##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139018_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13298160
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.42054171404164	34.0	31.0	34.0	31.0	34.0
2	32.578827446804674	34.0	31.0	34.0	31.0	34.0
3	32.56945276639776	34.0	31.0	34.0	31.0	34.0
4	36.15709481612494	37.0	35.0	37.0	35.0	37.0
5	36.08856571134653	37.0	35.0	37.0	35.0	37.0
6	36.113337108291674	37.0	36.0	37.0	35.0	37.0
7	36.060702683679544	37.0	35.0	37.0	35.0	37.0
8	36.04627121346111	37.0	35.0	37.0	35.0	37.0
9	37.73435460244124	39.0	38.0	39.0	35.0	39.0
10-11	37.671854038453446	39.0	37.0	39.0	35.0	39.0
12-13	37.63627095778664	39.0	37.0	39.0	35.0	39.0
14-15	38.946479475355986	40.0	38.0	41.0	35.0	41.0
16-17	38.92102351001943	40.0	38.0	41.0	35.0	41.0
18-19	38.84780604985953	40.0	38.0	41.0	35.0	41.0
20-21	38.90356215742568	40.0	38.0	41.0	35.0	41.0
22-23	38.82925168199422	40.0	38.0	41.0	35.0	41.0
24-25	38.74546891981905	40.0	38.0	41.0	34.5	41.0
26-27	38.65859059803866	40.0	38.0	41.0	34.5	41.0
28-29	38.44189880153094	40.0	38.0	41.0	34.0	41.0
30-31	38.36022168230463	40.0	38.0	41.0	34.0	41.0
32-33	38.28749713892418	40.0	38.0	41.0	34.0	41.0
34-35	38.097287639653814	40.0	38.0	41.0	33.0	41.0
36-37	38.22212909502562	40.0	38.0	41.0	34.0	41.0
38-39	38.341245015257165	40.0	38.0	41.0	34.0	41.0
40-41	38.33489826847905	40.0	38.0	41.0	34.0	41.0
42-43	38.20711614563725	40.0	38.0	41.0	33.0	41.0
44-45	38.13766137594604	40.0	38.0	41.0	33.0	41.0
46-47	38.14523050222441	40.0	38.0	41.0	33.0	41.0
48-49	38.01429269603692	40.0	37.0	41.0	33.0	41.0
50-51	37.94394831003882	40.0	37.0	41.0	33.0	41.0
52-53	37.86152293757484	40.0	37.0	41.0	33.0	41.0
54-55	37.74181339294833	40.0	36.5	41.0	33.0	41.0
56-57	37.57589093504797	39.5	36.0	41.0	32.5	41.0
58-59	37.408844365042775	39.0	36.0	41.0	32.0	41.0
60-61	37.21673964618098	39.0	35.0	40.5	32.0	41.0
62-63	36.98879941171214	38.5	35.0	40.0	32.0	41.0
64-65	36.73784346996789	38.0	35.0	40.0	31.0	41.0
66-67	36.45020799300543	37.5	35.0	40.0	31.0	41.0
68-69	36.13494977950891	37.0	35.0	40.0	31.0	41.0
70-71	35.83727457279264	36.5	34.5	39.0	31.0	41.0
72-73	35.511355438563534	36.0	34.0	39.0	30.5	40.0
74-75	35.189424652884824	35.5	34.0	38.5	30.0	40.0
76-77	34.19563447142793	35.0	33.0	37.0	29.0	39.0
78-79	34.528675855924945	35.0	34.0	37.0	30.0	39.0
80-81	34.39632752007807	35.0	34.0	36.5	30.0	39.0
82-83	34.15292307225684	35.0	34.0	36.0	30.0	37.5
84-85	33.898349445512764	35.0	34.0	36.0	30.0	37.0
86-87	33.66573518603245	35.0	33.0	35.0	29.5	37.0
88-89	33.433257239386926	35.0	33.0	35.0	29.0	36.0
90-91	33.321138615566056	35.0	33.0	35.0	29.0	36.0
92-93	33.25694452664116	35.0	33.0	35.0	29.0	36.0
94-95	33.176368837957	34.5	33.0	35.0	29.0	35.5
96-97	33.1740316219141	34.0	33.0	35.0	29.0	35.0
98-99	33.220381539757106	34.0	33.0	35.0	29.0	35.0
100-101	31.980848673639713	33.0	31.0	34.5	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	4.0
19	17.0
20	84.0
21	521.0
22	2264.0
23	7608.0
24	18432.0
25	37462.0
26	65204.0
27	100724.0
28	143023.0
29	188746.0
30	240129.0
31	300055.0
32	377097.0
33	488860.0
34	675691.0
35	975091.0
36	1635053.0
37	3022662.0
38	3491211.0
39	1469886.0
40	58335.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.29074209305846	21.871256295139244	15.68597813967755	24.152023472124746
