FastQCFastQC Report
Fri 27 May 2016
SRR2139019_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139019_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8349889
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA108950.048.9641271
GTATCAA192100.037.500781
ATCAACG244150.029.0346073
TCAACGC245650.028.8399414
CAACGCA250450.028.2531285
TATCAAC254850.028.1000942
AACGCAG257500.027.5790396
ACGCAGA296050.023.8867957
CGCAGAG311000.022.8204658
GTGGTAT55450.021.0114651
GTACATG238350.020.9133171
TACATGG237500.020.4672992
GCAGAGT347200.020.2688189
CTTATAC52050.020.1701721
ACATGGG239600.019.6646083
AGAGTAC317500.018.98622710-11
TGGTATC56300.018.8751932
GAGTACT217400.018.66912712-13
CAGAGTA338450.018.0316510-11
CATGGGG180850.017.2269124
Produced by FastQC (version 0.11.5)