##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139016_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20448993
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.480136601347557	33.0	32.0	34.0	25.0	34.0
2	31.911482389377316	33.0	32.0	33.0	28.0	34.0
3	32.54078848772651	33.0	33.0	34.0	31.0	34.0
4	32.851800086194956	33.0	33.0	34.0	32.0	34.0
5	32.98519531010647	33.0	33.0	34.0	32.0	34.0
6	36.61031753495148	38.0	37.0	38.0	34.0	38.0
7	37.091454283347844	38.0	38.0	38.0	36.0	38.0
8	37.24852627217389	38.0	38.0	38.0	36.0	38.0
9	37.24181073366302	38.0	38.0	38.0	36.0	38.0
10-11	37.3110802082039	38.0	38.0	38.0	37.0	38.0
12-13	37.325251639530606	38.0	38.0	38.0	37.0	38.0
14-15	37.340207706071396	38.0	38.0	38.0	37.0	38.0
16-17	37.301237645296276	38.0	38.0	38.0	37.0	38.0
18-19	37.2824264989479	38.0	38.0	38.0	37.0	38.0
20-21	37.34213245075932	38.0	38.0	38.0	37.0	38.0
22-23	37.355525254455515	38.0	38.0	38.0	37.0	38.0
24-25	37.35734018050091	38.0	38.0	38.0	37.0	38.0
26-27	37.26691612596486	38.0	38.0	38.0	37.0	38.0
28-29	37.18503059195496	38.0	38.0	38.0	36.5	38.0
30-31	37.25921076496209	38.0	38.0	38.0	37.0	38.0
32-33	37.280828201457965	38.0	38.0	38.0	37.0	38.0
34-35	37.2342891357885	38.0	38.0	38.0	37.0	38.0
36-37	37.23772246801657	38.0	38.0	38.0	37.0	38.0
38-39	37.2313905608598	38.0	38.0	38.0	37.0	38.0
40-41	37.187244660913386	38.0	38.0	38.0	36.5	38.0
42-43	37.17841863619621	38.0	38.0	38.0	36.0	38.0
44-45	37.106294712483255	38.0	38.0	38.0	36.0	38.0
46-47	37.07502763115069	38.0	38.0	38.0	36.0	38.0
48-49	37.045028206932415	38.0	38.0	38.0	36.0	38.0
50-51	37.032096973284126	38.0	38.0	38.0	36.0	38.0
52-53	37.00653814989279	38.0	38.0	38.0	36.0	38.0
54-55	36.96249962945592	38.0	38.0	38.0	36.0	38.0
56-57	36.9451827978436	38.0	38.0	38.0	36.0	38.0
58-59	36.948766231852105	38.0	38.0	38.0	36.0	38.0
60-61	36.95583178377735	38.0	38.0	38.0	36.0	38.0
62-63	36.890637962702286	38.0	38.0	38.0	35.5	38.0
64-65	36.914854594438	38.0	38.0	38.0	36.0	38.0
66-67	36.92450093175522	38.0	38.0	38.0	36.0	38.0
68-69	36.891285291043374	38.0	38.0	38.0	35.5	38.0
70-71	36.83985151116862	38.0	38.0	38.0	35.0	38.0
72-73	36.85479655169443	38.0	38.0	38.0	35.0	38.0
74-75	36.874201728489695	38.0	38.0	38.0	35.0	38.0
76-77	36.798888713150276	38.0	38.0	38.0	35.0	38.0
78-79	36.818321323793015	38.0	38.0	38.0	35.0	38.0
80-81	36.79093104481794	38.0	38.0	38.0	35.0	38.0
82-83	36.74986400761237	38.0	38.0	38.0	35.0	38.0
84-85	36.76361479073134	38.0	38.0	38.0	35.0	38.0
86-87	36.78005919161933	38.0	38.0	38.0	35.0	38.0
88-89	36.686684338752286	38.0	38.0	38.0	34.5	38.0
90-91	36.63507629306356	38.0	38.0	38.0	34.0	38.0
92-93	36.67896655276474	38.0	38.0	38.0	34.0	38.0
94-95	36.660684223861196	38.0	38.0	38.0	34.0	38.0
96-97	36.60874331498812	38.0	38.0	38.0	34.0	38.0
98-99	36.61883073887759	38.0	38.0	38.0	34.0	38.0
100	34.74807938722219	38.0	34.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	4.0
18	12.0
19	57.0
20	167.0
21	508.0
22	1616.0
23	4445.0
24	10021.0
25	19802.0
26	35285.0
27	57740.0
28	90209.0
29	132091.0
30	180932.0
31	240717.0
32	314821.0
33	425912.0
34	627266.0
35	1105470.0
36	2914672.0
37	1.4287246E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.044006078930146	21.375223709059902	14.340813750584196	24.239956461425756