2	13.80435649842305	22.223056924354253	40.90392375424655	23.068662822976147
3	16.78171948688891	27.338727840341043	31.506497735201922	24.373054937568124
4	12.065873302224208	19.132150420502605	38.94844193929765	29.85353433797554
5	12.77298804115773	38.364181075355816	35.67549824749532	13.187332635991131
6	29.082926835137446	38.885168232651026	18.770704035724716	13.261200896486816
7	25.61735142201933	33.27542908365021	23.652291748682018	17.454927745648437
8	24.413556803002777	36.704899973988745	21.227746147924538	17.653797075083936
9	25.53100210999893	16.61101339882033	21.49616588401165	36.36181860716909
10-11	23.914646594895505	26.998140454998033	29.089135022624017	19.998077927482445
12-13	24.854113220969374	24.783385364360694	29.201554651754325	21.160946762915607
14-15	22.008461261355023	25.986594135696695	26.978026355649632	25.026918247298653
16-17	21.33231468617211	29.076236445407194	27.60513879142771	21.986310076992986
18-19	21.44496548149881	28.123217210864198	29.568375952204207	20.863441355432784
20-21	22.26761907945421	27.157451057856736	29.508792351559755	21.066137511129305
22-23	22.02411412422342	27.161707771928445	29.33330565606116	21.48087244778698
24-25	21.740739576042127	27.386154834120056	29.364715446518364	21.508390143319453
26-27	21.682723735871406	27.49899926423241	29.32208499052943	21.49619200936675
28-29	21.609670944463897	27.420275247099934	29.307852111035505	21.66220169740066
30-31	21.821913172334906	27.409357519776158	29.27848694568117	21.49024236220777
32-33	21.6672066797421	27.565986113862934	29.186354801163095	21.58045240523187
34-35	21.79499376635275	27.605494228119852	29.16021888360017	21.43929312192723
36-37	21.831565214886613	27.560593930118475	29.09789412095115	21.509946734043755
38-39	21.69606620816561	27.661445283406227	29.098366661247827	21.544121847180335
40-41	21.818500361178753	27.503788911427264	29.061643740672118	21.61606698672187
42-43	21.825671459235856	27.666812963207736	28.701577861718587	21.805937715837818
44-45	21.906488274640466	27.830027974750326	28.44870801390543	21.814775736703776
46-47	22.0360721303422	27.802509012813154	28.16033669451603	22.00108216232862
48-49	22.02171677911575	27.964908026266983	27.995480470218965	22.017894724398303
50-51	22.014101909223584	27.98902464977503	27.909216813892947	22.087656627108444
52-53	22.078653036075064	27.997826948278053	27.806882841208676	22.116637174438207
54-55	22.107029064693233	27.921236799080162	27.799539452075777	22.172194684150824
56-57	22.109032419852355	28.056108144148144	27.722304160620403	22.112555275379098
58-59	21.98374330850083	28.131205668290526	27.83178109877143	22.053269924437213
60-61	22.206497861919374	28.08655929405961	27.741389485902744	21.96555335811827
62-63	22.09880907156354	28.165566206834598	27.701555157686915	22.034069563914947
64-65	22.09773859667091	28.077697894753534	27.684083184811474	22.140480323764084
66-67	22.151880567394493	28.040824799665252	27.560956550105715	22.246338082834537
68-69	22.057625781801242	27.977386906086178	27.68923349126166	22.275753820850916
70-71	22.10124617289364	27.83321327592519	27.73376557539172	22.331774975789457
72-73	22.20290796187374	27.893755243934205	27.625959713172282	22.27737708101977
