##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139020_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10837265
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.49319371631127	34.0	31.0	34.0	31.0	34.0
2	32.64137538391836	34.0	31.0	34.0	31.0	34.0
3	32.63694225434185	34.0	31.0	34.0	31.0	34.0
4	36.196160285828576	37.0	35.0	37.0	35.0	37.0
5	36.134627786623284	37.0	35.0	37.0	35.0	37.0
6	36.160084117164246	37.0	36.0	37.0	35.0	37.0
7	36.10989036440467	37.0	36.0	37.0	35.0	37.0
8	36.097611712918344	37.0	36.0	37.0	35.0	37.0
9	37.80401614244923	39.0	38.0	39.0	35.0	39.0
10-11	37.74639634631062	39.0	38.0	39.0	35.0	39.0
12-13	37.71835310846417	39.0	38.0	39.0	35.0	39.0
14-15	39.0637360994679	40.0	38.0	41.0	36.0	41.0
16-17	39.03650616645436	40.0	38.0	41.0	36.0	41.0
18-19	38.96224651699483	40.0	38.5	41.0	35.0	41.0
20-21	39.02212334121921	40.0	39.0	41.0	35.0	41.0
22-23	38.951599173971644	40.0	38.0	41.0	35.0	41.0
24-25	38.87550530681459	40.0	38.0	41.0	35.0	41.0
26-27	38.79779258393138	40.0	38.0	41.0	35.0	41.0
28-29	38.584288896400395	40.0	38.0	41.0	34.5	41.0
30-31	38.510765258027334	40.0	38.0	41.0	34.0	41.0
32-33	38.44908475647328	40.0	38.0	41.0	34.0	41.0
34-35	38.265675562417115	40.0	38.0	41.0	34.0	41.0
36-37	38.38833558222244	40.0	38.0	41.0	34.0	41.0
38-39	38.50521850394615	40.0	38.0	41.0	34.0	41.0
40-41	38.5072045501755	40.0	38.0	41.0	34.0	41.0
42-43	38.38432071568961	40.0	38.0	41.0	34.0	41.0
44-45	38.327203604842566	40.0	38.0	41.0	34.0	41.0
46-47	38.34453099556476	40.0	38.0	41.0	34.0	41.0
48-49	38.22474251297449	40.0	38.0	41.0	33.0	41.0
50-51	38.16083947959446	40.0	37.5	41.0	33.0	41.0
52-53	38.093214031635625	40.0	37.0	41.0	33.0	41.0
54-55	37.990647046446455	40.0	37.0	41.0	33.0	41.0
56-57	37.84063994625302	40.0	37.0	41.0	33.0	41.0
58-59	37.69415266884287	39.5	36.0	41.0	33.0	41.0
60-61	37.51731710046756	39.0	36.0	41.0	32.5	41.0
62-63	37.31379353713233	39.0	35.5	41.0	32.0	41.0
64-65	37.08886846969135	39.0	35.0	40.0	32.0	41.0
66-67	36.82346405509021	38.0	35.0	40.0	32.0	41.0
68-69	36.525589995821804	37.5	35.0	40.0	31.0	41.0
70-71	36.23873961128252	37.0	35.0	39.5	31.0	41.0
72-73	35.915133244493646	36.5	35.0	39.0	31.0	41.0
74-75	35.59230635284691	36.0	34.0	39.0	31.0	40.0
76-77	34.56821534113948	35.0	33.5	37.0	29.5	39.0
78-79	34.87923997698195	35.0	34.0	37.0	30.0	39.0
80-81	34.72263018213884	35.0	34.0	37.0	30.0	39.0
82-83	34.452334923875185	35.0	34.0	36.5	30.0	39.0
84-85	34.17406597844234	35.0	34.0	36.0	30.0	37.5
86-87	33.91624015580352	35.0	34.0	36.0	30.0	37.0
88-89	33.66533510008634	35.0	33.5	35.0	30.0	37.0
90-91	33.53285741997941	35.0	33.0	35.0	30.0	36.0
92-93	33.45206964561561	35.0	33.0	35.0	30.0	36.0
94-95	33.35834975362811	35.0	33.0	35.0	30.0	36.0
96-97	33.34407364684465	35.0	33.0	35.0	30.0	35.5
98-99	33.3750561246426	34.5	33.0	35.0	30.0	35.0
100-101	32.1557918899347	33.0	31.0	34.5	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	14.0
20	61.0
21	363.0
22	1596.0
23	5345.0
24	13147.0
25	26963.0
26	46502.0
27	73929.0
28	104673.0
29	140551.0
30	179874.0
31	226949.0
32	283779.0
33	366207.0
34	504735.0
35	719789.0
36	1191377.0
37	2294536.0
38	3107841.0
39	1483370.0
40	65663.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.356157052185864	21.8761764962079	15.477033686731264	24.29063276487497
2	13.525341820592358	22.189355173039985	41.49994869553792	22.785354310829746
