028.2989354
CAACGCA322550.027.697965
AACGCAG329450.027.233856
TATCAAC332250.027.1575742
TATACAC140050.026.6201483
TTATACA149300.023.7478182
ACGCAGA379100.023.5997717
CGCAGAG384150.023.277938
ACATCTC165350.020.337988
GCAGAGT446550.019.8444569
ACACATC170050.019.7764176
TACACAT191100.019.4644665
GTACATG303450.019.2543261
TACATGG302250.018.8671682
GAGTACT268700.018.70995912-13
AGAGTAC409750.018.5231110-11
Produced by FastQC (version 0.11.5)