74-75	22.03627263356946	27.977768890209926	27.667504308138312	22.318454168082305
76-77	22.1831877363201	27.86501162842223	27.64010866898763	22.31169196627004
78-79	22.120860674175887	27.814788226782188	27.628908153943815	22.435442945098107
80-81	22.131661714714618	27.888594782775606	27.619292342463993	22.360451160045784
82-83	22.15850376879175	27.827834497256244	27.74509695285087	22.268564781101137
84-85	22.23578018853985	27.81570183499038	27.645036385967703	22.30348159050207
86-87	22.135429503995134	27.83294134228349	27.702621943925603	22.329007209795776
88-89	22.16793977632645	27.68045265158258	27.70683717034063	22.44477040175034
90-91	22.197535876584578	27.75750232657116	27.7464499575876	22.298511839256665
92-93	22.26612011679323	27.695477905449028	27.70365318756231	22.334748790195434
94-95	22.111103755659244	27.650786475944393	27.831851267246666	22.406258501149694
96-97	22.11102319317419	27.718455611870557	27.80569238279523	22.364828812160027
98-99	22.27122967693407	28.31098229759661	27.86675584073743	21.55103218473189
100-101	22.726498669872303	30.99131370915299	25.05816571168806	21.224021909286652
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4178.0
1	3663.5
2	4666.5
3	6483.5
4	8348.0
5	11955.5
6	14951.5
7	18455.5
8	21854.0
9	22667.5
10	20936.0
11	18834.0
12	20469.0
13	27954.5
14	46411.5
15	71490.5
16	90827.0
17	99423.0
18	98439.5
19	91520.5
20	82184.5
21	73230.0
22	67950.0
23	70201.5
24	81469.0
25	101019.0
26	125798.0
27	152200.5
28	181945.5
29	214683.0
30	244802.5
31	274295.0
32	305952.5
33	338302.5
34	365897.5
35	389914.5
36	418500.5
37	445532.0
38	464111.0
39	476214.0
40	485321.5
41	494569.5
42	502197.0
43	511651.0
44	519970.5
45	526138.0
46	528557.5
47	526656.5
48	528085.5
49	523088.5
50	511520.0
51	498698.5
52	479875.0
53	456443.5
54	432768.0
55	405633.0
56	374983.0
57	339317.0
58	299429.0
59	257212.5
60	212994.5
61	173626.0
62	138324.5
63	106328.5
64	79279.0
65	59467.0
66	45636.5
67	35097.5
68	26908.0
69	19354.5
70	13493.0
71	9722.0
72	6836.5
73	4644.5
74	3077.5
75	1966.0
76	1316.5
77	869.5
78	578.5
79	391.0
80	250.5
81	155.5
82	117.5
83	85.5
84	57.0
85	44.0
86	38.0
87	32.5
88	24.0
89	13.0
90	6.0
91	5.0
92	4.5
93	3.5
94	2.5
95	2.0
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.2559511992636576E-5
2	2.2559511992636576E-5
3	4.887894265071257E-4
4	1.955157706028503E-4
5	3.7599186654394294E-4
6	2.1055544526460805E-4
7	0.0025943438791532062
8	5.188687758306412E-4
9	3.684720292130641E-4
10-11	1.0903764129774344E-4
12-13	3.759918665439429E-5
14-15	1.8423601460653205E-4
16-17	9.81338771679691E-4
18-19	1.1279755996318288E-5
20-21	0.0
22-23	0.0026473211666864717
24-25	0.006954689614158409
26-27	0.03497305035592435
28-29	0.27162119552034386
30-31	0.2363075005022265
32-33	0.2435059017098074
34-35	0.12712476514310544
36-37	0.021862612406809995
38-39	0.01346940711655991
40-41	0.006915055245747049
42-43	0.004308813726957494
44-45	0.004879670342463247
46-47	9.094382929511098E-4
48-49	0.0014337491858281521
50-51	0.0019178009559512547
52-53	9.503051332315612E-4
54-55	7.36889236009206E-5
56-57	0.0011439857181023431
58-59	0.002333892592345774
60-61	0.010457195221238454
62-63	0.0062319938678538815
64-65	0.0017312370966056866
66-67	0.0016148173213011983
68-69	0.0027393253610505214
70-71	0.0044720932753916365
