##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139021_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10621316
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.501867565186835	34.0	31.0	34.0	31.0	34.0
2	32.64590103523895	34.0	31.0	34.0	31.0	34.0
3	32.6396131138552	34.0	31.0	34.0	31.0	34.0
4	36.1981426783649	37.0	35.0	37.0	35.0	37.0
5	36.13677928422429	37.0	35.0	37.0	35.0	37.0
6	36.16164211666426	37.0	36.0	37.0	35.0	37.0
7	36.10927205254038	37.0	36.0	37.0	35.0	37.0
8	36.09462490335473	37.0	36.0	37.0	35.0	37.0
9	37.801635691848354	39.0	38.0	39.0	35.0	39.0
10-11	37.743057875314136	39.0	37.5	39.0	35.0	39.0
12-13	37.71553699183792	39.0	37.5	39.0	35.0	39.0
14-15	39.058724973440206	40.0	38.0	41.0	36.0	41.0
16-17	39.0339160891174	40.0	38.0	41.0	36.0	41.0
18-19	38.95376354493172	40.0	38.5	41.0	35.0	41.0
20-21	39.013507890028194	40.0	38.5	41.0	35.0	41.0
22-23	38.94314564070067	40.0	38.0	41.0	35.0	41.0
24-25	38.86499823117	40.0	38.0	41.0	35.0	41.0
26-27	38.78301198938382	40.0	38.0	41.0	35.0	41.0
28-29	38.56234126011053	40.0	38.0	41.0	34.5	41.0
30-31	38.49000208853114	40.0	38.0	41.0	34.0	41.0
32-33	38.42938454394012	40.0	38.0	41.0	34.0	41.0
34-35	38.24730453419481	40.0	38.0	41.0	34.0	41.0
36-37	38.368674167896685	40.0	38.0	41.0	34.0	41.0
38-39	38.48449408986709	40.0	38.0	41.0	34.0	41.0
40-41	38.490143695367316	40.0	38.0	41.0	34.0	41.0
42-43	38.36512754173416	40.0	38.0	41.0	34.0	41.0
44-45	38.3081325030071	40.0	38.0	41.0	33.0	41.0
46-47	38.32539001132735	40.0	38.0	41.0	33.5	41.0
48-49	38.204818687230315	40.0	38.0	41.0	33.0	41.0
50-51	38.13506109969957	40.0	37.5	41.0	33.0	41.0
52-53	38.071331839811094	40.0	37.0	41.0	33.0	41.0
54-55	37.96789518414875	40.0	37.0	41.0	33.0	41.0
56-57	37.81294586161246	40.0	37.0	41.0	33.0	41.0
58-59	37.66693249947477	39.5	36.0	41.0	33.0	41.0
60-61	37.49033704316906	39.0	36.0	41.0	32.5	41.0
62-63	37.2943207876629	39.0	35.5	41.0	32.0	41.0
64-65	37.07670244592852	39.0	35.0	40.0	32.0	41.0
66-67	36.81475657081715	38.0	35.0	40.0	31.5	41.0
68-69	36.51982330504592	37.5	35.0	40.0	31.0	41.0
70-71	36.23657204449145	37.0	35.0	40.0	31.0	41.0
72-73	35.91930924065285	36.5	35.0	39.0	31.0	41.0
74-75	35.598104976191976	36.0	34.0	39.0	31.0	40.0
76-77	34.57515345302804	35.0	33.5	37.0	29.5	39.0
78-79	34.883207607251485	35.0	34.0	37.0	30.0	39.0
80-81	34.72423168648285	35.0	34.0	37.0	30.0	39.0
82-83	34.4527619052947	35.0	34.0	36.5	30.0	39.0
84-85	34.173354514489475	35.0	34.0	36.0	30.0	37.5
86-87	33.91559601762451	35.0	34.0	36.0	30.0	37.0
88-89	33.661311809123816	35.0	33.5	35.0	30.0	37.0
90-91	33.530598813188185	35.0	33.0	35.0	30.0	36.0
92-93	33.44935708412089	35.0	33.0	35.0	30.0	36.0
94-95	33.356403345987616	35.0	33.0	35.0	30.0	36.0
96-97	33.33981031459609	35.0	33.0	35.0	30.0	35.5
98-99	33.373123316088495	34.5	33.0	35.0	30.0	35.0
100-101	32.17555143581309	33.0	31.0	34.5	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	9.0
20	50.0
21	350.0
22	1507.0
23	5166.0
24	12642.0
25	25896.0
26	46118.0
27	72519.0
28	102928.0
29	138842.0
30	178400.0
31	224260.0
32	282064.0
33	364872.0
34	501567.0
35	715222.0
36	1173488.0
37	2226040.0
