##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139019_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8349889
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.51590242696639	34.0	31.0	34.0	31.0	34.0
2	32.65836432077121	34.0	31.0	34.0	31.0	34.0
3	32.65244328397659	34.0	31.0	34.0	31.0	34.0
4	36.20697987721753	37.0	35.0	37.0	35.0	37.0
5	36.14458072436651	37.0	35.0	37.0	35.0	37.0
6	36.169753035040344	37.0	36.0	37.0	35.0	37.0
7	36.119988900451254	37.0	36.0	37.0	35.0	37.0
8	36.10736909197236	37.0	36.0	37.0	35.0	37.0
9	37.81905352274743	39.0	38.0	39.0	35.0	39.0
10-11	37.76357631820015	39.0	38.0	39.0	35.0	39.0
12-13	37.732013982461325	39.0	38.0	39.0	35.0	39.0
14-15	39.08295319853953	40.0	38.0	41.0	36.0	41.0
16-17	39.0568593786097	40.0	38.0	41.0	36.0	41.0
18-19	38.97702969464624	40.0	38.5	41.0	35.0	41.0
20-21	39.03655842343021	40.0	39.0	41.0	35.0	41.0
22-23	38.963862499503904	40.0	38.0	41.0	35.0	41.0
24-25	38.889555638004126	40.0	38.0	41.0	35.0	41.0
26-27	38.80989148459702	40.0	38.0	41.0	35.0	41.0
28-29	38.594102580632054	40.0	38.0	41.0	34.5	41.0
30-31	38.52234582483479	40.0	38.0	41.0	34.0	41.0
32-33	38.45774413763388	40.0	38.0	41.0	34.0	41.0
34-35	38.27095599011524	40.0	38.0	41.0	34.0	41.0
36-37	38.39245533209022	40.0	38.0	41.0	34.0	41.0
38-39	38.508282020347075	40.0	38.0	41.0	34.0	41.0
40-41	38.50080493218759	40.0	38.0	41.0	34.0	41.0
42-43	38.37482360034147	40.0	38.0	41.0	34.0	41.0
44-45	38.31383466772647	40.0	38.0	41.0	34.0	41.0
46-47	38.329687027564574	40.0	38.0	41.0	34.0	41.0
48-49	38.19947691590882	40.0	38.0	41.0	33.0	41.0
50-51	38.130137716310145	40.0	37.5	41.0	33.0	41.0
52-53	38.0561825611408	40.0	37.0	41.0	33.0	41.0
54-55	37.94792873366818	40.0	37.0	41.0	33.0	41.0
56-57	37.79125539719746	40.0	36.5	41.0	33.0	41.0
58-59	37.62911815641806	39.0	36.0	41.0	33.0	41.0
60-61	37.44315928356872	39.0	36.0	41.0	32.5	41.0
62-63	37.225385471458075	39.0	35.0	40.5	32.0	41.0
64-65	36.98432520399767	38.5	35.0	40.0	32.0	41.0
66-67	36.70640620786163	38.0	35.0	40.0	31.5	41.0
68-69	36.39497301292307	37.0	35.0	40.0	31.0	41.0
70-71	36.099267036420784	37.0	35.0	39.5	31.0	41.0
72-73	35.77447712810134	36.0	34.5	39.0	31.0	40.5
74-75	35.451263971358046	36.0	34.0	39.0	31.0	40.0
76-77	34.43926396066094	35.0	33.5	37.0	29.5	39.0
78-79	34.75053968463007	35.0	34.0	37.0	30.0	39.0
80-81	34.59833017363189	35.0	34.0	37.0	30.0	39.0
82-83	34.34128086014453	35.0	34.0	36.0	30.0	38.5
84-85	34.07716711280257	35.0	34.0	36.0	30.0	37.0
86-87	33.83573130552461	35.0	34.0	35.5	30.0	37.0
88-89	33.60064495304953	35.0	33.0	35.0	30.0	36.5
90-91	33.485325786687085	35.0	33.0	35.0	30.0	36.0
92-93	33.412376348783724	35.0	33.0	35.0	30.0	36.0
94-95	33.32863994194508	35.0	33.0	35.0	29.5	36.0
96-97	33.32187016599346	35.0	33.0	35.0	30.0	35.0
98-99	33.3584659280549	34.5	33.0	35.0	30.0	35.0
100-101	32.145852231301106	33.0	31.0	34.5	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	5.0
20	42.0
21	281.0
22	1163.0
23	4146.0
24	10366.0
25	21073.0
26	36877.0
27	57976.0
28	82126.0
29	109604.0
30	139857.0
31	175234.0
32	220601.0
33	284687.0
34	393286.0
35	569432.0
36	949672.0
37	1817419.0
38	2360921.0
39	1067897.0
40	47224.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	38.990889579490215	21.32776854877951	15.254118946970433	24.42722292475984