72-73	0.00393880342370987
74-75	0.004089158290368555
76-77	0.001767872655792864
78-79	6.468603868751842E-5
80-81	5.472170947252902E-4
82-83	1.8941741926153537E-5
84-85	0.0
86-87	9.713366802033766E-6
88-89	0.0
90-91	5.54864834926212E-4
92-93	0.00836765877022851
94-95	0.0011326118302908915
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	302.0
20-21	991.0
22-23	2074.0
24-25	5748.0
26-27	7694.0
28-29	12001.0
30-31	26123.0
32-33	34385.0
34-35	49609.0
36-37	68161.0
38-39	83391.0
40-41	90458.0
42-43	108078.0
44-45	130532.0
46-47	129768.0
48-49	113297.0
50-51	100005.0
52-53	96424.0
54-55	104720.0
56-57	109772.0
58-59	110321.0
60-61	109706.0
62-63	108488.0
64-65	115635.0
66-67	123529.0
68-69	122829.0
70-71	121334.0
72-73	117435.0
74-75	120066.0
76-77	121517.0
78-79	130335.0
80-81	132320.0
82-83	129838.0
84-85	132258.0
86-87	138870.0
88-89	148602.0
90-91	159382.0
92-93	168495.0
94-95	188327.0
96-97	276388.0
98-99	1128587.0
100-101	8120365.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.02330892438331
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.65878450496432	71.02404776881667
2	7.850207695510004	12.720996064677564
3	2.3209445065229426	5.641518112450762
4	0.9561630285528406	3.0988596977804295
5	0.45108480625765235	1.8274191804254678
6	0.2601358902253277	1.2646242357627715
7	0.1467804560571388	0.83248467646256
8	0.09201796961970084	0.5964480303273231
9	0.06667362036524892	0.4861905605964556
>10	0.19433644152437565	2.288604256842777
>50	0.002502121286268744	0.1388539911522589
>100	2.9923662804315614E-4	0.04587541263345623
>500	6.972248617652728E-5	0.03407801207142401
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.759918665439429E-5	0.0	0.0	0.0
3	0.0	3.759918665439429E-5	0.0	0.0	0.0
4	0.0	8.271821063966744E-5	0.0	0.0	0.0
5	0.0	9.02380479705463E-5	0.0	0.0	0.0
6	0.0	9.02380479705463E-5	0.0	0.0	7.519837330878859E-6
7	0.0	9.775788530142517E-5	0.0	0.0	7.519837330878859E-6
8	7.519837330878859E-6	1.278372346249406E-4	0.0	0.0	1.5039674661757717E-5
9	7.519837330878859E-6	1.8799593327197147E-4	0.0	0.0	2.2559511992636576E-5
10-11	2.2559511992636576E-5	1.8799593327197147E-4	0.0	0.0	2.2559511992636576E-5
12-13	2.2559511992636576E-5	1.8799593327197147E-4	0.0	0.0	3.0079349323515434E-5
14-15	2.6319430658076003E-5	2.857538185733966E-4	0.0	0.0	6.391861731247029E-5
16-17	3.759918665439429E-5	2.932736559042755E-4	0.0	7.519837330878859E-6	8.271821063966744E-5
18-19	5.263886131615201E-5	3.346327612241092E-4	0.0	7.519837330878859E-6	1.015178039668646E-4
20-21	9.02380479705463E-5	3.7975178520938233E-4	0.0	7.519837330878859E-6	1.0527772263230401E-4
22-23	1.2031739729406174E-4	4.2863072786009494E-4	0.0	7.519837330878859E-6	1.3911699062125888E-4
24-25	1.541566652830166E-4	4.6998983317992867E-4	0.0	1.5039674661757717E-5	1.6543642127933488E-4
26-27	1.9551577060285033E-4	4.887894265071258E-4	0.0	5.263886131615201E-5	1.7295625861021374E-4
28-29	1.9551577060285033E-4	5.000691825034441E-4	0.0	2.5943438791532064E-4	1.8799593327197147E-4
30-31	1.9551577060285033E-4	6.015869864703086E-4	0.0	9.36219747694418E-4	1.9927568926828975E-4
32-33	1.9551577060285033E-4	6.053469051357481E-4	0.0	0.002270990873925415	2.180752825954869E-4
34-35	1.9551577060285033E-4	7.068647091026128E-4	0.0	0.0042111089052921605	2.331149572572446E-4