38	3026206.0
39	1457960.0
40	65208.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.02222813067608	22.106281368523447	15.654792682940608	24.21669781785986
2	13.440349576267197	22.157254336468288	41.810779379880984	22.59161670738353
3	16.480088049817066	27.531017977151485	31.823832577302504	24.165061395728944
4	11.600323764297556	19.565445194268463	39.60097377846248	29.233257262971495
5	12.363383868697495	38.46991062749058	36.40204212771066	12.764663376101268
6	28.34749294153668	39.517348736858004	19.23317803521455	12.90198028639077
7	25.09508961180239	33.43436241706787	24.00900891570444	17.461539055425302
8	23.682613927161185	37.391994923389504	21.76238977296479	17.163001376484523
9	24.92580459714923	17.01820628365061	21.84343541332785	36.21255370587231
10-11	23.27091272074913	27.365541586723175	29.70211898171265	19.661426710815043
12-13	24.330200449812605	24.996022148676172	29.62541256673375	21.048364834777473
14-15	21.518700004034347	26.386322307511573	27.221926668166486	24.873051020287594
16-17	20.964440951018766	29.607473077128944	27.872300551523242	21.555785420329048
18-19	21.04956773009897	28.614393386581916	29.67931797148856	20.656720911830554
20-21	21.77266568810311	27.7434396540442	29.591357130819006	20.89253752703368
22-23	21.414057485063097	27.887372470971073	29.461436178351892	21.237133865613945
24-25	21.353653290750124	27.986618086794056	29.52850127300352	21.1312273494523
26-27	21.371953039904128	28.158126014714767	29.439901246171413	21.03001969920969
28-29	21.169157336787563	27.9720806319382	29.48358557227032	21.37517645900391
30-31	21.37195185706206	28.009782860577392	29.543097792932983	21.07516748942756
32-33	21.041990786731564	27.969069402986847	29.687085539246482	21.301854271035108
34-35	21.183049183008144	28.086129604240618	29.493735468708504	21.237085744042737
36-37	21.274933137076285	28.148271094725857	29.455174154303865	21.121621613893993
38-39	21.20702711898025	28.121230835333765	29.421254269672826	21.25048777601316
40-41	21.324692606443264	28.016335841493046	29.428563529015015	21.23040802304867
42-43	21.462975251737202	28.092823227853852	29.16778395683593	21.276417563573023
44-45	21.39961532814651	28.373344717941144	28.88778521223006	21.339254741682286
46-47	21.6019206586021	28.16449617040307	28.513127251360043	21.720455919634787
48-49	21.54590020602945	28.293667353231406	28.49500453869083	21.665427902048318
50-51	21.44888626096141	28.414814674251947	28.482371207048406	21.653927857738235
52-53	21.489660184266206	28.4852577470014	28.522061739944633	21.503020328787763
54-55	21.509119055408895	28.47473257990029	28.346027856236876	21.670120508453934
56-57	21.72593013405836	28.607676555277333	28.10940122504293	21.556992085621378
58-59	21.423129778669452	28.84511662781505	28.40608190799674	21.325671685518763
60-61	21.705474402069633	28.9410746118566	28.012984980449506	21.34046600562426
62-63	21.52556570476006	29.073049720189964	27.959590016166043	21.441794558883938
64-65	21.484786427570885	28.92030215480108	28.060587349458505	21.534324068169532
66-67	21.59185032630849	28.79888655694534	27.997152803818544	21.612110312927626