2	13.691037837920838	22.103524659392594	41.53236373662202	22.67307376606455
3	18.00867651526899	26.677025122948596	30.700944540398638	24.61335382138377
4	12.550077678687266	18.473375274787532	39.64013659164728	29.33641045487792
5	13.215643854940733	38.115583395404244	35.481400073267395	13.187372676387628
6	29.085524407632267	38.67861716450328	19.166771276964333	13.069087150900113
7	25.73170314845702	33.12848682004457	23.7446543148459	17.39515571665251
8	23.886941523232956	36.972202005252775	21.5901291569712	17.550727314543067
9	25.31713419824634	17.180303206128535	21.69333228291486	35.80923031271027
10-11	23.664104144394788	27.11846739837018	29.403438105729705	19.81399035150533
12-13	24.638428405741056	24.698536500061397	29.461546101561087	21.20148899263646
14-15	21.826681147575336	26.012862833881357	27.149813685202005	25.010642333341305
16-17	21.323734621064226	29.130148364763	27.777035279927965	21.76908173424481
18-19	21.345532760464046	28.310257135889284	29.559714749767874	20.784495353878796
20-21	22.177777596294625	27.452761694988187	29.40600181678218	20.96345889193501
22-23	21.932835846077428	27.35924954674559	29.242468871020566	21.465445736156415
24-25	21.68298561005767	27.478024128293765	29.326585481296753	21.51240478035181
26-27	21.657946515405644	27.590831838454914	29.246713338045733	21.50450830809371
28-29	21.60986880721254	27.61355790490269	29.276545707103352	21.500027580781417
30-31	21.842172912533627	27.499758685445112	29.194214041983173	21.463854360038084
32-33	21.663895416928664	27.604199921908723	29.20365385183117	21.528250809331446
34-35	21.769964352394126	27.639173864903178	29.177658540138317	21.41320324256438
36-37	21.774220329351852	27.522896967363398	29.16603461005195	21.53684809323281
38-39	21.628310799649572	27.69850280920838	29.226957123787596	21.44622926735445
40-41	21.845504470306896	27.57883914852412	29.094876951217945	21.48077942995104
42-43	21.782311807654207	27.624964064880547	28.941854722951497	21.65086940451375
44-45	21.85461908901516	27.748223989582193	28.814491889105238	21.58266503229741
46-47	21.86219023601015	27.672153246512988	28.64652392750955	21.819132589967317
48-49	21.82435528140565	27.80378344372103	28.622686172413225	21.74917510246009
50-51	21.795479834017428	27.897450550382953	28.535082939093755	21.77198667650587
52-53	21.897182009454013	27.880016497356696	28.394087769134146	21.82871372405514
54-55	21.966420357724413	27.803306767227326	28.409066539461275	21.821206335586986
56-57	21.92793833434855	27.872463981665547	28.388296787655293	21.81130089633061
58-59	21.87069680129548	27.931676532180667	28.463632689921642	21.73399397660221
60-61	21.93752744729125	27.92245613782942	28.413211501443204	21.72680491343613
62-63	21.93328331316863	27.948037664871823	28.369111889804632	21.749567132154915
64-65	21.918962353525647	27.905238734106835	28.35789697054937	21.817901941818153
66-67	21.96455359135194	27.912155408103928	28.2737712909848	21.849519709559335
68-69	21.901304260897277	27.85789976067467	28.35877414069101	21.882021837737046
70-71	21.932705513052344	27.69451153912492	28.36128407183145	22.011498875991283