36-37	1.9927568926828975E-4	8.121424317349168E-4	0.0	0.007106246277680521	2.331149572572446E-4
38-39	2.0303560793372917E-4	8.610213743856293E-4	0.0	0.011200797704344059	2.4439471325356287E-4
40-41	2.4439471325356287E-4	9.662990970179333E-4	0.0	0.015915735710805103	2.556744692498812E-4
42-43	2.556744692498812E-4	0.0010001383650068882	0.0	0.020217082664067812	2.5943438791532064E-4
44-45	2.556744692498812E-4	0.0011317355182972682	0.0	0.02523281416376401	2.669542252461995E-4
46-47	2.707141439116389E-4	0.0012407731595950116	0.0	0.030970450047224582	2.7823398124251776E-4
48-49	2.707141439116389E-4	0.0012520529155913298	0.0	0.03661032804538372	2.932736559042755E-4
50-51	2.744740625770783E-4	0.0013648504755545128	0.0	0.0420058113302893	2.9703357456971487E-4
52-53	2.819938999079572E-4	0.0013798901502162706	0.0	0.047660729003110205	3.195930865623515E-4
54-55	2.8575381857339665E-4	0.0013949298248780283	0.0	0.053247968139953195	3.534323545513064E-4
56-57	2.8575381857339665E-4	0.0014212492555361043	0.0	0.05878256841548003	3.7599186654394294E-4
58-59	2.932736559042755E-4	0.0014362889301978619	0.0	0.06405773430309156	3.9479145987114005E-4
60-61	3.0831333056603315E-4	0.001481407954183135	0.0	0.06905842612812599	4.0607121586745833E-4
62-63	3.1583316789691204E-4	0.0015114873035066505	0.0	0.07379216372791424	4.361505651909738E-4
64-65	3.195930865623515E-4	0.001699483236778622	0.0	0.07875901628495972	4.361505651909738E-4
66-67	3.271129238932303E-4	0.0017521220980947741	0.0	0.08368074981801994	4.399104838564132E-4
68-69	3.421525985549881E-4	0.001804760959410926	0.0	0.08880175904034844	4.474303211872921E-4
70-71	3.459125172204275E-4	0.0018461200647307597	0.0	0.09380997070271377	4.925493451725652E-4
72-73	3.459125172204275E-4	0.0018536399020616387	0.0	0.09758492904281495	5.489481251541566E-4
74-75	3.459125172204275E-4	0.0018686795767233963	0.0	0.10094253641105236	5.489481251541566E-4
76-77	3.459125172204275E-4	0.0019589176246939425	0.0	0.1038639932140988	5.489481251541566E-4
78-79	3.459125172204275E-4	0.0019927568926828977	0.0	0.1066500929451894	5.489481251541566E-4
80-81	3.459125172204275E-4	0.0020002767300137765	0.0	0.10792846529143882	5.60227881150475E-4
82-83	3.459125172204275E-4	0.002015316404675534	0.0	0.10833453650730628	5.940671491394299E-4
84-85	3.459125172204275E-4	0.002026596160671852	0.0	0.1086240502445451	5.940671491394299E-4
86-87	3.459125172204275E-4	0.002082994940653444	0.0	0.10872932796717741	5.940671491394299E-4
88-89	3.459125172204275E-4	0.002225871849940142	0.0	0.10876692715383181	5.940671491394299E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	19100	0.0	51.01422	1
GTATCAA	35205	0.0	37.321213	1
ATCAACG	45390	0.0	28.282492	3
TCAACGC	45585	0.0	28.198584	4
TATCAAC	46285	0.0	28.010714	2
CAACGCA	46290	0.0	27.769114	5
AACGCAG	47850	0.0	26.925854	6
ACGCAGA	55070	0.0	23.364851	7
CGCAGAG	55940	0.0	22.97825	8
GTACATG	43975	0.0	20.379396	1
GCAGAGT	64010	0.0	19.968435	9
GTGGTAT	9155	0.0	19.680136	1
TACATGG	44865	0.0	19.567928	2
GAGTACT	36940	0.0	19.003042	12-13
AGAGTAC	58305	0.0	18.944973	10-11
ACATGGG	45345	0.0	18.845592	3
TGGTATC	9375	0.0	18.447832	2
CAGAGTA	62685	0.0	17.838146	10-11
AGTACTT	41075	0.0	17.234846	12-13
CATGGGG	32825	0.0	17.049234	4
>>END_MODULE