68-69	21.56876613072915	28.65575519259005	28.12095783553875	21.65452084114205
70-71	21.599232570539616	28.452186273951085	28.16845417923513	21.78012697627417
72-73	21.653718042238296	28.508872151375865	28.159224362523627	21.678185443862212
74-75	21.548876811146755	28.616370580756957	28.122953187977394	21.711799420118894
76-77	21.61647740407806	28.57101205779849	28.049988832176947	21.762521705946504
78-79	21.578101514659576	28.511602584766322	28.08964654648955	21.82064935408456
80-81	21.60895588008533	28.578686460099934	28.081902085360532	21.7304555744542
82-83	21.617148263147566	28.518177826849016	28.217260113514197	21.647413796489225
84-85	21.747862905677668	28.525313106367967	28.07687699764662	21.649946990307743
86-87	21.59037521852761	28.55833628359567	28.141572375063756	21.70971612281297
88-89	21.675231733334353	28.47492596125677	28.06345215045991	21.78639015494896
90-91	21.64598313047688	28.577932112916756	28.159076499612446	21.61700825699392
92-93	21.690005281433297	28.5133731075923	28.15329271187621	21.64332889909819
94-95	21.511885666761664	28.59683925031827	28.158232647104768	21.733042435815296
96-97	21.45760740262299	28.64153581035731	28.21017228462841	21.690684502391292
98-99	21.69168976944917	29.219153364919244	28.25716337032246	20.831993495309128
100-101	22.103859568216162	31.924793260537037	25.551582675131275	20.419764496115526
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3441.0
1	2919.5
2	3500.0
3	4822.5
4	6308.5
5	9211.5
6	11463.5
7	13486.0
8	15163.5
9	15153.5
10	13827.0
11	12666.0
12	14127.5
13	19529.0
14	32189.5
15	49240.0
16	62802.5
17	70002.5
18	71170.5
19	68447.0
20	63857.0
21	59179.5
22	57172.0
23	61443.5
24	72304.0
25	89161.5
26	110513.5
27	133575.0
28	160147.5
29	189825.0
30	217584.0
31	244758.5
32	273165.5
33	300499.0
34	325517.0
35	347997.5
36	370008.5
37	389860.5
38	402651.5
39	408839.0
40	415214.0
41	423233.0
42	427595.5
43	429178.5
44	431268.0
45	431704.0
46	430808.5
47	430941.5
48	434906.5
49	425156.5
50	403513.0
51	384831.5
52	362352.0
53	338353.5
54	311762.0
55	281615.0
56	253893.0
57	226997.5
58	198196.0
59	168701.0
60	138857.0
61	111468.5
62	87247.5
63	66625.0
64	49420.5
65	36750.0
66	27994.0
67	21496.0
68	16455.0
69	11883.0
70	8448.0
71	6181.0
72	4361.0
73	2990.5
74	2092.0
75	1409.5
76	895.5
77	576.0
78	384.0
79	269.5
80	199.0
81	131.5
82	91.5
83	63.5
84	43.0
85	30.0
86	21.5
87	17.0
88	13.0
89	10.5
90	7.5
91	3.5
92	3.0
93	2.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	5.272416337109262E-4
4	8.473526256068456E-5
5	3.860161961097853E-4
6	2.259607001618255E-4
7	0.00265503822690145
8	5.272416337109262E-4
9	3.577711085895571E-4
10-11	1.0827283549420806E-4
12-13	3.766011669363759E-5
14-15	2.0242312722830203E-4
16-17	8.991352860605974E-4
18-19	4.707514586704699E-6
20-21	0.0
22-23	0.0025847186256001175
24-25	0.007101302147126737
26-27	0.03563353183182876
28-29	0.27974856993304137
30-31	0.24396540535438271
32-33	0.25138704168359366
34-35	0.131185541298327
36-37	0.023316289033940493
38-39	0.014262762548535209
40-41	0.007309657248965382
42-43	0.004174930664073511
44-45	0.005133990533156827
