FastQCFastQC Report
Fri 27 May 2016
SRR2139018_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139018_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13298160
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA191000.051.014221
GTATCAA352050.037.3212131
ATCAACG453900.028.2824923
TCAACGC455850.028.1985844
TATCAAC462850.028.0107142
CAACGCA462900.027.7691145
AACGCAG478500.026.9258546
ACGCAGA550700.023.3648517
CGCAGAG559400.022.978258
GTACATG439750.020.3793961
GCAGAGT640100.019.9684359
GTGGTAT91550.019.6801361
TACATGG448650.019.5679282
GAGTACT369400.019.00304212-13
AGAGTAC583050.018.94497310-11
ACATGGG453450.018.8455923
TGGTATC93750.018.4478322
CAGAGTA626850.017.83814610-11
AGTACTT410750.017.23484612-13
CATGGGG328250.017.0492344
Produced by FastQC (version 0.11.5)