2	13.847561326863147	21.977525689263654	41.09910922169145	23.07580376218175
3	16.930332523896276	26.94159502054891	31.37471203027221	24.753360425282604
4	11.973620673466613	19.064364100431717	39.023041311291955	29.938973914809715
5	12.83976447523403	38.06525015673906	35.92383828960109	13.171147078425818
6	29.16907738486248	38.80245699839626	18.887687139297974	13.140778477443282
7	25.67220054071624	33.07872434657865	23.648610615083285	17.60046449762182
8	24.159631685517567	36.91304280654639	21.358242290463473	17.569083217472567
9	25.25174679617306	16.778668571369717	21.495718849350492	36.47386578310673
10-11	23.72894783502069	27.07713826186916	29.268228712412576	19.92568519069757
12-13	24.670390788475245	24.736026399396017	29.275602259575216	21.31798055255352
14-15	21.798789479101877	26.024289101760967	26.995704667009147	25.18121675212801
16-17	21.290777371999862	29.190057181571476	27.570636226197127	21.94852922023154
18-19	21.412857358856634	28.28013551080778	29.34194622382637	20.96506090650922
20-21	22.163125663803303	27.397758123371478	29.31537417547067	21.12374203735455
22-23	21.84423142710121	27.419239308647086	29.14894516648029	21.587584097771416
24-25	21.714134509868245	27.548600178977146	29.23001846203541	21.5072468491192
26-27	21.626960456770586	27.689492686680495	29.170129202597412	21.51341765395151
28-29	21.635591823520155	27.589831673832215	29.125256309502756	21.649320193144874
30-31	21.842246593043868	27.54357812961888	29.156592398730137	21.457582878607116
32-33	21.608887561548638	27.60511967894234	29.15247488781871	21.633517871690316
34-35	21.788998931591983	27.71589348301316	28.981097605732977	21.514009979661886
36-37	21.824159976798235	27.714777060236784	28.946249749957474	21.514813213007507
38-39	21.65111522566801	27.756199709137793	29.00553637392183	21.58714869127237
40-41	21.806446352531225	27.605760471215422	28.934241711245683	21.65355146500767
42-43	21.820234651535188	27.780372533695818	28.62234510788711	21.777047706881884
44-45	21.80197166295612	27.907971878497605	28.455454728100516	21.83460173044576
46-47	21.96079163653497	27.839179165426504	28.153799976334753	22.046229221703776
48-49	22.007844640696224	28.005487884412943	28.041039168933978	21.945628305956856
50-51	21.97231006565494	28.08071821163311	27.96094926857979	21.986022454132158
52-53	22.0527015256153	28.040638514910437	27.928856275950775	21.977803683523486
54-55	22.063674031451587	28.02870306792375	27.851297158377548	22.056325742247115
56-57	22.10249682862797	28.156247378235154	27.73452164151882	22.00673415161805
58-59	21.943999559424824	28.23783036849718	27.890453789780512	21.92771628229748
60-61	22.11027125429498	28.273222925830666	27.707273291272717	21.909232528601635
62-63	22.033048328721378	28.387771209644498	27.68887937315524	21.890301088478886
64-65	22.061665147015223	28.2122939234942	27.743369990473887	21.98267093901669
66-67	22.082863207410373	28.218042581897436	27.602644369835172	22.096449840857023
68-69	22.065322234279165	28.15200831428948	27.66113936436023	22.121530087071125
70-71	22.03833124865139	28.016883292312173	27.737109034420936	22.207676424615503
72-73	22.114017465914827	28.04348027948469	27.665214918731955	22.177287335868524