46-47	9.263190473635846E-4
48-49	0.0016657763350609552
50-51	0.0023164298718519705
52-53	0.0012108438289242507
54-55	8.20754837965041E-5
56-57	0.001403266472950304
58-59	0.0025573270085947107
60-61	0.011126390085530558
62-63	0.0064738975174017345
64-65	0.0013212020026186764
66-67	0.0017614698271398216
68-69	0.0023501446276797166
70-71	0.003897665265049881
72-73	0.003532240141627619
74-75	0.0035624535380086196
76-77	0.0014894042687706485
78-79	1.0464159295199339E-4
80-81	3.999005894181835E-4
82-83	1.7852257926804197E-5
84-85	0.0
86-87	0.0
88-89	0.0
90-91	4.5693586072695114E-4
92-93	0.007713144227029221
94-95	0.001000991562868147
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	183.0
20-21	713.0
22-23	1628.0
24-25	3991.0
26-27	5848.0
28-29	9870.0
30-31	21535.0
32-33	28283.0
34-35	41115.0
36-37	58164.0
38-39	69397.0
40-41	72993.0
42-43	86152.0
44-45	101645.0
46-47	101318.0
48-49	90323.0
50-51	80877.0
52-53	79033.0
54-55	87983.0
56-57	95837.0
58-59	95953.0
60-61	97078.0
62-63	95716.0
64-65	98518.0
66-67	108832.0
68-69	97834.0
70-71	92905.0
72-73	89262.0
74-75	90772.0
76-77	92287.0
78-79	98231.0
80-81	100223.0
82-83	97384.0
84-85	98405.0
86-87	103687.0
88-89	110444.0
90-91	116921.0
92-93	124118.0
94-95	140291.0
96-97	212585.0
98-99	881804.0
100-101	6541178.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.51427788092938
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.24576401910448	72.81535494087308
2	7.6966129501127	12.701609194151178
3	2.1730181567998503	5.379150720914636
4	0.8710046236272463	2.8748127019821164
5	0.3949888109116771	1.6296108251711998
6	0.2107092798824056	1.0431914443384405
7	0.13010202617398964	0.7514692318414771
8	0.07337550545671355	0.4843621477527131
9	0.05063019368775691	0.3759942484005166
>10	0.15173820128496154	1.789840532362387
>50	0.0017345238107403565	0.09477742829149446
>100	3.021105121725996E-4	0.04939585526569418
>500	1.9598635228666E-5	0.010430728655076013
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	9.415029173409396E-6	0.0
3	0.0	0.0	0.0	1.8830058346818792E-5	0.0
4	0.0	9.415029173409396E-6	0.0	2.824508752022819E-5	0.0
5	0.0	9.415029173409396E-6	0.0	3.7660116693637584E-5	0.0
6	1.6947052512136915E-4	9.415029173409396E-6	0.0	3.7660116693637584E-5	0.0
7	1.7888555429477853E-4	9.415029173409396E-6	0.0	3.7660116693637584E-5	0.0
8	1.7888555429477853E-4	3.7660116693637584E-5	0.0	3.7660116693637584E-5	0.0
9	1.7888555429477853E-4	8.473526256068457E-5	0.0	3.7660116693637584E-5	0.0
10-11	2.353757293352349E-4	8.473526256068457E-5	0.0	4.236763128034229E-5	0.0
12-13	2.4949827309534903E-4	8.473526256068457E-5	0.0	5.178266045375168E-5	0.0
14-15	2.6832833144216784E-4	1.1298035008091276E-4	0.0	5.649017504045638E-5	0.0
16-17	4.236763128034228E-4	1.1298035008091276E-4	0.0	6.590520421386577E-5	0.0
18-19	4.3779885656353694E-4	1.2710289384102686E-4	0.0	8.473526256068457E-5	0.0
20-21	4.425063711502416E-4	1.4122543760114096E-4	0.0	8.473526256068457E-5	0.0
22-23	4.6133642949706044E-4	1.5534798136125502E-4	0.0	1.1298035008091276E-4	0.0
24-25	5.319491482976309E-4	1.7888555429477853E-4	0.0	1.6005549594795974E-4	0.0