3	16.589583438680307	27.420578222943853	31.7754930146189	24.214345323756937
4	11.624772866607046	19.426433510217173	39.598979223869094	29.34981439930669
5	12.3964690190238	38.503305507918775	36.28422062310289	12.816004849954531
6	28.45652718131355	39.414268001557225	19.21019949446552	12.919005322663708
7	25.126446713548134	33.481222067853615	24.0135532273948	17.37877799120345
8	23.838767515987335	37.196884685126776	21.711967080707687	17.252380718178202
9	25.1302501692777	16.7804258323766	21.797135834257155	36.29218816408854
10-11	23.270265713916356	27.349360636183125	29.70515509598047	19.675218553920057
12-13	24.29355087165459	24.993556952525743	29.63871043823519	21.074181737584478
14-15	21.500963251391912	26.37457330057965	27.31026697353407	24.81419647449437
16-17	20.906491142213866	29.54325535734199	27.98246828585772	21.567785214586422
18-19	21.015287716095845	28.59882610581657	29.756240113393943	20.62964606469364
20-21	21.78364660112147	27.741798676589585	29.665495094076306	20.80905962821264
22-23	21.453849199281276	27.803124616436676	29.514792970397448	21.2282332138846
24-25	21.293226489439647	27.902645136352884	29.60937364786514	21.194754726342328
26-27	21.307746865167207	28.08031847867359	29.500156691995077	21.11177796416413
28-29	21.182372072361275	27.991211698835315	29.500073884357782	21.326342344445635
30-31	21.386901507908192	27.966638208104534	29.547632178400324	21.098828105586954
32-33	21.13685371937814	27.944478368259684	29.608293994174517	21.310373918187654
34-35	21.26814452348959	28.078736640042358	29.46222550356974	21.190893332898316
36-37	21.30855767757384	28.104473058755104	29.44620579837111	21.140763465299948
38-39	21.217309234166287	28.10102492284612	29.45073010052015	21.230935742467445
40-41	21.312369477095437	28.031086576815945	29.40051806234945	21.25602588373917
42-43	21.40676568383487	28.103105475265693	29.14630364825845	21.34382519264099
44-45	21.42458095191431	28.296148129787458	28.89134676631398	21.387924151984254
46-47	21.55316661311021	28.22746705598699	28.54220140222476	21.67716492867804
48-49	21.545646708525663	28.303401275666435	28.504528511467818	21.64642350434008
50-51	21.4487027452426	28.44487242022612	28.456204099160693	21.65022073537059
52-53	21.51761814746686	28.420578917230117	28.470864508489495	21.590938426813533
54-55	21.568617795718332	28.382351404216386	28.342400195841815	21.706630604223463
56-57	21.644480060705636	28.517048180481613	28.201298970323702	21.637172788489053
58-59	21.51322521768287	28.65778618073895	28.368288148843273	21.460700452734905
60-61	21.71012127628958	28.69325316326563	28.15063262727273	21.445992933172057
62-63	21.57914607781453	28.79968079665937	28.112039411985933	21.509133713540166
64-65	21.55660485533555	28.690216074741915	28.151815103221416	21.601363966701122
66-67	21.642476177899045	28.623554447558707	28.059421473654634	21.67454790088761
68-69	21.619262971047917	28.52029799883561	28.141692920808335	21.718746109308135
70-71	21.620513942638247	28.35068765549189	28.223654311513872	21.805144090355988
72-73	21.674385588878497	28.39494100329581	28.18390537269802	21.746768035127676
74-75	21.58676273356279	28.459734528339297	28.157496762771395	21.796005975326523
76-77	21.681666137480846	28.41401744362147	28.083759385551442	21.82055703334624