74-75	21.984628281756606	28.151971828061832	27.63380632579996	22.2295935643816
76-77	22.08211192051658	28.10263686308499	27.59083680748316	22.22441440891527
78-79	21.98688655110183	28.15398957008679	27.596237816289644	22.262886062521734
80-81	22.038528696251568	28.120723041256856	27.627875452521778	22.212872809969795
82-83	22.14238950994571	28.03058552149104	27.72122129690664	22.10580367165661
84-85	22.145282097469288	28.050133849388892	27.69214300115409	22.11244105198773
86-87	22.03742756051728	28.112134922041548	27.6811365079681	22.16930100947307
88-89	22.111393661739058	28.035874771859344	27.584582913636197	22.268148652765404
90-91	22.113240238022335	28.12236476816642	27.655384097752826	22.109010896058418
92-93	22.128474227922755	28.078814589762658	27.70171984055811	22.090991341756478
94-95	21.936336384644754	28.16322847164885	27.75026884822041	22.150166295485985
96-97	21.88605041461015	28.21153798457303	27.76449482047339	22.137916780343428
98-99	22.156594170353795	28.813983549110034	27.79251214760665	21.236910132929513
100-101	22.614042958299375	31.51235399761529	25.018009371560403	20.855593672524932
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3085.0
1	2611.5
2	2929.5
3	3912.0
4	5048.5
5	7282.5
6	9093.0
7	10799.5
8	12283.0
9	12519.0
10	11616.0
11	10651.5
12	11740.5
13	15787.0
14	25601.5
15	39015.5
16	49313.0
17	54343.0
18	54881.0
19	52275.0
20	48113.0
21	44067.5
22	42264.0
23	44707.5
24	51143.0
25	62396.0
26	77631.5
27	94568.5
28	114277.5
29	135355.0
30	155151.0
31	174564.0
32	194082.0
33	212564.0
34	229902.0
35	245611.0
36	262212.5
37	277590.0
38	289616.0
39	298952.0
40	308021.5
41	316829.0
42	320874.5
43	324277.5
44	328702.5
45	333544.0
46	338186.0
47	341489.0
48	343752.5
49	338293.5
50	327477.0
51	318091.5
52	302186.5
53	282087.0
54	264075.0
55	244774.0
56	224754.0
57	203133.5
58	179734.0
59	154458.0
60	128064.5
61	105339.5
62	84677.0
63	66352.0
64	51197.5
65	38184.0
66	29049.0
67	22035.0
68	15720.5
69	11120.5
70	8133.5
71	5973.0
72	4065.0
73	2826.0
74	2171.5
75	1554.5
76	998.5
77	571.5
78	400.0
79	304.0
80	194.5
81	112.0
82	78.5
83	61.5
84	49.5
85	37.5
86	27.0
87	18.0
88	10.0
89	6.5
90	6.0
91	5.0
92	4.5
93	2.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	6.347389767696312E-4
4	2.0359552085063646E-4
5	2.874289706126632E-4
6	3.113813848303852E-4
7	0.002670694185275996
8	8.143820834025458E-4
9	3.7126242037469E-4
10-11	6.586913909873533E-5
12-13	1.1976207108860969E-5
14-15	1.497025888607621E-4
16-17	0.0010000132935898908
18-19	1.796431066329145E-5
20-21	0.0
22-23	0.0029285433622947706
24-25	0.007110415284793098
26-27	0.03620787852754148
28-29	0.27989729723441537
30-31	0.24476862005296876
32-33	0.25227496238374336
34-35	0.13252181194995
36-37	0.02453617095059124
38-39	0.01585051345429594
40-41	0.00910910612697113
42-43	0.0060369185345621
44-45	0.006785796617971416
46-47	0.0014837715006978127
48-49	0.002088221453536692
50-51	0.0026502233325278106
52-53	0.0016774151342457914
54-55	2.5361287801974904E-4
56-57	0.0017579249453944564
58-59	0.0030844606066061735
60-61	0.013039660407696474
62-63	0.008351785566591132
64-65	0.002333509695868851
66-67	0.0015118938628517094
68-69	0.0022571530743570813
70-71	0.0037750907583041647
72-73	0.003416427900648292