26-27	5.554867212311544E-4	1.8830058346818793E-4	0.0	1.8359306888148322E-4	0.0
28-29	5.743167795779732E-4	1.8830058346818793E-4	0.0	3.295260210693289E-4	0.0
30-31	5.978543525114966E-4	2.1654567098841612E-4	0.0	9.367954027542349E-4	0.0
32-33	6.355144692051343E-4	2.1654567098841612E-4	0.0	0.0025514729059939465	0.0
34-35	6.778821004854766E-4	2.636208168554631E-4	0.0	0.004533336546996625	0.0
36-37	6.967121588322953E-4	3.106959627225101E-4	0.0	0.007668541261741954	0.0
38-39	7.01419673419E-4	3.106959627225101E-4	0.0	0.012602016548608477	0.0
40-41	7.532023338727517E-4	3.389410502427383E-4	0.0	0.017596689525102162	0.0
42-43	7.532023338727517E-4	3.671861377629665E-4	0.0	0.022351279257673908	0.0
44-45	7.720323922195706E-4	4.425063711502417E-4	0.0	0.02767077074065022	0.0
46-47	7.908624505663894E-4	4.895815170172887E-4	0.0	0.033818784790886555	0.0
48-49	7.908624505663894E-4	4.895815170172887E-4	0.0	0.03967022542216049	0.0
50-51	8.144000234999128E-4	5.366566628843356E-4	0.0	0.0454840059367408	0.0
52-53	8.285225672600269E-4	5.366566628843356E-4	0.0	0.0511000708386795	0.0
54-55	8.285225672600269E-4	5.366566628843356E-4	0.0	0.05682911609069912	0.0
56-57	8.285225672600269E-4	5.46071692057745E-4	0.0	0.06276058446994703	0.0
58-59	8.285225672600269E-4	5.554867212311544E-4	0.0	0.06800004820494937	0.0
60-61	8.850127423004834E-4	5.601942358178591E-4	0.0	0.07318302176491123	0.0
62-63	9.132578298207115E-4	5.743167795779732E-4	0.0	0.07813532711012458	0.0
64-65	9.132578298207115E-4	7.061271880057047E-4	0.0	0.08330417812632634	0.0
66-67	9.462104319276443E-4	7.249572463525235E-4	0.0	0.08853422683215526	0.0
68-69	9.885780632079867E-4	7.626173630461612E-4	0.0	0.09355714489616918	0.0
70-71	0.0010027006069681008	7.8144742139298E-4	0.0	0.09846708261010217	0.0
72-73	0.0011156809570490136	7.861549359796847E-4	0.0	0.10240727231917401	0.0
74-75	0.0011203884716357183	8.285225672600269E-4	0.0	0.10615445393019095	0.0
76-77	0.0011345110153958323	8.708901985403692E-4	0.0	0.1094120540241906	0.0
78-79	0.001139218529982537	8.850127423004833E-4	0.0	0.1124295708742683	0.0
80-81	0.001139218529982537	8.850127423004833E-4	0.0	0.11366293969598494	0.0
82-83	0.0011627561029160605	8.850127423004833E-4	0.0	0.11414310618382882	0.0
84-85	0.0011815861612628792	8.850127423004833E-4	0.0	0.11438318942775076	0.0
86-87	0.001186293675849584	8.944277714738927E-4	0.0	0.1145149998361785	0.0
88-89	0.0012051237341964027	9.415029173409396E-4	0.0	0.11456207498204554	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14185	0.0	48.014458	1
GTATCAA	25330	0.0	35.90718	1
CTTATAC	9670	0.0	34.11863	1
ATCAACG	31360	0.0	28.461363	3
TCAACGC	31540	0.0	28.298935	4
CAACGCA	32255	0.0	27.69796	5
AACGCAG	32945	0.0	27.23385	6
TATCAAC	33225	0.0	27.157574	2
TATACAC	14005	0.0	26.620148	3
TTATACA	14930	0.0	23.747818	2
ACGCAGA	37910	0.0	23.599771	7
CGCAGAG	38415	0.0	23.27793	8
ACATCTC	16535	0.0	20.33798	8
GCAGAGT	44655	0.0	19.844456	9
ACACATC	17005	0.0	19.776417	6
TACACAT	19110	0.0	19.464466	5
GTACATG	30345	0.0	19.254326	1
TACATGG	30225	0.0	18.867168	2
GAGTACT	26870	0.0	18.709959	12-13
AGAGTAC	40975	0.0	18.52311	10-11
>>END_MODULE