78-79	21.635001986878134	28.34000638962268	28.111701612012308	21.91329001148688
80-81	21.646733795082824	28.359393230057194	28.15367476530905	21.840198209550934
82-83	21.64663352577826	28.33020775539642	28.240484095535145	21.782674623290177
84-85	21.745211026998984	28.338560313803857	28.13367069321823	21.78255796597893
86-87	21.661630437538875	28.29833728365819	28.20367268339178	21.83635959541116
88-89	21.691395651031037	28.228521297459725	28.19510573943956	21.884977312069687
90-91	21.710690309792234	28.244808426153455	28.247980295391212	21.796520968663096
92-93	21.72798594949947	28.194326200468932	28.26040294222866	21.81728490780294
94-95	21.62866489154747	28.19946472587644	28.289469430538162	21.882400952037933
96-97	21.555902414932092	28.268956585007466	28.367825513859522	21.807315486200917
98-99	21.76190934998658	28.811532953622145	28.437182002195588	20.989375694195683
100-101	22.157299078030253	31.560661846437547	25.64763638878354	20.634402686748658
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2492.0
1	2209.5
2	2978.5
3	4247.5
4	5664.0
5	8426.0
6	10667.0
7	12861.5
8	14706.5
9	14889.0
10	13626.5
11	12628.0
12	14504.5
13	20337.0
14	33685.0
15	51332.0
16	65056.0
17	72262.5
18	73207.5
19	69651.0
20	63974.5
21	58964.5
22	57972.5
23	63118.0
24	74160.0
25	91580.5
26	114597.5
27	139472.0
28	167288.5
29	196925.0
30	225041.5
31	253061.0
32	280225.5
33	304670.5
34	327339.5
35	349003.0
36	370364.5
37	389150.0
38	402708.0
39	412000.5
40	421204.5
41	430226.5
42	434415.5
43	436071.0
44	437027.5
45	437663.5
46	439025.0
47	437694.0
48	436987.5
49	428871.5
50	412028.5
51	393573.5
52	370267.0
53	345566.0
54	319905.5
55	292197.5
56	264361.5
57	235313.0
58	204940.5
59	175130.5
60	145007.0
61	116942.5
62	91986.0
63	70204.5
64	51947.0
65	38107.5
66	28444.5
67	21613.0
68	16280.5
69	11874.5
70	8599.0
71	6174.0
72	4393.5
73	3113.0
74	2153.0
75	1430.5
76	934.0
77	605.5
78	402.5
79	291.5
80	214.0
81	142.5
82	96.5
83	62.5
84	38.5
85	25.5
86	19.5
87	15.0
88	9.0
89	4.0
90	1.5
91	1.0
92	1.0
93	1.5
94	1.5
95	1.0
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	9.227420386970328E-6
2	9.227420386970328E-6
3	5.6287264360519E-4
4	1.9377582812637691E-4
5	4.6137101934851647E-4
6	2.0300324851334726E-4
7	0.002666724491834425
8	8.673775163752109E-4
9	3.967790766397242E-4
10-11	7.381936309576262E-5
12-13	1.8454840773940656E-5
14-15	1.0150162425667363E-4
16-17	9.642654304383994E-4
18-19	1.8454840773940656E-5
20-21	0.0
22-23	0.0027315884020447786
24-25	0.006839387506543452
26-27	0.03535287599200872
28-29	0.28096485618133077
30-31	0.24607198176597828
32-33	0.2529809102734098
34-35	0.13393714742786622
36-37	0.02536415817600616
38-39	0.016652369509647574
40-41	0.010052074186670467
42-43	0.00669352550892574
44-45	0.007344270837110941
46-47	0.0016352971861484991
48-49	0.0022689456224394113
50-51	0.0032332802824878424
52-53	0.0020083930896725587
54-55	2.964550756249361E-4
56-57	0.002221036880418817
58-59	0.0037168887797827876
60-61	0.01380352231768481
62-63	0.008821896762092446
64-65	0.002430323777117484
66-67	0.0018065998128810245
68-69	0.0024684230484744393
70-71	0.004418592740867787
72-73	0.0037659918383689157
74-75	0.003977532891554596
76-77	0.0017317486483786138
78-79	9.665730606990825E-5
80-81	4.142197021300098E-4
82-83	2.9112145924980793E-5
84-85	0.0