72-73	21.991466647011983	27.704969715714668	28.33520134174637	21.96836229552698
74-75	21.833484060998316	27.833858950292445	28.33697633093546	21.99568065777378
76-77	21.989510820432876	27.780334215525354	28.220948309227968	22.009206654813806
78-79	21.89870751675857	27.782683366131394	28.22971859030796	22.08889052680208
80-81	21.977026758661054	27.806750229656924	28.157219473049132	22.059003538632883
82-83	21.987499253243122	27.79431296330972	28.24651582065424	21.971671962792918
84-85	22.08958042813745	27.77679583224219	28.164685665440842	21.968938074179515
86-87	21.954329100420303	27.835621545943205	28.18270304015443	22.027346313482067
88-89	22.019835911782348	27.780861996932458	28.112612280584475	22.08668981070071
90-91	22.11421108945073	27.835959634593614	28.092040382183814	21.957788893771838
92-93	22.132375651114366	27.790017661407205	28.098369415444807	21.979237272033618
94-95	21.968632768243843	27.915744023953092	28.10877757953939	22.006845628263672
96-97	22.050749698651455	28.03700991100141	28.160857687402785	21.75138270294435
98-99	23.186308188482187	29.886438696443378	28.36076017572851	18.566492939345924
100	22.00502112995923	30.802039380015238	22.38745702891663	24.805482461108898
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3983.0
1	3591.0
2	4506.0
3	6487.5
4	8415.5
5	11280.0
6	13872.5
7	18162.5
8	23818.5
9	27193.5
10	27836.5
11	27977.5
12	30258.0
13	35891.0
14	47243.5
15	60382.5
16	69316.0
17	73023.5
18	71115.0
19	66043.5
20	61824.5
21	60991.0
22	66593.0
23	80293.5
24	102356.5
25	134405.0
26	176267.5
27	222378.0
28	269101.0
29	320583.5
30	372670.0
31	423526.0
32	475067.0
33	526297.5
34	577154.0
35	620187.0
36	664551.0
37	708059.0
38	736893.0
39	759048.0
40	784670.5
41	803975.0
42	810374.5
43	819503.0
44	832104.0
45	843660.5
46	844252.5
47	835026.5
48	823742.0
49	804171.0
50	778367.0
51	747791.0
52	709471.0
53	666143.5
54	623419.0
55	578401.0
56	529947.0
57	477572.0
58	423836.0
59	367179.0
60	306143.5
61	247276.5
62	192823.5
63	147035.5
64	110736.0
65	82295.0
66	61047.5
67	45998.5
68	34641.0
69	25307.5
70	18410.0
71	12984.5
72	8721.0
73	5796.0
74	3792.5
75	2282.0
76	1320.0
77	775.5
78	502.5
79	325.0
80	210.5
81	135.0
82	89.5
83	59.0
84	35.5
85	22.5
86	15.0
87	10.5
88	6.5
89	5.5
90	5.5
91	3.5
92	2.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	4.890216354419017E-5
3	0.0
4	5.868259625302821E-5
5	0.0029634711107779242
6	4.0099774106235937E-4
7	1.1736519250605642E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0010420656716146276
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	1.0016757032840238E-5
44-45	1.0082267013869695E-5
46-47	2.539823931277647E-5
48-49	8.445191169953791E-5
50-51	4.613555450683799E-4
52-53	2.023765755859645E-4
54-55	3.421828612672614E-4
56-57	4.920387472882942E-4
58-59	3.6852703799362637E-4
60-61	1.8700018523704062E-5
62-63	3.5795204599872184E-4
64-65	9.490249934137666E-5
66-67	1.1752919199637135E-4
68-69	4.961856965045455E-4
70-71	1.0288144226212824E-4
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	9.902176727187928E-4
90-91	0.0
92-93	6.13904067666962E-6
94-95	7.132731322539877E-5
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	115.0
20-21	461.0
22-23	934.0
24-25	1540.0
26-27	3773.0
