FastQCFastQC Report
Fri 27 May 2016
SRR2139017_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139017_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9142006
Sequences flagged as poor quality0
Sequence length19-101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA131250.048.1475371
CTTATAC83850.041.4685941
GTATCAA228450.036.036961
TATACAC111500.033.8605083
TTATACA117550.030.8852832
TCAACGC295850.027.4671554
ATCAACG295700.027.4090373
CAACGCA300250.027.05035
TATCAAC303900.026.8377342
AACGCAG308350.026.436316
TACACAT145700.025.5028765
ACACATC156350.022.8661966
ACGCAGA355900.022.8324537
ATACACA181350.022.463374
CGCAGAG363700.022.38948
ACATCTC161700.022.1621078
GTACATG296550.021.2664991
TACATGG302900.020.5253522
CACATCT173400.020.4703717
ACATGGG305000.019.8670963
Produced by FastQC (version 0.11.5)