86-87	1.789951783472158E-5
88-89	0.0
90-91	3.9264764824611356E-4
92-93	0.00810156621970949
94-95	0.0013818405863060866
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	161.0
20-21	628.0
22-23	1474.0
24-25	3157.0
26-27	5457.0
28-29	9680.0
30-31	21096.0
32-33	28942.0
34-35	41364.0
36-37	57894.0
38-39	69615.0
40-41	74633.0
42-43	88366.0
44-45	104201.0
46-47	103998.0
48-49	91975.0
50-51	82034.0
52-53	80351.0
54-55	88234.0
56-57	94222.0
58-59	94462.0
60-61	94324.0
62-63	92998.0
64-65	97809.0
66-67	107558.0
68-69	100816.0
70-71	97219.0
72-73	93282.0
74-75	94866.0
76-77	97076.0
78-79	102402.0
80-81	103939.0
82-83	102004.0
84-85	103929.0
86-87	109891.0
88-89	116850.0
90-91	125454.0
92-93	131628.0
94-95	145247.0
96-97	216172.0
98-99	906347.0
100-101	6655510.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.75714576498955
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.87280237183711	74.43732262801069
2	7.527693501956286	12.609962438350344
3	1.9797922045767309	4.974652327893698
4	0.7569850564534814	2.536116308611723
5	0.3349904209199911	1.4028920757435452
6	0.18535039776996348	0.9314652170170576
7	0.10578325852510838	0.6202072662648153
8	0.06619737741751826	0.4435602711695284
9	0.04163103657138452	0.3138207118611358
>10	0.1265949861585117	1.566748385882764
>50	0.001770072367046599	0.09421199368278788
>100	3.909528922755612E-4	0.05792067049732974
>500	1.836255458590617E-5	0.011119705014531186
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	9.22742038697033E-6	0.0	0.0	0.0
2	0.0	1.845484077394066E-5	0.0	0.0	9.22742038697033E-6
3	0.0	1.845484077394066E-5	0.0	2.7682261160910985E-5	9.22742038697033E-6
4	0.0	5.536452232182197E-5	0.0	3.690968154788132E-5	2.7682261160910985E-5
5	0.0	5.536452232182197E-5	0.0	3.690968154788132E-5	2.7682261160910985E-5
6	5.536452232182197E-5	5.536452232182197E-5	0.0	3.690968154788132E-5	3.690968154788132E-5
7	5.536452232182197E-5	5.536452232182197E-5	0.0	3.690968154788132E-5	3.690968154788132E-5
8	5.536452232182197E-5	5.536452232182197E-5	0.0	5.536452232182197E-5	3.690968154788132E-5
9	5.536452232182197E-5	1.6609356696546592E-4	0.0	9.227420386970328E-5	6.45919427087923E-5
10-11	5.536452232182197E-5	1.6609356696546592E-4	0.0	1.4763872619152527E-4	6.45919427087923E-5
12-13	5.536452232182197E-5	1.6609356696546592E-4	0.0	1.6147985677198074E-4	6.45919427087923E-5
14-15	5.536452232182197E-5	1.7532098735243624E-4	0.0	2.0761695870683239E-4	8.766049367621812E-5
16-17	1.1072904464364394E-4	1.7532098735243624E-4	0.0	2.3529921986774338E-4	9.227420386970328E-5
18-19	1.1072904464364394E-4	1.8454840773940656E-4	0.0	3.229597135439615E-4	1.0150162425667362E-4
20-21	1.1072904464364394E-4	1.9377582812637691E-4	0.0	3.6909681547881313E-4	1.0611533445015878E-4
22-23	1.1072904464364394E-4	2.0761695870683239E-4	0.0	4.4291617857457577E-4	1.1072904464364394E-4
24-25	1.1072904464364394E-4	2.2607179948077303E-4	0.0	4.890532805094275E-4	1.3379759561106978E-4
26-27	1.1072904464364394E-4	2.306855096742582E-4	0.0	5.121218314768532E-4	1.4763872619152527E-4
28-29	1.1072904464364394E-4	2.306855096742582E-4	0.0	6.874428188292894E-4	1.5225243638501045E-4
30-31	1.1072904464364394E-4	2.583677708351692E-4	0.0	0.0014671598415282824	1.568661465784956E-4
32-33	1.1072904464364394E-4	2.583677708351692E-4	0.0	0.0031557777723438523	1.568661465784956E-4
