FastQCFastQC Report
Fri 27 May 2016
SRR2139020_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139020_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10837265
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA143200.048.5422631
GTATCAA260250.036.8812141
ATCAACG319500.029.4700343
TCAACGC322000.029.3466534
CAACGCA328600.028.7572215
TATCAAC336150.028.3010562
AACGCAG339250.027.9045496
CTTATAC85950.026.6948551
ACGCAGA390150.024.242277
CGCAGAG390750.024.171648
TATACAC134600.020.6765423
GCAGAGT455850.020.6450259
GTACATG315400.019.4394661
TACATGG315850.019.075552
GAGTACT278950.019.04701812-13
AGAGTAC416650.018.61050610-11
ACATGGG315650.018.2799783
CAGAGTA456400.017.7250710-11
TGGTATC70100.017.6989122
TTATACA140950.017.6348782
Produced by FastQC (version 0.11.5)