28-29	10191.0
30-31	31236.0
32-33	40900.0
34-35	57830.0
36-37	91631.0
38-39	108062.0
40-41	107023.0
42-43	122758.0
44-45	147472.0
46-47	151043.0
48-49	141685.0
50-51	129970.0
52-53	127168.0
54-55	136757.0
56-57	143740.0
58-59	142507.0
60-61	140228.0
62-63	137054.0
64-65	143608.0
66-67	153805.0
68-69	156250.0
70-71	156992.0
72-73	145762.0
74-75	146655.0
76-77	152728.0
78-79	158193.0
80-81	157687.0
82-83	150108.0
84-85	147302.0
86-87	153567.0
88-89	160358.0
90-91	162823.0
92-93	162295.0
94-95	192463.0
96-97	629199.0
98-99	1441875.0
100-101	1.3903245E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.09882360938268
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.7313467103828	71.14909011878929
2	7.96398887558547	12.917402580963838
3	2.2499884713227027	5.474142544768334
4	0.8694575832421895	2.8204794871679395
5	0.42831414451434924	1.7367886627686426
6	0.2452450161206209	1.1933449382067869
7	0.14364247624321555	0.8154465090564436
8	0.09444396135086283	0.6127435330051983
9	0.0638327893024973	0.4659087708124306
>10	0.20574799521346285	2.4855788037566806
>50	0.0031113921235692696	0.16630864479134333
>100	8.209974271490482E-4	0.10196580397991586
>500	2.3850248291363347E-5	0.013684306303060641
>1k	3.57369227621779E-5	0.04711529563010541
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	4.890216354419017E-6	0.0	0.0	0.0	4.890216354419017E-6
3	4.890216354419017E-6	4.890216354419017E-6	0.0	0.0	4.890216354419017E-6
4	4.890216354419017E-6	2.4451081772095087E-5	0.0	0.0	1.4670649063257051E-5
5	4.890216354419017E-6	2.4451081772095087E-5	0.0	0.0	1.4670649063257051E-5
6	4.890216354419017E-6	2.4451081772095087E-5	0.0	0.0	1.9560865417676068E-5
7	4.890216354419017E-6	2.4451081772095087E-5	0.0	0.0	1.9560865417676068E-5
8	9.780432708838034E-6	2.4451081772095087E-5	0.0	0.0	1.9560865417676068E-5
9	9.780432708838034E-6	2.9341298126514102E-5	0.0	4.890216354419017E-6	2.4451081772095087E-5
10-11	1.4670649063257051E-5	2.9341298126514102E-5	0.0	1.4670649063257053E-5	2.4451081772095087E-5
12-13	1.9560865417676068E-5	2.9341298126514102E-5	0.0	2.4451081772095087E-5	2.6896189949304595E-5
14-15	1.9560865417676068E-5	6.601792078465673E-5	0.0	2.9341298126514102E-5	4.890216354419017E-5
16-17	1.9560865417676068E-5	6.846302896186624E-5	0.0	2.9341298126514102E-5	5.8682596253028205E-5
18-19	1.9560865417676068E-5	8.068856984791378E-5	0.0	2.9341298126514102E-5	7.824346167070427E-5
20-21	1.9560865417676068E-5	8.80238943795423E-5	0.0	2.9341298126514102E-5	7.824346167070427E-5
22-23	2.4451081772095087E-5	9.780432708838035E-5	0.0	3.9121730835352136E-5	9.535921891117084E-5
24-25	2.9341298126514102E-5	1.1247497615163741E-4	0.0	4.401194718977115E-5	1.1736519250605641E-4
26-27	2.9341298126514102E-5	1.2225540886047544E-4	0.0	1.149200843288469E-4	1.1736519250605641E-4
28-29	2.9341298126514102E-5	1.2225540886047544E-4	0.0	2.7874233220188396E-4	1.1736519250605641E-4
30-31	2.9341298126514102E-5	1.3692605792373248E-4	0.0	6.944107223275005E-4	1.1736519250605641E-4
32-33	3.9121730835352136E-5	1.3692605792373248E-4	0.0	0.001726246373109913	1.2225540886047544E-4
34-35	3.9121730835352136E-5	1.8093800511350365E-4	0.0	0.003168860197663523	1.2225540886047544E-4