34-35	1.291838854175846E-4	2.860500319960802E-4	0.0	0.005541065942375682	1.707072771589511E-4
36-37	1.291838854175846E-4	3.229597135439615E-4	0.0	0.008867550991878486	1.8454840773940656E-4
38-39	1.291838854175846E-4	3.229597135439615E-4	0.0	0.0137765386377467	1.9377582812637691E-4
40-41	1.291838854175846E-4	3.506419747048725E-4	0.0	0.019040781968513273	1.9377582812637691E-4
42-43	1.3841130580455492E-4	3.737105256722983E-4	0.0	0.023760607496448594	1.983895383198621E-4
44-45	1.430250159980401E-4	4.244613378006351E-4	0.0	0.02927399117766337	2.0761695870683239E-4
46-47	1.4763872619152527E-4	4.613710193485164E-4	0.0	0.035410225734998635	2.2145808928728788E-4
48-49	1.4763872619152527E-4	4.613710193485164E-4	0.0	0.04163412078601012	2.306855096742582E-4
50-51	1.5225243638501045E-4	5.028944110898829E-4	0.0	0.047479691601155824	2.306855096742582E-4
52-53	1.6147985677198074E-4	5.07508121283368E-4	0.0	0.05351442453423442	2.4452664025471373E-4
54-55	1.6609356696546592E-4	5.07508121283368E-4	0.0	0.059516861495958626	2.629814810286544E-4
56-57	1.6609356696546592E-4	5.167355416703384E-4	0.0	0.06539934199265221	2.6759519122213955E-4
58-59	1.6609356696546592E-4	5.213492518638236E-4	0.0	0.07100499987773667	2.6759519122213955E-4
60-61	1.7532098735243624E-4	5.305766722507939E-4	0.0	0.0760708536701834	2.6759519122213955E-4
62-63	1.8454840773940656E-4	5.398040926377643E-4	0.0	0.08115054859321055	3.0450487277002084E-4
64-65	1.8454840773940656E-4	6.366920067009527E-4	0.0	0.08633635885068788	3.1373229315699114E-4
66-67	1.983895383198621E-4	6.505331372814082E-4	0.0	0.09168826267513067	3.229597135439615E-4
68-69	2.0300324851334724E-4	6.782153984423192E-4	0.0	0.09722932861750636	3.229597135439615E-4
70-71	2.1223066890031756E-4	6.920565290227747E-4	0.0	0.10249357194827292	3.414145543179022E-4
72-73	2.583677708351692E-4	7.01283949409745E-4	0.0	0.10643368045350926	4.1523391741366477E-4
74-75	2.583677708351692E-4	7.197387901836857E-4	0.0	0.1097463243724316	4.1523391741366477E-4
76-77	2.7220890141562467E-4	7.520347615380818E-4	0.0	0.11306358200154744	4.1523391741366477E-4
78-79	2.7682261160910985E-4	7.658758921185373E-4	0.0	0.11623781461466523	4.1523391741366477E-4
80-81	2.7682261160910985E-4	7.704896023120225E-4	0.0	0.11751581233826061	4.1984762760714995E-4
82-83	2.81436321802595E-4	7.751033125055077E-4	0.0	0.11805561643089837	4.3368875818760547E-4
84-85	2.860500319960802E-4	7.843307328924779E-4	0.0	0.11843394066676416	4.3368875818760547E-4
86-87	2.9066374218956537E-4	7.935581532794483E-4	0.0	0.11851698745024691	4.3368875818760547E-4
88-89	2.9527745238305055E-4	8.350815450208147E-4	0.0	0.11857235197256873	4.3368875818760547E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14320	0.0	48.542263	1
GTATCAA	26025	0.0	36.881214	1
ATCAACG	31950	0.0	29.470034	3
TCAACGC	32200	0.0	29.346653	4
CAACGCA	32860	0.0	28.757221	5
TATCAAC	33615	0.0	28.301056	2
AACGCAG	33925	0.0	27.904549	6
CTTATAC	8595	0.0	26.694855	1
ACGCAGA	39015	0.0	24.24227	7
CGCAGAG	39075	0.0	24.17164	8
TATACAC	13460	0.0	20.676542	3
GCAGAGT	45585	0.0	20.645025	9
GTACATG	31540	0.0	19.439466	1
TACATGG	31585	0.0	19.07555	2
GAGTACT	27895	0.0	19.047018	12-13
AGAGTAC	41665	0.0	18.610506	10-11
ACATGGG	31565	0.0	18.279978	3
CAGAGTA	45640	0.0	17.72507	10-11
TGGTATC	7010	0.0	17.698912	2
TTATACA	14095	0.0	17.634878	2
>>END_MODULE