74-75	0.0035149256557430266
76-77	0.0016706304966726607
78-79	9.502596109257048E-5
80-81	4.2877985148692346E-4
82-83	7.4787071858036185E-6
84-85	0.0
86-87	0.0
88-89	0.0
90-91	4.4022696530091754E-4
92-93	0.0076188652677472695
94-95	0.0011594616530355597
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	173.0
20-21	593.0
22-23	1345.0
24-25	3325.0
26-27	4559.0
28-29	7559.0
30-31	15946.0
32-33	21844.0
34-35	31571.0
36-37	42351.0
38-39	50935.0
40-41	54510.0
42-43	64954.0
44-45	77717.0
46-47	77496.0
48-49	68794.0
50-51	61341.0
52-53	60208.0
54-55	64831.0
56-57	68712.0
58-59	69089.0
60-61	68082.0
62-63	67298.0
64-65	71296.0
66-67	77436.0
68-69	73934.0
70-71	72351.0
72-73	69748.0
74-75	70605.0
76-77	72176.0
78-79	76219.0
80-81	78100.0
82-83	76607.0
84-85	77399.0
86-87	80773.0
88-89	86984.0
90-91	91906.0
92-93	98043.0
94-95	111203.0
96-97	169484.0
98-99	710404.0
100-101	5201988.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.53035481190197
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.9391555307804	76.92527884030483
2	7.064685355544128	12.084900901882744
3	1.6945388518811593	4.348035277318457
4	0.6264932123414698	2.143367469552566
5	0.2630201976308376	1.1248105413031058
6	0.13622126689545974	0.6990631974297281
7	0.08028219000995031	0.48065949376393075
8	0.0485701200950089	0.3323375683986241
9	0.03439294935572134	0.2647477045280359
>10	0.11037744206641273	1.436590536273318
>50	0.0019565305113456158	0.10795616654225046
>100	3.0635288806414135E-4	0.052252302702332186
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.1976207108860969E-5	0.0	1.1976207108860969E-5	0.0
3	0.0	1.1976207108860969E-5	0.0	2.3952414217721938E-5	0.0
4	0.0	4.7904828435443876E-5	0.0	2.3952414217721938E-5	0.0
5	0.0	5.988103554430484E-5	0.0	3.59286213265829E-5	0.0
6	0.0	5.988103554430484E-5	0.0	3.59286213265829E-5	0.0
7	0.0	5.988103554430484E-5	0.0	5.988103554430484E-5	0.0
8	0.0	5.988103554430484E-5	0.0	5.988103554430484E-5	0.0
9	0.0	1.0778586397974872E-4	0.0	5.988103554430484E-5	0.0
10-11	1.1976207108860969E-5	1.0778586397974872E-4	0.0	7.18572426531658E-5	0.0
12-13	1.1976207108860969E-5	1.3173827819747065E-4	0.0	1.0778586397974872E-4	0.0
14-15	1.1976207108860969E-5	1.8563121018734503E-4	0.0	1.1976207108860969E-4	1.7964310663291454E-5
16-17	1.1976207108860969E-5	1.916193137417755E-4	0.0	1.1976207108860969E-4	2.3952414217721938E-5
18-19	1.1976207108860969E-5	1.9760741729620598E-4	0.0	1.1976207108860969E-4	2.3952414217721938E-5
20-21	1.1976207108860969E-5	2.0359552085063646E-4	0.0	1.1976207108860969E-4	2.3952414217721938E-5
22-23	1.1976207108860969E-5	2.2155983151392791E-4	0.0	1.2575017464304018E-4	2.3952414217721938E-5
24-25	1.1976207108860969E-5	2.4551224573164985E-4	0.0	1.7365500307848402E-4	2.3952414217721938E-5
26-27	1.1976207108860969E-5	2.634765563949413E-4	0.0	2.754527635038023E-4	2.3952414217721938E-5
28-29	1.1976207108860969E-5	2.634765563949413E-4	0.0	4.491077665822863E-4	2.3952414217721938E-5
30-31	1.1976207108860969E-5	2.874289706126632E-4	0.0	0.0010060013971443214	2.3952414217721938E-5
32-33	1.1976207108860969E-5	2.874289706126632E-4	0.0	0.002389253318217763	2.3952414217721938E-5
34-35	1.1976207108860969E-5	3.2335759193924613E-4	0.0	0.004616827840465904	2.3952414217721938E-5