36-37	4.8902163544190174E-5	2.249499523032748E-4	0.0	0.0054281401534051095	1.2225540886047544E-4
38-39	4.8902163544190174E-5	2.298401686576938E-4	0.0	0.008743706841701202	1.2714562521489445E-4
40-41	4.8902163544190174E-5	2.542912504297889E-4	0.0	0.012369802268502903	1.3203584156931347E-4
42-43	4.8902163544190174E-5	2.616265749614174E-4	0.0	0.016252634053911603	1.34480949746523E-4
44-45	4.8902163544190174E-5	2.9096787308793154E-4	0.0	0.020277282113598454	1.3937116610094198E-4
46-47	5.379237989860919E-5	3.129738466828171E-4	0.0	0.025108815871764445	1.4426138245536102E-4
48-49	5.379237989860919E-5	3.129738466828171E-4	0.0	0.029977026252588575	1.5159670698698953E-4
50-51	5.868259625302821E-5	3.520955775181692E-4	0.0	0.03439533672880615	1.5159670698698953E-4
52-53	6.357281260744723E-5	3.520955775181692E-4	0.0	0.038764745041479545	1.6382224787303708E-4
54-55	6.601792078465673E-5	3.569857938725882E-4	0.0	0.04331020114291202	1.784928969362941E-4
56-57	7.090813713907575E-5	3.6187601022700725E-4	0.0	0.04818330174009058	1.8827332964513216E-4
58-59	7.335324531628526E-5	3.6187601022700725E-4	0.0	0.05330091315499008	1.956086541767607E-4
60-61	7.335324531628526E-5	3.716564429358453E-4	0.0	0.05824736699748491	1.956086541767607E-4
62-63	7.335324531628526E-5	3.716564429358453E-4	0.0	0.0628490605869932	2.0783419506280825E-4
64-65	7.335324531628526E-5	4.352292555432925E-4	0.0	0.0673969617966029	2.1027930324001774E-4
66-67	8.068856984791378E-5	4.4011947189771155E-4	0.0	0.07238253736993308	2.1272441141722724E-4
68-69	8.80238943795423E-5	4.4745479642934003E-4	0.0	0.07705269398840325	2.1516951959443676E-4
70-71	8.80238943795423E-5	4.572352291381781E-4	0.0	0.08124605451231755	2.1761462777164628E-4
72-73	9.535921891117084E-5	4.596803373153876E-4	0.0	0.08472299834030947	2.542912504297889E-4
74-75	1.0269454344279936E-4	4.767960945558542E-4	0.0	0.08724145976283526	2.542912504297889E-4
76-77	1.0269454344279936E-4	5.036922845051588E-4	0.0	0.08973547010358895	2.567363586069984E-4
78-79	1.0269454344279936E-4	5.183629335684158E-4	0.0	0.0913908083395598	2.591814667842079E-4
80-81	1.0269454344279936E-4	5.183629335684158E-4	0.0	0.09200208538386218	2.6651679131583643E-4
82-83	1.0269454344279936E-4	5.208080417456253E-4	0.0	0.09250333256019012	2.9830319761956E-4
84-85	1.0269454344279936E-4	5.354786908088824E-4	0.0	0.09274050805337944	2.9830319761956E-4
86-87	1.0269454344279936E-4	5.42814015340511E-4	0.0	0.09283097705593621	2.9830319761956E-4
88	1.0269454344279936E-4	5.72155313467025E-4	0.0	0.09284075748864504	2.9830319761956E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	23345	0.0	48.35742	1
GTATCAA	43865	0.0	38.64813	1
TCAACGC	52395	0.0	31.836664	4
ATCAACG	52710	0.0	31.621817	3
CAACGCA	53535	0.0	31.150648	5
TATCAAC	56085	0.0	30.003944	2
AACGCAG	55755	0.0	29.955715	6
ACGCAGA	64205	0.0	25.97961	7
CGCAGAG	65765	0.0	25.38306	8
GCAGAGT	77515	0.0	21.501965	9
AGAGTAC	70600	0.0	18.871714	10-11
GAGTACT	45490	0.0	18.780287	12-13
GTACATG	58150	0.0	18.64097	1
TACATGG	57430	0.0	18.603851	2
CAGAGTA	76480	0.0	18.155144	10-11
ACATGGG	57230	0.0	18.125332	3
GTGGTAT	13060	0.0	18.095133	1
TGGTATC	13125	0.0	16.82051	2
GTACTTT	51815	0.0	16.392035	14-15
AGTACTT	51035	0.0	16.164164	12-13
>>END_MODULE