FastQCFastQC Report
Fri 27 May 2016
SRR2139016_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139016_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20448993
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA233450.048.357421
GTATCAA438650.038.648131
TCAACGC523950.031.8366644
ATCAACG527100.031.6218173
CAACGCA535350.031.1506485
TATCAAC560850.030.0039442
AACGCAG557550.029.9557156
ACGCAGA642050.025.979617
CGCAGAG657650.025.383068
GCAGAGT775150.021.5019659
AGAGTAC706000.018.87171410-11
GAGTACT454900.018.78028712-13
GTACATG581500.018.640971
TACATGG574300.018.6038512
CAGAGTA764800.018.15514410-11
ACATGGG572300.018.1253323
GTGGTAT130600.018.0951331
TGGTATC131250.016.820512
GTACTTT518150.016.39203514-15
AGTACTT510350.016.16416412-13
Produced by FastQC (version 0.11.5)