36-37	1.1976207108860969E-5	3.6527431682025955E-4	0.0	0.00754501047858241	2.3952414217721938E-5
38-39	1.1976207108860969E-5	4.071910417012729E-4	0.0	0.012083992972840716	2.3952414217721938E-5
40-41	1.1976207108860969E-5	4.1916724881013387E-4	0.0	0.016694832709752187	2.3952414217721938E-5
42-43	1.1976207108860969E-5	4.491077665822863E-4	0.0	0.02127573192889151	2.3952414217721938E-5
44-45	1.1976207108860969E-5	5.030006985721607E-4	0.0	0.026665025127878945	2.3952414217721938E-5
46-47	1.1976207108860969E-5	5.269531127898826E-4	0.0	0.032641152475200566	3.59286213265829E-5
48-49	1.1976207108860969E-5	5.269531127898826E-4	0.0	0.03860530361541333	3.59286213265829E-5
50-51	1.1976207108860969E-5	6.467151838784923E-4	0.0	0.04412034698904381	3.59286213265829E-5
52-53	1.1976207108860969E-5	6.467151838784923E-4	0.0	0.04997072416172239	4.790482843544387E-5
54-55	1.1976207108860969E-5	6.527032874329228E-4	0.0	0.05601870875169718	5.988103554430484E-5
56-57	1.1976207108860969E-5	6.586913909873533E-4	0.0	0.06163554988575297	5.988103554430484E-5
58-59	1.1976207108860969E-5	6.586913909873533E-4	0.0	0.06685717618521636	5.988103554430484E-5
60-61	1.1976207108860969E-5	6.586913909873533E-4	0.0	0.07225844559131264	6.586913909873533E-5
62-63	1.1976207108860969E-5	6.646794945417837E-4	0.0	0.07740821464812286	7.18572426531658E-5
64-65	1.1976207108860969E-5	7.125843229772276E-4	0.0	0.08253403129071535	7.18572426531658E-5
66-67	1.1976207108860969E-5	7.305486336405191E-4	0.0	0.08800715793946481	7.18572426531658E-5
68-69	1.1976207108860969E-5	7.664772549671019E-4	0.0	0.09361801096996619	7.18572426531658E-5
70-71	1.1976207108860969E-5	7.784534620759629E-4	0.0	0.09867795847345995	7.18572426531658E-5
72-73	1.1976207108860969E-5	7.784534620759629E-4	0.0	0.1029295119971056	7.18572426531658E-5
74-75	1.1976207108860969E-5	8.024058762936849E-4	0.0	0.10667806482217906	7.18572426531658E-5
76-77	1.1976207108860969E-5	8.562988082835591E-4	0.0	0.1104146414401437	7.18572426531658E-5
78-79	1.1976207108860969E-5	8.742631189468506E-4	0.0	0.11339671701025009	7.18572426531658E-5
80-81	1.1976207108860969E-5	8.742631189468506E-4	0.0	0.11482188565620453	7.784534620759628E-5
82-83	1.1976207108860969E-5	8.742631189468506E-4	0.0	0.11522308859435137	8.383344976202677E-5
84-85	1.1976207108860969E-5	8.862393260557115E-4	0.0	0.11549854135785517	8.383344976202677E-5
86-87	1.1976207108860969E-5	8.982155331645726E-4	0.0	0.11561231532538935	8.383344976202677E-5
88-89	1.1976207108860969E-5	9.221679473822946E-4	0.0	0.11565423205027037	8.383344976202677E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10895	0.0	48.964127	1
GTATCAA	19210	0.0	37.50078	1
ATCAACG	24415	0.0	29.034607	3
TCAACGC	24565	0.0	28.839941	4
CAACGCA	25045	0.0	28.253128	5
TATCAAC	25485	0.0	28.100094	2
AACGCAG	25750	0.0	27.579039	6
ACGCAGA	29605	0.0	23.886795	7
CGCAGAG	31100	0.0	22.820465	8
GTGGTAT	5545	0.0	21.011465	1
GTACATG	23835	0.0	20.913317	1
TACATGG	23750	0.0	20.467299	2
GCAGAGT	34720	0.0	20.268818	9
CTTATAC	5205	0.0	20.170172	1
ACATGGG	23960	0.0	19.664608	3
AGAGTAC	31750	0.0	18.986227	10-11
TGGTATC	5630	0.0	18.875193	2
GAGTACT	21740	0.0	18.669127	12-13
CAGAGTA	33845	0.0	18.03165	10-11
CATGGGG	18085	0.0	17.226912	4
>>END_MODULE